The complexity of nitrosoguanidine mutagenesis increases with size: observations of the mutational specificity of N-propyl-N'-nitro-N-nitrosoguanidine.


:The mutational specificity of the monofunctional alkylating agent N-propyl-N'-nitro-N-nitrosoguanidine (PNNG) has been determined through the DNA sequence characterization of 109 LacI- mutations of Escherichia coli. The predominant mutation induced was the G:C----A:T transition (73%), presumably the result of O6-propylguanine damage. Transversions constituted 18% of the mutants, almost entirely due to G:C----T:A (9%) and A:T----C:G (8%) events. Two identical deletions, one single base pair frameshift and a tandem double base change were also recovered. In contrast, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was previously found to induce only transitions, again predominantly G:C----A:T events (98%). Moreover, the site specificity observed for PNNG-induced G:C----A:T transitions is quite distinct from that induced by MNNG. G:C----A:T transitions recovered following PNNG treatment do not appear to be influenced by neighbouring base sequence to the extent seen for MNNG.






van der Vliet GM,Zielenska M,Anderson MW,Glickman BW




Has Abstract


1989-05-01 00:00:00












  • Membrane-type matrix metalloproteinases mediate curcumin-induced cell migration in non-tumorigenic colon epithelial cells differing in Apc genotype.

    abstract::Colonic epithelial cell migration is required for normal differentiated cell function. This migratory phenotype is dependent upon wild-type adenomatous polyposis coli (Apc) expression. Non-tumorigenic murine colon epithelial cell lines with distinct Apc genotypes, i.e. young adult mouse colon (YAMC; Apc(+/+)) and immo...


    pub_type: 杂志文章


    authors: Fenton JI,Wolff MS,Orth MW,Hord NG

    更新日期:2002-06-01 00:00:00

  • Read-through transcripts in lung: germline genetic regulation and correlation with the expression of other genes.

    abstract::Transcripts originating from the transcriptional read through of two adjacent, similarly oriented genes have been identified in normal and neoplastic tissues, but their functional role and the mechanisms that regulate their expression are mostly unknown. Here, we investigated whether the expression of read-through tra...


    pub_type: 杂志文章


    authors: Maspero D,Dassano A,Pintarelli G,Noci S,De Cecco L,Incarbone M,Tosi D,Santambrogio L,Dragani TA,Colombo F

    更新日期:2020-07-14 00:00:00

  • Estrogen-responsive RING finger mRNA induction in gastrointestinal carcinoma cells following bile acid treatment.

    abstract::The underlying molecular mechanisms of the tumor-promoting activity of bile acids such as chenodeoxycholic acid (CDCA) and deoxycholic acid (DCA) and the protective effect of ursodeoxycholic acid (UDCA) remain largely unclear. Using RNA arbitrarily primed PCR (RAP-PCR) for differential display, we identified, cloned a...


    pub_type: 杂志文章


    authors: Jung B,Vogt T,Mathieu-Daudé F,Welsh J,McClelland M,Trenkle T,Weitzel C,Kullmann F

    更新日期:1998-11-01 00:00:00

  • Caffeic acid causes metal-dependent damage to cellular and isolated DNA through H2O2 formation.

    abstract::Pulsed field gel electrophoresis showed that caffeic acid induced DNA strand breaks in cultured human cells in the presence of Mn(II). With alkali treatment, DNA single-strand breaks were observed. The strand breakage was increased by the treatment of buthionine sulphoximine (a GSH synthesis inhibitor) and 3-aminotria...


    pub_type: 杂志文章


    authors: Inoue S,Ito K,Yamamoto K,Kawanishi S

    更新日期:1992-09-01 00:00:00

  • Retinoic acid and 4-hydroxyphenylretinamide induce growth inhibition and tissue transglutaminase through different signal transduction pathways in mouse fibroblasts (NIH 3T3 cells).

    abstract::4-Hydroxyphenylretinamide (4-HPR) is a synthetic retinoid with minimal toxicity and favorable pharmacokinetics during long-term administration to patients in clinical trials. Since 4-HPR binds poorly to the retinoic acid receptors, the issue of whether 4-HPR exerts its biological actions via classical retinoid recepto...


    pub_type: 杂志文章


    authors: Giandomenico V,Andreola F,Rodriguez de la Concepcion ML,Collins SJ,De Luca LM

    更新日期:1999-06-01 00:00:00

  • Elevation of serum phase II enzymes by anticarcinogenic enzyme inducers: markers for a chemoprotected state?

    abstract::Inducers of Phase II enzymes, already consumed by humans as food additives, medicines or as constituents of vegetables, can prevent experimental carcinogenesis. Since protection is neither carcinogen- nor organ-specific, clinical trials are already underway to establish the efficacy of 'anticarcinogenic enzyme inducer...


    pub_type: 杂志文章


    authors: Prochaska HJ,Fernandes CL

    更新日期:1993-12-01 00:00:00

  • Sulindac reversal of 15-PGDH-mediated resistance to colon tumor chemoprevention with NSAIDs.

    abstract::Non-steroidal anti-inflammatory drugs prevent colorectal cancer by inhibiting cyclooxygenase (COX) enzymes that synthesize tumor-promoting prostaglandins. 15-hydroxyprostaglandin dehydrogenase (15-PGDH) is a tumor suppressor that degrades tumor-promoting prostaglandins. Murine knockout of 15-PGDH increases susceptibil...


    pub_type: 杂志文章


    authors: Fink SP,Dawson DM,Zhang Y,Kresak A,Lawrence EG,Yang P,Chen Y,Barnholtz-Sloan JS,Willis JE,Kopelovich L,Markowitz SD

    更新日期:2015-02-01 00:00:00

  • A variant in the CHEK2 promoter at a methylation site relieves transcriptional repression and confers reduced risk of lung cancer.

    abstract::Checkpoint kinase (CHEK) 2, a tumor suppressor gene, plays an essential role in the DNA damage checkpoint response cascade. We first investigated two polymorphisms in the proximal promoter of the CHEK2 gene and evaluated their associations with the risk of lung cancer in a case-control study using 500 incident lung ca...


    pub_type: 杂志文章


    authors: Zhang S,Lu J,Zhao X,Wu W,Wang H,Lu J,Wu Q,Chen X,Fan W,Chen H,Wang F,Hu Z,Jin L,Wei Q,Shen H,Huang W,Lu D

    更新日期:2010-07-01 00:00:00

  • Identification and quantitative detection of isomeric benzo[a]pyrene diolepoxide--DNA adducts by low-temperature conventional fluorescence methods.

    abstract::The pyrene-like fluorescence of adducts derived from the covalent binding of (+/-)-trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9, 10-tetrahydrobenzo[a]pyrene [+/-]-anti-BPDE] to DNA increases in intensity by factors of 20 or more as the temperature is lowered from ambient to approximately 100 K. This effect is primarily a...


    pub_type: 杂志文章


    authors: Zhao R,Liu TM,Kim SK,MacLeod MC,Geacintov NE

    更新日期:1992-10-01 00:00:00

  • EGF promotes neuroendocrine-like differentiation of prostate cancer cells in the presence of LY294002 through increased ErbB2 expression independent of the phosphatidylinositol 3-kinase-AKT pathway.

    abstract::An increased neuroendocrine (NE) cell population in prostate cancer is associated with more aggressive disease and recurrence after androgen-deprivation therapy, although the mechanism responsible is unknown. In this study, we report that the treatment of LNCaP cells with epidermal growth factor (EGF) in the presence ...


    pub_type: 杂志文章


    authors: Cortés MA,Cariaga-Martinez AE,Lobo MV,Martín Orozco RM,Motiño O,Rodríguez-Ubreva FJ,Angulo J,López-Ruiz P,Colás B

    更新日期:2012-06-01 00:00:00

  • 3-Methylcholanthrene-inhibition of hepatocarcinogenesis in the rat due to 3'-methyl-4-dimethylaminoazobenzene or 2-acetylamino-fluorene: a comparative study.

    abstract::Male Leeds strain rats were given a diet containing either 0.06% 3'-methyl-4-dimethylaminoazobenzene (3'-MeDAB) or 0.05% 2-acetylaminofluorene (2-AAF), alone or together with 0.0067% 3-methylcholanthrene (MeCh). MeCh both reduced the incidence of hepatic neoplasms induced by 3'-MeDAB and delayed their development. Wit...


    pub_type: 杂志文章


    authors: Flaks A,Flaks B

    更新日期:1982-01-01 00:00:00

  • p53 and Ha-ras mutations in chemically induced hamster buccal pouch carcinomas.

    abstract::Well-differentiated squamous cell carcinomas were induced in hamster buccal pouch epithelium by twice weekly topical applications of N-methyl-N-benzylnitrosamine (MBN) or 7,12-dimethylbenz[a]anthracene (DMBA) over a period of 15 weeks. Each of the 22 tumors induced (14 MBN and eight DMBA) were evaluated by single-stra...


    pub_type: 杂志文章


    authors: Chang KW,Lin SC,Koos S,Pather K,Solt D

    更新日期:1996-03-01 00:00:00

  • PDGF AA as mediator in nicotine-dependent carcinogenesis.

    abstract::Effect of nicotine on PDGF AA and PDGF BB interaction with cervical cancer SiHa cells was tested. [125I]PDGF AA was internalized by cells and accumulated in the cytoplasm and nucleus (chromatin). In the absence of nicotine, maximal accumulation of [125I]PDGF AA inside the cells occurred after 1 day of incubation, whic...


    pub_type: 杂志文章


    authors: Rakowicz-Szulczynska EM,McIntosh DG,Perry M,Smith ML

    更新日期:1996-09-01 00:00:00

  • Possible anti-tumour-promoting activity of components in Japanese soybean fermented food, Natto: effect on gap junctional intercellular communication.

    abstract::In order to detect any protective agent against tumor formation, we examined the anti-tumor-promoting effect of a Japanese traditional soybean fermented food, Natto. Dye transfer was employed as an assay method. When fluorescent dye was microinjected into cultured BALB/3T3 cells, the dye was transformed into the neigh...


    pub_type: 杂志文章


    authors: Takahashi C,Kikuchi N,Katou N,Miki T,Yanagida F,Umeda M

    更新日期:1995-03-01 00:00:00

  • The association of microsomal epoxide hydrolase polymorphisms and lung cancer risk in African-Americans and Mexican-Americans.

    abstract::This study evaluated the influence of genetic polymorphisms in the microsomal epoxide hydrolase (mEPHX) gene on lung cancer risk in a case-control study of two different ethnic groups, Mexican-Americans and African-Americans. There were 138 lung cancer cases (60 Mexican-American and 78 African-American) and 148 contro...


    pub_type: 杂志文章


    authors: Wu X,Gwyn K,Amos CI,Makan N,Hong WK,Spitz MR

    更新日期:2001-06-01 00:00:00

  • Induction of free radicals and tumors in the kidneys of Wistar rats by ferric ethylenediamine-N,N'-diacetate.

    abstract::An iron chelate, ferric ethylenediamine-N,N'-diacetate [Fe(III)-EDDA], was found to produce hydroxyl radicals with hydrogen peroxide, as determined by both a deoxyribose degradation test and electron spin resonance. Hydroxyl radical production was inhibited not only by adding hydroxyl radical scavengers and catalase, ...


    pub_type: 杂志文章


    authors: Liu M,Okada S

    更新日期:1994-12-01 00:00:00

  • Mutagenicity of the mononitrobenzo[a]pyrenes in Chinese hamster ovary cells mediated by rat hepatocytes or liver S9.

    abstract::Previous studies have shown that 1- and 3-nitrobenzo[a]pyrene (NBaP) were mutagenic in the Salmonella reversion assay without exogenous activation and that 1-, 3- and 6-NBaP were mutagenic in the presence of hepatocytes or liver homogenate (S9). In the present study, 1-, 3- and 6-NBaP were tested for mutagenicity in C...


    pub_type: 杂志文章


    authors: Hass BS,Heflich RH,Schol HM,Chou MW,Fu PP,Casciano DA

    更新日期:1986-04-01 00:00:00

  • The effect of the format of administration and the total dose of phenobarbital on altered hepatic foci following initiation in female rats with diethylnitrosamine.

    abstract::The effect of changing the format of administration as well as the total dose of the promoting agent phenobarbital (PB) on the development of altered hepatic foci (AHF) was determined in an initiation-promotion protocol with female rats fed the purified AIN-76 diet. Effects on the total number of AHF and the volume pe...


    pub_type: 杂志文章


    authors: Xu YD,Dragan YP,Young T,Pitot HC

    更新日期:1991-06-01 00:00:00

  • Oligonucleotide microarray analysis of gene expression in neuroblastoma displaying loss of chromosome 11q.

    abstract::A number of distinct subtypes of neuroblastoma exist with different genetic abnormalities that are predicative of outcome. Whole chromosome gains are usually associated with low stage disease and favourable outcome, whereas loss of 1p, 3p and 11q, unbalanced gain of 17q and MYCN amplification (MNA) are indicative of h...


    pub_type: 杂志文章,多中心研究,评审


    authors: McArdle L,McDermott M,Purcell R,Grehan D,O'Meara A,Breatnach F,Catchpoole D,Culhane AC,Jeffery I,Gallagher WM,Stallings RL

    更新日期:2004-09-01 00:00:00

  • Diffusion of dialkylnitrosamines into the rat esophagus as a factor in esophageal carcinogenesis.

    abstract::To indicate how readily nitrosamines (NAms) diffuse into the esophagus, we measured diffusion rate (flux) through rat esophagus of dialkyl-NAms using side-by-side diffusion apparatuses. Mucosal and serosal flux at 37 degrees C of two NAms, each at 50 microM, was followed for 90 min by gas chromatography-thermal energy...


    pub_type: 杂志文章


    authors: Haorah J,Miller DW,Brand R,Smyrk TC,Wang X,Chen SC,Mirvish SS

    更新日期:1999-05-01 00:00:00

  • Urolithin A gains in antiproliferative capacity by reducing the glycolytic potential via the p53/TIGAR axis in colon cancer cells.

    abstract::Polyphenols have shown promising bioactivity in experimental in vitro and in vivo models for cancer chemoprevention. However, consumed orally, they are often transformed by gut microbes into new active principles with so far incompletely deciphered molecular mechanisms. Here, enterolacton, S-equol and urolithin A as r...


    pub_type: 杂志文章


    authors: Norden E,Heiss EH

    更新日期:2019-03-12 00:00:00

  • Differential susceptibility of a fish, tilapia Oreochromis mossambicus (Teleostei, Cichlidae) to hepatocarcinogenesis by diethylnitrosamine and methylazoxymethanol acetate.

    abstract::Oreochromis mossambicus, commonly known as tilapia, is a freshwater teleost with a wide tolerance to environmental conditions. Multiple focal lesions in the liver were observed 2 months after cessation of a one-month long treatment with 100 p.p.m. diethylnitrosamine. Cells were small and compact and arranged in sheets...


    pub_type: 杂志文章


    authors: Ding JL,Hee PL,Lam TJ

    更新日期:1989-03-01 00:00:00

  • Identification of benzo[a]pyrene-7,8-dione as an authentic metabolite of (+/-)-trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene in isolated rat hepatocytes.

    abstract::Dihydrodiol dehydrogenase (DD) has been shown to catalyze the oxidation of (+/-)-trans-7,8-dihydroxy-7,8-dihydrobenzo [a]pyrene (BP-diol) to yield benzo[a]pyrene-7,8-dione (BPQ) in uninduced fortified rat liver S100 fractions but the formation of BPQ has not been observed in whole cells. In these studies [3H]BP-diol w...


    pub_type: 杂志文章


    authors: Flowers-Geary L,Harvey RG,Penning TM

    更新日期:1995-11-01 00:00:00

  • Induction of the aromatic hydrocarbon receptor by trans-4-acetylaminostilbene in rat liver. Comparison with other aromatic amines.

    abstract::The effect of the aromatic amines 2-acetylaminofluorene (AAF), 2-acetylaminophenanthrene (AAP) and trans-4-acetylaminostilbene (AAS) on the rat hepatic aromatic hydrocarbon (Ah) receptor level was studied. 3-Methylcholanthrene (MC), as a known receptor ligand, was used as a control. The complete liver carcinogen AAF a...


    pub_type: 杂志文章


    authors: Göttlicher M,Cikryt P

    更新日期:1987-07-01 00:00:00

  • CYP1A1 and GSTM1 genotypes affect benzo[a]pyrene DNA adducts in smokers' lung: comparison with aromatic/hydrophobic adduct formation.

    abstract::Benzo[a]pyrene diol epoxide (BPDE)-DNA adducts are involved in the induction of p53 mutations and probably in the causation of human lung cancer associated with cigarette smoking. The ratio between CYP1A1 and GST enzyme activities is a critical determinant of the target dose of carcinogenic BPDE and other DNA-reactive...


    pub_type: 杂志文章,评审


    authors: Alexandrov K,Cascorbi I,Rojas M,Bouvier G,Kriek E,Bartsch H

    更新日期:2002-12-01 00:00:00

  • The influence of inhibitors on the repair of benzo[a]pyrene-damaged DNA in hamster tracheal epithelial cells.

    abstract::We investigated, in a cloned hamster tracheal epithelial cell line HTE-B, the effects of inhibitors of DNA topoisomerase, novobiocin and nalidixic acid; of DNA polymerase, 1-beta-arabinofuranosylcytosine (ara-C) and 2',3'-dideoxythymidine; of ribonucleotide reductase, hydroxyurea; and of poly(ADP-ribose)synthetase, 3-...


    pub_type: 杂志文章


    authors: Ireland CM,Eastman A,Bresnick E

    更新日期:1984-02-01 00:00:00

  • Allyl mercaptan, a garlic-derived organosulfur compound, inhibits histone deacetylase and enhances Sp3 binding on the P21WAF1 promoter.

    abstract::Histone deacetylase (HDAC) inhibitors have the potential to derepress epigenetically silenced genes in cancer cells, leading to cell cycle arrest and apoptosis. In the present study, we screened several garlic-derived small organosulfur compounds for their ability to inhibit HDAC activity in vitro. Among the organosul...


    pub_type: 杂志文章


    authors: Nian H,Delage B,Pinto JT,Dashwood RH

    更新日期:2008-09-01 00:00:00

  • Osteopontin is a downstream effector of the PI3-kinase pathway in melanomas that is inversely correlated with functional PTEN.

    abstract::The tumor suppressor PTEN antagonizes phosphatidylinositol 3-kinase (PI3K), which contributes to tumorigenesis in many cancer types. While PTEN mutations occur in some melanomas, their precise mechanistic consequences have yet to be elucidated. We sought to identify novel downstream effectors of PI3K using a combinati...


    pub_type: 杂志文章


    authors: Packer L,Pavey S,Parker A,Stark M,Johansson P,Clarke B,Pollock P,Ringner M,Hayward N

    更新日期:2006-09-01 00:00:00

  • Occupational exposure to heavy metals: DNA damage induction and DNA repair inhibition prove co-exposures to cadmium, cobalt and lead as more dangerous than hitherto expected.

    abstract::Co-exposure to cadmium, cobalt, lead and other heavy metals occurs in many occupational settings, such as pigment and batteries production, galvanization and recycling of electric tools. However, little is known about interactions between several heavy metals. In the present study we determined DNA single strand break...


    pub_type: 杂志文章


    authors: Hengstler JG,Bolm-Audorff U,Faldum A,Janssen K,Reifenrath M,Götte W,Jung D,Mayer-Popken O,Fuchs J,Gebhard S,Bienfait HG,Schlink K,Dietrich C,Faust D,Epe B,Oesch F

    更新日期:2003-01-01 00:00:00

  • Etheno adducts in spleen DNA of SJL mice stimulated to overproduce nitric oxide.

    abstract::In order to investigate specific DNA damage caused by nitric oxide (NO) induced lipid peroxidation, levels of promutagenic etheno adducts 1,N6-ethenodeoxyadenosine (epsilondA) and 3,N4-ethenodeoxycytidine (epsilondC) were measured in spleen DNA of SJL mice induced to produce high levels of NO by injection of RcsX (pre...


    pub_type: 杂志文章


    authors: Nair J,Gal A,Tamir S,Tannenbaum SR,Wogan GN,Bartsch H

    更新日期:1998-12-01 00:00:00