Fibulin-5 is upregulated in decidualized human endometrial stromal cells and promotes primary human extravillous trophoblast outgrowth.


:Interactions between the highly invasive trophoblasts and the maternal uterine decidual extracellular matrix (ECM) are crucial in the determination of a successful pregnancy. Fibulin-5 (FBLN5) is a member of the fibulin family that alters cell adhesive and invasive properties and is expressed in human villous cytotrophoblasts. We aimed to determine the expression and immunolocalization of FBLN5 in human first trimester decidua and examine the effect of FBLN5 in trophoblast invasion in vitro using a first trimester placental villous outgrowth assay. We demonstrated that FBLN5 mRNA expression is upregulated in response to cAMP-mediated decidualization of primary human endometrial stromal cells, although FBLN5 itself does not enhance decidualization. We reported for the first time, FBLN5 protein production in first trimester decidual cells and also co-localization to HLAG-positive EVTs in first trimester decidua. Consequently, we investigated the effects of exogenous FBLN5 on placental villous outgrowth in vitro and demonstrated that FBLN5 promotes EVT migration/invasion. This is the first study to identify FBLN5 in decidualized human endometrial stromal cells, first trimester decidua and EVT and determine a functional role for FBLN5 in human EVTs, suggesting that decidual and or EVT-derived FBLN5 regulates EVT invasion and placentation in women.






Winship A,Cuman C,Rainczuk K,Dimitriadis E




Has Abstract


2015-12-01 00:00:00
















  • The Elsevier Trophoblast Research Award Lecture: Importance of metzincin proteases in trophoblast biology and placental development: a focus on ADAM12.

    abstract::Placental development is a highly regulated process requiring signals from both fetal and maternal uterine compartments. Within this complex system, trophoblasts, placental cells of epithelial lineage, form the maternal-fetal interface controlling nutrient, gas and waste exchange. The commitment of progenitor villous ...


    pub_type: 杂志文章,评审


    authors: Aghababaei M,Beristain AG

    更新日期:2015-04-01 00:00:00

  • Upregulation of VEGF by small activating RNA and its implications in preeclampsia.

    abstract:INTRODUCTION:Preeclampsia is a severe pregnancy complication mostly due to inadequate vascular dilation and remodeling of spiral arteries. VEGF, the major factor for angiogenesis, is necessary for modulating angiogenic processes in the placenta. Hence reduction of VEGF in gestational hypertension may also lead to hypop...


    pub_type: 杂志文章


    authors: Guo X,Feng L,Jia J,Chen R,Yu J

    更新日期:2016-10-01 00:00:00

  • Novel isolation strategy to deliver pure fetal-origin and maternal-origin mesenchymal stem cell (MSC) populations from human term placenta.

    abstract::The placenta is an abundant source of mesenchymal stem/stromal cells (MSC). Although presumed of translationally-advantageous fetal origin, the literature instead suggests a high incidence of either contaminating or pure maternal MSC. Despite definitional criteria that MSC are CD34-, increasing evidence suggests that ...


    pub_type: 杂志文章


    authors: Patel J,Shafiee A,Wang W,Fisk NM,Khosrotehrani K

    更新日期:2014-11-01 00:00:00

  • Cryopreservation of placental biopsies for mitochondrial respiratory analysis.

    abstract::Mitochondrial function is required to support energetically-demanding processes in the placenta. As such, a compromise in mitochondrial function could severely impact fetal growth and development. Respirometry is a highly useful method for studying mitochondrial function, but is not possible in freeze-thawed mitochond...


    pub_type: 杂志文章


    authors: Colleoni F,Morash AJ,Ashmore T,Monk M,Burton GJ,Murray AJ

    更新日期:2012-02-01 00:00:00

  • Leptin expression in the fetus and placenta during mouse pregnancy.

    abstract::During pregnancy, leptin concentrations in the maternal circulation are elevated in both humans and rodents but decrease to pre-pregnancy levels at birth, suggesting a role for leptin in the maintenance of pregnancy. Synthesis of leptin by the human placenta is established but whether the murine placenta synthesizes l...


    pub_type: 杂志文章


    authors: Malik NM,Carter ND,Wilson CA,Scaramuzzi RJ,Stock MJ,Murray JF

    更新日期:2005-01-01 00:00:00

  • Evidence for the functional activity of hypoxia-inducible transcription factors overexpressed in preeclamptic placentae.

    abstract::Placentas from women with preeclampsia overexpress the hypoxia-inducible transcription factor proteins, HIF-1alpha and -2alpha (Rajakumar 2001, Biol Reprod 64; p499-506 and p1019-1020). As a first step in evaluating whether HIF-alpha overexpressed in preeclamptic placentae is capable of transactivation, we tested its ...


    pub_type: 杂志文章


    authors: Rajakumar A,Brandon HM,Daftary A,Ness R,Conrad KP

    更新日期:2004-11-01 00:00:00

  • Region-specific changes in the mRNA and protein expression of LCPUFA biosynthesis enzymes and transporters in the placentae of women with preeclampsia.

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    pub_type: 杂志文章


    authors: Rani A,Chavan-Gautam P,Mehendale S,Wagh G,Mani NS,Joshi S

    更新日期:2020-06-01 00:00:00

  • Alphafetoprotein and alphafetoprotein receptor expression in the normal human placenta at term.

    abstract::Alphafetoprotein (AFP) is detectable in maternal serum from around six weeks of gestation and is synthesised by the yolk sac and the fetal liver. The role of the placenta in the transport and possible synthesis of AFP is uncertain. The aim of this study was to investigate placental expression of AFP and the AFP recept...


    pub_type: 杂志文章


    authors: Newby D,Dalgliesh G,Lyall F,Aitken DA

    更新日期:2005-02-01 00:00:00

  • Biological features of placental programming.

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    pub_type: 杂志文章,评审


    authors: Thornburg KL,Kolahi K,Pierce M,Valent A,Drake R,Louey S

    更新日期:2016-12-01 00:00:00

  • Specific infiltration pattern of FOXP3+ regulatory T cells in chronic histiocytic intervillositis of unknown etiology.

    abstract:INTRODUCTION:Chronic histiocytic intervillositis of unknown etiology (CIUE) is a rare placental lesion characterized by an intervillous mononuclear inflammatory infiltrate of maternal origin. Although the mechanism and origin of these lesions are currently not understood, they appear to be related to an immune conflict...


    pub_type: 杂志文章


    authors: Capuani C,Meggetto F,Duga I,Danjoux M,March M,Parant O,Brousset P,Aziza J

    更新日期:2013-02-01 00:00:00

  • Protective effect of hypotaurine against oxidative stress-induced cytotoxicity in rat placental trophoblasts.

    abstract:INTRODUCTION:Hypotaurine is a precursor of taurine and an antioxidant, and is concentrated in fetal plasma compared to maternal plasma. Hypotaurine is significantly decreased in fetal plasma of ezrin (Vil2) knock-out mice, and fetuses show intrauterine growth retardation. The aim of this study was to characterize the m...


    pub_type: 杂志文章


    authors: Nishimura T,Duereh M,Sugita Y,Yoshida Y,Higuchi K,Tomi M,Nakashima E

    更新日期:2015-06-01 00:00:00

  • Nitric oxide (NO) reversed TNF-α inhibition of trophoblast interaction with endothelial cellular networks.

    abstract:INTRODUCTION:The interaction between trophoblast cells and maternal uterine endothelium is important for placental vascular modelling. Nitric oxide (NO) is a potent vasorelaxant that regulates systemic blood pressure and is reduced in preeclampsia. NO may affect placental cell interaction. OBJECTIVES:This study was to...


    pub_type: 杂志文章


    authors: Xu B,Charlton F,Makris A,Hennessy A

    更新日期:2014-06-01 00:00:00

  • The human placenta is encircled by a ring of smooth muscle cells.

    abstract::The marginal zone of the human term placenta was studied by transmission electron microscopy and immunohistochemistry using antibodies against cytoskeletal filaments, extracellular matrix molecules and endothelial markers. The marginal sinus of the intervillous space is separated from the chorionic and basal plates by...


    pub_type: 杂志文章


    authors: Nanaev AK,Kosanke G,Kemp B,Frank HG,Huppertz B,Kaufmann P

    更新日期:2000-01-01 00:00:00

  • Increased matrix metalloproteinases 2 and 9 in placenta of diabetic rats at midgestation.

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    pub_type: 杂志文章


    authors: Pustovrh MC,Jawerbaum A,Capobianco E,White V,López-Costa JJ,González E

    更新日期:2005-04-01 00:00:00

  • Protection of murine gestational tissues from picornavirus infection in the preimplantation period.

    abstract::Mice were inoculated with Theiler's murine encephalomyelitis virus (TMEV) on gestational days 1-3 (pre-implantation) or days 4-5 (peri- or post-implantation) or with control cell lysate (days 1-5). Dams were subsequently sacrificed between days 11-14 of gestation, and placentae and fetuses were harvested. Few placenta...


    pub_type: 杂志文章


    authors: Abzug MJ,Tyson RW

    更新日期:2000-05-01 00:00:00

  • Fibrinous vasculosis in the human placenta.

    abstract::Approximately 6 per cent of placentae of babies admitted to a special care paediatric unit show evidence of peripheral villous stem branch oedema. In more than half of these cases there is evidence of fibrinous vasculosis (FV) in truncal veins. The affected placentae are generally thick and of small diameter, often of...


    pub_type: 杂志文章


    authors: Scott JM

    更新日期:1983-01-01 00:00:00

  • The function of miR-519d in cell migration, invasion, and proliferation suggests a role in early placentation.

    abstract::The processes of proliferation, migration, and invasion of extravillous trophoblasts are critical for placental implantation and early development, and directly influence pregnancy outcome. Dysregulation of these processes has been associated with placental dysfunction, implicated in clinical conditions such as preecl...


    pub_type: 杂志文章,评审


    authors: Xie L,Sadovsky Y

    更新日期:2016-12-01 00:00:00

  • In vitro fertilization is associated with the onset and progression of preeclampsia.

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    pub_type: 杂志文章


    authors: Gui J,Ling Z,Hou X,Fan Y,Xie K,Shen R

    更新日期:2020-01-01 00:00:00

  • A function of the allantoic sac in the metabolism of uteroferrin and maternal iron by the fetal pig.

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    pub_type: 杂志文章


    authors: Buhi WC,Ducsay CA,Bartol FF,Bazer FW,Roberts RM

    更新日期:1983-01-01 00:00:00

  • Alpha-fetoprotein gene expression in early and full-term human trophoblast.

    abstract::Alpha-fetoprotein (AFP) is a major serum glycoprotein synthesized during fetal life mainly by the yolk sac and the fetal liver. At term, it reaches high concentrations in the maternal intervillous blood, which is in direct contact with the placental trophoblastic microvillous membrane, and this suggests the placental ...


    pub_type: 杂志文章


    authors: Lafuste P,Robert B,Mondon F,Danan JL,Rossi B,Duc-Goiran P,Mignot TM,Nunez EA,Benassayag C,Ferré F

    更新日期:2002-09-01 00:00:00

  • Aberrations of early trophoblast differentiation predispose to pregnancy failure: lessons from the anti-phospholipid syndrome.

    abstract:OBJECTIVES:The epithelium of the human placenta comprises an inner cytotrophoblast (CT) which proliferates and fuses with the outer differentiated syncytiotrophoblast (ST). Turnover has been studied focussing on second and third trimester placentas but with a paucity of data describing the normal first trimester tropho...


    pub_type: 杂志文章


    authors: Bose P,Kadyrov M,Goldin R,Hahn S,Backos M,Regan L,Huppertz B

    更新日期:2006-08-01 00:00:00

  • The Role of E-cadherin in the Motility and Invasion of Implantation Site Intermediate Trophoblast.

    abstract::During early pregnancy, intermediate (extravillous) trophoblast infiltrates the basal plate and invades the spiral arteries, a physiological process required to establish the maternal-fetal circulation. Immunostaining studies have shown that differentiation of trophoblast into this invasive subpopulation is associated...


    pub_type: 杂志文章


    authors: Shih IeM,Hsu MY,Oldt RJ 3rd,Herlyn M,Gearhart JD,Kurman RJ

    更新日期:2002-11-01 00:00:00

  • Monoclonal antibody defining a human syncytiotrophoblastic polypeptide immunologically related to mammalian retrovirus structural protein p30.

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    pub_type: 杂志文章


    authors: Wahlström T,Nieminen P,Närvänen A,Suni J,Lehtovirta P,Saksela E,Vaheri A

    更新日期:1984-11-01 00:00:00

  • Klf14 is an imprinted transcription factor that regulates placental growth.

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    pub_type: 杂志文章


    authors: Koppes E,Shaffer B,Sadovsky E,Himes K,Barak Y,Sadovsky Y,Chaillet JR

    更新日期:2019-12-01 00:00:00

  • Apoptotic activity in villous trophoblast cells during B19 infection correlates with clinical outcome: assessment by the caspase-related M30 Cytodeath antibody.

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    pub_type: 杂志文章


    authors: Jordan JA,Butchko AR

    更新日期:2002-08-01 00:00:00

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    pub_type: 杂志文章


    authors: Liu H,Kang M,Wang J,Blenkiron C,Lee A,Wise M,Chamley L,Chen Q

    更新日期:2018-12-01 00:00:00

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    pub_type: 杂志文章


    authors: Kwan M,Hazan A,Zhang J,Jones RL,Harris LK,Whittle W,Keating S,Dunk CE,Lye SJ

    更新日期:2014-12-01 00:00:00

  • Placental localization of 15-hydroxy-prostaglandin dehydrogenase in early and term human pregnancy.

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    pub_type: 杂志文章


    authors: Erwich JJ,Keirse MJ

    更新日期:1992-05-01 00:00:00

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    abstract::Effects of placental growth factor (PGF), an angiokine product of fetal trophoblasts and maternal decidual cells, on early decidual angiogenesis are undefined. We used whole-mount immunofluorescence analyses to compare uterus and gestation day 4.5-9.5 mouse implantation sites that differed genetically in fetal or mate...


    pub_type: 杂志文章


    authors: Rätsep MT,Carmeliet P,Adams MA,Croy BA

    更新日期:2014-09-01 00:00:00

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    pub_type: 杂志文章


    authors: Lefebvre S,Moreau P,Dausset J,Carosella ED,Paul P

    更新日期:1999-05-01 00:00:00