Characterizing diverse orthologues of the cystic fibrosis transmembrane conductance regulator protein for structural studies.

abstract：:The mitotic spindle is the macromolecular machine utilized to accurately segregate chromosomes in cells. How this self-organized structure assembles is a key aspect of understanding spindle morphogenesis. In the present review, we focus on understanding mechanisms of spindle self-assembly and address how subcellular s...

journal_title：Biochemical Society transactions

authors： Weaver LN,Walczak CE

更新日期：2015-02-01 00:00:00

abstract：:Tandem repeats (TR) in proteins are common in nature and have several unique functions. They come in various forms that are frequently difficult to recognize from a sequence. A previously proposed structural classification has been recently implemented in the RepeatsDB database. This defines five main classes, mainly ...

journal_title：Biochemical Society transactions

authors： Paladin L,Tosatto SC

更新日期：2015-10-01 00:00:00

abstract：:In the present paper, we describe multiple levels of cross-talk between the PI3K (phosphoinositide 3-kinase)/Akt and Ras/MAPK (mitogen-activated protein kinase) signalling pathways. Experimental data and computer simulations demonstrate that cross-talk is context-dependent and that both pathways can activate or inhibi...

journal_title：Biochemical Society transactions

authors： Aksamitiene E,Kiyatkin A,Kholodenko BN

更新日期：2012-02-01 00:00:00

abstract：:CT responds to properties of PC-depleted membranes: increased negative charge density, which concentrates the enzyme at the membrane surface, and lipid packing perturbations, which create holes in the membrane surface into which the hydrophobic side chains of the amphipathic helix of domain M can intercalate. The PC-d...

journal_title：Biochemical Society transactions

authors： Cornell RB

更新日期：1998-08-01 00:00:00

abstract：:Trafficking within eukaryotic cells is a complex and highly regulated process; events such as recycling of plasma membrane receptors, formation of multivesicular bodies, regulated release of hormones and delivery of proteins to membranes all require directionality and specificity. The underpinning processes, including...

journal_title：Biochemical Society transactions

authors： Laidlaw KME,Livingstone R,Al-Tobi M,Bryant NJ,Gould GW

更新日期：2017-12-15 00:00:00

abstract：:The intestinal epithelium represents one of our most important interfaces with the external environment. It must remain tightly balanced to allow nutrient absorption, but maintain barrier function and immune homoeostasis, a failure of which results in chronic infection or debilitating inflammatory bowel disease (IBD)....

journal_title：Biochemical Society transactions

authors： Worthington JJ

更新日期：2015-08-01 00:00:00

abstract：:Mammalian peroxisomes degrade fatty carboxylates via two pathways, beta-oxidation and, as shown more recently, alpha-oxidation. The latter process consists of an activation step, followed by a hydroxylation at position 2 and cleavage of the 2-hydroxyacyl-CoA, generating formyl-CoA (precursor of formate/CO(2)) and, in ...

journal_title：Biochemical Society transactions

authors： Van Veldhoven PP,Casteels M,Mannaerts GP,Baes M

更新日期：2001-05-01 00:00:00

abstract：:Isolation and purification of the [NiFe] hydrogenase of Desulfovibrio vulgaris Miyazaki F under aerobic conditions leads to a mixture of two states, Ni-A (unready) and Ni-B (ready). The two states are distinguished by different activation times and different EPR spectra. HYSCORE and ENDOR data and DFT calculations sho...

journal_title：Biochemical Society transactions

authors： van Gastel M,Fichtner C,Neese F,Lubitz W

更新日期：2005-02-01 00:00:00

abstract：:Gene-poor human chromosomes are reproducibly found at the nuclear periphery in proliferating cells. There are a number of inner nuclear envelope proteins that may have roles in chromosome location and anchorage, e.g. emerin and A-type lamins. In the last decade, a number of diseases associated with tissue degeneration...

journal_title：Biochemical Society transactions

authors： Meaburn KJ,Levy N,Toniolo D,Bridger JM

更新日期：2005-12-01 00:00:00

abstract：:Numerous RNA molecules with new catalytic properties have been isolated from synthetic combinatorial libraries. A broad range of chemical reactions is catalysed, and nucleic acids can accelerate bond formation between small organic substrates. This review focuses on carbon-carbon bond formation accelerated by in vitro...

journal_title：Biochemical Society transactions

authors： Jäschke A,Stuhlmann F,Bebenroth D,Keiper S,Wombacher R

更新日期：2002-11-01 00:00:00

abstract：:cAMP is an essential signalling molecule whose concentration in cells is regulated by a wide range of hormones. A large number of diseases, including cancer and asthma, are linked to improper regulation of the cAMP signalling system, and manipulation of cAMP levels by pharmaceutical agents has proven therapeutic benef...

journal_title：Biochemical Society transactions

authors： Yarwood SJ

更新日期：2005-12-01 00:00:00

abstract：:RDCs (residual dipolar couplings) in NMR spectroscopy provide information about protein dynamics complementary to NMR relaxation methods, especially in the previously inaccessible time window between the protein correlation time tau(c) and 50 micros. For ubiquitin, new modes of motion of the protein backbone could be ...

journal_title：Biochemical Society transactions

authors： Lakomek NA,Lange OF,Walter KF,Farès C,Egger D,Lunkenheimer P,Meiler J,Grubmüller H,Becker S,de Groot BL,Griesinger C

更新日期：2008-12-01 00:00:00

abstract：:African trypanosomes have plastic genomes with extensive variability at the chromosome ends. The genes encoding the expressed major surface protein of the infective bloodstream form stages of Trypanosoma brucei and are located at telomeres. These telomeric expression-site transcription units are turning out to be surp...

journal_title：Biochemical Society transactions

authors： Rudenko G

更新日期：2000-10-01 00:00:00

abstract：:ATP-binding cassette (ABC) transporters play crucial roles in cellular processes, such as nutrient uptake, drug resistance, cell-volume regulation and others. Despite their importance, all proposed molecular models for transport are based on indirect evidence, i.e. functional interpretation of static crystal structure...

journal_title：Biochemical Society transactions

authors： Husada F,Gouridis G,Vietrov R,Schuurman-Wolters GK,Ploetz E,de Boer M,Poolman B,Cordes T

更新日期：2015-10-01 00:00:00

abstract：:The formation of complexes between growth factor receptors and members of a family of G-protein-coupled receptors whose natural ligands are S1P (sphingosine 1-phosphate) and LPA (lysophosphatidic acid) represents a new signalling entity. This receptor complex allows for integrated signalling in response to growth fact...

journal_title：Biochemical Society transactions

authors： Pyne NJ,Waters C,Moughal NA,Sambi BS,Pyne S

更新日期：2003-12-01 00:00:00

abstract：:A modification of the Structure Alignment Program (SAP), combined with a novel automatic method for the definition of structural elements, correctly identified the core folds of a variety of small beta/alpha proteins when compared with a series of ideal architectures. This approach opens the possibility of not just de...

journal_title：Biochemical Society transactions

authors： Taylor WR

更新日期：2000-02-01 00:00:00

abstract：:The respiratory complex I (electrogenic NADH:quinone oxidoreductase) has been considered to act exclusively as a H+ pump. This was questioned when the search for the NADH-driven respiratory Na+ pump in Klebsiella pneumoniae initiated by Peter Dimroth led to the discovery of a Na+-translocating complex in this enteroba...

journal_title：Biochemical Society transactions

authors： Steffen W,Steuber J

更新日期：2013-10-01 00:00:00

abstract：:Invertebrates use a wide range of peptides as transmitters and hormones to regulate complex behaviour, physiology and development. These animals, especially those that are amenable to genetic study and are the subject of genome-sequencing projects, provide powerful model systems for understanding the functions of pept...

journal_title：Biochemical Society transactions

authors： Isaac RE,Siviter RJ,Stancombe P,Coates D,Shirras AD

更新日期：2000-01-01 00:00:00

abstract：:The ESCRT (endosomal sorting complex required for transport) machinery consists of a number of cytosolic proteins that make up three functional subcomplexes: ESCRT-I, ESCRT-II and ESCRT-III. These proteins function in multivesicular body formation and cell division and are co-opted by enveloped retroviruses to facilit...

journal_title：Biochemical Society transactions

authors： Carlton JG,Martin-Serrano J

更新日期：2009-02-01 00:00:00

abstract：:Bacterial hydrogenases have been harnessed to the removal of heavy metals from solution by reduction to less soluble metal species. For Pd(II), its bioreduction results in the deposition of cell-bound Pd(0)-nanoparticles that are ferromagnetic and have a high catalytic activity. Hydrogenases can also be used synthetic...

journal_title：Biochemical Society transactions

authors： Macaskie LE,Baxter-Plant VS,Creamer NJ,Humphries AC,Mikheenko IP,Mikheenko PM,Penfold DW,Yong P

更新日期：2005-02-01 00:00:00

abstract：:A new method of monitoring the rate of reactions in solid-phase peptide synthesis is described. A conductivity detector in the reaction cell (patent applied for) enables the deprotection, washing and subsequent coupling stages to be examined in detail. The half-lives of the reactions can be calculated and hence the op...

journal_title：Biochemical Society transactions

authors： Fox JE

更新日期：1990-12-01 00:00:00

abstract：:Other than signalling receptors sustaining leucocyte recruitment during inflammatory reactions, the chemokine system includes 'silent' receptors with distinct specificity and tissue distribution. The best-characterized molecule of this subgroup is the CC chemokine receptor D6, which binds most inflammatory CC chemokin...

journal_title：Biochemical Society transactions

authors： Borroni EM,Buracchi C,de la Torre YM,Galliera E,Vecchi A,Bonecchi R,Mantovani A,Locati M

更新日期：2006-12-01 00:00:00

abstract：:NO (nitric oxide) is a signal molecule involved in diverse physiological processes in cells which can become very toxic under certain conditions determined by its rate of production and diffusion. Several studies have clearly shown the production of NO in early stages of rhizobia-legume symbiosis and in mature nodules...

journal_title：Biochemical Society transactions

authors： Sánchez C,Cabrera JJ,Gates AJ,Bedmar EJ,Richardson DJ,Delgado MJ

更新日期：2011-01-01 00:00:00

abstract：:Virtual screening is being routinely used as an integral part of today's hit-identification strategies for, on one hand, prioritizing large corporate screening collections and, on the other hand, to extend the scope of screening to external databases. A brief description of the essential elements required for virtual ...

journal_title：Biochemical Society transactions

authors： Mestres J

更新日期：2002-08-01 00:00:00

abstract：:AWAP IV constitutes the C-terminal domain of the larger 81 kDa protein AWAK [Avian WAP (whey acidic protein) domain- and Kunitz domain-containing], which is predicted, through conserved domain database searching, to contain at least four WAP domains and one Kunitz domain. RT (reverse transcription)-PCR analyses reveal...

journal_title：Biochemical Society transactions

authors： Townes CL,Milona P,Hall J

更新日期：2006-04-01 00:00:00

abstract：:Inflammation and dyslipidaemia both play important roles in the development of glomerular atherosclerosis in renal diseases. We have demonstrated that inflammatory mediators induced Scr (scavenger receptor) expression and the formation of foam cells, and that AP-1 (activator protein 1)/ets were necessary transcription...

journal_title：Biochemical Society transactions

authors： Ruan XZ,Moorhead JF,Fernando R,Wheeler DC,Powis SH,Varghese Z

更新日期：2004-02-01 00:00:00

abstract：:The mitochondrial outer membrane separates the intermembrane space from the cytosol. The whole exchange of metabolites, cations and information between mitochondria and the cell occurs through the outer membrane. Experimental evidence is reviewed supporting the hypothesis of dynamic ADP compartmentation within the int...

journal_title：Biochemical Society transactions

authors： Gellerich FN,Trumbeckaite S,Opalka JR,Seppet E,Rasmussen HN,Neuhoff C,Zierz S

更新日期：2000-02-01 00:00:00

abstract：:RNase A (1 mM) was incubated with glucose (0.4 M) at 37 degrees C for up to 14 days in phosphate buffer (0.2 M, pH 7.4), digested with trypsin and analysed by LC-MS. The major sites of fructoselysine formation were Lys(1), Lys(7), Lys(37) and Lys(41). Three of these sites (Lys(7), Lys(37) and Lys(41)) were also the ma...

journal_title：Biochemical Society transactions

authors： Cotham WE,Hinton DJ,Metz TO,Brock JW,Thorpe SR,Baynes JW,Ames JM

更新日期：2003-12-01 00:00:00

abstract：:In order for cells to divide in a proficient manner, they must first double their biomass, which is considered to be the main rate-limiting phase of cell proliferation. Cell growth requires an abundance of energy and biosynthetic precursors such as lipids and amino acids. Consequently, the energy and nutrient status o...

journal_title：Biochemical Society transactions

authors： Dunlop EA,Tee AR

更新日期：2013-08-01 00:00:00

abstract：:PABPs [poly(A)-binding proteins] bind to the poly(A) tail of eukaryotic mRNAs and are conserved in species ranging from yeast to human. The prototypical cytoplasmic member, PABP1, is a multifunctional RNA-binding protein with roles in global and mRNA-specific translation and stability, consistent with a function as a ...

journal_title：Biochemical Society transactions

authors： Smith RW,Blee TK,Gray NK

更新日期：2014-08-01 00:00:00