The complexity of nitrosoguanidine mutagenesis increases with size: observations of the mutational specificity of N-propyl-N'-nitro-N-nitrosoguanidine.


:The mutational specificity of the monofunctional alkylating agent N-propyl-N'-nitro-N-nitrosoguanidine (PNNG) has been determined through the DNA sequence characterization of 109 LacI- mutations of Escherichia coli. The predominant mutation induced was the G:C----A:T transition (73%), presumably the result of O6-propylguanine damage. Transversions constituted 18% of the mutants, almost entirely due to G:C----T:A (9%) and A:T----C:G (8%) events. Two identical deletions, one single base pair frameshift and a tandem double base change were also recovered. In contrast, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was previously found to induce only transitions, again predominantly G:C----A:T events (98%). Moreover, the site specificity observed for PNNG-induced G:C----A:T transitions is quite distinct from that induced by MNNG. G:C----A:T transitions recovered following PNNG treatment do not appear to be influenced by neighbouring base sequence to the extent seen for MNNG.






van der Vliet GM,Zielenska M,Anderson MW,Glickman BW




Has Abstract


1989-05-01 00:00:00












  • Diallyl disulfide inhibits p34(cdc2) kinase activity through changes in complex formation and phosphorylation.

    abstract::Previous studies from our laboratory demonstrated that diallyl disulfide (DADS), an oil-soluble allyl sulfur compound found in processed garlic, markedly suppressed p34(cdc2) kinase activity and induced a G(2)/M phase arrest in cultured human colon tumor (HCT-15) cells. The present studies reveal that suppression of p...


    pub_type: 杂志文章


    authors: Knowles LM,Milner JA

    更新日期:2000-06-01 00:00:00

  • Biomonitoring an albumin adduct of the cooked meat carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in humans.

    abstract::2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is formed in cooked meats and may be linked to dietary-associated colorectal, prostate and mammary cancers. Genotoxic N-oxidized metabolites of PhIP react with the Cys34 of albumin (Alb) to form a sulfinamide adduct, a biomarker of the biologically effective dose....


    pub_type: 杂志文章


    authors: Bellamri M,Wang Y,Yonemori K,White KK,Wilkens LR,Le Marchand L,Turesky RJ

    更新日期:2018-12-31 00:00:00

  • The metastasis suppressor, NDRG1, attenuates oncogenic TGF-β and NF-κB signaling to enhance membrane E-cadherin expression in pancreatic cancer cells.

    abstract::The metastasis suppressor, N-myc downstream-regulated gene-1 (NDRG1), plays multifaceted roles in inhibiting oncogenic signaling and can suppress the epithelial mesenchymal transition (EMT), a key step in metastasis. In this investigation, NDRG1 inhibited the oncogenic effects of transforming growth factor-β (TGF-β) i...


    pub_type: 杂志文章


    authors: Menezes SV,Fouani L,Huang MLH,Geleta B,Maleki S,Richardson A,Richardson DR,Kovacevic Z

    更新日期:2019-07-06 00:00:00

  • Decreased lung tumorigenesis in mice genetically deficient in cytosolic phospholipase A2.

    abstract::Epidemiological investigations suggest that chronic lung inflammation increases lung cancer risk. Pharmacologic and genetic evidence in mouse models indicates that lipid mediators released during pulmonary inflammation enhance lung tumor formation. Cytosolic phospholipase A2 (cPLA2) catalyzes arachidonic acid (AA) rel...


    pub_type: 杂志文章


    authors: Meyer AM,Dwyer-Nield LD,Hurteau GJ,Keith RL,O'Leary E,You M,Bonventre JV,Nemenoff RA,Malkinson AM

    更新日期:2004-08-01 00:00:00

  • Carcinogenicity of 3'-hydroxymethyl-N,N-dimethyl-4-aminoazobenzene in rat liver.

    abstract::Male Sprague-Dawley rats were fed a cube diet containing 2.51 mmol/kg 3'-hydroxymethyl-N,N-dimethyl-4-aminoazobenzene (3'-CH2OH-DAB) for a period of 1 or 3 months. (This is the molar equivalent of 0.06% 3'-Me-DAB in the diet). The oral administration of 3'-CH2OH-DAB for 3 months resulted in a high incidence of liver t...


    pub_type: 杂志文章


    authors: Mori Y,Niwa T,Toyoshi K,Nagai H,Koda A,Kawada K,Ojima A,Takahashi Y

    更新日期:1980-06-01 00:00:00

  • Nafenopin-induced rat liver peroxisome proliferation reduces DNA methylation by N-nitrosodimethylamine in vivo.

    abstract::The hypolipidaemic drug nafenopin (NAF) has been shown to enhance the hepatocarcinogenic effect of N-nitrosodimethylamine (NDMA) and N-nitrosodiethylamine in rats. We have investigated whether the NAF-induced peroxisome proliferation in hepatocytes interferes with NDMA's metabolism and interaction with DNA. Adult male...


    pub_type: 杂志文章


    authors: Wiestler OD,Schmerold I,Fringes B,Volk B,Kleihues P

    更新日期:1985-09-01 00:00:00

  • Rapid induction of colon carcinogenesis in CYP1A-humanized mice by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and dextran sodium sulfate.

    abstract::2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), the most abundant heterocyclic amine produced during the cooking of meats and fish, is suspected to be a human carcinogen. Metabolic activation of PhIP is primarily mediated by the enzyme cytochrome P450 (CYP) 1A2. Metabolism of PhIP by CYP1A2 differs considerabl...


    pub_type: 杂志文章


    authors: Cheung C,Loy S,Li GX,Liu AB,Yang CS

    更新日期:2011-02-01 00:00:00

  • Identification of two keratinocyte stem cell regulatory loci implicated in skin carcinogenesis.

    abstract::Several converging lines of evidence suggest that keratinocyte stem cells including those clonogenic in vitro, are the target cells in skin carcinogenesis. However, the mechanism of their involvement in carcinogenesis continues to be unknown. Recently, we have demonstrated that the number of keratinocyte clonogenic st...


    pub_type: 杂志文章


    authors: Popova NV,Teti KA,Wu KQ,Morris RJ

    更新日期:2003-03-01 00:00:00

  • 32P-postlabeling analysis of liver DNA adducts in rats chronically fed a choline-devoid diet.

    abstract::Liver DNA, obtained at various time intervals from rats chronically fed a choline-devoid diet, was analysed for the presence of aromatic or alkyl adducts by the 32P-postlabeling assay. Alkyl adducts were not detected. Aromatic DNA adduct lesions were revealed, but only at levels (1 adduct per 0.5-3 X 10(9) nucleotides...


    pub_type: 杂志文章


    authors: Gupta RC,Earley K,Locker J,Lombardi B

    更新日期:1987-01-01 00:00:00

  • Role for EPS8 in squamous carcinogenesis.

    abstract::We have investigated the role of the signaling intermediate, EPS8, in tumor progression using a model system and in vivo. HN4 primary tumor cells express low levels of EPS8, similar to normal keratinocytes, and show minimal invasion in vitro in response to epidermal growth factor, whereas HN12 cells express high level...


    pub_type: 杂志文章


    authors: Wang H,Patel V,Miyazaki H,Gutkind JS,Yeudall WA

    更新日期:2009-01-01 00:00:00

  • Correlation between morphology and blood group-related antigen expression in pancreatic tumors induced in Syrian hamsters.

    abstract::Expression of blood group-related antigens (BGRAs) A, B, H, Le(b), Le(x) and Le(y) was examined immunohistochemically in neoplastic and hyperplastic lesions induced by N-nitrosobis(2-oxopropyl)amine in Syrian hamsters to determine a relationship between histological and biological patterns of the lesion and expression...


    pub_type: 杂志文章


    authors: Tomioka T,Fujii H,Egami H,Takiyama Y,Pour PM

    更新日期:1991-03-01 00:00:00

  • Effect of hexachlorocyclohexane on diethylnitrosamine-induced hepatocarcinogenesis in rat and its failure to promote skin tumors on dimethylbenz[a]anthracene initiation in mouse.

    abstract::The promoting action of hexachlorocyclohexane (HCH) was evaluated in female Wistar rats by counting the gamma-glutamyltranspeptidase-positive foci which appeared in the liver after diethylnitrosamine (DENA) initiation. Liver tumor promotion by HCH was further confirmed in a long-term tumorigenicity study after DENA pr...


    pub_type: 杂志文章


    authors: Munir KM,Rao KV,Bhide SV

    更新日期:1984-04-01 00:00:00

  • Nitric oxide induces cyclooxygenase expression and inhibits cell growth in colon cancer cell lines.

    abstract::The role of nitric oxide (NO) in colon cancer remains controversial. Inducible nitric oxide synthase (iNOS) has been reported to be up regulated and down regulated in colorectal cancer in both animal models and patient tissue samples. Cyclooxygenase-2 (COX-2) is important in colorectal carcinogenesis but its relations...


    pub_type: 杂志文章


    authors: Liu Q,Chan ST,Mahendran R

    更新日期:2003-04-01 00:00:00

  • Evidence that N-acetoxy-N-acetyl-2-aminofluorene crosslinks DNA to protein by a free radical mechanism.

    abstract::A method was devised to quantitate N-acetoxy-N-acetyl-2-aminofluorene (N-AcO-AAF) induced crosslinking of the ionic complex between [methyl-3H]thymidine labeled pBR322 DNA and lysozyme. This involved chromatography of the modified complex on Sephadex CM 25 with a high salt buffer that dissociated the non-covalently bo...


    pub_type: 杂志文章


    authors: Rayshell M,Ross J,Werbin H

    更新日期:1983-01-01 00:00:00

  • Potentiating effect of benzo[a]pyrene and caffeine on Friend viral leukemogenesis.

    abstract::The effect of exposing mice to both a chemical carcinogen and leukemia virus with and without an inhibitor of DNA repair were compared. The data indicated that benzo[a]pyrene (BP) could exert a potentiating effect of Friend viral leukemogenesis in mice, which was dependent on the relative times of administration of th...


    pub_type: 杂志文章


    authors: Raikow RB,Okunewick JP,Buffo MJ,Jones DL,Brozovich BJ,Seeman PR,Koval TM

    更新日期:1981-01-01 00:00:00

  • Cell specificity of rat cytokeratin p39 during azo dye-induced hepatocarcinogenesis.

    abstract::Monoclonal or affinity-purified antibodies specific to Novikoff hepatoma cytokeratin p39 were employed to study the origin and fate of p39-containing cell types during hepatocarcinogenesis induced with N,N-dimethyl-p(m-tolylazo)aniline. Frozen sections were obtained from the livers of animals autopsied temporally duri...


    pub_type: 杂志文章


    authors: Schmidt WN,Page DL,McKusick K,Hnilica LS

    更新日期:1985-08-01 00:00:00

  • Inhibition of intercellular communication in human keratinocytes by fractionated asphalt fume condensates.

    abstract::Asphalt fume condensate (AFC) and chromatographically separated fractions have been shown to cause cancer in mouse skin. The levels of known carcinogenic initiators in these complex mixtures, however, are considered too low to account for their carcinogenic potency. It has been proposed that AFC may contain co-carcino...


    pub_type: 杂志文章


    authors: Wey HE,Breitenstein MJ,Toraason MA

    更新日期:1992-06-01 00:00:00

  • DNA methylome and transcriptome alterations and cancer prevention by curcumin in colitis-accelerated colon cancer in mice.

    abstract::Inflammation is highly associated with colon carcinogenesis. Epigenetic mechanisms could play an important role in the initiation and progression of colon cancer. Curcumin, a dietary phytochemical, shows promising effects in suppressing colitis-associated colon cancer in azoxymethane-dextran sulfate sodium (AOM-DSS) m...


    pub_type: 杂志文章


    authors: Guo Y,Wu R,Gaspar JM,Sargsyan D,Su ZY,Zhang C,Gao L,Cheng D,Li W,Wang C,Yin R,Fang M,Verzi MP,Hart RP,Kong AN

    更新日期:2018-05-03 00:00:00

  • Impact of multiple genetic polymorphisms on effects of a 4-week blueberry juice intervention on ex vivo induced lymphocytic DNA damage in human volunteers.

    abstract::Consumption of fruits and vegetables has been associated with a decrease in cancer incidence and cardiovascular disease, presumably caused by antioxidants. We designed a human intervention study to assess antioxidative and possible anti-genotoxic properties of fruit-borne antioxidants. We hypothesized that individuals...


    pub_type: 杂志文章,随机对照试验


    authors: Wilms LC,Boots AW,de Boer VC,Maas LM,Pachen DM,Gottschalk RW,Ketelslegers HB,Godschalk RW,Haenen GR,van Schooten FJ,Kleinjans JC

    更新日期:2007-08-01 00:00:00

  • Transgenic mice containing hepatitis B virus sequences are more susceptible to carcinogen-induced hepatocarcinogenesis.

    abstract::Transgenic mice containing one copy of hepatitis B virus (HBV) genome without the core gene and expressing hepatitis B surface antigen (HBsAg) were crossed with C3H/He mice. The F1 hybrids (approximately 50% HBV positive and approximately 50% HBV negative) were treated with a single dose of diethylnitrosamine (NDEA) o...


    pub_type: 杂志文章


    authors: Dragani TA,Manenti G,Farza H,Della Porta G,Tiollais P,Pourcel C

    更新日期:1990-06-01 00:00:00

  • The determination of urinary 3-methyladenine by immunoaffinity chromatography-monoclonal antibody-based ELISA: use in human biomonitoring studies.

    abstract::A mouse monoclonal antibody (Mab) was prepared which showed high specificity for a potential marker of exposure to methylating carcinogens such as 3-methyladenine (3-MeAde). In a low-temperature (4 degrees C) ELISA a linear calibration curve was obtained between 3 and 50 fmol/well. In combination with an immunoaffinit...


    pub_type: 杂志文章


    authors: Prevost V,Shuker DE,Bartsch H,Pastorelli R,Stillwell WG,Trudel LJ,Tannenbaum SR

    更新日期:1990-10-01 00:00:00

  • Mechanism of formation of the thioether conjugate of the bladder carcinogen 2-amino-4-(5-nitro-2-furyl)-thiazole (ANFT).

    abstract::The formation of thioether conjugates is an important pathway for inactivation of certain carcinogens. This study assessed the mechanism by which the bladder carcinogen 2-amino-4-(5-nitro-2-furyl)-thiazole (ANFT) forms a glutathione conjugate (ANFT-SG). Peroxidatic metabolism of ANFT, in the presence of glutathione, r...


    pub_type: 杂志文章


    authors: Lakshmi VM,Zenser TV,Sohani S,Davis BB

    更新日期:1992-11-01 00:00:00

  • Suppressive effects of nobiletin on hyperleptinemia and colitis-related colon carcinogenesis in male ICR mice.

    abstract::Adipocytokines are a group of adipocyte-secreted proteins that have significant effects on the metabolism of lipids and carbohydrates, as well as numerous other processes. A number of recent studies have indicated that some adipocytokines may significantly influence the proliferation of malignant cells in vitro, where...


    pub_type: 杂志文章


    authors: Miyamoto S,Yasui Y,Tanaka T,Ohigashi H,Murakami A

    更新日期:2008-05-01 00:00:00

  • The wavelength dependence of u.v.-induced pyrimidine dimer formation, cell killing and mutation induction in human diploid skin fibroblasts.

    abstract::In this study, we determined the wavelength dependence of u.v.-induced pyrimidine dimer formation, cell killing and mutation induction in human diploid skin fibroblasts. Pyrimidine dimers were quantified using the T4 endonuclease V assay, cell killing was measured as loss of colony forming ability and mutation inducti...


    pub_type: 杂志文章


    authors: Enninga IC,Groenendijk RT,Filon AR,van Zeeland AA,Simons JW

    更新日期:1986-11-01 00:00:00

  • Enhancement of the invasive ability of a transformed human bronchial epithelial cell line by 12-O-tetradecanoyl-phorbol-13-acetate and diacylglycerol.

    abstract::The effect of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) was studied using an immortalized human bronchial epithelial cell line, BEAS-2B, both in vivo and in vitro. The in vivo model consisted of tracheas reconstituted with an epithelium of BEAS-2B cells xenotransplanted into athymic nude mice. Int...


    pub_type: 杂志文章


    authors: Bonfil RD,Momiki S,Fridman R,Reich R,Reddel R,Harris CC,Klein-Szanto A

    更新日期:1989-12-01 00:00:00

  • Vinyl carbamate epoxide, a major strong electrophilic, mutagenic and carcinogenic metabolite of vinyl carbamate and ethyl carbamate (urethane).

    abstract::Vinyl carbamate epoxide (VCO) was found to possess strong electrophilic, mutagenic and carcinogenic activities. It reacted with water at 37 degrees C and pH 7.4 (phosphate buffer) to form glycolaldehyde and several related reducing compounds; none of these products were mutagenic for Salmonella typhimurium TA1535. Und...


    pub_type: 杂志文章


    authors: Park KK,Liem A,Stewart BC,Miller JA

    更新日期:1993-03-01 00:00:00

  • Lack of DNA damage or lipid peroxidation measured in vivo in the rat liver following treatment with peroxisomal proliferators.

    abstract::This study was undertaken to investigate the hypothesis linking peroxisome proliferation with the production of reactive oxygen species and subsequent DNA damage. Hepatic peroxisomal proliferation was induced in male Wistar-derived rats by the administration of clofibrate, methyl clofenapate, di(2-ethylhexyl)phthalate...


    pub_type: 杂志文章


    authors: Elliott BM,Elcombe CR

    更新日期:1987-09-01 00:00:00

  • Multistage chemical carcinogenesis protocols produce spindle cell carcinomas of the mouse skin.

    abstract::Spindle cell carcinomas were identified using polyacrylamide gel electrophoresis and immunoblotting of proteins extracted from paraffin-embedded tissue sections. Immunohistochemistry using rabbit monospecific antisera against the mouse 55 kd keratin polypeptide also identified these tumors. A group of 53 SENCAR mice i...


    pub_type: 杂志文章


    authors: Klein-Szanto AJ,Larcher F,Bonfil RD,Conti CJ

    更新日期:1989-11-01 00:00:00

  • Potent suppression of both spontaneous and carcinogen-induced colitis-associated colorectal cancer in mice by dietary celastrol supplementation.

    abstract::Celastrol is an anti-inflammatory natural triterpenoid, isolated from the herb Tripterygium wilfordii or thunder god vine. Here, we define mechanisms mediating anti-inflammatory activity of celastrol and demonstrate efficacy of a dietary celastrol supplement for chemoprevention of inflammation-driven carcinogenesis in...


    pub_type: 杂志文章


    authors: Barker EC,Kim BG,Yoon JH,Tochtrop GP,Letterio JJ,Choi SH

    更新日期:2018-01-12 00:00:00

  • Interaction of P53 Arg72Pro and MDM2 T309G polymorphisms and their associations with risk of gastric cardia cancer.

    abstract::The P53 tumor suppressor pathway plays an important role in cancer development. The auto-regulatory feedback mechanism of the P53 and MDM2 expression is critical in keeping proper tumor suppressor function of this pathway. This study examined the effect of P53 Arg72Pro variants on transactivation of polymorphic MDM2 p...


    pub_type: 杂志文章


    authors: Yang M,Guo Y,Zhang X,Miao X,Tan W,Sun T,Zhao D,Yu D,Liu J,Lin D

    更新日期:2007-09-01 00:00:00