Abstract:
:As an ion channel, the cystic fibrosis transmembrane conductance regulator (CFTR) protein occupies a unique niche within the ABC family. Orthologues of CFTR are extant throughout the animal kingdom from sharks to platypods to sheep, where the osmoregulatory function of the protein has been applied to differing lifestyles and diverse organ systems. In humans, loss-of-function mutations to CFTR cause the disease cystic fibrosis, which is a significant health burden in populations of white European descent. Orthologue screening has proved fruitful in the pursuit of high-resolution structural data for several membrane proteins, and we have applied some of the princples developed in previous studies to the expression and purification of CFTR. We have overexpressed this protein, along with evolutionarily diverse orthologues, in Saccharomyces cerevisiae and developed a purification to isolate it in quantities sufficient for structural and functional studies.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Pollock NL,Rimington TL,Ford RCdoi
10.1042/BST20150081subject
Has Abstractpub_date
2015-10-01 00:00:00pages
894-900issue
5eissn
0300-5127issn
1470-8752pii
BST20150081journal_volume
43pub_type
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