Absence of Radial Spokes in Mouse Node Cilia Is Required for Rotational Movement but Confers Ultrastructural Instability as a Trade-Off.

Abstract:

:Determination of left-right asymmetry in mouse embryos is established by a leftward fluid flow that is generated by clockwise rotation of node cilia. How node cilia achieve stable unidirectional rotation has remained unknown, however. Here we show that brief exposure to the microtubule-stabilizing drug paclitaxel (Taxol) induces randomly directed rotation and changes the ultrastructure of node cilia. In vivo observations and a computer simulation revealed that a regular 9+0 arrangement of doublet microtubules is essential for stable unidirectional rotation of node cilia. The 9+2 motile cilia of the airway, which manifest planar beating, are resistant to Taxol treatment. However, the airway cilia of mice lacking the radial spoke head protein Rsph4a undergo rotational movement instead of planar beating, are prone to microtubule rearrangement, and are sensitive to Taxol. Our results suggest that the absence of radial spokes allows node cilia to rotate unidirectionally but, as a trade-off, renders them ultrastructurally fragile.

journal_name

Dev Cell

journal_title

Developmental cell

authors

Shinohara K,Chen D,Nishida T,Misaki K,Yonemura S,Hamada H

doi

10.1016/j.devcel.2015.10.001

subject

Has Abstract

pub_date

2015-10-26 00:00:00

pages

236-46

issue

2

eissn

1534-5807

issn

1878-1551

pii

S1534-5807(15)00628-0

journal_volume

35

pub_type

杂志文章
  • Muscle building; mechanisms of myotube guidance and attachment site selection.

    abstract::The complex muscle patterns of higher organisms arise as migrating myoblasts are guided toward and connect with specific attachment sites. We review here the current understanding of myotube migration, focusing on its dynamic nature and the few molecular cues that have been identified to date. Much of this knowledge c...

    journal_title:Developmental cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.devcel.2004.06.010

    authors: Schnorrer F,Dickson BJ

    更新日期:2004-07-01 00:00:00

  • Neonatal β cell development in mice and humans is regulated by calcineurin/NFAT.

    abstract::Little is known about the mechanisms governing neonatal growth and maturation of organs. Here we demonstrate that calcineurin/Nuclear Factor of Activated T cells (Cn/NFAT) signaling regulates neonatal pancreatic development in mouse and human islets. Inactivation of calcineurin b1 (Cnb1) in mouse islets impaired dense...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2012.05.014

    authors: Goodyer WR,Gu X,Liu Y,Bottino R,Crabtree GR,Kim SK

    更新日期:2012-07-17 00:00:00

  • The role of GDNF/Ret signaling in ureteric bud cell fate and branching morphogenesis.

    abstract::While GDNF signaling through the Ret receptor is critical for kidney development, its specific role in branching morphogenesis of the epithelial ureteric bud (UB) is unclear. Ret expression defines a population of UB "tip cells" distinct from cells of the tubular "trunks," but how these cells contribute to UB growth i...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2004.11.008

    authors: Shakya R,Watanabe T,Costantini F

    更新日期:2005-01-01 00:00:00

  • The PAR-6 polarity protein regulates dendritic spine morphogenesis through p190 RhoGAP and the Rho GTPase.

    abstract::The majority of excitatory synaptic transmission in the brain occurs at dendritic spines, which are actin-rich protrusions on the dendrites. The asymmetric nature of these structures suggests that proteins regulating cell polarity might be involved in their formation. Indeed, the polarity protein PAR-3 is required for...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2007.11.020

    authors: Zhang H,Macara IG

    更新日期:2008-02-01 00:00:00

  • Less Is More: Ditching Mitochondria Saves Hypoxic Cartilage.

    abstract::In this issue of Developmental Cell, Yao et al. (2019) disrupt mitochondrial respiration in limb skeletal progenitors by conditionally deleting Tfam in the mouse embryo. They discover that not only is mitochondrial respiration dispensable for embryonic cartilage development, but also its elimination obviates the need ...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2019.05.030

    authors: Long F

    更新日期:2019-06-03 00:00:00

  • The undiscovered country: chromosome territories and the organization of transcription.

    abstract::The interchromosome domain (ICD) model proposes that genes are selectively positioned at the surfaces of chromosome territories to facilitate their regulation. A paper in the May 13 issue of the Journal of Cell Biology provides evidence that supports a reinterpretation of this model. ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/s1534-5807(02)00196-x

    authors: Kosak ST,Groudine M

    更新日期:2002-06-01 00:00:00

  • A Mad that wears two hats: Mad1's control of nuclear trafficking.

    abstract::In a recent issue of Molecular Cell, Cairo et al. (2013) report that the spindle checkpoint protein Mad1 shuttles between unattached kinetochores and nuclear pores. Mobile Mad1 inhibits the import receptor Kap121p, thus indirectly changing spindle dynamics. This adds nuclear transport control to the mitotic roles of M...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2013.01.003

    authors: Dasso M

    更新日期:2013-01-28 00:00:00

  • Organizing Complex Tissue Architecture by Pushing and Pulling Cell Contacts.

    abstract::Changes to cell shape and interaction drive animal tissue morphogenesis. Reporting now in Developmental Cell, Del Signore et al. (2018) describe active lengthening of cell-cell contacts by Arp2/3-based actin networks. Through cycles of contact lengthening and shortening, the developing Drosophila eye passes through va...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2018.02.003

    authors: Zhang Y,Harris TJC

    更新日期:2018-02-26 00:00:00

  • The mammalian passenger protein TD-60 is an RCC1 family member with an essential role in prometaphase to metaphase progression.

    abstract::Passenger proteins migrate from inner centromeres to the spindle midzone during late mitosis, and those described to date are essential both for proper chromosome segregation and for completion of cell cleavage. We have purified and cloned the human passenger protein TD-60, and we here report that it is a member of th...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/s1534-5807(03)00205-3

    authors: Mollinari C,Reynaud C,Martineau-Thuillier S,Monier S,Kieffer S,Garin J,Andreassen PR,Boulet A,Goud B,Kleman JP,Margolis RL

    更新日期:2003-08-01 00:00:00

  • Peroxiredoxin stabilization of DE-cadherin promotes primordial germ cell adhesion.

    abstract::Regulated adhesion between cells and their environment is critical for normal cell migration. We have identified mutations in a gene encoding the Drosophila hydrogen peroxide (H₂O₂)-degrading enzyme Jafrac1, which lead to germ cell adhesion defects. During gastrulation, primordial germ cells (PGCs) associate tightly w...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2010.12.007

    authors: DeGennaro M,Hurd TR,Siekhaus DE,Biteau B,Jasper H,Lehmann R

    更新日期:2011-02-15 00:00:00

  • Cell autonomy of HIF effects in Drosophila: tracheal cells sense hypoxia and induce terminal branch sprouting.

    abstract::Drosophila tracheal terminal branches are plastic and have the capacity to sprout out projections toward oxygen-starved areas, in a process analogous to mammalian angiogenesis. This response involves the upregulation of FGF/Branchless in hypoxic tissues, which binds its receptor Breathless on tracheal cells. Here, we ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2008.01.020

    authors: Centanin L,Dekanty A,Romero N,Irisarri M,Gorr TA,Wappner P

    更新日期:2008-04-01 00:00:00

  • ER Stress Leaves an Inflammatory TRAIL.

    abstract::Cell stress often triggers inflammation as a protective response to tissue damage or infection. In this issue of Developmental Cell, Sullivan et al. (2020) reveal an unexpected role for TRAIL receptors in ER-stress-induced inflammation. ...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2020.03.004

    authors: Roca Portoles A,Tait SWG

    更新日期:2020-03-23 00:00:00

  • SAG/RBX2/ROC2 E3 ubiquitin ligase is essential for vascular and neural development by targeting NF1 for degradation.

    abstract::SAG/RBX2/ROC2 protein is an essential RING component of SCF E3 ubiquitin ligase. The role of SAG during embryogenesis remains unknown. We report a critical role for SAG in controlling vascular and neural development by modulating RAS activity via promoting degradation of neurofibromatosis type 1 (NF1). Mice mutant for...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2011.09.014

    authors: Tan M,Zhao Y,Kim SJ,Liu M,Jia L,Saunders TL,Zhu Y,Sun Y

    更新日期:2011-12-13 00:00:00

  • Hox and Pbx factors control retinoic acid synthesis during hindbrain segmentation.

    abstract::In vertebrate embryos, retinoic acid (RA) synthesized in the mesoderm by Raldh2 emanates to the hindbrain neuroepithelium, where it induces anteroposterior (AP)-restricted Hox expression patterns and rhombomere segmentation. However, how appropriate spatiotemporal RA activity is generated in the hindbrain is poorly un...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2011.03.011

    authors: Vitobello A,Ferretti E,Lampe X,Vilain N,Ducret S,Ori M,Spetz JF,Selleri L,Rijli FM

    更新日期:2011-04-19 00:00:00

  • RA Gets Out of the Way to Allow High-Acuity Vision.

    abstract::Specialized areas in the vertebrate retina are critical for high-acuity vision, yet the molecular mechanisms driving the development of high-acuity areas (HAAs) remain largely unknown. In Developmental Cell, da Silva and Cepko (2017) show that restricted degradation of retinoic acid and elevated FGF8 signaling give ri...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2017.06.015

    authors: Tufford A,Cayouette M

    更新日期:2017-07-10 00:00:00

  • MT1-MMP-dependent control of skeletal stem cell commitment via a β1-integrin/YAP/TAZ signaling axis.

    abstract::In vitro, topographical and biophysical cues arising from the extracellular matrix (ECM) direct skeletal stem cell (SSC) commitment and differentiation. However, the mechanisms by which the SSC-ECM interface is regulated and the outcome of such interactions on stem cell fate in vivo remain unknown. Here we demonstrate...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2013.04.011

    authors: Tang Y,Rowe RG,Botvinick EL,Kurup A,Putnam AJ,Seiki M,Weaver VM,Keller ET,Goldstein S,Dai J,Begun D,Saunders T,Weiss SJ

    更新日期:2013-05-28 00:00:00

  • Coping with Loss: Cell Adaptation to Cytoskeleton Disruption.

    abstract::Unravelling the role of cytoskeleton regulators may be complicated by adaptations to experimental manipulations. In this issue of Developmental Cell, Cerikan et al. (2016) reveal how acute effects of DOCK6 RhoGEF depletion on RAC1 and CDC42 activation are reversed over time by compensatory mechanisms that re-establish...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2016.09.020

    authors: McGarry DJ,Olson MF

    更新日期:2016-10-10 00:00:00

  • Terminating histone synthesis to preserve centromere integrity.

    abstract::Histone protein synthesis is activated as cells enter S phase to allow packaging of the newly replicated DNA into chromatin. In this issue of Developmental Cell, Takayama and coworkers elucidate a mechanism for silencing histone expression at the end of S phase in S. pombe. Failure to shut off histone expression disru...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2010.03.004

    authors: Marzluff WF

    更新日期:2010-03-16 00:00:00

  • Crosstalk between a nuclear receptor and beta-catenin signaling decides cell fates in the C. elegans somatic gonad.

    abstract::beta-Catenin signaling determines the proximal-distal axis of the C. elegans gonad by promoting distal fate in asymmetrically dividing somatic gonad precursor cells (SGPs). Impaired function of the Wnt effector POP-1/TCF, its coactivator SYS-1/beta-catenin, and of upstream components including beta-catenin WRM-1 cause...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2006.06.003

    authors: Asahina M,Valenta T,Silhankova M,Korinek V,Jindra M

    更新日期:2006-08-01 00:00:00

  • An enhancer composed of interlocking submodules controls transcriptional autoregulation of suppressor of hairless.

    abstract::Positive autoregulation is an effective mechanism for the long-term maintenance of a transcription factor's expression. This strategy is widely deployed in cell lineages, where the autoregulatory factor controls the activity of a battery of genes that constitute the differentiation program of a postmitotic cell type. ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2014.02.005

    authors: Liu F,Posakony JW

    更新日期:2014-04-14 00:00:00

  • Temporal coordinates: the genes that fix cell fate with birth order.

    abstract::Drosophila neuroblasts sequentially express a set of four transcription factors that specify the fates of their progeny according to the successive order of their generation. In analogy to spatial coordinate genes that specify neuroblasts by position, these sequentially expressed genes can be called "temporal coordina...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/s1534-5807(01)00047-8

    authors: Harris WA

    更新日期:2001-09-01 00:00:00

  • Nuclear Deformation Lets Cells Gauge Their Physical Confinement.

    abstract::How cells sense their physical microenvironment remains incompletely understood. In two recent Science articles, Lomakin et al. (2020) and Venturini et al. (2020) demonstrate that progressive nuclear deformation associated with cellular confinement triggers intracellular events that promote cell contractility and migr...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2021.01.002

    authors: Long JT,Lammerding J

    更新日期:2021-01-25 00:00:00

  • Establishment of Par-Polarized Cortical Domains via Phosphoregulated Membrane Motifs.

    abstract::The Par polarity complex creates mutually exclusive cortical domains in diverse animal cells. Activity of the atypical protein kinase C (aPKC) is a key output of the Par complex as phosphorylation removes substrates from the Par domain. Here, we investigate how diverse, apparently unrelated Par substrates couple phosp...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2015.09.016

    authors: Bailey MJ,Prehoda KE

    更新日期:2015-10-26 00:00:00

  • Wnt signaling regulates postembryonic hypothalamic progenitor differentiation.

    abstract::Previous studies have raised the possibility that Wnt signaling may regulate both neural progenitor maintenance and neuronal differentiation within a single population. Here we investigate the role of Wnt/β-catenin activity in the zebrafish hypothalamus and find that the pathway is first required for the proliferation...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2012.07.012

    authors: Wang X,Kopinke D,Lin J,McPherson AD,Duncan RN,Otsuna H,Moro E,Hoshijima K,Grunwald DJ,Argenton F,Chien CB,Murtaugh LC,Dorsky RI

    更新日期:2012-09-11 00:00:00

  • Functional analysis of chicken Sox2 enhancers highlights an array of diverse regulatory elements that are conserved in mammals.

    abstract::Sox2 expression marks neural and sensory primordia at various stages of development. A 50 kb genomic region of chicken Sox2 was isolated and scanned for enhancer activity utilizing embryo electroporation, resulting in identification of a battery of enhancers. Although Sox2 expression in the early embryonic CNS appears...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/s1534-5807(03)00088-1

    authors: Uchikawa M,Ishida Y,Takemoto T,Kamachi Y,Kondoh H

    更新日期:2003-04-01 00:00:00

  • Drosophila exocyst components Sec5, Sec6, and Sec15 regulate DE-Cadherin trafficking from recycling endosomes to the plasma membrane.

    abstract::The E-Cadherin-catenin complex plays a critical role in epithelial cell-cell adhesion, polarization, and morphogenesis. Here, we have analyzed the mechanism of Drosophila E-Cadherin (DE-Cad) localization. Loss of function of the Drosophila exocyst components sec5, sec6, and sec15 in epithelial cells results in DE-Cad ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2005.07.013

    authors: Langevin J,Morgan MJ,Sibarita JB,Aresta S,Murthy M,Schwarz T,Camonis J,Bellaïche Y

    更新日期:2005-09-01 00:00:00

  • Regulation of plant stem cell quiescence by a brassinosteroid signaling module.

    abstract::The quiescent center (QC) maintains the activity of the surrounding stem cells within the root stem cell niche, yet specific molecular players sustaining the low rate of QC cell division remain poorly understood. Here, we identified a R2R3-MYB transcription factor, BRAVO (BRASSINOSTEROIDS AT VASCULAR AND ORGANIZING CE...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2014.05.020

    authors: Vilarrasa-Blasi J,González-García MP,Frigola D,Fàbregas N,Alexiou KG,López-Bigas N,Rivas S,Jauneau A,Lohmann JU,Benfey PN,Ibañes M,Caño-Delgado AI

    更新日期:2014-07-14 00:00:00

  • Regulation of stem cells by intersecting gradients of long-range niche signals.

    abstract::We have used Drosophila ovarian follicle stem cells (FSCs) to study how stem cells are regulated by external signals and draw three main conclusions. First, the spatial definition of supportive niche positions for FSCs depends on gradients of Hh and JAK-STAT pathway ligands, which emanate from opposite, distant sites....

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2012.09.010

    authors: Vied C,Reilein A,Field NS,Kalderon D

    更新日期:2012-10-16 00:00:00

  • The two TORCs and Akt.

    abstract::The regulatory circuits that control the activities of the two distinct target of rapamycin (TOR) complexes, TORC1 and TORC2, and of Akt have been a focus of intense research in recent years. It has become increasingly evident that these regulatory circuits control some of the most fundamental aspects of metabolism, c...

    journal_title:Developmental cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.devcel.2007.03.020

    authors: Bhaskar PT,Hay N

    更新日期:2007-04-01 00:00:00

  • Flower forms an extracellular code that reveals the fitness of a cell to its neighbors in Drosophila.

    abstract::Cell competition promotes the elimination of weaker cells from a growing population. Here we investigate how cells of Drosophila wing imaginal discs distinguish "winners" from "losers" during cell competition. Using genomic and functional assays, we have identified several factors implicated in the process, including ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2010.05.010

    authors: Rhiner C,López-Gay JM,Soldini D,Casas-Tinto S,Martín FA,Lombardía L,Moreno E

    更新日期:2010-06-15 00:00:00