Characterization of DNA Methylation in Circulating Tumor Cells.


:Epigenetics contributes to molecular mechanisms leading to tumor cell transformation and systemic progression of cancer. However, the dynamics of epigenetic remodeling during metastasis remains unexplored. In this context, circulating tumor cells (CTCs) might enable a direct insight into epigenetic mechanisms relevant for metastasis by providing direct access to systemic cancer. CTCs can be used as prognostic markers in cancer patients and are regarded as potential metastatic precursor cells. However, despite substantial technical progress, the detection and molecular characterization of CTCs remain challenging, in particular the analysis of DNA methylation. As recent studies have started to address the epigenetic state of CTCs, we discuss here the potential of such investigations to elucidate mechanisms of metastasis and to develop tumor biomarkers.


Genes (Basel)




Pixberg CF,Schulz WA,Stoecklein NH,Neves RP




Has Abstract


2015-10-21 00:00:00














  • Allelic imbalances in radiation-associated acute myeloid leukemia.

    abstract::Acute myeloid leukemia (AML) can develop as a secondary malignancy following radiotherapy, but also following low-dose environmental or occupational radiation exposure. Therapy-related AML frequently carries deletions of chromosome 5q and/or 7, but for low-dose exposure associated AML this has not been described. For ...


    pub_type: 杂志文章


    authors: Klymenko SV,Smida J,Atkinson MJ,Bebeshko VG,Nathrath M,Rosemann M

    更新日期:2011-05-31 00:00:00

  • Impact, Characterization, and Rescue of Pre-mRNA Splicing Mutations in Lysosomal Storage Disorders.

    abstract::Lysosomal storage disorders (LSDs) represent a group of more than 50 severe metabolic diseases caused by the deficiency of specific lysosomal hydrolases, activators, carriers, or lysosomal integral membrane proteins, leading to the abnormal accumulation of substrates within the lysosomes. Numerous mutations have been ...


    pub_type: 杂志文章,评审


    authors: Dardis A,Buratti E

    更新日期:2018-02-06 00:00:00

  • Unravelling the Roles of Susceptibility Loci for Autoimmune Diseases in the Post-GWAS Era.

    abstract::Although genome-wide association studies (GWAS) have identified several hundred loci associated with autoimmune diseases, their mechanistic insights are still poorly understood. The human genome is more complex than single nucleotide polymorphisms (SNPs) that are interrogated by GWAS arrays. Apart from SNPs, it also c...


    pub_type: 杂志文章,评审


    authors: Ye J,Gillespie KM,Rodriguez S

    更新日期:2018-07-27 00:00:00

  • Off Earth Identification of Bacterial Populations Using 16S rDNA Nanopore Sequencing.

    abstract::The MinION sequencer has made in situ sequencing feasible in remote locations. Following our initial demonstration of its high performance off planet with Earth-prepared samples, we developed and tested an end-to-end, sample-to-sequencer process that could be conducted entirely aboard the International Space Station (...


    pub_type: 杂志文章


    authors: Burton AS,Stahl SE,John KK,Jain M,Juul S,Turner DJ,Harrington ED,Stoddart D,Paten B,Akeson M,Castro-Wallace SL

    更新日期:2020-01-09 00:00:00

  • Study of mcr-1 Gene-Mediated Colistin Resistance in Enterobacteriaceae Isolated from Humans and Animals in Different Countries.

    abstract::In this study, we aim to characterize the genetic environment of the plasmid-mediated colistin resistance gene mcr-1 in 25 Escherichia coli and seven Klebsiella pneumoniae strains from different countries and continents. Multilocus sequence typing, conjugation experiments, plasmid typing, and the presence and location...


    pub_type: 杂志文章


    authors: Hadjadj L,Riziki T,Zhu Y,Li J,Diene SM,Rolain JM

    更新日期:2017-12-19 00:00:00

  • PTEN Alterations and Their Role in Cancer Management: Are We Making Headway on Precision Medicine?

    abstract::Alterations in the tumor suppressor phosphatase and tensin homolog (PTEN) occur in a substantial proportion of solid tumors. These events drive tumorigenesis and tumor progression. Given its central role as a downregulator of the phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway, PTEN i...


    pub_type: 杂志文章,评审


    authors: Fusco N,Sajjadi E,Venetis K,Gaudioso G,Lopez G,Corti C,Rocco EG,Criscitiello C,Malapelle U,Invernizzi M

    更新日期:2020-06-28 00:00:00

  • The role of bromodomain proteins in regulating gene expression.

    abstract::Histone modifications are important in regulating gene expression in eukaryotes. Of the numerous histone modifications which have been identified, acetylation is one of the best characterised and is generally associated with active genes. Histone acetylation can directly affect chromatin structure by neutralising char...


    pub_type: 杂志文章


    authors: Josling GA,Selvarajah SA,Petter M,Duffy MF

    更新日期:2012-05-29 00:00:00

  • miR-101b Regulates Lipid Deposition and Metabolism of Primary Hepatocytes in Teleost Yellow Catfish Pelteobagrus fulvidraco.

    abstract::Excessive fat deposition in the hepatocytes, associated with excess dietary fat intake, was related to the occurrence of fatty livers in fish. miR-101b plays the important roles in controlling lipid metabolism, but the underlying mechanism at the post-transcriptional level remains unclear. The purpose of this study is...


    pub_type: 杂志文章


    authors: Chen GH,Zhao T,Wei XL,Zhang DG,Zhuo MQ,Luo Z

    更新日期:2020-07-29 00:00:00

  • Genome Sequencing and Assembly by Long Reads in Plants.

    abstract::Plant genomes generated by Sanger and Next Generation Sequencing (NGS) have provided insight into species diversity and evolution. However, Sanger sequencing is limited in its applications due to high cost, labor intensity, and low throughput, while NGS reads are too short to resolve abundant repeats and polyploidy, l...


    pub_type: 杂志文章,评审


    authors: Li C,Lin F,An D,Wang W,Huang R

    更新日期:2017-12-28 00:00:00

  • Regulation of the X Chromosome in the Germline and Soma of Drosophila melanogaster Males.

    abstract::During the evolution of heteromorphic sex chromosomes, the sex-specific Y chromosome degenerates, while the X chromosome evolves new mechanisms of regulation. Using bioinformatic and experimental approaches, we investigate the expression of the X chromosome in Drosophila melanogaster. We observe nearly complete X chro...


    pub_type: 杂志文章


    authors: Argyridou E,Parsch J

    更新日期:2018-05-04 00:00:00

  • Characterization of Copy-Number Variations and Possible Candidate Genes in Recurrent Pregnancy Losses.

    abstract::It is well established that embryonic chromosomal abnormalities (both in the number of chromosomes and the structure) account for 50% of early pregnancy losses. However, little is known regarding the potential differences in the incidence and distribution of chromosomal abnormalities between patients with sporadic abo...


    pub_type: 杂志文章


    authors: Sheng YR,Hou SY,Hu WT,Wei CY,Liu YK,Liu YY,Jiang L,Xiang JJ,Sun XX,Lei CX,Wang HL,Zhu XY

    更新日期:2021-01-22 00:00:00

  • Nucleolar Expression and Chromosomal Associations in Robertsonian Spermatocytes of Mus musculus domesticus.

    abstract::We studied and compared the nucleolar expression or nucleoli from specific bivalents in spermatocytes of the standard Mus musculus domesticus 2n=40, of Robertsonian (Rb) homozygotes 2n = 24 and heterozygotes 2n = 32. We analyzed 200 nuclear microspreads of each specific nucleolar chromosome and spermatocyte karyotype,...


    pub_type: 杂志文章


    authors: López-Moncada F,Tapia D,Zuñiga N,Ayarza E,López-Fenner J,Redi CA,Berríos S

    更新日期:2019-02-06 00:00:00

  • Comparison of Illumina versus Nanopore 16S rRNA Gene Sequencing of the Human Nasal Microbiota.

    abstract::Illumina and nanopore sequencing technologies are powerful tools that can be used to determine the bacterial composition of complex microbial communities. In this study, we compared nasal microbiota results at genus level using both Illumina and nanopore 16S rRNA gene sequencing. We also monitored the progression of n...


    pub_type: 杂志文章


    authors: Heikema AP,Horst-Kreft D,Boers SA,Jansen R,Hiltemann SD,de Koning W,Kraaij R,de Ridder MAJ,van Houten CB,Bont LJ,Stubbs AP,Hays JP

    更新日期:2020-09-21 00:00:00

  • Biodegradation of Tetralin: Genomics, Gene Function and Regulation.

    abstract::Tetralin (1,2,3,4-tetrahydonaphthalene) is a recalcitrant compound that consists of an aromatic and an alicyclic ring. It is found in crude oils, produced industrially from naphthalene or anthracene, and widely used as an organic solvent. Its toxicity is due to the alteration of biological membranes by its hydrophobic...


    pub_type: 杂志文章,评审


    authors: Floriano B,Santero E,Reyes-Ramírez F

    更新日期:2019-05-06 00:00:00

  • Novel Variant in PLAG1 in a Familial Case with Silver-Russell Syndrome Suspicion.

    abstract::Silver-Russell syndrome (SRS) is a rare growth-related genetic disorder that is mainly associated with prenatal and postnatal growth retardation. Molecular causes are not clear in all cases, the most common ones being loss of methylation on chromosome 11p15 (≈50%) and maternal uniparental disomy for chromosome 7 (upd(...




    authors: Vado Y,Pereda A,Llano-Rivas I,Gorria-Redondo N,Díez I,Perez de Nanclares G

    更新日期:2020-12-05 00:00:00

  • DNA Barcoding Reveals High Levels of Divergence among Mitochondrial Lineages of Brycon (Characiformes, Bryconidae).

    abstract::Brycon is an important group of Neotropical fish and the principal genus of the family Bryconidae, with 44 valid species that are found in some Central American rivers and practically all the major hydrographic basins of South America. These fish are medium to large in size, migratory, omnivorous, important seed dispe...


    pub_type: 杂志文章


    authors: Arruda PSS,Ferreira DC,Oliveira C,Venere PC

    更新日期:2019-08-23 00:00:00

  • Two New Cases of Hypertrophic Cardiomyopathy and Skeletal Muscle Features Associated with ALPK3 Homozygous and Compound Heterozygous Variants.

    abstract::Hypertrophic cardiomyopathy associated with damaging variants in the ALPK3 gene is a fairly recent discovery, and only a small number of patients have been described thus far. Here we present two additional patients with hypertrophic cardiomyopathy caused by biallelic variants in ALPK3. Genetic investigation was perfo...


    pub_type: 杂志文章


    authors: Jorholt J,Formicheva Y,Vershinina T,Kiselev A,Muravyev A,Demchenko E,Fedotov P,Zlotina A,Rygkov A,Vasichkina E,Sejersen T,Kostareva A

    更新日期:2020-10-15 00:00:00

  • Age-Related Epigenetic Derangement upon Reprogramming and Differentiation of Cells from the Elderly.

    abstract::Aging is a complex multi-layered phenomenon. The study of aging in humans is based on the use of biological material from hard-to-gather tissues and highly specific cohorts. The introduction of cell reprogramming techniques posed promising features for medical practice and basic research. Recently, a growing number of...


    pub_type: 杂志文章,评审


    authors: Ravaioli F,Bacalini MG,Franceschi C,Garagnani P

    更新日期:2018-01-16 00:00:00

  • Two Separate Cases: Complex Chromosomal Abnormality Involving Three Chromosomes and Small Supernumerary Marker Chromosome in Patients with Impaired Reproductive Function.

    abstract::For medical genetic counseling, estimating the chance of a child being born with chromosome abnormality is crucially important. Cytogenetic diagnostics of parents with a balanced karyotype are a special case. Such chromosome rearrangements cannot be detected with comprehensive chromosome screening. In the current pape...


    pub_type: 杂志文章


    authors: Karamysheva TV,Gayner TA,Muzyka VV,Orishchenko KE,Rubtsov NB

    更新日期:2020-12-17 00:00:00

  • Network-Based Approaches to Explore Complex Biological Systems towards Network Medicine.

    abstract::Network medicine relies on different types of networks: from the molecular level of protein⁻protein interactions to gene regulatory network and correlation studies of gene expression. Among network approaches based on the analysis of the topological properties of protein⁻protein interaction (PPI) networks, we discuss ...


    pub_type: 杂志文章,评审


    authors: Fiscon G,Conte F,Farina L,Paci P

    更新日期:2018-08-31 00:00:00

  • Coping with Reactive Oxygen Species to Ensure Genome Stability in Escherichia coli.

    abstract::The facultative aerobic bacterium Escherichia coli adjusts its cell cycle to environmental conditions. Because of its lifestyle, the bacterium has to balance the use of oxygen with the potential lethal effects of its poisonous derivatives. Oxidative damages perpetrated by molecules such as hydrogen peroxide and supero...


    pub_type: 杂志文章,评审


    authors: Mendoza-Chamizo B,Løbner-Olesen A,Charbon G

    更新日期:2018-11-21 00:00:00

  • Testosterone Degradative Pathway of Novosphingobium tardaugens.

    abstract::In this work, we have shown that Novosphingobium tardaugens NBRC 16725 (strain ARI-1), a bacterial strain that was isolated due to its capacity to mineralize the estrogenic endocrine compound 17β-estradiol, is also able to mineralize testosterone, the androgenic endocrine compound. Using in silico analysis, we predict...


    pub_type: 杂志文章


    authors: Ibero J,Galán B,Díaz E,García JL

    更新日期:2019-10-31 00:00:00

  • The Genome of the North American Brown Bear or Grizzly: Ursus arctos ssp. horribilis.

    abstract::The grizzly bear (Ursus arctos ssp. horribilis) represents the largest population of brown bears in North America. Its genome was sequenced using a microfluidic partitioning library construction technique, and these data were supplemented with sequencing from a nanopore-based long read platform. The final assembly was...


    pub_type: 杂志文章


    authors: Taylor GA,Kirk H,Coombe L,Jackman SD,Chu J,Tse K,Cheng D,Chuah E,Pandoh P,Carlsen R,Zhao Y,Mungall AJ,Moore R,Birol I,Franke M,Marra MA,Dutton C,Jones SJM

    更新日期:2018-11-30 00:00:00

  • Circulating miR-206 as a Biomarker for Patients Affected by Severe Limb Girdle Muscle Dystrophies.

    abstract::Limb-girdle muscular dystrophies (LGMD) are clinically and genetically heterogeneous conditions, presenting with a wide clinical spectrum, leading to progressive proximal weakness caused by loss of muscle fibers. MiR-206 is a member of myomiRNAs, a group of miRNAs with important function in skeletal muscle. Our aim is...


    pub_type: 杂志文章


    authors: Pegoraro V,Angelini C

    更新日期:2021-01-12 00:00:00

  • Multigene Expression Assay for Assessment of the Immune Status of Atlantic Salmon.

    abstract::We report the development of a multigene gene expression assay on the BioMark HD platform for the evaluation of immune competence (ImCom) in farmed Atlantic salmon. The first version of the assay included 92 genes selected on the basis of transcriptome analyses in 54 trials that challenged the immune system; annotatio...


    pub_type: 杂志文章


    authors: Krasnov A,Afanasyev S,Nylund S,Rebl A

    更新日期:2020-10-22 00:00:00

  • Golgi Reassembly and Stacking Protein (GRASP) Participates in Vesicle-Mediated RNA Export in Cryptococcus Neoformans.

    abstract::Golgi reassembly and stacking protein (GRASP) is required for polysaccharide secretion and virulence in Cryptococcus neoformans. In fungal species, extracellular vesicles (EVs) participate in the export of polysaccharides, proteins and RNA. In the present work, we investigated if EV-mediated RNA export is functionally...


    pub_type: 杂志文章


    authors: Peres da Silva R,Martins ST,Rizzo J,Dos Reis FCG,Joffe LS,Vainstein M,Kmetzsch L,Oliveira DL,Puccia R,Goldenberg S,Rodrigues ML,Alves LR

    更新日期:2018-08-08 00:00:00

  • Molecular Strategies for RPGR Gene Therapy.

    abstract::Mutations affecting the Retinitis Pigmentosa GTPase Regulator (RPGR) gene are the commonest cause of X-linked and recessive retinitis pigmentosa (RP), accounting for 10%-20% of all cases of RP. The phenotype is one of the most severe amongst all causes of RP, characteristic for its early onset and rapid progression to...


    pub_type: 杂志文章,评审


    authors: Cehajic Kapetanovic J,McClements ME,Martinez-Fernandez de la Camara C,MacLaren RE

    更新日期:2019-09-04 00:00:00

  • Expanding the Clinical and Mutational Spectrum of Recessive AEBP1-Related Classical-Like Ehlers-Danlos Syndrome.

    abstract::Ehlers-Danlos syndrome (EDS) comprises clinically heterogeneous connective tissue disorders with diverse molecular etiologies. The 2017 International Classification for EDS recognized 13 distinct subtypes caused by pathogenic variants in 19 genes mainly encoding fibrillar collagens and collagen-modifying or processing...


    pub_type: 杂志文章


    authors: Ritelli M,Cinquina V,Venturini M,Pezzaioli L,Formenti AM,Chiarelli N,Colombi M

    更新日期:2019-02-12 00:00:00

  • Gene Expression Profile in Similar Tissues Using Transcriptome Sequencing Data of Whole-Body Horse Skeletal Muscle.

    abstract::Horses have been studied for exercise function rather than food production, unlike most livestock. Therefore, the role and characteristics of tissue landscapes are critically understudied, except for certain muscles used in exercise-related studies. In the present study, we compared RNA-Seq data from 18 Jeju horse ske...


    pub_type: 杂志文章


    authors: Lee HY,Kim JY,Kim KH,Jeong S,Cho Y,Kim N

    更新日期:2020-11-17 00:00:00

  • A Pan-Genome Guided Metabolic Network Reconstruction of Five Propionibacterium Species Reveals Extensive Metabolic Diversity.

    abstract::Propionibacteria have been studied extensively since the early 1930s due to their relevance to industry and importance as human pathogens. Still, their unique metabolism is far from fully understood. This is partly due to their signature high GC content, which has previously hampered the acquisition of quality sequenc...


    pub_type: 杂志文章


    authors: McCubbin T,Gonzalez-Garcia RA,Palfreyman RW,Stowers C,Nielsen LK,Marcellin E

    更新日期:2020-09-23 00:00:00