Immunohistochemical localization of insulin in the chick embryo during development.


:Since an extrapancreatic source of insulin has been postulated in the chick embryo, insulin was sought by immunohistochemistry in the embryo before and after development of the pancreas and other tissues. Using for the first time an antiserum specific for chicken insulin, localization of insulin was investigated in whole embryos (Days 1-8) and in brain, kidney, liver, and pancreas (Days 9-21 and 30). Results indicate the presence of immunoreactive insulin in pancreatic islets from Day 5 onward with an increase in staining intensity during development. Insulin was not detected in other tissues of the early developing embryo or in brain, liver, or kidney in later development. These data suggest that although extrapancreatic insulin has been reported, concentrations are not high enough in tissues to be detected with a specific, homologous antiserum and sensitive immunohistochemical techniques.


Gen Comp Endocrinol


Bagnell CA,Baker NK,McMurtry JP




Has Abstract


1989-02-01 00:00:00














  • Insulin and insulin-like growth factor-1 modulate the lipolytic action of growth hormone by altering signal pathway linkages.

    abstract::Growth hormone (GH) has many actions in vertebrates, including the regulation of two disparate metabolic processes: growth promotion (anabolic) and the mobilization of stored lipids (catabolic). Our previous studies showed that GH stimulated IGF-1 production in hepatocytes from fed rainbow trout, but in cells from fas...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Bergan-Roller HE,Ickstadt AT,Kittilson JD,Sheridan MA

    更新日期:2017-07-01 00:00:00

  • The basic route of the nuclear translocation porcine growth hormone (GH)-growth hormone receptor (GHR) complex (pGH/GHR) in porcine hepatocytes.

    abstract::Traditional views suggest that growth hormone and the growth hormone receptor (GH/GHR complex) exert their functions only on the plasma membrane. This paradigm, however, has been challenged by recent new findings that the GH/GHR complex could translocate into cell nuclei where they could still exhibit important physio...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Hainan L,Huilin L,Khan MA,Xin Z,YuJiang Y,Hui Z,Naiquan Y

    更新日期:2018-09-15 00:00:00

  • Increase in ovarian alpha-inhibin gene expression and plasma immunoreactive inhibin level is correlated with a decrease in ovulation rate in the domestic hen.

    abstract::This study was designed to examine the relationship among ovulation rate, inhibin alpha-subunit gene expression, and plasma immunoreactive inhibin levels between hens laying at a high ovulation rate and hens laying at a low ovulation rate. A porcine inhibin alpha-subunit cDNA was used to examine the expression of inhi...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Wang SY,Johnson PA

    更新日期:1993-07-01 00:00:00

  • Sex differences in mammalian and chicken-II gonadotropin-releasing hormone immunoreactivity in musk shrew brain.

    abstract::Many vertebrates have more than one molecular form of gonadotropin-releasing hormone (GnRH) present in brain. In all cases documented to date, GnRH neurons located in the forebrain are critical players in the brain-pituitary-gonadal feedback axis although the details of how steroids regulate GnRH remain elusive. The f...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Rissman EF,Li X

    更新日期:1998-12-01 00:00:00

  • Urinary clearance of arginine vasotocin in Pekin ducks.

    abstract::To quantify the renal excretion of arginine vasotocin (AVT) in birds, synthetic AVT was infused into conscious Pekin ducks at rates of 0.03, 0.10, and 0.30 ng/kg/min for 30 min and the relationships between plasma and urine AVT concentrations were monitored by radioimmunoassay. As plasma AVT concentrations increased (...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Gray DA

    更新日期:1995-07-01 00:00:00

  • Seasonal variations of androgens, estrogens, and progesterone in the different lobules of the testis and in the plasma of Salamandra salamandra.

    abstract::Progesterone, 4-androstenedione, testosterone, dihydrotestosterone, 5 alpha-androstane-3 alpha,17 beta-diol (3 alpha-diol), 5 alpha-androstane-3 beta, 17 beta-diol (3 beta-diol), estrone, and estradiol levels were determined by radioimmunoassay in the different lobules of the testis of Salamandra salamandra throughout...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Lecouteux A,Garnier DH,Bassez T,Joly J

    更新日期:1985-05-01 00:00:00

  • Biological indices, energy reserves, steroid hormones and sexual maturity in the infaunal bivalve Scrobicularia plana from three sites differing by their level of contamination.

    abstract::The aim of this work was to investigate in situ biometric (condition index, hepato-somatic index, gonado-somatic index), biochemical (glycogen, lipids, sexual steroids) and histological (sex, sexual maturation stage) variables in the infaunal bivalve Scrobicularia plana. First, the reproductive cycle of S. plana was a...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Mouneyrac C,Linot S,Amiard JC,Amiard-Triquet C,Métais I,Durou C,Minier C,Pellerin J

    更新日期:2008-06-01 00:00:00

  • Gastroenteropancreatic hormones (insulin, glucagon, somatostatin, and multiple forms of PYY) from the pallid sturgeon, Scaphirhynchus albus (Acipenseriformes).

    abstract::Insulin, glucagon, somatostatin-14, and three structurally related molecular forms of peptide tyrosine-tyrosine (PYY) were isolated from an extract of the combined pancreas and gastrointestinal tract of the pallid sturgeon, Scaphirhynchus albus. Pallid sturgeon insulin was identical to insulin from the Russian sturgeo...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Kim JB,Gadsbøll V,Whittaker J,Barton BA,Conlon JM

    更新日期:2000-12-01 00:00:00

  • Baseline and stress-induced glucocorticoid concentrations are not repeatable but covary within individual great tits (Parus major).

    abstract::In evolutionary endocrinology, there is a growing interest in the extent and basis of individual variation in endocrine traits, especially circulating concentrations of hormones. This is important because if targeted by selection, such individual differences present the opportunity for an evolutionary response to sele...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Baugh AT,van Oers K,Dingemanse NJ,Hau M

    更新日期:2014-11-01 00:00:00

  • Effect of cortisol on the in vitro hepatic conversion of thyroxine to triiodothyronine in brook charr (Salvelinus fontinalis Mitchill).

    abstract::In vitro studies with liver homogenates of brook charr (Salvelinus fontinalis) demonstrate that the conversion of thyroxine (T4) to triiodothyronine (T3) is dependent upon temperature, pH, substrate, and homogenate concentration, supporting the hypothesis of an enzymatic hepatic T4 to T3 conversion. Dithiothreitol has...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Vijayan MM,Flett PA,Leatherland JF

    更新日期:1988-05-01 00:00:00

  • Neurons that co-localize aromatase- and kisspeptin-like immunoreactivity may regulate the HPG axis of the Mallard drake (Anas platyrhynchos).

    abstract::Kisspeptin is a potent regulator of the hypothalamo-pituitary-gonadal axis. The activation of several vernal and pubertal behaviors involves the action of locally synthesized estradiol by hypothalamic aromatase-expressing neurons. Little is known about kisspeptin in non-mammalian systems, and its interaction with arom...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Saldanha CJ,Walters BJ,Fraley GS

    更新日期:2010-05-01 00:00:00

  • Purification, characterization and expression of two vitellogenins in the Indian freshwater murrel Channa punctatus.

    abstract::The present study was undertaken to characterize different vitellogenins in Channa punctatus. Protein purification by gel chromatography followed by fast protein liquid chromatography (FPLC) revealed existence of two different Vg forms. Liquid chromatography tandem mass spectrophotometry (LC-MS/MS) suggested the exist...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Rawat VS,Pipil S,Sharma L,Sehgal N

    更新日期:2013-08-01 00:00:00

  • Melatonin synthesis and clock gene regulation in the pineal organ of teleost fish compared to mammals: Similarities and differences.

    abstract::The pineal organ of all vertebrates synthesizes and secretes melatonin in a rhythmic manner due to the circadian rhythm in the activity of arylalkylamine N-acetyltransferase (AANAT) - the rate-limiting enzyme in melatonin synthesis pathway. Nighttime increase in AANAT activity and melatonin synthesis depends on increa...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章,评审


    authors: Saha S,Singh KM,Gupta BBP

    更新日期:2019-08-01 00:00:00

  • Effects of pimozide and des Gly10,[D-Ala6]luteinizing hormone-releasing hormone ethylamide on serum gonadotropin concentrations, germinal vesicle migration, and ovulation in female goldfish, Carassius auratus.

    abstract::In gravid female goldfish held at 10-12 degrees, a single injection of des Gly10,[D-Ala6]luteinizing hormone-releasing hormone ethylamide (LHRH-A), or 2 injections of LHRH-A given 12 hr apart, increased serum gonadotropin (GtH) concentrations, but did not stimulate germinal vesicle migration or ovulation. Injection of...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Chang JP,Peter RE

    更新日期:1983-10-01 00:00:00

  • Involvement of neuropeptide Y Y1 receptors in the regulation of LH and GH cells in the pituitary of the catfish, Clarias batrachus: an immunocytochemical study.

    abstract::Although neuropeptide Y (NPY) has been known to influence the release of luteinizing hormone (LH) and growth hormone (GH) from the pituitary gland of teleosts, the NPY receptor subtypes involved in the regulatory processes have not been fully defined. An attempt has been made to study the involvement of NPY Y1 recepto...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Mazumdar M,Lal B,Sakharkar AJ,Deshmukh M,Singru PS,Subhedar N

    更新日期:2006-11-01 00:00:00

  • Neuropeptide Evolution: Chelicerate Neurohormone and Neuropeptide Genes may reflect one or more whole genome duplications.

    abstract::Four genomes and two transcriptomes from six Chelicerate species were analyzed for the presence of neuropeptide and neurohormone precursors and their GPCRs. The genome from the spider Stegodyphus mimosarum yielded 87 neuropeptide precursors and 101 neuropeptide GPCRs. High neuropeptide transcripts were also found in t...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Veenstra JA

    更新日期:2016-11-09 00:00:00

  • Modulation of the prolactin response to thyrotropin releasing hormone by ovarian steroids in ovariectomized turkeys (Meleagris gallopavo).

    abstract::The effect of thyrotropin releasing hormone (TRH) administered intramuscularly (im) on serum levels of prolactin (Prl) in ovariectomized (ovx) adult turkeys before and following the onset of photostimulation, before and during daily administration (im) of progesterone (P; 0.1, 0.4, or 1.0 mg/kg), estradiol benzoate (E...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Saeed W,el Halawani ME

    更新日期:1986-04-01 00:00:00

  • Relative roles of theca and granulosa cells in ovarian follicular steroidogenesis in the amphibian, Rana nigromaculata.

    abstract::Using frog ovarian follicles in vitro we assessed the role of the theca/epithelium (THEP) layer and granulosa cells in the production of steroids. The THEP layer and granulosa cell-enclosed oocytes (GCEOs) were separated from ovarian follicles of the frog, Rana nigromaculata, by microdissection. Intact follicles (IFs)...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Kwon HB,Ahn RS

    更新日期:1994-05-01 00:00:00

  • Hormone studies in Myxine glutinosa: failure of calcitonin to influence urine flow or plasma and urine electrolyte balance.

    abstract::Hagfish, Myxine glutinosa, were used in a series of experiments to determine a possible role for calcitonin in plasma and urine electrolyte balance. Individual animals were anaesthetised and implanted with polythene cannulae for monitoring blood pressure and for infusion/injection. Urine was collected by manual palpat...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Wales NA

    更新日期:1988-07-01 00:00:00

  • Abnormal growth hormone receptor gene expression in the sex-linked dwarf chicken.

    abstract::Sex-linked dwarfism is a recessive mutation that causes a reduction in body weight gain and long bone growth of chickens. We examined the effect of the dwarfing gene on body weight, hepatic GH-binding activity, and the structure and expression of the growth hormone receptor (GHR) gene in two different lines of sex-lin...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Burnside J,Liou SS,Zhong C,Cogburn LA

    更新日期:1992-10-01 00:00:00

  • Production of symmetrical flatfish by controlling the timing of thyroid hormone treatment in spotted halibut Verasper variegatus.

    abstract::The thyroid hormone plays an essential role in the metamorphosis in flatfish, during which external asymmetry (for example, eye relocation and pigmentation) is established. However, no information is available on the expression mechanisms of metamorphic asymmetry. Since the presence of malformed juveniles (having eith...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Tagawa M,Aritaki M

    更新日期:2005-04-01 00:00:00

  • Osmoregulatory effects of prolactin and growth hormone in embryonic chicks.

    abstract::Intact White Leghorn chick embryos were treated daily (on Days 6-13) with bovine prolactin (PRL) or ovine growth hormone (GH) at doses of 4-10 micrograms/g embryo wet wt. A control group received an equal volume of avian saline. [Na+] and [Cl-] were determined in allantoic fluid samples taken on Days 10, 12, and 14, a...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Murphy MJ,Brown PS,Brown SC

    更新日期:1986-06-01 00:00:00

  • Sexually attractive phrases increase yolk androgens deposition in Canaries (Serinus canaria).

    abstract::The androgen concentration in birds' eggs varies with laying order, breeding conditions, and mate attractiveness. In passerine birds, mate attractiveness depends upon song quality. The aim of our study was to evaluate the effect of one criterion used by females to assess male song quality that is to say the presence o...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Tanvez A,Béguin N,Chastel O,Lacroix A,Leboucher G

    更新日期:2004-09-01 00:00:00

  • Peptidergic innervation of the adrenocorticotropic hormone (ACTH)- and growth hormone (GH)-producing cells in the pars distalis of the sea bass (Dicentrarchus labrax).

    abstract::Due to its unique organization, the teleost pituitary is an ideal model in which to investigate the relationship of the nervous system with the pituitary endocrine cells. A light microscope immunocytochemical study of the sea bass pituitary revealed six different neuropeptides in nerve fibers which projected into the ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Moons L,Cambré M,Marivoet S,Batten TF,Vanderhaeghen JJ,Ollevier F,Vandesande F

    更新日期:1988-11-01 00:00:00

  • Salt intake and plasma renin activity in the freshwater turtle.

    abstract::In mammals, a well-documented inverse relationship exists between oral salt intake and plasma renin activity (PRA). We carried out this study to determine if a similar relationship exists in the freshwater turtle Pseudemys scripta. Three groups of turtles (N = 8 for each group) were fed different amounts of salt disso...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Stephens GA,Hauben D

    更新日期:1989-12-01 00:00:00

  • Within-sample variation of fecal glucocorticoid measurements.

    abstract::Fecal glucocorticoid metabolite analysis is a useful tool for monitoring adrenocortical activity in captive and free-ranging wildlife. Glucocorticoid metabolites may not be evenly distributed within fecal samples and this variability could affect the interpretation of glucocorticoid metabolite measurements. Furthermor...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Millspaugh JJ,Washburn BE

    更新日期:2003-06-01 00:00:00

  • Hypothalamo-adenohypophyseal-thyroid interrelationships in the chick embryo. VI. Midgestational adenohypophyseal sensitivity to thyrotrophin-releasing hormone.

    abstract::The functional abilities of 9.5-, 10.5-, 11.5-, and 12.5-day-old chick embryos to respond to exogenous thyrotrophin-releasing hormone (TRH) were evaluated by means of changes in plasma total thyroxine (T4). T4 concentrations were determined 3.0, 6.0, 12.0, and 24.0 hr after TRH treatment. The data of the present inves...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Thommes RC,Williams DJ,Woods JE

    更新日期:1984-08-01 00:00:00

  • Isolation and biological characterization of a 6-kDa protein from hepatopancreas of lobster Panulirus argus with insulin-like effects.

    abstract::A protein with insulin-like effects was isolated from the hepatopancreas of the lobster Panulirus argus following a classic method for mammalian insulin purification from the pancreas. After acid-alcoholic extraction and ethanol-ether precipitation followed by molecular filtration chromatography, a protein with an app...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Gallardo N,Carrillo O,Moltó E,Deás M,González-Suárez R,Carrascosa JM,Ros M,Andrés A

    更新日期:2003-05-01 00:00:00

  • The effects of estrogen on skeletal calcium metabolism and on plasma parameters of vitellogenesis in the male, three-toed box turtle (Terrapene carolina triunguis).

    abstract::The box turtle (Terrapene carolina triunguis) does not appear to have evolved a mechanism for secondary bone formation similar to Aves inasmuch as several parameters of bone calcium metabolism were unaffected by estrogen treatment. This suggests that reproduction does not constitute a substantial stress to the calcium...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Magliola L

    更新日期:1984-04-01 00:00:00

  • A nonsteroidal follicular factor is involved in maturation process of Xenopus laevis oocytes.

    abstract::Xenopus laevis postvitellogenic ovarian follicles release into conditioned medium nonsteroidal factor(s) that potentiate progesterone-induced oocyte maturation. The molecular weight of this factor is between 1000 and 10,000 Da. The potentiating activity is suppressed when follicles are incubated with cyanoketone to in...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Chesnel F,Bourry A,Boujard D,Joly J

    更新日期:1992-05-01 00:00:00