Chloride intracellular channel 1 regulates migration and invasion in gastric cancer by triggering the ROS-mediated p38 MAPK signaling pathway.

Abstract:

:Chloride intracellular channel 1 (CLIC1) has been demonstrated to be overexpressed in gastric cancer, and elevated CLIC1 expression levels are markedly associated with the processes of tumor cell migration and invasion. However, the regulatory mechanism and signaling pathway underlying these processes have remained to be elucidated. The present study examined the impact of N-acetyl cysteine (NAC), indanyloxyacetic acid (IAA)-94 and SB203580, inhibitors of reactive oxygen species (ROS), as well as CLIC1 and p38 mitogen-activated protein kinase (MAPK) on the migration and invasion of SGC-7901 gastric cancer cells in a hypoxia‑reoxygenation (H-R) microenvironment. The results demonstrated that intracellular ROS and CLIC1 levels were increased under H‑R conditions, and that functional inhibition of CLIC1 significantly decreased the H‑R‑elevated ROS generation and p‑p38 MAPK levels in SGC‑7901 cells, as well as inhibited the migration and invasion of SGC‑7901 cells. In addition, the expression levels of MMP‑2 and MMP‑9 were inhibited by NAC, IAA‑94 and SB203580. These results indicated that CLIC1 regulates gastric cancer-cell migration and invasion via the ROS-mediated p38 MAPK signaling pathway.

journal_name

Mol Med Rep

authors

Zhao W,Lu M,Zhang Q

doi

10.3892/mmr.2015.4459

subject

Has Abstract

pub_date

2015-12-01 00:00:00

pages

8041-7

issue

6

eissn

1791-2997

issn

1791-3004

journal_volume

12

pub_type

杂志文章
  • Survivin is expressed in early hepatocellular carcinoma and surrounding hepatitis tissue.

    abstract::It has been suggested that survivin, a member of the inhibitor of apoptosis protein (IAP) family, is an attractive novel target for anticancer therapy. The present study investigated the expression of survivin during the early stages of hepatocellular carcinoma (HCC) and associated hepatitis, and attempted to elucidat...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr_00000191

    authors: Mamori S,Matsushima M,Matsuura T,Tajiri H

    更新日期:2009-11-01 00:00:00

  • Influence of microRNA‑34a on proliferation, invasion and metastasis of HCT116 cells.

    abstract::The aim of the present study was to investigate the role of microRNA (miR)‑34a expression in the proliferation, invasion and metastasis of colon cancer and its underlying mechanisms. HCT116 cells were cultured in high‑sugar Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum and 1000 U/ml penic...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.6088

    authors: Li C,Lu S,Wang Y,Guo S,Zhao T,Wang X,Song B

    更新日期:2017-02-01 00:00:00

  • Effects of butylphthalide on cognitive decline in diabetic rats.

    abstract::Butylphthalide, a component extracted from seeds of Chinese celery, is an effective neuroprotective agent used for the treatment of ischemic stroke and dementia. Diabetes may cause central nervous system damage, and diabetes is closely associated with dementia. The aim of the present study was to investigate the effec...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7700

    authors: Tian Z,Wang J,Wang Y,Zhang M,Zhou Y

    更新日期:2017-12-01 00:00:00

  • Tetraspanin family identified as the central genes detected in gastric cancer using bioinformatics analysis.

    abstract::Gastric cancer has become a serious disease in the past decade. It has the second highest mortality rate among the four most common cancer types, leading to ~700,000 mortalities annually. Previous studies have attempted to elucidate the underlying biological mechanisms of gastric cancer. The present study aimed to obt...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9360

    authors: Qi W,Sun L,Liu N,Zhao S,Lv J,Qiu W

    更新日期:2018-10-01 00:00:00

  • TBX5 loss-of-function mutation contributes to atrial fibrillation and atypical Holt-Oram syndrome.

    abstract::Previous genome-wide association studies have demonstrated that single nucleotide polymorphisms in T‑box (TBX)5 are associated with increased susceptibility to atrial fibrillation (AF), and a recent study has causally linked a TBX5 mutation to atypical Holt-Oram syndrome and paroxysmal AF. However, the prevalence and ...

    journal_title:Molecular medicine reports

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.3892/mmr.2016.5043

    authors: Guo DF,Li RG,Yuan F,Shi HY,Hou XM,Qu XK,Xu YJ,Zhang M,Liu X,Jiang JQ,Yang YQ,Qiu XB

    更新日期:2016-05-01 00:00:00

  • Silencing cyclin-dependent kinase inhibitor 3 inhibits the migration of breast cancer cell lines.

    abstract::Cyclin-dependent kinase inhibitor 3 (CDKN3) belongs to the dual-specificity protein phosphatase family, which is hypothesized to regulate cell cycle progression in tumor cells. However, whether CDKN3 is a potential therapeutic target for breast cancer remains to be elucidated. The present in vitro study aimed to inves...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5401

    authors: Deng M,Wang J,Chen Y,Zhang L,Xie G,Liu Q,Zhang T,Yuan P,Liu D

    更新日期:2016-08-01 00:00:00

  • Circulating miRNA‑21 is a promising biomarker for heart failure.

    abstract::microRNA 21 (miRNA‑21) promotes the development of cardiac fibrosis, hypertrophy and heart failure. However, whether it can be used as a biomarker for the diagnosis and prognosis of heart failure remains unclear. The current study assessed circulating miRNA‑21 as a viable indicator for diagnosis and prognosis of heart...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7575

    authors: Zhang J,Xing Q,Zhou X,Li J,Li Y,Zhang L,Zhou Q,Tang B

    更新日期:2017-11-01 00:00:00

  • Photoprotective effect of arctiin against ultraviolet B-induced damage in HaCaT keratinocytes is mediated by microRNA expression changes.

    abstract::Human keratinocytes are located in the outermost skin layer and thus particularly vulnerable to ultraviolet B (UVB) radiation exposure. Previous studies have focused on the cellular and molecular perspectives of UVB-induced keratinocyte damage. In the present study, it was demonstrated that pretreatment with the phyto...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2326

    authors: Cha HJ,Lee GT,Lee KS,Lee KK,Hong JT,Lee NK,Kim SY,Lee BM,An IS,Hahn HJ,Ahn KJ,Lee SJ,An S,Bae S

    更新日期:2014-09-01 00:00:00

  • Effects of various doses of atorvastatin on vascular endothelial cell apoptosis and autophagy in vitro.

    abstract::Atorvastatin (Lipitor™) is a lipid‑lowering agent that is widely used in the treatment of cardiovascular diseases. Previous research has largely focused on its cholesterol‑lowering effects; however, a limited number of studies have investigated the actions of atorvastatin on vascular endothelial cells. In the present ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.9828

    authors: Zhao WB,Fu H,Chang F,Liu J,Wang J,Li F,Zhao J

    更新日期:2019-03-01 00:00:00

  • Microarray data analysis of neuroblastoma: Expression of SOX2 downregulates the expression of MYCN.

    abstract::The present study aimed to identify the genes directly or indirectly correlated with the amplification of MYCN in neuroblastoma (NB). Microarray data (GSE53371) were downloaded from Gene Expression Omnibus, and included 10 NB cell lines with MYCN amplification and 10 NB cell lines with normal MYCN copy numbers. Differ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4311

    authors: Bao J,Qin L,Cui L,Wang X,Meng Q,Zhu L,Zhang S

    更新日期:2015-11-01 00:00:00

  • Construction of SET overexpression vector and its effects on the proliferation and apoptosis of 293T cells.

    abstract::The expression of SET nuclear proto‑oncogene (SET) is commonly associated with cell proliferation and tumorigenesis. In the present study, a eukaryotic SET expression plasmid (pEGFP‑N1‑SET) was constructed and transiently transfected into 293T human embryonic kidney cells. Transfection led to expression of the SET onc...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5049

    authors: Wang Y,He PC,Liu YF,Qi J,Zhang M

    更新日期:2016-05-01 00:00:00

  • Knockdown of biglycan expression by RNA interference inhibits the proliferation and invasion of, and induces apoptosis in, the HCT116 colon cancer cell line.

    abstract::Biglycan is an important component of the extracellular matrix, and it is also a member of small leucine-rich proteoglycan family. Previous studies indicated that the expression of biglycan was increased in a variety of tumor tissues, including colon cancer. However, the mechanisms underlying its effects in colon canc...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4383

    authors: Xing X,Gu X,Ma T

    更新日期:2015-11-01 00:00:00

  • Bioinformatics analysis of mRNA and miRNA microarray to identify the key miRNA‑gene pairs in small‑cell lung cancer.

    abstract::Small‑cell lung cancer (SCLC) is a type of lung cancer with early metastasis, and high recurrence and mortality rates. The molecular mechanism is still unclear and further research is required. The aim of the present study was to examine the pathogenesis and potential molecular markers of SCLC by comparing the differe...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10441

    authors: Mao Y,Xue P,Li L,Xu P,Cai Y,Chu X,Jiang P,Zhu S

    更新日期:2019-09-01 00:00:00

  • Correlation between oxidative stress and the NF-κB signaling pathway in the pulmonary tissues of obese asthmatic mice.

    abstract::The obesity-asthma phenotype is characterized by increased asthma severity and decreased glucocorticoid responsiveness. To date, the mechanism underlying the association between obesity and asthma remain to be fully elucidated. The present study investigated the correlation between oxidative stress and the nuclear fac...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4663

    authors: Liu X,Lin R,Zhao B,Guan R,Li T,Jin R

    更新日期:2016-02-01 00:00:00

  • Nuclear respiratory factor 1 overexpression attenuates anti-benzopyrene‑7,8-diol-9,10-epoxide-induced S-phase arrest of bronchial epithelial cells.

    abstract::Nuclear respiratory factor 1 (NRF-1) has important roles in the regulation of several key metabolic genes required for cellular growth and respiration. A previous study by our group indicated that NRF‑1 is involved in mitochondrial dysfunction induced by the environmental pollutant benzo[a]pyrene in the 16HBE human br...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5065

    authors: Wu J,Wang Y,Wo D,Zhang L,Li J

    更新日期:2016-05-01 00:00:00

  • Prognostic relevance of the AQP5 -1364C>A polymorphism in primary breast cancer.

    abstract::Besides known prognostic factors in breast cancer, disseminated tumor cells are regarded as a surrogate marker for minimal residual disease in breast cancer. Their prognostic significance is comparable to that of lymph node status. However, the mechanism by which the primary tumor directs cells to disseminate into lym...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr_00000151

    authors: Kasimir-Bauer S,Heubner M,Otterbach F,Kimmig R,Siffert W,Adamzik M

    更新日期:2009-07-01 00:00:00

  • Differential effects of calcium antagonists on ABCG2/BCRP-mediated drug resistance and transport in SN-38-resistant HeLa cells.

    abstract::The effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport were examined in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP. Sensitivity to mitoxantrone, an ABCG2/BCRP substrate, in HeLa/SN100 cells was significantly reversed by ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2011.734

    authors: Takara K,Matsubara M,Yamamoto K,Minegaki T,Takegami S,Takahashi M,Yokoyama T,Okumura K

    更新日期:2012-03-01 00:00:00

  • Lentivirus transduced interleukin-1 receptor antagonist gene expression in murine bone marrow-derived mesenchymal stem cells in vitro.

    abstract::Genetically modified mesenchymal stem cells have been used in attempts to increase the expression of interleukin‑1 receptor antagonist (IL‑1Ra); however, the attempts thus far have been unsuccessful. The aim of the present study was to investigate whether the lentivirus transduced IL‑1Ra gene was able to be stably exp...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4003

    authors: He T,Chi G,Tian B,Tang T,Dai K

    更新日期:2015-09-01 00:00:00

  • Targeted next‑generation sequencing for research and diagnostics in congenital heart disease, and cleft lip and/or palate.

    abstract::Congenital heart disease (CHD), and cleft lip and palate (CLP) are currently the most common types of structural malformation in infants. Various methods have been used to identify the disease‑associated genes. However, targeted next‑generation sequencing (NGS) is not yet considered an option for routine use. Thus, th...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10043

    authors: Bu H,Liu L,Hu S,Tan Z,Zhao T

    更新日期:2019-05-01 00:00:00

  • Glucocorticoid treatment inhibits intracerebral hemorrhage‑induced inflammation by targeting the microRNA‑155/SOCS‑1 signaling pathway.

    abstract::Intracerebral hemorrhage (ICH) results in inflammation, and glucocorticoids have been proven to be effective inhibitors of ICH‑induced inflammation. However, the precise underlying mechanisms of ICH‑induced inflammation and glucocorticoid function remain largely undefined. Using a mouse ICH model, the present study de...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5716

    authors: Xu HF,Fang XY,Zhu SH,Xu XH,Zhang ZX,Wang ZF,Zhao ZQ,Ding YJ,Tao LY

    更新日期:2016-10-01 00:00:00

  • Hypoxia is important in F‑18 FDG accumulation in thecoma‑fibroma tumors on F‑18 FDG PET/CT scans.

    abstract::Several studies have noted benign thecoma‑fibroma tumors with positive F‑18 fluorodeoxyglucose (FDG) accumulation mimicking malignant ovarian tumors following F‑18 FDG positron emission tomography (PET). The present study analyzed four cases with false‑positive F‑18 FDG PET/computed tomography (CT) diagnoses of thecom...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5016

    authors: Seino H,Ono S,Miura H,Morohashi S,Wu Y,Tsushima F,Takai Y,Kijima H

    更新日期:2016-05-01 00:00:00

  • Gangliosides and CD82 inhibit the motility of colon cancer by downregulating the phosphorylation of EGFR at different tyrosine sites and signaling pathways.

    abstract::Previous studies have shown that (GM3), a ganglioside, suppresses hepatoma cell motility and migration by inhibiting phosphorylation of EGFR and the activity of the PI3K/AKT signaling pathway. Therefore, the aim of the present study was to investigate whether the combined treatment of CD82 with gangliosides can exert ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11467

    authors: Huang X,Li Y,He X,Chen Y,Wei W,Yang X,Ma K

    更新日期:2020-11-01 00:00:00

  • Circular RNA sequencing reveals the molecular mechanism of the effects of acupuncture and moxibustion on endometrial receptivity in patients undergoing infertility treatment.

    abstract::The present study aimed to determine the profile of differentially expressed circular RNAs (circRNAs) in infertile patients treated with acupuncture and moxibustion and verify the role of acupuncture and moxibustion in altering endometrial receptivity (ER). High‑throughput RNA sequencing and bioinformatics analysis of...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10386

    authors: Shen J,Chen L,Cheng J,Jin X,Mu Y,Li Q,Xia L,Gao Y,Xia Y

    更新日期:2019-08-01 00:00:00

  • CLI-095 decreases atherosclerosis by modulating foam cell formation in apolipoprotein E-deficient mice.

    abstract::Toll-like receptor 4 (TLR4) is considered to have a critical role in the occurrence and development of atherosclerosis in atherosclerosis-prone mice; however, it remains uncertain whether treatment with a TLR4 inhibitor may attenuate atherosclerosis. The present study aimed to determine the vascular protective effects...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5233

    authors: Wang XQ,Wan HQ,Wei XJ,Zhang Y,Qu P

    更新日期:2016-07-01 00:00:00

  • Graves' ophthalmopathy and tongue cancer complicated by peg-interferon α-2b and ribavirin therapy for chronic hepatitis C: A case report and review of the literature.

    abstract::Hepatitis C virus (HCV) infection induces not only chronic liver disease, but also extrahepatic manifestations such as thyroid disease and oral cancer. Thyroid dysfunction is also a complication known to be associated with interferon (IFN) therapy for HCV infection. We report on a 69-year-old Japanese man who develope...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr_00000003

    authors: Nagao Y,Hiromatsu Y,Nakashima T,Sata M

    更新日期:2008-09-01 00:00:00

  • Berberine inhibits the proliferation of prostate cancer cells and induces G₀/G₁ or G₂/M phase arrest at different concentrations.

    abstract::Prostate cancer is the second most common disease of the male reproductive system. Berberine is a quaternary ammonium salt that is extracted from plants. The aim of the current study was to explore the antitumor activity of berberine in prostate cancer cells and identify the underlying mechanism of its effects. PC3 hu...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.3139

    authors: Lu W,Du S,Wang J

    更新日期:2015-05-01 00:00:00

  • Role of KIF2A in the progression and metastasis of human glioma.

    abstract::KIF2A is a member of the kinesin-13 family and is involved in cell mitosis and spindle assembly. The abnormal expression and dysfunction of KIF2A are associated with tumorigenesis and the progression of certain human cancer types; however, little is known about the expression and roles of KIF2A in glioma. In the prese...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4700

    authors: Zhang X,Ma C,Wang Q,Liu J,Tian M,Yuan Y,Li X,Qu X

    更新日期:2016-02-01 00:00:00

  • Lentivirally overexpressed T-bet regulates T-helper cell lineage commitment in chronic hepatitis B patients.

    abstract::Chronic hepatitis B virus (HBV) infection is commonly considered to occur as a result of disturbance of the immune system. T-box expressed in T cells (T-bet) is an essential transcription factor for T helper (Th) cell differentiation and function. The aim of this study was to investigate the effect of T-bet overexpres...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.905

    authors: Liu X,Tang Z,Zhang Y,Hu J,Li D,Zang G,Yu Y

    更新日期:2012-08-01 00:00:00

  • Antihypertensive therapy improves insulin resistance and serum levels of interleukin-6 and -10 in spontaneously hypertensive rats with steatohepatitis.

    abstract::Metabolic syndrome based on insulin resistance (IR) and hypertension is a risk factor for advanced liver disease and cardiovascular disease in patients with nonalcoholic steatohepatitis (NASH). The present study investigated the effects of severe hypertension induced by a high‑salt (HS) diet and antihypertensive thera...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5875

    authors: Kozono M,Uto H,Ibusuki R,Arima S,Oda K,Taguchi H,Sasaki F,Nasu Y,Hashimoto S,Setoyama H,Kanmura S,Numata M,Tsubouchi H,Ido A

    更新日期:2016-12-01 00:00:00

  • Flow control effect of necrostatin-1 on cell death of the NRK-52E renal tubular epithelial cell line.

    abstract::Apoptosis and necroptosis occur in renal tubular epithelial cell (RTEC) death in acute kidney injury (AKI), and may be regulated by several methods. The present study identified a protective effect of necrostatin‑1 (Nec‑1) on RTECs via a flow-control-like effect. The results established a hypoxic‑ischemic injury model...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6556

    authors: Luo J,Tao Y,Liang X,Chen Y,Zhang L,Jiang F,Liu S,Ye Z,Li Z,Shi W

    更新日期:2017-07-01 00:00:00