Characterizing diverse orthologues of the cystic fibrosis transmembrane conductance regulator protein for structural studies.


:As an ion channel, the cystic fibrosis transmembrane conductance regulator (CFTR) protein occupies a unique niche within the ABC family. Orthologues of CFTR are extant throughout the animal kingdom from sharks to platypods to sheep, where the osmoregulatory function of the protein has been applied to differing lifestyles and diverse organ systems. In humans, loss-of-function mutations to CFTR cause the disease cystic fibrosis, which is a significant health burden in populations of white European descent. Orthologue screening has proved fruitful in the pursuit of high-resolution structural data for several membrane proteins, and we have applied some of the princples developed in previous studies to the expression and purification of CFTR. We have overexpressed this protein, along with evolutionarily diverse orthologues, in Saccharomyces cerevisiae and developed a purification to isolate it in quantities sufficient for structural and functional studies.


Biochem Soc Trans


Pollock NL,Rimington TL,Ford RC




Has Abstract


2015-10-01 00:00:00














  • A new approach for the investigation of reperfusion-related brain injury.

    abstract::Effective stroke therapies require recanalization of occluded cerebral blood vessels; however, early reperfusion can cause BBB (blood-brain barrier) injury, leading to cerebral oedema and/or devastating brain haemorrhage. These complications of early reperfusion, which result from excess production of ROS (reactive ox...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Maier CM,Hsieh L,Crandall T,Narasimhan P,Chan PH

    更新日期:2006-12-01 00:00:00

  • Mammalian expression of transmembrane receptors for pharmaceutical applications.

    abstract::Three mammalian expression systems suitable for expressing recombinant receptors have been described. Each is suited to a different aspect of the study of receptors and their behaviour. IRES-based vectors are ideal for creating stable mammalian cell lines suitable for screening receptors using a signalling readout. Un...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Rhodes AD,Bevan N,Patel K,Lee M,Rees S

    更新日期:1998-11-01 00:00:00

  • Evolutionary selection for protein aggregation.

    abstract::Protein aggregation is being found to be associated with an increasing number of human diseases. Aggregation can lead to a loss of function (lack of active protein) or to a toxic gain of function (cytotoxicity associated with protein aggregates). Although potentially harmful, protein sequences predisposed to aggregati...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Sanchez de Groot N,Torrent M,Villar-Piqué A,Lang B,Ventura S,Gsponer J,Babu MM

    更新日期:2012-10-01 00:00:00

  • Regional differences in protein production by human adipose tissue.

    abstract::Human adipose tissue has an important protein secretory function. Cytokines, hormones, prohormones and enzymes are secreted from fat cells and act in an endocrine or paracrine fashion. The production of several of these proteins is affected by obesity; normally there is an increase in the obese state. Protein producti...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Arner P

    更新日期:2001-05-01 00:00:00

  • Receptor tyrosine kinase-GPCR signal complexes.

    abstract::The formation of complexes between growth factor receptors and members of a family of G-protein-coupled receptors whose natural ligands are S1P (sphingosine 1-phosphate) and LPA (lysophosphatidic acid) represents a new signalling entity. This receptor complex allows for integrated signalling in response to growth fact...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Pyne NJ,Waters C,Moughal NA,Sambi BS,Pyne S

    更新日期:2003-12-01 00:00:00

  • Chaperones and protein folding in the archaea.

    abstract::A survey of archaeal genomes for the presence of homologues of bacterial and eukaryotic chaperones reveals several interesting features. All archaea contain chaperonins, also known as Hsp60s (where Hsp is heat-shock protein). These are more similar to the type II chaperonins found in the eukaryotic cytosol than to the...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Large AT,Goldberg MD,Lund PA

    更新日期:2009-02-01 00:00:00

  • Networking in the endoplasmic reticulum.

    abstract::The network of the ER (endoplasmic reticulum) is set up by cytoskeletal control of the movement and remodelling of polygonal rings of tubules, bundles of tubules and cisternal regions. We have developed a new image analysis tool, persistency mapping, to understand the framework upon which the plant ER remodels. With t...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Griffing LR

    更新日期:2010-06-01 00:00:00

  • Plasticity of the alpha4betadelta GABAA receptor.

    abstract::The GABAR [GABA(A) (gamma-aminobutyric acid type A) receptor], which mediates most inhibition in the brain, is regulated homoeostatically to maintain an optimal level of neuronal excitability. In particular, the alpha(4)betadelta subtype of the GABAR plays a pivotal role in this regulation. This receptor, which is exp...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Shen H,Smith SS

    更新日期:2009-12-01 00:00:00

  • Genomic DNA amplification by the multiple displacement amplification (MDA) method.

    abstract::Large amounts of DNA are frequently required for use in detection assays and genomic analysis. The limited availability of DNA can be a critical obstacle to meeting research and clinical needs. DNA amplification methods are often required to generate sufficient material from small specimens or environmental samples wi...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Lasken RS

    更新日期:2009-04-01 00:00:00

  • Applications of bacterial hydrogenases in waste decontamination, manufacture of novel bionanocatalysts and in sustainable energy.

    abstract::Bacterial hydrogenases have been harnessed to the removal of heavy metals from solution by reduction to less soluble metal species. For Pd(II), its bioreduction results in the deposition of cell-bound Pd(0)-nanoparticles that are ferromagnetic and have a high catalytic activity. Hydrogenases can also be used synthetic...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Macaskie LE,Baxter-Plant VS,Creamer NJ,Humphries AC,Mikheenko IP,Mikheenko PM,Penfold DW,Yong P

    更新日期:2005-02-01 00:00:00

  • Neuropilin regulation of angiogenesis.

    abstract::Blood vessel formation during vertebrate development relies on a process called angiogenesis and is essential for organ growth and tissue viability. In addition, angiogenesis leads to pathological blood vessel growth in diseases with tissue ischaemia, such as neovascular eye disease and cancer. Neuropilin 1 (NRP1) is ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Lampropoulou A,Ruhrberg C

    更新日期:2014-12-01 00:00:00

  • BMP9 is a potential therapeutic agent for use in oral and maxillofacial bone tissue engineering.

    abstract::Oral and maxillofacial surgery is often challenging due to defective bone healing owing to the microbial environment of the oral cavity, the additional involvement of teeth and esthetic concerns. Insufficient bone volume as a consequence of aging and some oral and maxillofacial surgical procedures, such as tumor resec...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Liu L,Chen Y,Song D,Huang D

    更新日期:2020-06-30 00:00:00

  • Insight into stability of CotA laccase from the spore coat of Bacillus subtilis.

    abstract::The axial ligand of the catalytic mononuclear T1 copper site (Met(502)) of the CotA laccase was replaced by a leucine or phenylalanine residue to increase the redox potential of the enzyme. These mutations led to an increase in the redox potential by approx. 100 mV relative to the wild-type enzyme but the catalytic co...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Melo EP,Fernandes AT,Durão P,Martins LO

    更新日期:2007-12-01 00:00:00

  • The zDHHC family of S-acyltransferases.

    abstract::The discovery of the zDHHC family of S-acyltransferase enzymes has been one of the major breakthroughs in the S-acylation field. Now, more than a decade since their discovery, major questions centre on profiling the substrates of individual zDHHC enzymes (there are 24 ZDHHC genes and several hundred S-acylated protein...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Lemonidis K,Werno MW,Greaves J,Diez-Ardanuy C,Sanchez-Perez MC,Salaun C,Thomson DM,Chamberlain LH

    更新日期:2015-04-01 00:00:00

  • Dissecting metabolism using zebrafish models of disease.

    abstract::Zebrafish (Danio rerio) are becoming an increasingly powerful model organism to study the role of metabolism in disease. Since its inception, the zebrafish model has relied on unique attributes such as the transparency of embryos, high fecundity and conservation with higher vertebrates, to perform phenotype-driven che...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Salmi TM,Tan VWT,Cox AG

    更新日期:2019-02-28 00:00:00

  • The Escherichia coli RNA degradosome: structure, function and relationship in other ribonucleolytic multienzyme complexes.

    abstract::mRNA instability is an intrinsic property that permits timely changes in gene expression by limiting the lifetime of a transcript. The RNase e of Escherichia coli is a single-strand-specific endo-nuclease involved in the processing of rRNA and the degradation of mRNA. A nucleolytic multi-enzyme complex now known as th...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Carpousis AJ

    更新日期:2002-04-01 00:00:00

  • The ProteinChip Biomarker System from Ciphergen Biosystems: a novel proteomics platform for rapid biomarker discovery and validation.

    abstract::Protein biochips are emerging in two distinct formats. The first involves high-density immobilized arrays of recognition molecules (e.g. antibodies), where target binding is monitored indirectly (e.g. via fluorescence). This technology is in its infancy, being limited by the availability of suitable binding molecules ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Chapman K

    更新日期:2002-04-01 00:00:00

  • Chromatin modulation and gene regulation in plants: insight about PRC1 function.

    abstract::In plant and metazoan, Polycomb Group (PcG) proteins play key roles in regulating developmental processes by repression of gene expression. PcG proteins function as multi-protein complexes; among them the best characterized ones are Polycomb Repressive Complex 1 (PRC1) and PRC2. PRC2 catalyzes histone H3 lysine 27 tri...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Wang Q,Shen WH

    更新日期:2018-08-20 00:00:00

  • Biochemistry and functional significance of collagen cross-linking.

    abstract::The biophysical characteristics of vascular tissues are dependent largely on the properties of fibrillar collagens. Considering the predominant structural component, collagen type I, the present review describes the mechanisms of formation and maturation of lysyl oxidase-mediated cross-linking, leading to an understan...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Robins SP

    更新日期:2007-11-01 00:00:00

  • Mechanisms of hormone-induced desensitization of adenylate cyclase.

    abstract::Luteinizing hormone (LH) interacts with its plasma membrane receptor to activate the formation of cyclic AMP via the regulatory GTP binding protein (Gs). This is followed by a desensitization of that same hormonal response which is caused by an uncoupling of the LH receptor from Gs. The coupling between Gs and the ade...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Cooke BA,Platts EA,Abayasekera DR,Rose MP

    更新日期:1989-08-01 00:00:00

  • The tuberous sclerosis complex (TSC) pathway and mechanism of size control.

    abstract::We have identified three groups of growth-constraint genes using mosaic genetic screens in Drosophila melanogaster, including PTEN (phosphatase and tensin homologue deleted on chromosome 10), and the tuberous sclerosis complex (TSC) genes, Tsc1 and Tsc2. Our studies show that all three groups of genes participate in m...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Potter CJ,Pedraza LG,Huang H,Xu T

    更新日期:2003-06-01 00:00:00

  • Oxidative protein folding in the mammalian endoplasmic reticulum.

    abstract::Native disulphide bonds are essential for the structure and function of many membrane and secretory proteins. Disulphide bonds are formed, reduced and isomerized in the endoplasmic reticulum of mammalian cells by a family of oxidoreductases, which includes protein disulphide isomerase (PDI), ERp57, ERp72, P5 and PDIR....

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Jessop CE,Chakravarthi S,Watkins RH,Bulleid NJ

    更新日期:2004-11-01 00:00:00

  • Control of membrane fusion dynamics during regulated exocytosis.

    abstract::The study of regulated exocytosis uniquely allows the direct measurement of intracellular membrane fusion events in real time. We have exploited this to examine factors that regulate not only the extent but also the dynamics of single fusion/release events. The general strategy used has been to assess exocytosis in tr...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Burgoyne RD,Fisher RJ,Graham ME,Haynes LP,Morgan A

    更新日期:2001-08-01 00:00:00

  • Inductive activity of recombinant human growth and differentiation factor-5.

    abstract::Growth and differentiation factor-5 (GDF-5) is a divergent member of the transforming growth factor-beta/bone morphogenetic protein (BMP) superfamily that is required for proper skeletal patterning and development in the vertebrate limb. Based on the homology of GDF-5 with other bone-inducing BMP family members, the i...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Spiro RC,Liu L,Heidaran MA,Thompson AY,Ng CK,Pohl J,Poser JW

    更新日期:2000-01-01 00:00:00

  • Floral stem cells: from dynamic balance towards termination.

    abstract::During early flower development in Arabidopsis, floral stem cells proliferate and produce a sufficient amount of cells that are recruited for organogenesis. However, after the central organ primordia initiate, stem cell activity in the floral meristem is terminated to ensure the differentiation of a fixed number of fl...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Sun B,Ito T

    更新日期:2010-04-01 00:00:00

  • Formate and its role in hydrogen production in Escherichia coli.

    abstract::The production of dihydrogen by Escherichia coli and other members of the Enterobacteriaceae is one of the classic features of mixed-acid fermentation. Synthesis of the multicomponent, membrane-associated FHL (formate hydrogenlyase) enzyme complex, which disproportionates formate into CO(2) and H(2), has an absolute r...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Sawers RG

    更新日期:2005-02-01 00:00:00

  • Chromosome positioning is largely unaffected in lymphoblastoid cell lines containing emerin or A-type lamin mutations.

    abstract::Gene-poor human chromosomes are reproducibly found at the nuclear periphery in proliferating cells. There are a number of inner nuclear envelope proteins that may have roles in chromosome location and anchorage, e.g. emerin and A-type lamins. In the last decade, a number of diseases associated with tissue degeneration...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Meaburn KJ,Levy N,Toniolo D,Bridger JM

    更新日期:2005-12-01 00:00:00

  • Watching conformational dynamics of ABC transporters with single-molecule tools.

    abstract::ATP-binding cassette (ABC) transporters play crucial roles in cellular processes, such as nutrient uptake, drug resistance, cell-volume regulation and others. Despite their importance, all proposed molecular models for transport are based on indirect evidence, i.e. functional interpretation of static crystal structure...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Husada F,Gouridis G,Vietrov R,Schuurman-Wolters GK,Ploetz E,de Boer M,Poolman B,Cordes T

    更新日期:2015-10-01 00:00:00

  • Collagen gene structure.

    abstract::The collagens of vertebrates may be divided into three groups according to chain size and whether or not the helical domains are continuous. Present evidence suggests that, at least within one of these groups, similarity between collagens is reflected in the organization of the genes that encode them. Early evidence s...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Dalgleish R

    更新日期:1988-10-01 00:00:00

  • The role of triacylglycerol as a reservoir of polyunsaturated fatty acids for the rapid production of chloroplastic lipids in certain microalgae.

    abstract::Many microalgae are known to accumulate triacylglycerols (TAGs), especially under nitrate starvation. Generally, these TAGs are predominantly constructed of saturated and monounsaturated fatty acids. In contrast, the TAGs of the red microalga Porphyridium cruentum are rich in arachidonic acid (AA, 20:4 omega6) and eic...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Cohen Z,Khozin-Goldberg I,Adlerstein D,Bigogno C

    更新日期:2000-12-01 00:00:00