Absence of Radial Spokes in Mouse Node Cilia Is Required for Rotational Movement but Confers Ultrastructural Instability as a Trade-Off.

Abstract:

:Determination of left-right asymmetry in mouse embryos is established by a leftward fluid flow that is generated by clockwise rotation of node cilia. How node cilia achieve stable unidirectional rotation has remained unknown, however. Here we show that brief exposure to the microtubule-stabilizing drug paclitaxel (Taxol) induces randomly directed rotation and changes the ultrastructure of node cilia. In vivo observations and a computer simulation revealed that a regular 9+0 arrangement of doublet microtubules is essential for stable unidirectional rotation of node cilia. The 9+2 motile cilia of the airway, which manifest planar beating, are resistant to Taxol treatment. However, the airway cilia of mice lacking the radial spoke head protein Rsph4a undergo rotational movement instead of planar beating, are prone to microtubule rearrangement, and are sensitive to Taxol. Our results suggest that the absence of radial spokes allows node cilia to rotate unidirectionally but, as a trade-off, renders them ultrastructurally fragile.

journal_name

Dev Cell

journal_title

Developmental cell

authors

Shinohara K,Chen D,Nishida T,Misaki K,Yonemura S,Hamada H

doi

10.1016/j.devcel.2015.10.001

subject

Has Abstract

pub_date

2015-10-26 00:00:00

pages

236-46

issue

2

eissn

1534-5807

issn

1878-1551

pii

S1534-5807(15)00628-0

journal_volume

35

pub_type

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