Characterizing diverse orthologues of the cystic fibrosis transmembrane conductance regulator protein for structural studies.

Abstract:

:As an ion channel, the cystic fibrosis transmembrane conductance regulator (CFTR) protein occupies a unique niche within the ABC family. Orthologues of CFTR are extant throughout the animal kingdom from sharks to platypods to sheep, where the osmoregulatory function of the protein has been applied to differing lifestyles and diverse organ systems. In humans, loss-of-function mutations to CFTR cause the disease cystic fibrosis, which is a significant health burden in populations of white European descent. Orthologue screening has proved fruitful in the pursuit of high-resolution structural data for several membrane proteins, and we have applied some of the princples developed in previous studies to the expression and purification of CFTR. We have overexpressed this protein, along with evolutionarily diverse orthologues, in Saccharomyces cerevisiae and developed a purification to isolate it in quantities sufficient for structural and functional studies.

journal_name

Biochem Soc Trans

authors

Pollock NL,Rimington TL,Ford RC

doi

10.1042/BST20150081

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

894-900

issue

5

eissn

0300-5127

issn

1470-8752

pii

BST20150081

journal_volume

43

pub_type

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