Absence of Radial Spokes in Mouse Node Cilia Is Required for Rotational Movement but Confers Ultrastructural Instability as a Trade-Off.


:Determination of left-right asymmetry in mouse embryos is established by a leftward fluid flow that is generated by clockwise rotation of node cilia. How node cilia achieve stable unidirectional rotation has remained unknown, however. Here we show that brief exposure to the microtubule-stabilizing drug paclitaxel (Taxol) induces randomly directed rotation and changes the ultrastructure of node cilia. In vivo observations and a computer simulation revealed that a regular 9+0 arrangement of doublet microtubules is essential for stable unidirectional rotation of node cilia. The 9+2 motile cilia of the airway, which manifest planar beating, are resistant to Taxol treatment. However, the airway cilia of mice lacking the radial spoke head protein Rsph4a undergo rotational movement instead of planar beating, are prone to microtubule rearrangement, and are sensitive to Taxol. Our results suggest that the absence of radial spokes allows node cilia to rotate unidirectionally but, as a trade-off, renders them ultrastructurally fragile.


Dev Cell


Developmental cell


Shinohara K,Chen D,Nishida T,Misaki K,Yonemura S,Hamada H




Has Abstract


2015-10-26 00:00:00














  • Putting Your Best Egg Forward.

    abstract::In a recent issue of Nature, Bohnert and Kenyon (2017) describe a signaling pathway that prevents transgenerational inheritance of cytoplasmic protein aggregates. Fertilizing sperm trigger aggregate clearance in the ovum by a microautophagy-like effector mechanism mediated by inter-organelle communication between lyso...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Patil CK,Denic V

    更新日期:2018-01-08 00:00:00

  • The bacterial cytoskeleton: in vivo dynamics of the actin-like protein Mbl of Bacillus subtilis.

    abstract::Mbl is a bacterial actin homolog that controls cell morphogenesis in Bacillus subtilis. A functional GFP-Mbl fusion protein was used to examine the behavior of the helical cables formed by Mbl protein in live B. subtilis cells. The cables undergo dynamic changes during cell cycle progression. They are stable but not r...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Carballido-López R,Errington J

    更新日期:2003-01-01 00:00:00

  • Intron Retained, Transcript Detained: Intron Retention as a Hallmark of the Quiescent Satellite Cell State.

    abstract::Molecular signatures defining quiescence in muscle satellite cells (mSCs) remain enigmatic. In this issue of Developmental Cell, Yue et al. adapted an in vivo fixation approach to isolate dormant mSCs from healthy muscle. Characterizing the transcriptome from these cells, they identified intron retention as a novel ha...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Nakka K,Kovac R,Wong MM,Dilworth FJ

    更新日期:2020-06-22 00:00:00

  • SrGAP2-Dependent Integration of Membrane Geometry and Slit-Robo-Repulsive Cues Regulates Fibroblast Contact Inhibition of Locomotion.

    abstract::Migrating fibroblasts undergo contact inhibition of locomotion (CIL), a process that was discovered five decades ago and still is not fully understood at the molecular level. We identify the Slit2-Robo4-srGAP2 signaling network as a key regulator of CIL in fibroblasts. CIL involves highly dynamic contact protrusions w...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Fritz RD,Menshykau D,Martin K,Reimann A,Pontelli V,Pertz O

    更新日期:2015-10-12 00:00:00

  • Ablation in mice of the mTORC components raptor, rictor, or mLST8 reveals that mTORC2 is required for signaling to Akt-FOXO and PKCalpha, but not S6K1.

    abstract::The mTOR kinase controls cell growth, proliferation, and survival through two distinct multiprotein complexes, mTORC1 and mTORC2. mTOR and mLST8 are in both complexes, while raptor and rictor are part of only mTORC1 and mTORC2, respectively. To investigate mTORC1 and mTORC2 function in vivo, we generated mice deficien...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Guertin DA,Stevens DM,Thoreen CC,Burds AA,Kalaany NY,Moffat J,Brown M,Fitzgerald KJ,Sabatini DM

    更新日期:2006-12-01 00:00:00

  • Spindle position in symmetric cell divisions during epiboly is controlled by opposing and dynamic apicobasal forces.

    abstract::Orientation of cell division is a vital aspect of tissue morphogenesis and growth. Asymmetric divisions generate cell fate diversity and epithelial stratification, whereas symmetric divisions contribute to tissue growth, spreading, and elongation. Here, we describe a mechanism for positioning the spindle in symmetric ...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Woolner S,Papalopulu N

    更新日期:2012-04-17 00:00:00

  • Defining a PARticular pathway of neural tube closure.

    abstract::Mammalian neurulation is completed when the dorsolateral neural folds bend inwards, their tips make adhesive contacts across the midline, and the epithelia remodel to create a closed neural tube. Two recent papers (one by Camerer et al. in this issue of Developmental Cell) demonstrate a vital role for protease-activat...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Copp AJ,Greene ND

    更新日期:2010-01-19 00:00:00

  • Inhibition of endothelial cell apoptosis by netrin-1 during angiogenesis.

    abstract::Netrin-1 was recently proposed to play an important role in embryonic and pathological angiogenesis. However, data reported led to the apparently contradictory conclusions that netrin-1 is either a pro- or an antiangiogenic factor. Here, we reconcile these opposing observations by demonstrating that netrin-1 acts as a...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Castets M,Coissieux MM,Delloye-Bourgeois C,Bernard L,Delcros JG,Bernet A,Laudet V,Mehlen P

    更新日期:2009-04-01 00:00:00

  • Tissue-Scale Mechanical Coupling Reduces Morphogenetic Noise to Ensure Precision during Epithelial Folding.

    abstract::Morphological constancy is universal in developing systems. It is unclear whether precise morphogenesis stems from faithful mechanical interpretation of gene expression patterns. We investigate the formation of the cephalic furrow, an epithelial fold that is precisely positioned with a linear morphology. Fold initiati...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Eritano AS,Bromley CL,Bolea Albero A,Schütz L,Wen FL,Takeda M,Fukaya T,Sami MM,Shibata T,Lemke S,Wang YC

    更新日期:2020-04-20 00:00:00

  • Self-assembly of VPS41 promotes sorting required for biogenesis of the regulated secretory pathway.

    abstract::The regulated release of polypeptides has a central role in physiology, behavior, and development, but the mechanisms responsible for production of the large dense core vesicles (LDCVs) capable of regulated release have remained poorly understood. Recent work has implicated cytosolic adaptor protein AP-3 in the recrui...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Asensio CS,Sirkis DW,Maas JW Jr,Egami K,To TL,Brodsky FM,Shu X,Cheng Y,Edwards RH

    更新日期:2013-11-25 00:00:00

  • EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality.

    abstract::Positioning organs in the body often requires the movement of multiple tissues, yet the molecular and cellular mechanisms coordinating such movements are largely unknown. Here, we show that bidirectional signaling between EphrinB1 and EphB3b coordinates the movements of the hepatic endoderm and adjacent lateral plate ...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Cayuso J,Dzementsei A,Fischer JC,Karemore G,Caviglia S,Bartholdson J,Wright GJ,Ober EA

    更新日期:2016-11-07 00:00:00

  • The Drosophila myosin VI Jaguar is required for basal protein targeting and correct spindle orientation in mitotic neuroblasts.

    abstract::Asymmetric cell divisions generate cellular diversity. In Drosophila, embryonic neuroblasts target cell fate determinants basally, rotate their spindles by 90 degrees to align with the apical-basal axis, and divide asymmetrically in a stem cell-like fashion. In this process, apically localized Bazooka recruits Inscute...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Petritsch C,Tavosanis G,Turck CW,Jan LY,Jan YN

    更新日期:2003-02-01 00:00:00

  • How the sea squirt nucleus tells mesoderm not to be endoderm.

    abstract::Sea squirts are simple invertebrate chordates. In this issue of Developmental Cell, Takatori et al. show nuclear migration within ascidian mesendodermal cells enables polarized localization of Not mRNA, which encodes a homeobox protein that distinguishes mesoderm from endoderm fates. The link between nuclear migration...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Parton RM,Davis I

    更新日期:2010-10-19 00:00:00

  • Coordinating morphogenesis: epithelial integrity during heart tube assembly.

    abstract::Formation of the embryonic heart tube requires the medial migration and merger of bilateral precursor populations. A new study of zebrafish cardiogenesis published in this issue of Developmental Cell indicates that precursor migration involves formation of a coherent epithelium and that fibronectin plays an important ...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Glickman NS,Yelon D

    更新日期:2004-03-01 00:00:00

  • Interplay between the dividing cell and its neighbors regulates adherens junction formation during cytokinesis in epithelial tissue.

    abstract::How adherens junctions (AJs) are formed upon cell division is largely unexplored. Here, we found that AJ formation is coordinated with cytokinesis and relies on an interplay between the dividing cell and its neighbors. During contraction of the cytokinetic ring, the neighboring cells locally accumulate Myosin II and p...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Herszterg S,Leibfried A,Bosveld F,Martin C,Bellaiche Y

    更新日期:2013-02-11 00:00:00

  • Neuronal osmotransduction: push-activating TRPV1 with microtubules.

    abstract::Regulating the osmotic pressure of our body fluids relies on osmosensory neurons that depolarize when their volume decreases. Recently in Neuron, Prager-Khoutorsky et al. (2014) report that this depolarization arises from direct interactions between the transient receptor potential channel TRPV1 and microtubules, whic...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Andrés M,Göpfert MC

    更新日期:2014-08-25 00:00:00

  • Regulation of stem cells by intersecting gradients of long-range niche signals.

    abstract::We have used Drosophila ovarian follicle stem cells (FSCs) to study how stem cells are regulated by external signals and draw three main conclusions. First, the spatial definition of supportive niche positions for FSCs depends on gradients of Hh and JAK-STAT pathway ligands, which emanate from opposite, distant sites....

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Vied C,Reilein A,Field NS,Kalderon D

    更新日期:2012-10-16 00:00:00

  • Sister chromatid cohesion at the centromere: confrontation between kinases and phosphatases?

    abstract::Accurate chromosome segregation in mitosis and meiosis requires that the cohesin complex be protected at the centromere by the Shugoshin/MEI-S332 protein family. Recent studies show that Sgo directly binds the phosphatase PP2A, tethering it to the centromere where it can protect cohesin subunits from phosphorylation, ...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Clarke A,Orr-Weaver TL

    更新日期:2006-05-01 00:00:00

  • Chk1: a double agent in cell cycle checkpoints.

    abstract::Two cell cycle surveillance systems--the DNA damage checkpoint and the spindle checkpoint--guard against genomic instability. The protein kinase Chk1 is a well-established signal transducer in the DNA damage checkpoint. In this issue of Developmental Cell, Zachos et al.(2007) present evidence to indicate that Chk1 als...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Yu H

    更新日期:2007-02-01 00:00:00

  • The CXCL12/CXCR4 Axis Plays a Critical Role in Coronary Artery Development.

    abstract::The chemokine CXCL12 and its receptor CXCR4 have many functions during embryonic and post-natal life. We used murine models to investigate the role of CXCL12/CXCR4 signaling in cardiac development and found that embryonic Cxcl12-null hearts lacked intra-ventricular coronary arteries (CAs) and exhibited absent or mispl...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Ivins S,Chappell J,Vernay B,Suntharalingham J,Martineau A,Mohun TJ,Scambler PJ

    更新日期:2015-05-26 00:00:00

  • A Balance of Yki/Sd Activator and E2F1/Sd Repressor Complexes Controls Cell Survival and Affects Organ Size.

    abstract::The Hippo/Yki and RB/E2F pathways both regulate tissue growth by affecting cell proliferation and survival, but interactions between these parallel control systems are poorly defined. In this study, we demonstrate that interaction between Drosophila E2F1 and Sd disrupts Yki/Sd complex formation and thereby suppresses ...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Zhang P,Pei C,Wang X,Xiang J,Sun BF,Cheng Y,Qi X,Marchetti M,Xu JW,Sun YP,Edgar BA,Yuan Z

    更新日期:2017-12-04 00:00:00

  • Diffusion of nodal signaling activity in the absence of the feedback inhibitor Lefty2.

    abstract::The role of Lefty2 in left-right patterning was investigated by analysis of mutant mice that lack asymmetric expression of lefty2. These animals exhibited various situs defects including left isomerism. The asymmetric expression of nodal was prolonged and the expression of Pitx2 was upregulated in the mutant embryos. ...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Meno C,Takeuchi J,Sakuma R,Koshiba-Takeuchi K,Ohishi S,Saijoh Y,Miyazaki J,ten Dijke P,Ogura T,Hamada H

    更新日期:2001-07-01 00:00:00

  • Regulation of angiogenesis by oxygen and metabolism.

    abstract::Blood vessels form an important interface between the environment and the organism by carrying oxygen and nutrients to all cells and thus determining cellular metabolism. It is therefore not surprising that oxygen and metabolism influence the development of the vascular network. Here, we discuss recent insights regard...

    journal_title:Developmental cell

    pub_type: 杂志文章,评审


    authors: Fraisl P,Mazzone M,Schmidt T,Carmeliet P

    更新日期:2009-02-01 00:00:00

  • A dual role for UVRAG in maintaining chromosomal stability independent of autophagy.

    abstract::Autophagy defects have recently been associated with chromosomal instability, a hallmark of human cancer. However, the functional specificity and mechanism of action of autophagy-related factors in genome stability remain elusive. Here we report that UVRAG, an autophagic tumor suppressor, plays a dual role in chromoso...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Zhao Z,Oh S,Li D,Ni D,Pirooz SD,Lee JH,Yang S,Lee JY,Ghozalli I,Costanzo V,Stark JM,Liang C

    更新日期:2012-05-15 00:00:00

  • Membrane Mechanics in Living Cells.

    abstract::The combination of fluid and mechanical properties exhibited by cell membranes enables a wealth of physical phenomena, many of which also have biological functions. A recent study in Cell (Shi et al., 2018) now shows that membrane tension in the cell may not be as simple as it first seemed. ...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Groves JT

    更新日期:2019-01-07 00:00:00

  • MT1-MMP-dependent control of skeletal stem cell commitment via a β1-integrin/YAP/TAZ signaling axis.

    abstract::In vitro, topographical and biophysical cues arising from the extracellular matrix (ECM) direct skeletal stem cell (SSC) commitment and differentiation. However, the mechanisms by which the SSC-ECM interface is regulated and the outcome of such interactions on stem cell fate in vivo remain unknown. Here we demonstrate...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Tang Y,Rowe RG,Botvinick EL,Kurup A,Putnam AJ,Seiki M,Weaver VM,Keller ET,Goldstein S,Dai J,Begun D,Saunders T,Weiss SJ

    更新日期:2013-05-28 00:00:00

  • An Excitable Ras/PI3K/ERK Signaling Network Controls Migration and Oncogenic Transformation in Epithelial Cells.

    abstract::The Ras/PI3K/extracellular signal-regulated kinases (ERK) signaling network plays fundamental roles in cell growth, survival, and migration and is frequently activated in cancer. Here, we show that the activities of the signaling network propagate as coordinated waves, biased by growth factor, which drive actin-based ...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Zhan H,Bhattacharya S,Cai H,Iglesias PA,Huang CH,Devreotes PN

    更新日期:2020-09-14 00:00:00

  • The Perinuclear ER Scales Nuclear Size Independently of Cell Size in Early Embryos.

    abstract::Nuclear size plays pivotal roles in gene expression, embryo development, and disease. A central hypothesis in organisms ranging from yeast to vertebrates is that nuclear size scales to cell size. This implies that nuclei may reach steady-state sizes set by limiting cytoplasmic pools of size-regulating components. By m...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Mukherjee RN,Sallé J,Dmitrieff S,Nelson KM,Oakey J,Minc N,Levy DL

    更新日期:2020-08-10 00:00:00

  • Arabidopsis Serrate Coordinates Histone Methyltransferases ATXR5/6 and RNA Processing Factor RDR6 to Regulate Transposon Expression.

    abstract::Serrate (SE) is a key component in RNA metabolism. Little is known about whether and how it can regulate epigenetic silencing. Here, we report histone methyltransferases ATXR5 and ATXR6 (ATXR5/6) as novel partners of SE. ATXR5/6 deposit histone 3 lysine 27 monomethylation (H3K27me1) to promote heterochromatin formatio...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Ma Z,Castillo-González C,Wang Z,Sun D,Hu X,Shen X,Potok ME,Zhang X

    更新日期:2018-06-18 00:00:00

  • MicroRNAs Establish the Right-Handed Dominance of the Heart Laterality Pathway in Vertebrates.

    abstract::Despite their external bilateral symmetry, vertebrates have internal left/right (L/R) asymmetries required for optimal organ function. BMP-induced epithelial to mesenchymal transition (EMT) in the lateral plate mesoderm (LPM) triggers L/R asymmetric cell movements toward the midline, higher from the right, which are c...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Rago L,Castroviejo N,Fazilaty H,Garcia-Asencio F,Ocaña OH,Galcerán J,Nieto MA

    更新日期:2019-11-18 00:00:00