Phosphoinositide 3-kinase/Akt pathway is involved in pingyangmycin‑induced growth inhibition, apoptosis and reduction of invasive potential in EOMA mouse hemangioendothelioma cells.


:Pingyangmycin (PYM), a glycopeptide antibiotic, has been recommended as a stand treatment for hemangioma. However, the underlying mechanisms of its anti‑tumor effects have remained elusive. The purpose of the present study was to explore the effects of PYM on the biological behavior of the EOMA mouse hemangioendothelioma cell line and investigate the possible mechanisms. The effects of PYM on EOMA cell viability were determined by an MTT assay, apoptosis was evaluated by Annexin V/propidium iodide staining and flow cytometric analysis, and cell invasion ability was determined using a Transwell invasion assay. In order to investigate the underlying mechanism of action of PYM, the expression of angiogenic signaling proteins was determined by western blot analysis. PYM treatment (0.5‑500 µg/ml) inhibited cell growth in a time- and dose‑dependent manner. PYM at 100 µg/ml significantly induced apoptosis and reduced the invasive ability of EOMA cells. Effects of PYM on cell viability, apoptosis and invasion ability were completely blocked by co‑treatment with phosphoinositide 3‑kinase (PI3K) activator insulin‑like growth factor‑1 (IGF‑1). Furthermore, treatment with PYM reduced the expression of PI3K and phosphorylated Akt. In conclusion, the present study indicated that the PI3K/Akt pathway is likely to be involved in the anti-cancer effects of PYM on EOMA cells.


Mol Med Rep


Peng LX,Zhao P,Zhao HS,Pan E,Yang BB,Li Q




Has Abstract


2015-12-01 00:00:00












  • Identification of key genes and pathways in regulating immune‑induced diseases of dendritic cells by bioinformatic analysis.

    abstract::Dendritic cells (DCs) serve crucial roles in the activation of the immune response, and imbalance in the activation or inhibition of DCs has been associated with an increased susceptibility to develop immune‑induced diseases. However, the molecular mechanisms of regulating immune‑induced diseases of DCs are not well u...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Zheng Y,Zheng X,Li S,Zhang H,Liu M,Yang Q,Zhang M,Sun Y,Wu J,Yu B

    更新日期:2018-06-01 00:00:00

  • Protective effects of camel milk against pathogenicity induced by Escherichia coli and Staphylococcus aureus in Wistar rats.

    abstract::The aim of the present study was to investigate the protective effects of camel milk on hepatic pathogenicity induced by experimental infection with Escherichia (E. coli) and Staphylococcus aureus (S. aureus) in Wistar rats. The rats were divided into six groups: The control and camel milk groups received water and ca...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Soliman MM,Hassan MY,Mostafa SA,Ali HA,Saleh OM

    更新日期:2015-12-01 00:00:00

  • Overexpression of NAC1 confers drug resistance via HOXA9 in colorectal carcinoma cells.

    abstract::Colorectal carcinoma (CRC) is one of the most common types of malignancy worldwide. Recently, neoadjuvant chemotherapy has become an important treatment strategy for CRC. However, treatment frequently fails due to the development of chemoresistance, which is a major obstacle for positive prognosis. However, the underl...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Ju T,Jin H,Ying R,Xie Q,Zhou C,Gao D

    更新日期:2017-09-01 00:00:00

  • MicroRNA‑142‑5p modulates breast cancer cell proliferation and apoptosis by targeting phosphatase and tensin homolog.

    abstract::A total of 60 breast cancer (BC) tissues and adjacent healthy tissues from patients who underwent surgery in Renmin Hospital of Wuhan University were collected for analysis in the present study. Results from reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) demonstrated that, compared with the adj...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Xu W,Wang W

    更新日期:2018-06-01 00:00:00

  • Association of BAFF with PI3K/Akt/mTOR signaling in lupus nephritis.

    abstract::Systemic lupus erythematosus is a connective tissue disease characterized by autoimmune inflammation, which leads to specific and nonspecific immune disorders with the formation of various autoantibodies by activated B cells. B‑cell‑activating factor (BAFF) is secreted by macrophages and activated T cells, and is resp...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Ge F,Wang F,Yan X,Li Z,Wang X

    更新日期:2017-11-01 00:00:00

  • Synergistic neuroprotective effect of microglial‑conditioned media treated with geniposide and ginsenoside Rg1 on hypoxia injured neurons.

    abstract::The synergistic mechanism underlying the effects of multi‑component combined drug use for complex diseases remains to be fully elucidated. Microglial activation following ischemia can either affect neural survival or cause neuronal injury. The aim of the present study was to determine the synergistic effect of genipos...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Wang J,Hou J,Lei H,Fu J,Pan Y,Liu J

    更新日期:2015-10-01 00:00:00

  • Distinct peak at 3.8 ppm observed by 3T MR spectroscopy in meningiomas, while nearly absent in high-grade gliomas and cerebral metastases.

    abstract::The purpose of the present study was to evaluate distinct metabolic features of meningiomas to distinguish them from other brain lesions using proton magnetic resonance spectroscopy. The study was performed on 17 meningiomas, 24 high-grade gliomas and 9 metastases. Elevated signal intensity at 3.8 ppm observed in low ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Kousi E,Tsougos I,Fountas K,Theodorou K,Tsolaki E,Fezoulidis I,Kapsalaki E

    更新日期:2012-04-01 00:00:00

  • Protective effects of atorvastatin on cerebral vessel autoregulation in an experimental rabbit model of subarachnoid hemorrhage.

    abstract::The aim of the present study was to assess the therapeutic effects of atorvastatin on cerebral vessel autoregulation and to explore the underlying mechanisms in a rabbit model of subarachnoid hemorrhage (SAH). A total of 48 healthy male New Zealand rabbits (weight, 2‑2.5 kg) were randomly allocated into SAH, Sham or S...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Chen JH,Wu T,Yang LK,Chen L,Zhu J,Li PP,Hu X,Wang YH

    更新日期:2018-01-01 00:00:00

  • Aberrant miRNA expression response to UV irradiation in human liver cancer cells.

    abstract::microRNAs (miRNAs) are small, highly conserved, non-coding RNAs that regulate gene expression at the post‑transcriptional level. The expression of these small RNA genes is tightly regulated during development, differentiation and apoptosis of normal cells, however, they are often deregulated in various types of cancer...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Liang G,Li G,Wang Y,Lei W,Xiao Z

    更新日期:2014-03-01 00:00:00

  • Effects of human vascular endothelial growth factor on reparative dentin formation.

    abstract::It is a challenge for dentists to save dental pulp in patients with pulp disease without resorting to root canal therapy. Formation of tertiary dentin to maintain pulp vitality is a key odontoblast response to dental pulp injury. Vascular endothelial growth factor (VEGF) is the most potent angiogenic and vasculogenic ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Zhang J,Liu X,Yu W,Zhang Y,Shi C,Ni S,Liu Q,Li X,Sun Y,Zheng C,Sun H

    更新日期:2016-01-01 00:00:00

  • Effects of various doses of atorvastatin on vascular endothelial cell apoptosis and autophagy in vitro.

    abstract::Atorvastatin (Lipitor™) is a lipid‑lowering agent that is widely used in the treatment of cardiovascular diseases. Previous research has largely focused on its cholesterol‑lowering effects; however, a limited number of studies have investigated the actions of atorvastatin on vascular endothelial cells. In the present ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Zhao WB,Fu H,Chang F,Liu J,Wang J,Li F,Zhao J

    更新日期:2019-03-01 00:00:00

  • Knockdown of Y‑box‑binding protein‑1 inhibits the malignant progression of HT‑29 colorectal adenocarcinoma cells by reversing epithelial‑mesenchymal transition.

    abstract::Y‑box binding protein‑1 (YB‑1) has been identified as an oncoprotein in various malignancies. The aim of this study was to investigate the biological role of YB‑1 and its association with epithelial‑to‑mesenchymal transition (EMT) in colorectal cancer (CRC). The expression of YB‑1 and three EMT‑related proteins (E‑cad...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Yan XB,Zhu QC,Chen HQ,Peng JY,Chao HL,Du HX,Wang ZG,Jin ZM

    更新日期:2014-11-01 00:00:00

  • Combination of branched-chain amino acid and angiotensin-converting enzyme inhibitor improves liver fibrosis progression in patients with cirrhosis.

    abstract::An effective therapeutic strategy for suppressing liver fibrosis should improve the overall prognosis of patients with chronic liver diseases. Although enormous efforts are ongoing to develop anti-fibrotic agents, no drugs have yet been approved as anti-fibrotic agents for humans. Insulin resistance (IR) is reportedly...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章,随机对照试验


    authors: Yoshiji H,Noguchi R,Ikenaka Y,Kaji K,Aihara Y,Douhara A,Yamao J,Toyohara M,Mitoro A,Sawai M,Yoshida M,Morioka C,Fujimoto M,Uemura M,Fukui H

    更新日期:2012-02-01 00:00:00

  • Angiotensin II promotes differentiation of mouse c-kit-positive cardiac stem cells into pacemaker-like cells.

    abstract::Cardiac stem cells (CSCs) can differentiate into cardiac muscle‑like cells; however, it remains unknown whether CSCs may possess the ability to differentiate into pacemaker cells. The aim of the present study was to determine whether angiotensin II (Ang II) could promote the specialization of CSCs into pacemaker‑like ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Xue C,Zhang J,Lv Z,Liu H,Huang C,Yang J,Wang T

    更新日期:2015-05-01 00:00:00

  • Trichostatin A‑induced miR‑30a‑5p regulates apoptosis and proliferation of keloid fibroblasts via targeting BCL2.

    abstract::Keloids are benign fibrous overgrowths that occur as a result of abnormal wound healing following cutaneous injury. MicroRNAs (miRNAs/miRs) are short non‑coding RNAs that serve critical roles in numerous important biological processes, such as cell proliferation, differentiation and apoptosis. However, their role in k...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Jian X,Qu L,Wang Y,Zou Q,Zhao Q,Chen S,Gao X,Chen H,He C

    更新日期:2019-06-01 00:00:00

  • Transforming growth factor-β1 induces fibrosis in rat meningeal mesothelial cells via the p38 signaling pathway.

    abstract::Leptomeningeal fibrosis is important in the pathogenesis of communicating hydrocephalus following subarachnoid hemorrhage; however, the underlying mechanisms of leptomeningeal fibrosis remain largely unclear. In the present study, primary meningeal mesothelial cells (MMCs) were used as a cell model to investigate the ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Yue XJ,Guo Y,Yang HJ,Feng ZW,Li T,Xu YM

    更新日期:2016-08-01 00:00:00

  • Rhus javanica Linn protects against hydrogen peroxide‑induced toxicity in human Chang liver cells via attenuation of oxidative stress and apoptosis signaling.

    abstract::Rhus javanica Linn, a traditional medicinal herb from the family Anacardiaceae, has been used in the treatment of liver diseases, cancer, parasitic infections, malaria and respiratory diseases in China, Korea and other Asian countries for centuries. In the present study, the protective effects of R. javanica ethanolic...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Yoon C,Koppula S,Yoo S,Yum M,Kim J,Lee J,Song M

    更新日期:2016-01-01 00:00:00

  • Artesunate suppresses oxidative and inflammatory processes by activating Nrf2 and ROS‑dependent p38 MAPK and protects against cerebral ischemia‑reperfusion injury.

    abstract::Artesunate is a semi-synthetic derivative of artemisinin that is used in the treatment of patients with malaria. Artesunate has also been reported to exert immune‑regulatory, antitumor, hepatoprotective, anti‑inflammatory and smooth muscle relaxing functions. The present study aimed to investigate the putative protect...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Lu H,Wang B,Cui N,Zhang Y

    更新日期:2018-05-01 00:00:00

  • Combination of oncolytic adenovirus and luteolin exerts synergistic antitumor effects in colorectal cancer cells and a mouse model.

    abstract::In recent years, oncolytic viruses have attracted increasing interest due to their potent antitumor effects. Luteolin, a natural product, has additionally been observed to exhibit various pharmacological antitumor activities. Previously, a novel dual‑targeting oncolytic adenovirus, complement decay‑accelerating factor...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Xiao B,Qin Y,Ying C,Ma B,Wang B,Long F,Wang R,Fang L,Wang Y

    更新日期:2017-12-01 00:00:00

  • Effects of cyclin E gene silencing on the proliferation of esophageal cancer cell lines, EC9706, Eca109 and KYSE30.

    abstract::In order to observe the effects of cyclin E gene silencing by small interfering RNA (siRNA) on the growth, proliferation, invasion and apoptosis of esophageal cancer cell lines, including EC9706, Eca109 and KYSE30, siRNA vectors targeting cyclin E gene were constructed and then tran...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Wang N,Li M,Zang WQ,Ma YY,Wang YY,Zhao GQ

    更新日期:2013-03-01 00:00:00

  • Whole exome sequencing identified a pathogenic nonsense mutation in LMNA in a family with a progressive cardiac conduction defect: A case report.

    abstract::Progressive cardiac conduction defect (PCCD) is an inherited autosomal dominant cardiac disorder characterized by an age‑dependent cardiac electrical conduction block. Several genes have been associated with the genetic pathogenesis of PCCD. The present study aimed to identify the causal mutation of PCCD and to invest...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Fan P,Zhang D,Yang KQ,Tian T,Luo F,Liu YX,Wang LP,Zhou XL

    更新日期:2020-06-01 00:00:00

  • Chemotherapy oxaliplatin sensitizes prostate cancer to immune checkpoint blockade therapies via stimulating tumor immunogenicity.

    abstract::Even though standard treatment options are available for prostate cancer patients, prostate cancer is still a leading cause of death in many Western countries due to drug resistance and recurrence. Immune checkpoint blockade therapy has been proved to be very effective in some melanoma patients, which might dependent ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Zhou J,Yang T,Liu L,Lu B

    更新日期:2017-09-01 00:00:00

  • Prediction and identification of B‑cell epitopes for tumor necrosis factor‑α.

    abstract::The aim of the present study was to predict and identify B‑cell epitopes for mouse tumor necrosis factor‑α (mTNF‑α). DNAStar and BcePred software were used to predict B‑cell epitopes for mTNF‑α. A predicted eight‑branch multiple antigenic polypeptide (MAP) was synthesized and used to immunize BALB/c mice, combined wit...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Zhang J,Cui Y,Wu Y,Wang H,Ke J

    更新日期:2017-09-01 00:00:00

  • Proteins S100A8 and S100A9 are potential biomarkers for renal cell carcinoma in the early stages: results from a proteomic study integrated with bioinformatics analysis.

    abstract::In order to investigate the two members of the EF‑hand Ca2+ binding protein S100 family, S100A8 and S100A9, in renal cell carcinoma (RCC), serum samples were collected from patients with RCC, transitional cell carcinoma in the kidney, benign renal masses and normal controls. The samples were analyzed by isobaric tags ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Zhang L,Jiang H,Xu G,Wen H,Gu B,Liu J,Mao S,Na R,Jing Y,Ding Q,Zhang Y

    更新日期:2015-06-01 00:00:00

  • Identification of microRNAome in rat bladder reveals miR-1949 as a potential inducer of bladder cancer following spinal cord injury.

    abstract::The costs of spinal cord injury and its complications are high in personal, social and financial terms. Complications include bladder cancer, for which the risk is 16-28 times higher than that of the general population, There is currently little consensus regarding the cause of this discrepancy. As microRNAs are stabl...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Wang T,Liu Y,Yuan W,Zhang L,Zhang Y,Wang Z,Zhou X,Zhou H,Chu T,Hao Y,Liu B,Zhao X,Lu L,Feng S,Kong X

    更新日期:2015-08-01 00:00:00

  • TGF‑β signaling: A complex role in tumorigenesis (Review).

    abstract::Tumor progression can be affected by various cellular components of tumor cells and/or by tumor microenvironmental factors. The tumor microenvironment comprises a variety of nonmalignant stromal cells and inflammatory cytokines, which are pivotal in tumor promotion and progression. The transforming growth factor‑β (TG...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章,评审


    authors: Liu S,Chen S,Zeng J

    更新日期:2018-01-01 00:00:00

  • Fas expression is downregulated in gastric cancer.

    abstract::The aim of the present study was to investigate Fas expression in tumor samples from patients with gastric cancer, in order to determine the involvement of the Fas signaling pathway. The protein expression levels of Fas, caspase-8, caspase‑3 and poly (adenosine diphosphate‑ribose) polymerase 1 (PARP1) were examined in...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Wang X,Fu Z,Chen Y,Liu L

    更新日期:2017-02-01 00:00:00

  • INSIG2 rs7566605 single nucleotide variant and global DNA methylation index levels are associated with weight loss in a personalized weight reduction program.

    abstract::Single nucleotide polymorphisms associated with lipid metabolism and energy balance are implicated in the weight loss response caused by nutritional interventions. Diet‑induced weight loss is also associated with differential global DNA methylation. DNA methylation has been proposed as a predictive biomarker for weigh...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Pirini F,Rodriguez-Torres S,Ayandibu BG,Orera-Clemente M,Gonzalez-de la Vega A,Lawson F,Thorpe RJ Jr,Sidransky D,Guerrero-Preston R

    更新日期:2018-01-01 00:00:00

  • Intervertebral disc regeneration using platelet‑rich plasma‑containing bone marrow‑derived mesenchymal stem cells: A preliminary investigation.

    abstract::Platelet‑rich plasma (PRP) is a promising strategy for intervertebral disc degeneration (IDD). However, the short half‑life of growth factors released from PRP cannot continuously stimulate the degenerated discs. Thus, the present study hypothesized that the combined use of PRP and bone marrow‑derived mesenchymal stem...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Wang SZ,Jin JY,Guo YD,Ma LY,Chang Q,Peng XG,Guo FF,Zhang HX,Hu XF,Wang C

    更新日期:2016-04-01 00:00:00

  • 5' and 3' UTR thymidylate synthase polymorphisms modulate the risk of colorectal cancer independently of the intake of methyl group donors.

    abstract::Thymidylate synthase, as a rate-limiting step in DNA synthesis, catalyses the conversion of dUMP into dTMP using 5,10-methylenotetrahydrofolate as the methyl donor. Two polymorphisms have been described in this gene: a repeat polymorphism in the 5' promoter enhancer region (3R versus 2R) and a 6 bp deletion in the 3' ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章


    authors: Carmona B,Guerreiro C,Cravo M,Nobre-Leitão C,Brito M

    更新日期:2008-09-01 00:00:00