MicroRNA‑200a suppresses epithelial‑to‑mesenchymal transition in rat hepatic stellate cells via GLI family zinc finger 2.


:Hepatic stellate cells (HSCs) have an important role in liver fibrosis. Epithelial‑to‑mesenchymal transition (EMT), which is promoted by the Hedgehog (Hh) signaling pathway, is involved in the activation of HSCs. MicroRNAs (miRNAs/miRs) have been reported to be involved in the progression of liver fibrosis. A previous study indicated that the activation of HSCs was suppressed by miR‑200a via targeting transforming growth factor‑β2 and β‑catenin. However, whether miR‑200a is able to regulate the EMT in HSCs has remained elusive. The present study revealed that miR‑200a was decreased in vitro and in vivo during liver fibrosis. Furthermore, miR‑200a overexpression resulted in the inhibition of proliferation, α‑SMA expression and extracellular matrix production of activated HSCs. Of note, miR‑200a overexpression reduced myofibroblastic markers, including α‑SMA, type I collagen and desmin, and increased the epithelial cell marker E‑cadherin. These results were further confirmed by immunofluorescence staining. Further study showed that the expression of genes associated with Hh signaling, including Hhip, Shh and Gli1, were not affected by miR‑200a. However, Gli2, a downstream signaling protein of the Hh pathway, was inhibited by miR‑200a and confirmed as a target of miR‑200a using a dual luciferase reporter assay. In addition, the inhibition of the Hh pathway by miR‑200a resulted in an increase of BMP‑7 and Id2 as well as a reduction of Snai1 and S100A4. Collectively, the results of the present study demonstrated that miR‑200a suppressed the EMT process in HSCs, at least in part, via Gli2.


Mol Med Rep


Yu F,Zheng Y,Hong W,Chen B,Dong P,Zheng J




Has Abstract


2015-12-01 00:00:00












  • Evaluation of a DNA vaccine encoding Brucella BvrR in BALB/c mice.

    abstract::Brucellosis is an important neglected zoonotic disease, and the pathogens responsible are Brucellae. In order to evaluate the immunogenicity and protective efficacy of a DNA vaccine encoding Brucella BvrR, the recombinant plasmid pCDNA‑BvrR was constructed by inserting the BvrR gene fragment into a pCDNA3.0 vector. Th...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Chen B,Liu B,Zhao Z,Wang G

    更新日期:2019-02-01 00:00:00

  • Evidence for the contribution of genetic variations in regulator of G protein signaling 9 to the genetic susceptibility of heroin dependence.

    abstract::Regulator of G protein signaling (RGS) proteins are responsible for the rapid turnoff of G protein-coupled receptor signaling pathways. RGS9‑2, a brain‑specific splice variant of the RGS9 gene, is highly expressed in the striatum but lowly expressed in the periaqueductal gray and spinal cord, which mediate various act...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Zhu Y,Zhang H

    更新日期:2015-05-01 00:00:00

  • Scavenging of blood glutamate for enhancing brain-to-blood glutamate efflux.

    abstract::The presence of excess glutamate in the brain interstitial fluid characterizes several acute pathological conditions of the brain, including traumatic brain injury and stroke. It has been demonstrated that it is possible to eliminate excess glutamate in the brain by decreasing blood glutamate levels and, accordingly, ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Li Y,Hou X,Qi Q,Wang L,Luo L,Yang S,Zhang Y,Miao Z,Zhang Y,Wang F,Wang H,Huang W,Wang Z,Shen Y,Wang Y

    更新日期:2014-01-01 00:00:00

  • Grape seed proanthocyanidins protect against streptozotocin‑induced diabetic nephropathy by attenuating endoplasmic reticulum stress‑induced apoptosis.

    abstract::Diabetic nephropathy (DN) is by far the most common cause of end‑stage renal disease (ESRD) in industrial countries, accounting for ~45% of all new ESRD cases in the United States. Grape seed proanthocyanidin extracts (GSPE) are powerful antioxidants, with an antioxidant ability 50‑fold greater than that of vitamin E ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Gao Z,Liu G,Hu Z,Shi W,Chen B,Zou P,Li X

    更新日期:2018-08-01 00:00:00

  • Aberrant expression of miR‑130a‑3p in ankylosing spondylitis and its role in regulating T‑cell survival.

    abstract::Ankylosing spondylitis (AS) is a chronic inflammatory rheumatic disease. MicroRNAs (miRNAs) are a group of endogenous small non‑coding RNAs that regulate target genes, and play a critical role in many biological processes. However, the underlying mechanism of specific miRNA, miR‑130a‑3p, in AS remains largely unknown....

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Li F,Si D,Guo X,Guo N,Li D,Zhang L,Jian X,Ma J

    更新日期:2019-10-01 00:00:00

  • Munc13‑4 mediates human neutrophil elastase‑induced airway mucin5AC hypersecretion by interacting with syntaxin2.

    abstract::The overexpression and hypersecretion of mucus is a hallmark of chronic pulmonary inflammatory disease. Mucin5AC (MUC5AC) is a major component of airway gel‑forming mucin. Members of the Unc13 (Munc13) protein family act as important activators of granule exocytosis from various types of mammalian cells. The present s...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Xu R,Zhou J,Zhou XD,Li Q,Perelman JM,Kolosov VP

    更新日期:2018-07-01 00:00:00

  • Dexmedetomidine pretreatment attenuates propofol‑induced neurotoxicity in neuronal cultures from the rat hippocampus.

    abstract::Propofol is widely used for the induction and maintenance of pediatric anesthesia. Previous studies have indicated that propofol can induce apoptosis, and damage cognitive and memory functions. Dexmedetomidine is a potent α‑2 adrenoceptor agonist with high selectivity. Previous observations have shown that dexmedetomi...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Wei Y,Hu J,Liang Y,Zhong Y,He D,Qin Y,Li L,Chen J,Xiao Q,Xie Y

    更新日期:2016-10-01 00:00:00

  • Inhibition of autophagy by berberine enhances the survival of H9C2 myocytes following hypoxia.

    abstract::Hypoxia may induce apoptosis and autophagy to promote cardiomyocyte injury. The present study investigated the effect of berberine, a natural extract of Rhizoma Coptidis, on hypoxia‑induced autophagy and apoptosis in the H9c2 rat myocardial cell line. Expression levels of apoptosis and autophagy markers were upregulat...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Jia Z,Lin L,Huang S,Zhu Z,Huang W,Huang Z

    更新日期:2017-08-01 00:00:00

  • Cathepsin S regulates renal fibrosis in mouse models of mild and severe hydronephrosis.

    abstract::As a member of the cysteine protease family, cathepsin S (CTSS) serves an important role in diseases such as cancer, arthritis and atherosclerosis. Nevertheless, its role in renal fibrosis is unknown. In the present study, the effects of CTSS on renal fibrosis in mild (group M) and severe (group S) hydronephrosis were...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Yao X,Cheng F,Yu W,Rao T,Li W,Zhao S,Zhou X,Ning J

    更新日期:2019-07-01 00:00:00

  • Differential effects of calcium antagonists on ABCG2/BCRP-mediated drug resistance and transport in SN-38-resistant HeLa cells.

    abstract::The effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport were examined in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP. Sensitivity to mitoxantrone, an ABCG2/BCRP substrate, in HeLa/SN100 cells was significantly reversed by ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Takara K,Matsubara M,Yamamoto K,Minegaki T,Takegami S,Takahashi M,Yokoyama T,Okumura K

    更新日期:2012-03-01 00:00:00

  • Formononetin attenuates monocrotaline‑induced pulmonary arterial hypertension via inhibiting pulmonary vascular remodeling in rats.

    abstract::Pulmonary arterial hypertension (PAH) is a life‑threatening disease induced by the excessive proliferation and reduced apoptosis of pulmonary artery smooth muscle cells (PASMCs). Formononetin (FMN) is a natural isoflavone with numerous cardioprotective properties, which can inhibit the proliferation and induce the apo...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Cai C,Xiang Y,Wu Y,Zhu N,Zhao H,Xu J,Lin W,Zeng C

    更新日期:2019-12-01 00:00:00

  • Gastric bypass surgery alters the mechanisms of insulin resistance in the adipose tissue of GK rats.

    abstract::Roux-en-Y gastric bypass (RYGB) has become an effective treatment for type 2 diabetes mellitus (T2DM). However, the blood glucose mechanism involved remains unclear. In this study, 60 male Goto-Kakizaki (GK) rats were randomly divided into 6 groups (each with 10 rats): the GB1/2 gro...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Li J,Wang Y,Zhou Y,Liu J

    更新日期:2012-11-01 00:00:00

  • Effect of OLIG1 on the development of oligodendrocytes and myelination in a neonatal rat PVL model induced by hypoxia-ischemia.

    abstract::OLIG1 is an oligodendrocyte (OL) transcription factor, which can contribute to the proliferation and differentiation of OLs, and the maturation of myelin. The aim of this study was to clarify the role of OLIG1 in neonatal Sprague Dawley rats with periventricular leukomalacia (PVL), induced by hypoxia‑ischemia (HI). Ne...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Cheng T,Xue X,Fu J

    更新日期:2015-04-01 00:00:00

  • Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis.

    abstract::Interleukin (IL)-1β-induced chondrocyte apoptosis is associated with the pathogenesis of arthritis. Platelet‑rich plasma (PRP), which is derived from the patient's own blood and contains numerous growth factors, has the potential for arthritis treatment. Therefore, the present study aimed to determine the effects of P...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Yang J,Lu Y,Guo A

    更新日期:2016-11-01 00:00:00

  • Epigallocatechin-3-gallate and zinc provide anti-apoptotic protection against hypoxia/reoxygenation injury in H9c2 rat cardiac myoblast cells.

    abstract::It has previously been demonstrated that phosphatidylinositol-3-kinase (PI3K)/Akt and cleaved caspase-3 serve critical roles in the apoptosis of cardiac myocytes following ischemia/reperfusion injury. Epigallocatechin-3-gallate (EGCG), the predominant catechin component of green tea, has been reported to have potentia...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Zeng X,Tan X

    更新日期:2015-08-01 00:00:00

  • FGF signaling contributes to atherosclerosis by enhancing the inflammatory response in vascular smooth muscle cells.

    abstract::The contractile to synthetic phenotypic switching of vascular smooth muscle cells (VSMCs) in response to fibroblast growth factor (FGF) has been previously described. However, the role of the inflammatory response induced by FGF signaling in VSMCs and its occurrence in atherosclerosis remains unclear. In the present s...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Qi M,Xin S

    更新日期:2019-07-01 00:00:00

  • Galectin‑3 induces the phenotype transformation of human vascular smooth muscle cells via the canonical Wnt signaling.

    abstract::Galectin‑3, a galactoside‑binding protein, is highly expressed in carotid plaques and plays an important role in the atherosclerotic lesions. The phenotype transformation of vascular smooth muscle cells is the basic pathological change of atherosclerosis. This study investigated the effects of exogenous galectin‑3 on ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Tian L,Chen K,Cao J,Han Z,Wang Y,Gao L,Fan Y,Wang C

    更新日期:2017-06-01 00:00:00

  • Differentially expressed genes on the growth of mouse Leydig cells treated with standardised Eurycoma longifolia extract.

    abstract::Eurycoma (E.) longifolia Jack (Tongkat Ali) is a widely applied medicine that has been reported to boost serum testosterone and increase muscle mass. However, its actual biological targets and effects on an in vitro level remain poorly understood. Therefore, the present study aimed to investigate the effects of a stan...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Khurshid Ahmed NA,Lim SK,Pandian GN,Sugiyama H,Lee CY,Khoo BY,Chan KL

    更新日期:2020-11-01 00:00:00

  • TRESK gene recombinant adenovirus vector inhibits capsaicin-mediated substance P release from cultured rat dorsal root ganglion neurons.

    abstract::The present study was conducted to determine whether the activation of TRESK in the dorsal root ganglion (DRG) by the TRESK gene recombinant adenovirus vector inhibits the capsaicin-evoked substance P (SP) release using a radioimmunoassay. TRESK is an outwardly rectifying K+ current...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Zhou J,Yao SL,Yang CX,Zhong JY,Wang HB,Zhang Y

    更新日期:2012-04-01 00:00:00

  • Expression and function of microRNA-497 in human osteosarcoma.

    abstract::The expression and function of microRNA-497 (miR-497) has previously been reported in various types of human cancer; however, miR-497 has not previously been investigated in human osteosarcoma (OS). In the present study, the expression levels of miR‑497 were analyzed by reverse transcription‑quantitative polymerase ch...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Liu Q,Wang H,Singh A,Shou F

    更新日期:2016-07-01 00:00:00

  • MicroRNA-106a confers cisplatin resistance in non-small cell lung cancer A549 cells by targeting adenosine triphosphatase-binding cassette A1.

    abstract::MicroRNAs (miRNAs) have been discovered to have pivotal roles in regulating the drug resistance of various types of human cancer, including cisplatin (DDP) resistance in non-small cell lung cancer (NSCLC). Fewer studies have explored the roles of miR-106a in NSCLC-cell resistance to DDP and its precise molecular mecha...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Ma Y,Li X,Cheng S,Wei W,Li Y

    更新日期:2015-01-01 00:00:00

  • Comparative proteomic analysis of ductal breast carcinoma demonstrates an altered expression of chaperonins and cytoskeletal proteins.

    abstract::The aim of the present study was to analyze the protein composition of ductal breast carcinoma and the surrounding normal tissue in individual patients using comparative 2D proteomics and mass spectrometry to detect candidate disease biomarkers for diagnosis and prognosis. Samples of normal and cancerous tissue obtain...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Carcoforo P,Ura B,Mischiati C,Squerzanti M,Lanzara V,Cervellati C,Calza R,De Laureto PP,Frare E,Portinari M,Feriotto G,Lanzara S,Agostinelli E,Bergamini CM

    更新日期:2013-05-01 00:00:00

  • Mechanism and therapeutic effects of Saccharomyces boulardii on experimental colitis in mice.

    abstract::Inflammatory bowel disease (IBD) is a type of chronic inflammatory disturbance that affects a number of individuals worldwide; the precise mechanism is unclear and treatment is frequently insufficient to maintain patients in remission. Saccharomyces boulardii is a thermophilic, non‑pathogenic yeast that may be adminis...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Zhou H,Zhang HJ,Guan L,Zhang YN,Li Y,Sun MJ

    更新日期:2018-12-01 00:00:00

  • TAK‑242 exerts a neuroprotective effect via suppression of the TLR4/MyD88/TRIF/NF‑κB signaling pathway in a neonatal hypoxic‑ischemic encephalopathy rat model.

    abstract::Neonatal hypoxic-ischemic encephalopathy (HIE) is one of the main causes of death and nervous system damage in neonates. The aim of the present study was to investigate the effect of the Toll‑like receptor 4 (TLR4) antagonist TAK‑242 on HIE. The Rice‑Vannucci method was used for ligation of the left common carotid art...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Jiang L,Xu Z,Li H,Wu M,Wang F,Liu S,Tao J,Feng X

    更新日期:2020-08-01 00:00:00

  • miRNAs and their role in the correlation between schizophrenia and cancer (Review).

    abstract::Schizophrenia (SZ) and cancer (Ca) have a broad spectrum of clinical phenotypes and a complex biological background, implicating a large number of genetic and epigenetic factors. SZ is a chronic neurodevelopmental disorder signified by an increase in the expression of apoptotic molecular signals, whereas Ca is convers...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章,评审


    authors: Rizos E,Siafakas N,Skourti E,Papageorgiou C,Tsoporis J,Parker TH,Christodoulou DI,Spandidos DA,Katsantoni E,Zoumpourlis V

    更新日期:2016-12-01 00:00:00

  • Inhibitory activity of Socheongryong‑tang and its constituent components against the production of RANTES, eotaxin, eotaxin‑3 and MMP‑9 from BEAS‑2B cells.

    abstract::Socheongryeong‑tang (SCRT) is a herbal formula previously used to treat pulmonary diseases primarily caused by the common cold virus, including airway inflammation, asthma and allergy. The aim of the present study was to investigate the inhibitory effect of SCRT water extract and its 13 constituent components on chemo...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Kim JH,Jeon WY,Lee MY,Seo CS,Lim HS,Shin HK

    更新日期:2014-12-01 00:00:00

  • Hydrogen-rich saline improves non‑alcoholic fatty liver disease by alleviating oxidative stress and activating hepatic PPARα and PPARγ.

    abstract::Non-alcoholic fatty liver disease (NAFLD) comprises a range of liver diseases, between steatosis and non‑alcoholic steatohepatitis and liver cirrhosis, which are closely associated with diabetes mellitus. Previous studies have indicated that oxidative stress is a key factor in the development of NAFLD. Molecular hydro...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Zhai X,Chen X,Lu J,Zhang Y,Sun X,Huang Q,Wang Q

    更新日期:2017-03-01 00:00:00

  • Polyaniline promotes peripheral nerve regeneration by enhancement of the brain‑derived neurotrophic factor and ciliary neurotrophic factor expression and activation of the ERK1/2/MAPK signaling pathway.

    abstract::A previous study has demonstrated a progression in the nerve regeneration by polyaniline/cellulose (PANI/RC), although the underlying mechanism was not elucidated. In the present study, regenerated nerves were investigated, using histological techniques, functional assays and western blot analysis. The triceps surae m...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Fan L,Xiong Y,Fu Z,Xu D,Wang L,Chen Y,Xia H,Peng N,Ye S,Wang Y,Zhang L,Ye Q

    更新日期:2017-11-01 00:00:00

  • Mutation of 3β-hydroxysteroid dehydrogenase (3β-HSD) at the 3'-untranslated region is associated with adrenocortical insufficiency.

    abstract::In the present study, we report the mutation of the 3β-hydroxysteroid dehydrogenase (3β-HSD) gene in a family suffering from adrenocortical insufficiency. The index patient was clinically diagnosed with adrenocortical insufficiency. Peripheral venous blood (5 ml) was collected from ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Pan Y,Zhong S,Hu RM,Gong W

    更新日期:2012-12-01 00:00:00

  • Effects of isomaltulose on insulin resistance and metabolites in patients with non‑alcoholic fatty liver disease: A metabolomic analysis.

    abstract::Insulin resistance is associated with a poor prognosis in non‑alcoholic fatty liver disease (NAFLD) patients. Isomaltulose, a naturally‑occurring disaccharide, is reported to improve glucose and lipid metabolisms in obese patients. The present study aimed to investigate the effects of isomaltulose on insulin resistanc...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章,随机对照试验


    authors: Kawaguchi T,Nakano D,Oriishi T,Torimura T

    更新日期:2018-08-01 00:00:00