Absence of Radial Spokes in Mouse Node Cilia Is Required for Rotational Movement but Confers Ultrastructural Instability as a Trade-Off.


:Determination of left-right asymmetry in mouse embryos is established by a leftward fluid flow that is generated by clockwise rotation of node cilia. How node cilia achieve stable unidirectional rotation has remained unknown, however. Here we show that brief exposure to the microtubule-stabilizing drug paclitaxel (Taxol) induces randomly directed rotation and changes the ultrastructure of node cilia. In vivo observations and a computer simulation revealed that a regular 9+0 arrangement of doublet microtubules is essential for stable unidirectional rotation of node cilia. The 9+2 motile cilia of the airway, which manifest planar beating, are resistant to Taxol treatment. However, the airway cilia of mice lacking the radial spoke head protein Rsph4a undergo rotational movement instead of planar beating, are prone to microtubule rearrangement, and are sensitive to Taxol. Our results suggest that the absence of radial spokes allows node cilia to rotate unidirectionally but, as a trade-off, renders them ultrastructurally fragile.


Dev Cell


Developmental cell


Shinohara K,Chen D,Nishida T,Misaki K,Yonemura S,Hamada H




Has Abstract


2015-10-26 00:00:00














  • A KRAB domain zinc finger protein in imprinting and disease.

    abstract::The monoallelic expression of imprinted genes is regulated by DNA methylation marks that originate from the oocyte or sperm. Li et al. (2008) show in this issue of Developmental Cell that the KRAB zinc finger protein Zfp57 contributes to the embryonic maintenance of these imprints. At one locus, Zfp57 is also involved...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Hirasawa R,Feil R

    更新日期:2008-10-01 00:00:00

  • Displacement of formins from growing barbed ends by bud14 is critical for actin cable architecture and function.

    abstract::Normal cellular development and function require tight spatiotemporal control of actin assembly. Formins are potent actin assembly factors that protect the growing ends of actin filaments from capping proteins. However, it is unresolved how the duration of formin-mediated actin assembly events is controlled, whether f...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Chesarone M,Gould CJ,Moseley JB,Goode BL

    更新日期:2009-02-01 00:00:00

  • Nuclear Deformation Lets Cells Gauge Their Physical Confinement.

    abstract::How cells sense their physical microenvironment remains incompletely understood. In two recent Science articles, Lomakin et al. (2020) and Venturini et al. (2020) demonstrate that progressive nuclear deformation associated with cellular confinement triggers intracellular events that promote cell contractility and migr...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Long JT,Lammerding J

    更新日期:2021-01-25 00:00:00

  • Temporal control of neurogenin3 activity in pancreas progenitors reveals competence windows for the generation of different endocrine cell types.

    abstract::All pancreatic endocrine cells, producing glucagon, insulin, somatostatin, or PP, differentiate from Pdx1+ progenitors that transiently express Neurogenin3. To understand whether the competence of pancreatic progenitors changes over time, we generated transgenic mice expressing a tamoxifen-inducible Ngn3 fusion protei...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Johansson KA,Dursun U,Jordan N,Gu G,Beermann F,Gradwohl G,Grapin-Botton A

    更新日期:2007-03-01 00:00:00

  • An essential role for 14-3-3 proteins in brassinosteroid signal transduction in Arabidopsis.

    abstract::Brassinosteroids (BRs) are essential hormones for plant growth and development. BRs regulate gene expression by inducing dephosphorylation of two key transcription factors, BZR1 and BZR2/BES1, through a signal transduction pathway that involves cell-surface receptors (BRI1 and BAK1) and a GSK3 kinase (BIN2). How BR-re...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Gampala SS,Kim TW,He JX,Tang W,Deng Z,Bai MY,Guan S,Lalonde S,Sun Y,Gendron JM,Chen H,Shibagaki N,Ferl RJ,Ehrhardt D,Chong K,Burlingame AL,Wang ZY

    更新日期:2007-08-01 00:00:00

  • Multiplexing MIM.

    abstract::Signaling circuits often coordinate cellular membranes and actin filaments at distinct sites to direct cell behavior. In this issue of Developmental Cell, Bershteyn et al. outline how the molecular scaffold protein, MIM, which bends membranes and binds actin filaments, is at the middle of one such circuit to regulate ...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Kozminski KG,Schafer DA

    更新日期:2010-08-17 00:00:00

  • The mammalian passenger protein TD-60 is an RCC1 family member with an essential role in prometaphase to metaphase progression.

    abstract::Passenger proteins migrate from inner centromeres to the spindle midzone during late mitosis, and those described to date are essential both for proper chromosome segregation and for completion of cell cleavage. We have purified and cloned the human passenger protein TD-60, and we here report that it is a member of th...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Mollinari C,Reynaud C,Martineau-Thuillier S,Monier S,Kieffer S,Garin J,Andreassen PR,Boulet A,Goud B,Kleman JP,Margolis RL

    更新日期:2003-08-01 00:00:00

  • Epigenetic Compensation Promotes Liver Regeneration.

    abstract::Two major functions of the epigenome are to regulate gene expression and to suppress transposons. It is unclear how these functions are balanced during physiological challenges requiring tissue regeneration, where exquisite coordination of gene expression is essential. Transcriptomic analysis of seven time points foll...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Wang S,Zhang C,Hasson D,Desai A,SenBanerjee S,Magnani E,Ukomadu C,Lujambio A,Bernstein E,Sadler KC

    更新日期:2019-07-01 00:00:00

  • Tgf-beta superfamily signaling in embryonic development and homeostasis.

    abstract::TGF-beta superfamily signaling pathways emerged with the evolution of multicellular animals, suggesting that these pathways contribute to the increased diversity and complexity required for the development and homeostasis of these organisms. In this review we begin by exploring some key developmental and disease proce...

    journal_title:Developmental cell

    pub_type: 杂志文章,评审


    authors: Wu MY,Hill CS

    更新日期:2009-03-01 00:00:00

  • A PLCβ/PI3Kγ-GSK3 signaling pathway regulates cofilin phosphatase slingshot2 and neutrophil polarization and chemotaxis.

    abstract::Neutrophils, in response to a chemoattractant gradient, undergo dynamic F-actin remodeling, a process important for their directional migration or chemotaxis. However, signaling mechanisms for chemoattractants to regulate the process are incompletely understood. Here, we characterized chemoattractant-activated signali...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Tang W,Zhang Y,Xu W,Harden TK,Sondek J,Sun L,Li L,Wu D

    更新日期:2011-12-13 00:00:00

  • Movement of membrane domains and requirement of membrane signaling molecules for cytokinesis.

    abstract::Plasma membrane subdomains enriched in sphingolipids, cholesterol, and signaling proteins are critical for organization of actin, membrane trafficking, and cell polarity, but the role of such domains in cytokinesis in animal cells is unknown. Here, we show that eggs form a plasma membrane domain enriched in gangliosid...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Ng MM,Chang F,Burgess DR

    更新日期:2005-12-01 00:00:00

  • Combining genetic perturbations and proteomics to examine kinase-phosphatase networks in Drosophila embryos.

    abstract::Connecting phosphorylation events to kinases and phosphatases is key to understanding the molecular organization and signaling dynamics of networks. We have generated a validated set of transgenic RNA-interference reagents for knockdown and characterization of all protein kinases and phosphatases present during early ...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Sopko R,Foos M,Vinayagam A,Zhai B,Binari R,Hu Y,Randklev S,Perkins LA,Gygi SP,Perrimon N

    更新日期:2014-10-13 00:00:00

  • Signaling from Glia and Cholinergic Neurons Controls Nutrient-Dependent Production of an Insulin-like Peptide for Drosophila Body Growth.

    abstract::The insulin-like peptide (ILP) family plays key biological roles in the control of body growth. Although the functions of ILPs are well understood, the mechanisms by which organisms sense their nutrient status and thereby control ILP production remain largely unknown. Here, we show that signaling relay and feedback me...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Okamoto N,Nishimura T

    更新日期:2015-11-09 00:00:00

  • Antisocial, an intracellular adaptor protein, is required for myoblast fusion in Drosophila.

    abstract::Somatic muscle formation in Drosophila requires fusion of muscle founder cells with fusion-competent myoblasts. In a genetic screen for genes that control muscle development, we identified antisocial (ants), a gene that encodes an ankyrin repeat-, TPR repeat-, and RING finger-containing protein, required for myoblast ...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Chen EH,Olson EN

    更新日期:2001-11-01 00:00:00

  • Combined deficiency of Tet1 and Tet2 causes epigenetic abnormalities but is compatible with postnatal development.

    abstract::Tet enzymes (Tet1/2/3) convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) in various embryonic and adult tissues. Mice mutant for either Tet1 or Tet2 are viable, raising the question of whether these enzymes have overlapping roles in development. Here we have generated Tet1 and Tet2 double-knockout (DKO)...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Dawlaty MM,Breiling A,Le T,Raddatz G,Barrasa MI,Cheng AW,Gao Q,Powell BE,Li Z,Xu M,Faull KF,Lyko F,Jaenisch R

    更新日期:2013-02-11 00:00:00

  • DjPiwiB: A Rich Nuclear Inheritance for Descendants of Planarian Stem Cells.

    abstract::Stem cell differentiation involves a delicate balance of gene expression and transposon repression. In this issue of Developmental Cell, Shibata et al. (2016) show that a PIWI protein expressed in planarian stem cells is inherited by their differentiating descendants to ensure regenerative capacity of the flatworm via...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Tharp ME,Bortvin A

    更新日期:2016-05-09 00:00:00

  • WRAMPing up calcium in migrating cells by localized ER transport.

    abstract::Morphological plasticity and front-rear polarity are essential for directed cell migration. In this issue of Developmental Cell, Witze et al. (2013) demonstrate that Wnt5a-mediated signaling induces localization of the cortical endoplasmic reticulum to the trailing edge of melanoma cells and mediates calcium flux, rea...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Mladinich KM,Huttenlocher A

    更新日期:2013-09-30 00:00:00

  • Ectodermal Smad4 and p38 MAPK are functionally redundant in mediating TGF-beta/BMP signaling during tooth and palate development.

    abstract::Smad4 is a central intracellular effector of TGF-beta signaling. Smad-independent TGF-beta pathways, such as those mediated by p38 MAPK, have been identified in cell culture systems, but their in vivo functional mechanisms remain unclear. In this study, we investigated the role of TGF-beta signaling in tooth and palat...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Xu X,Han J,Ito Y,Bringas P Jr,Deng C,Chai Y

    更新日期:2008-08-01 00:00:00

  • Picket and other fences in biological membranes.

    abstract::Nakada et al. revisit the controversial question of whether membrane lipids are able to diffuse from the axon of a neuron into the soma. Using single molecule imaging of a fluorescent phospholipid, the authors show that a diffusion barrier in the axon initial segment blocks the diffusion of lipids. ...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Boiko T,Winckler B

    更新日期:2003-08-01 00:00:00

  • Asymmetric cell divisions: a view from plant development.

    abstract::All complex multicellular organisms must solve the problem of generating diverse and appropriately patterned cell types. Asymmetric division, in which a single mother cell gives rise to daughters with distinct identities, is instrumental in the generation of cellular diversity and higher-level patterns. In animal syst...

    journal_title:Developmental cell

    pub_type: 杂志文章,评审


    authors: Abrash EB,Bergmann DC

    更新日期:2009-06-01 00:00:00

  • Drosophila rasiRNA pathway mutations disrupt embryonic axis specification through activation of an ATR/Chk2 DNA damage response.

    abstract::Small repeat-associated siRNAs (rasiRNAs) mediate silencing of retrotransposons and the Stellate locus. Mutations in the Drosophila rasiRNA pathway genes armitage and aubergine disrupt embryonic axis specification, triggering defects in microtubule polarization as well as asymmetric localization of mRNA and protein de...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Klattenhoff C,Bratu DP,McGinnis-Schultz N,Koppetsch BS,Cook HA,Theurkauf WE

    更新日期:2007-01-01 00:00:00

  • Symplastic intercellular connectivity regulates lateral root patterning.

    abstract::Cell-to-cell communication coordinates the behavior of individual cells to establish organ patterning and development. Although mobile signals are known to be important in lateral root development, the role of plasmodesmata (PD)-mediated transport in this process has not been investigated. Here, we show that changes i...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Benitez-Alfonso Y,Faulkner C,Pendle A,Miyashima S,Helariutta Y,Maule A

    更新日期:2013-07-29 00:00:00

  • The cytokinin-activated transcription factor ARR2 promotes plant immunity via TGA3/NPR1-dependent salicylic acid signaling in Arabidopsis.

    abstract::Cytokinins affect plant immunity to various pathogens; however, the mechanisms coupling plant-derived cytokinins to pathogen responses have been elusive. Here, we found that plant-derived cytokinins promote resistance of Arabidopsis to Pseudomonas syringae pv. tomato DC3000 (Pst). Modulated cytokinin levels or signali...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Choi J,Huh SU,Kojima M,Sakakibara H,Paek KH,Hwang I

    更新日期:2010-08-17 00:00:00

  • Six2 and Wnt regulate self-renewal and commitment of nephron progenitors through shared gene regulatory networks.

    abstract::A balance between Six2-dependent self-renewal and canonical Wnt signaling-directed commitment regulates mammalian nephrogenesis. Intersectional studies using chromatin immunoprecipitation and transcriptional profiling identified direct target genes shared by each pathway within nephron progenitors. Wnt4 and Fgf8 are e...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Park JS,Ma W,O'Brien LL,Chung E,Guo JJ,Cheng JG,Valerius MT,McMahon JA,Wong WH,McMahon AP

    更新日期:2012-09-11 00:00:00

  • Antagonistic Activities of Sox2 and Brachyury Control the Fate Choice of Neuro-Mesodermal Progenitors.

    abstract::The spinal cord and mesodermal tissues of the trunk such as the vertebral column and skeletal musculature derive from neuro-mesodermal progenitors (NMPs). Sox2, Brachyury (T), and Tbx6 have been correlated with NMP potency and lineage choice; however, their exact role and interaction in these processes have not yet be...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Koch F,Scholze M,Wittler L,Schifferl D,Sudheer S,Grote P,Timmermann B,Macura K,Herrmann BG

    更新日期:2017-09-11 00:00:00

  • Mechanochemical Polarization of Contiguous Cell Walls Shapes Plant Pavement Cells.

    abstract::The epidermis of aerial plant organs is thought to be limiting for growth, because it acts as a continuous load-bearing layer, resisting tension. Leaf epidermis contains jigsaw puzzle piece-shaped pavement cells whose shape has been proposed to be a result of subcellular variations in expansion rate that induce local ...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Majda M,Grones P,Sintorn IM,Vain T,Milani P,Krupinski P,Zagórska-Marek B,Viotti C,Jönsson H,Mellerowicz EJ,Hamant O,Robert S

    更新日期:2017-11-06 00:00:00

  • Rab6 regulates transport and targeting of exocytotic carriers.

    abstract::Constitutive exocytosis delivers newly synthesized proteins, lipids, and other molecules from the Golgi apparatus to the cell surface. This process is mediated by vesicles, which bud off the trans-Golgi network, move along cytoskeletal filaments, and fuse with the plasma membrane. Here, we show that the small GTPase R...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Grigoriev I,Splinter D,Keijzer N,Wulf PS,Demmers J,Ohtsuka T,Modesti M,Maly IV,Grosveld F,Hoogenraad CC,Akhmanova A

    更新日期:2007-08-01 00:00:00

  • Foxf2 Is Required for Brain Pericyte Differentiation and Development and Maintenance of the Blood-Brain Barrier.

    abstract::Pericytes are critical for cerebrovascular maturation and development of the blood-brain barrier (BBB), but their role in maintenance of the adult BBB, and how CNS pericytes differ from those of other tissues, is less well understood. We show that the forkhead transcription factor Foxf2 is specifically expressed in pe...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Reyahi A,Nik AM,Ghiami M,Gritli-Linde A,Pontén F,Johansson BR,Carlsson P

    更新日期:2015-07-06 00:00:00

  • Live Visualization of ERK Activity in the Mouse Blastocyst Reveals Lineage-Specific Signaling Dynamics.

    abstract::FGF/ERK signaling is crucial for the patterning and proliferation of cell lineages that comprise the mouse blastocyst. However, ERK signaling dynamics have never been directly visualized in live embryos. To address whether differential signaling is associated with particular cell fates and states, we generated a targe...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Simon CS,Rahman S,Raina D,Schröter C,Hadjantonakis AK

    更新日期:2020-11-09 00:00:00

  • The transcriptional control of trunk neural crest induction, survival, and delamination.

    abstract::Trunk neural crest cells are generated at the border between the neural plate and nonneural ectoderm, where they initiate a distinct program of gene expression, undergo an epithelial-mesenchymal transition (EMT), and delaminate from the neuroepithelium. Here, we provide evidence that members of three families of trans...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Cheung M,Chaboissier MC,Mynett A,Hirst E,Schedl A,Briscoe J

    更新日期:2005-02-01 00:00:00