Biosynthesis of the mycotoxin tenuazonic acid by a fungal NRPS-PKS hybrid enzyme.

Abstract:

:Tenuazonic acid (TeA) is a well-known mycotoxin produced by various plant pathogenic fungi. However, its biosynthetic gene has been unknown to date. Here we identify the TeA biosynthetic gene from Magnaporthe oryzae by finding two TeA-inducing conditions of a low-producing strain. We demonstrate that TeA is synthesized from isoleucine and acetoacetyl-coenzyme A by TeA synthetase 1 (TAS1). TAS1 is a unique non-ribosomal peptide synthetase and polyketide synthase (NRPS-PKS) hybrid enzyme that begins with an NRPS module. In contrast to other NRPS/PKS hybrid enzymes, the PKS portion of TAS1 has only a ketosynthase (KS) domain and this domain is indispensable for TAS1 activity. Phylogenetic analysis classifies this KS domain as an independent clade close to type I PKS KS domain. We demonstrate that the TAS1 KS domain conducts the final cyclization step for TeA release. These results indicate that TAS1 is a unique type of NRPS-PKS hybrid enzyme.

journal_name

Nat Commun

journal_title

Nature communications

authors

Yun CS,Motoyama T,Osada H

doi

10.1038/ncomms9758

subject

Has Abstract

pub_date

2015-10-27 00:00:00

pages

8758

issn

2041-1723

pii

ncomms9758

journal_volume

6

pub_type

杂志文章
  • Cell-permeable succinate prodrugs bypass mitochondrial complex I deficiency.

    abstract::Mitochondrial complex I (CI) deficiency is the most prevalent defect in the respiratory chain in paediatric mitochondrial disease. This heterogeneous group of diseases includes serious or fatal neurological presentations such as Leigh syndrome and there are very limited evidence-based treatment options available. Here...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms12317

    authors: Ehinger JK,Piel S,Ford R,Karlsson M,Sjövall F,Frostner EÅ,Morota S,Taylor RW,Turnbull DM,Cornell C,Moss SJ,Metzsch C,Hansson MJ,Fliri H,Elmér E

    更新日期:2016-08-09 00:00:00

  • Observation of an acoustic octupole topological insulator.

    abstract::Berry phase associated with energy bands in crystals can lead to quantised observables like quantised dipole polarizations in one-dimensional topological insulators. Recent theories have generalised the concept of quantised dipoles to multipoles, resulting in the discovery of multipole topological insulators which exh...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-16350-1

    authors: Xue H,Ge Y,Sun HX,Wang Q,Jia D,Guan YJ,Yuan SQ,Chong Y,Zhang B

    更新日期:2020-05-15 00:00:00

  • Bio-optimized energy transfer in densely packed fluorescent protein enables near-maximal luminescence and solid-state lasers.

    abstract::Bioluminescent organisms are likely to have an evolutionary drive towards high radiance. As such, bio-optimized materials derived from them hold great promise for photonic applications. Here, we show that biologically produced fluorescent proteins retain their high brightness even at the maximum density in solid state...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms6722

    authors: Gather MC,Yun SH

    更新日期:2014-12-08 00:00:00

  • Environment-responsive nanophores for therapy and treatment monitoring via molecular MRI quenching.

    abstract::The effective delivery of therapeutics to disease sites significantly contributes to drug efficacy, toxicity and clearance. Here we demonstrate that clinically approved iron oxide nanoparticles (Ferumoxytol) can be utilized to carry one or multiple drugs. These so called 'nanophores' retain their cargo within their po...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms4384

    authors: Kaittanis C,Shaffer TM,Ogirala A,Santra S,Perez JM,Chiosis G,Li Y,Josephson L,Grimm J

    更新日期:2014-03-04 00:00:00

  • Metformin induces lipogenic differentiation in myofibroblasts to reverse lung fibrosis.

    abstract::Idiopathic pulmonary fibrosis (IPF) is a fatal disease in which the intricate alveolar network of the lung is progressively replaced by fibrotic scars. Myofibroblasts are the effector cells that excessively deposit extracellular matrix proteins thus compromising lung structure and function. Emerging literature suggest...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-10839-0

    authors: Kheirollahi V,Wasnick RM,Biasin V,Vazquez-Armendariz AI,Chu X,Moiseenko A,Weiss A,Wilhelm J,Zhang JS,Kwapiszewska G,Herold S,Schermuly RT,Mari B,Li X,Seeger W,Günther A,Bellusci S,El Agha E

    更新日期:2019-07-05 00:00:00

  • The staphylococcal toxins γ-haemolysin AB and CB differentially target phagocytes by employing specific chemokine receptors.

    abstract::Evasion of the host phagocyte response by Staphylococcus aureus is crucial to successful infection with the pathogen. γ-haemolysin AB and CB (HlgAB, HlgCB) are bicomponent pore-forming toxins present in almost all human S. aureus isolates. Cellular tropism and contribution of the toxins to S. aureus pathophysiology ar...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms6438

    authors: Spaan AN,Vrieling M,Wallet P,Badiou C,Reyes-Robles T,Ohneck EA,Benito Y,de Haas CJ,Day CJ,Jennings MP,Lina G,Vandenesch F,van Kessel KP,Torres VJ,van Strijp JA,Henry T

    更新日期:2014-11-11 00:00:00

  • Excitonic effects from geometric order and disorder explain broadband optical absorption in eumelanin.

    abstract::Eumelanin is a ubiquitous biological pigment, and the origin of its broadband absorption spectrum has long been a topic of scientific debate. Here, we report a first-principles computational investigation to explain its broadband absorption feature. These computations are complemented by experimental results showing a...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms4859

    authors: Chen CT,Chuang C,Cao J,Ball V,Ruch D,Buehler MJ

    更新日期:2014-05-22 00:00:00

  • Balancing mcr-1 expression and bacterial survival is a delicate equilibrium between essential cellular defence mechanisms.

    abstract::MCR-1 is a lipid A modifying enzyme that confers resistance to the antibiotic colistin. Here, we analyse the impact of MCR-1 expression on E. coli morphology, fitness, competitiveness, immune stimulation and virulence. Increased expression of mcr-1 results in decreased growth rate, cell viability, competitive ability ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-017-02149-0

    authors: Yang Q,Li M,Spiller OB,Andrey DO,Hinchliffe P,Li H,MacLean C,Niumsup P,Powell L,Pritchard M,Papkou A,Shen Y,Portal E,Sands K,Spencer J,Tansawai U,Thomas D,Wang S,Wang Y,Shen J,Walsh T

    更新日期:2017-12-12 00:00:00

  • High precision epidermal radio frequency antenna via nanofiber network for wireless stretchable multifunction electronics.

    abstract::Recently, stretchable electronics combined with wireless technology have been crucial for realizing efficient human-machine interaction. Here, we demonstrate highly stretchable transparent wireless electronics composed of Ag nanofibers coils and functional electronic components for power transfer and information commu...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-19367-8

    authors: Zhang Y,Huo Z,Wang X,Han X,Wu W,Wan B,Wang H,Zhai J,Tao J,Pan C,Wang ZL

    更新日期:2020-11-06 00:00:00

  • The higher order auditory cortex is involved in the assignment of affective value to sensory stimuli.

    abstract::The sensory cortex participates in emotional memory but its role is poorly understood. Here we show that inactivation of the higher order auditory cortex Te2 in rats during early memory consolidation impairs remote first- and second-order fear memories but not the association between two neutral cues. Furthermore, Te2...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms9886

    authors: Grosso A,Cambiaghi M,Renna A,Milano L,Roberto Merlo G,Sacco T,Sacchetti B

    更新日期:2015-12-01 00:00:00

  • A rapid cosmic-ray increase in BC 3372-3371 from ancient buried tree rings in China.

    abstract::Cosmic rays interact with the Earth's atmosphere to produce 14C, which can be absorbed by trees. Therefore, rapid increases of 14C in tree rings can be used to probe previous cosmic-ray events. By this method, three 14C rapidly increasing events have been found. Plausible causes of these events include large solar pro...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-017-01698-8

    authors: Wang FY,Yu H,Zou YC,Dai ZG,Cheng KS

    更新日期:2017-11-14 00:00:00

  • Real-time observations of TRIP-induced ultrahigh strain hardening in a dual-phase CrMnFeCoNi high-entropy alloy.

    abstract::Strategies involving metastable phases have been the basis of the design of numerous alloys, yet research on metastable high-entropy alloys is still in its infancy. In dual-phase high-entropy alloys, the combination of local chemical environments and loading-induced crystal structure changes suggests a relationship be...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-14641-1

    authors: Chen S,Oh HS,Gludovatz B,Kim SJ,Park ES,Zhang Z,Ritchie RO,Yu Q

    更新日期:2020-02-11 00:00:00

  • The cryo-electron microscopy supramolecular structure of the bacterial stressosome unveils its mechanism of activation.

    abstract::How the stressosome, the epicenter of the stress response in bacteria, transmits stress signals from the environment has remained elusive. The stressosome consists of multiple copies of three proteins RsbR, RsbS and RsbT, a kinase that is important for its activation. Using cryo-electron microscopy, we determined the ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-10782-0

    authors: Williams AH,Redzej A,Rolhion N,Costa TRD,Rifflet A,Waksman G,Cossart P

    更新日期:2019-07-08 00:00:00

  • Highly explosive basaltic eruptions driven by CO2 exsolution.

    abstract::The most explosive basaltic scoria cone eruption yet documented (>20 km high plumes) occurred at Sunset Crater (Arizona) ca. 1085 AD by undetermined eruptive mechanisms. We present melt inclusion analysis, including bubble contents by Raman spectroscopy, yielding high total CO2 (approaching 6000 ppm) and S (~2000 ppm)...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-20354-2

    authors: Allison CM,Roggensack K,Clarke AB

    更新日期:2021-01-11 00:00:00

  • Pin1-dependent signalling negatively affects GABAergic transmission by modulating neuroligin2/gephyrin interaction.

    abstract::The cell adhesion molecule Neuroligin2 (NL2) is localized selectively at GABAergic synapses, where it interacts with the scaffolding protein gephyrin in the post-synaptic density. However, the role of this interaction for formation and plasticity of GABAergic synapses is unclear. Here, we demonstrate that endogenous N...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms6066

    authors: Antonelli R,Pizzarelli R,Pedroni A,Fritschy JM,Del Sal G,Cherubini E,Zacchi P

    更新日期:2014-10-09 00:00:00

  • Large lattice distortions and size-dependent bandgap modulation in epitaxial halide perovskite nanowires.

    abstract::Metal-halide perovskites have been shown to be remarkable and promising optoelectronic materials. However, despite ongoing research from multiple perspectives, some fundamental questions regarding their optoelectronic properties remain controversial. One reason is the high-variance of data collected from, often unstab...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-14365-2

    authors: Oksenberg E,Merdasa A,Houben L,Kaplan-Ashiri I,Rothman A,Scheblykin IG,Unger EL,Joselevich E

    更新日期:2020-01-24 00:00:00

  • MicroRNA-mRNA interactions underlying colorectal cancer molecular subtypes.

    abstract::Colorectal cancer (CRC) transcriptional subtypes have been recently identified by gene expression profiling. Here we describe an analytical pipeline, microRNA master regulator analysis (MMRA), developed to search for microRNAs potentially driving CRC subtypes. Starting from a microRNA-mRNA tumour expression data set, ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms9878

    authors: Cantini L,Isella C,Petti C,Picco G,Chiola S,Ficarra E,Caselle M,Medico E

    更新日期:2015-11-17 00:00:00

  • Human occupation of northern India spans the Toba super-eruption ~74,000 years ago.

    abstract::India is located at a critical geographic crossroads for understanding the dispersal of Homo sapiens out of Africa and into Asia and Oceania. Here we report evidence for long-term human occupation, spanning the last ~80 thousand years, at the site of Dhaba in the Middle Son River Valley of Central India. An unchanging...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-14668-4

    authors: Clarkson C,Harris C,Li B,Neudorf CM,Roberts RG,Lane C,Norman K,Pal J,Jones S,Shipton C,Koshy J,Gupta MC,Mishra DP,Dubey AK,Boivin N,Petraglia M

    更新日期:2020-02-25 00:00:00

  • Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.

    abstract::A short half-life in the circulation limits the application of therapeutics such as single-domain antibodies (VHHs). We utilize red blood cells to prolong the circulatory half-life of VHHs. Here we present VHHs against botulinum neurotoxin A (BoNT/A) on the surface of red blood cells by expressing chimeric proteins of...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-017-00448-0

    authors: Huang NJ,Pishesha N,Mukherjee J,Zhang S,Deshycka R,Sudaryo V,Dong M,Shoemaker CB,Lodish HF

    更新日期:2017-09-04 00:00:00

  • A unified strategy for the synthesis of highly oxygenated diaryl ethers featured in ellagitannins.

    abstract::Ellagitannins are a family of polyphenols containing more than 1,000 natural products. Nearly 40% of these compounds contain a highly oxygenated diaryl ether that is one of the most critical elements to their structural diversity. Here, we report a unified strategy for the synthesis of highly oxygenated diaryl ethers ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms4478

    authors: Hirokane T,Hirata Y,Ishimoto T,Nishii K,Yamada H

    更新日期:2014-03-24 00:00:00

  • Direct imaging of structural disordering and heterogeneous dynamics of fullerene molecular liquid.

    abstract::Structural rearrangements govern the various properties of disordered systems and visualization of these dynamical processes can provide critical information on structural deformation and phase transformation of the systems. However, direct imaging of individual atoms or molecules in a disordered state is quite challe...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-12320-4

    authors: Choe J,Lee Y,Park J,Kim Y,Kim CU,Kim K

    更新日期:2019-09-27 00:00:00

  • The origin and adaptive evolution of domesticated populations of yeast from Far East Asia.

    abstract::The yeast Saccharomyces cerevisiae has been an essential component of human civilization because of its long global history of use in food and beverage fermentation. However, the diversity and evolutionary history of the domesticated populations of the yeast remain elusive. We show here that China/Far East Asia is lik...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-05106-7

    authors: Duan SF,Han PJ,Wang QM,Liu WQ,Shi JY,Li K,Zhang XL,Bai FY

    更新日期:2018-07-12 00:00:00

  • Enhanced Moran effect by spatial variation in environmental autocorrelation.

    abstract::Spatial correlations in environmental stochasticity can synchronize populations over wide areas, a phenomenon known as the Moran effect. The Moran effect has been confirmed in field, laboratory and theoretical investigations. Little is known, however, about the Moran effect in a common ecological case, when environmen...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms6993

    authors: Massie TM,Weithoff G,Kuckländer N,Gaedke U,Blasius B

    更新日期:2015-01-09 00:00:00

  • Nuclear membrane protein Lem2 regulates nuclear size through membrane flow.

    abstract::The size of the membrane-bound nucleus scales with cell size in a wide range of cell types but the mechanisms determining overall nuclear size remain largely unknown. Here we investigate the role of fission yeast inner nuclear membrane proteins in determining nuclear size, and propose that the Lap2-Emerin-Man1 domain ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-09623-x

    authors: Kume K,Cantwell H,Burrell A,Nurse P

    更新日期:2019-04-23 00:00:00

  • Microscopic mechanism of biphasic interface relaxation in lithium iron phosphate after delithiation.

    abstract::Charge/discharge of lithium-ion battery cathode material LiFePO4 is mediated by the structure and properties of the interface between delithiated and lithiated phases. Direct observations of the interface in a partially delithiated single crystal as a function of time using scanning transmission electron microscopy an...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-05241-1

    authors: Kobayashi S,Kuwabara A,Fisher CAJ,Ukyo Y,Ikuhara Y

    更新日期:2018-07-20 00:00:00

  • MiR-497∼195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch and HIF-1α activity.

    abstract::A specific bone vessel subtype, strongly positive for CD31 and endomucin (CD31hiEmcnhi), is identified as coupling angiogenesis and osteogenesis. The abundance of type CD31hiEmcnhi vessels decrease during ageing. Here we show that expression of the miR-497∼195 cluster is high in CD31hiEmcnhi endothelium but gradually ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms16003

    authors: Yang M,Li CJ,Sun X,Guo Q,Xiao Y,Su T,Tu ML,Peng H,Lu Q,Liu Q,He HB,Jiang TJ,Lei MX,Wan M,Cao X,Luo XH

    更新日期:2017-07-07 00:00:00

  • Broadband on-chip single-photon spectrometer.

    abstract::Single-photon counters are single-pixel binary devices that click upon the absorption of a photon but obscure its spectral information, whereas resolving the color of detected photons has been in critical demand for frontier astronomical observation, spectroscopic imaging and wavelength division multiplexed quantum co...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-12149-x

    authors: Cheng R,Zou CL,Guo X,Wang S,Han X,Tang HX

    更新日期:2019-09-10 00:00:00

  • Author Correction: Ultrafast bridge planarization in donor-π-acceptor copolymers drives intramolecular charge transfer.

    abstract::The original version of this Article omitted the following from the Acknowledgements:"Satish Patil thanks Department of Science and Technology, New Delhi, India for a Swarnajayanti fellowship."This has now been corrected in both the PDF and HTML versions of the Article. ...

    journal_title:Nature communications

    pub_type: 杂志文章,已发布勘误

    doi:10.1038/s41467-017-02574-1

    authors: Roy P,Jha A,Yasarapudi VB,Ram T,Puttaraju B,Patil S,Dasgupta J

    更新日期:2018-01-03 00:00:00

  • Pax3 cooperates with Ldb1 to direct local chromosome architecture during myogenic lineage specification.

    abstract::Chromatin looping allows enhancer-bound regulatory factors to influence transcription. Large domains, referred to as topologically associated domains, participate in genome organization. However, the mechanisms underlining interactions within these domains, which control gene expression, are not fully understood. Here...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-10318-6

    authors: Magli A,Baik J,Pota P,Cordero CO,Kwak IY,Garry DJ,Love PE,Dynlacht BD,Perlingeiro RCR

    更新日期:2019-05-24 00:00:00

  • A mechanosensory receptor required for food texture detection in Drosophila.

    abstract::Textural properties provide information on the ingestibility, digestibility and state of ripeness or decay of sources of nutrition. Compared with our understanding of the chemosensory assessment of food, little is known about the mechanisms of texture detection. Here we show that Drosophila melanogaster can discrimina...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms14192

    authors: Sánchez-Alcañiz JA,Zappia G,Marion-Poll F,Benton R

    更新日期:2017-01-27 00:00:00