Abstract:
:Determination of left-right asymmetry in mouse embryos is established by a leftward fluid flow that is generated by clockwise rotation of node cilia. How node cilia achieve stable unidirectional rotation has remained unknown, however. Here we show that brief exposure to the microtubule-stabilizing drug paclitaxel (Taxol) induces randomly directed rotation and changes the ultrastructure of node cilia. In vivo observations and a computer simulation revealed that a regular 9+0 arrangement of doublet microtubules is essential for stable unidirectional rotation of node cilia. The 9+2 motile cilia of the airway, which manifest planar beating, are resistant to Taxol treatment. However, the airway cilia of mice lacking the radial spoke head protein Rsph4a undergo rotational movement instead of planar beating, are prone to microtubule rearrangement, and are sensitive to Taxol. Our results suggest that the absence of radial spokes allows node cilia to rotate unidirectionally but, as a trade-off, renders them ultrastructurally fragile.
journal_name
Dev Celljournal_title
Developmental cellauthors
Shinohara K,Chen D,Nishida T,Misaki K,Yonemura S,Hamada Hdoi
10.1016/j.devcel.2015.10.001subject
Has Abstractpub_date
2015-10-26 00:00:00pages
236-46issue
2eissn
1534-5807issn
1878-1551pii
S1534-5807(15)00628-0journal_volume
35pub_type
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