Detection and Genetic Characterization of Metallo-β-Lactamase IMP-1 and VIM-2 in Pseudomonas aeruginosa Strains From Different Hospitals in Kermanshah, Iran.

Abstract:

BACKGROUND:Pseudomonas aeruginosais a frequent nosocomial pathogen that causes severe diseases in many settings. Carbapenems, including meropenem and imipenem, are effective antibiotics against this organism. However, the use of carbapenems has been hampered by the emergence of strains resistant to carbapenemsvia different mechanisms such as the production of metallo-β-lactamases (MBLs), which hydrolyze all carbapenems. Several kinds of MBLs have been reported, among them VIM and IMP types being the most clinically significant carbapenemases. OBJECTIVES:We aimed to determine the distribution of bla VIM-2 and bla IMP-1 transferable genes encoding MBLs in P. aeruginosa isolated from three academic hospitals in Kermanshah. PATIENTS AND METHODS:From 22nd June to 22nd September 2012, 225 isolates of P. aeruginosa were collected. These isolates were tested for antibiotic susceptibility with the Kirby-Bauer disk-diffusion method, and the MBLs were assessed using the imipenem-EDTA double-disk synergy test. The isolates were investigated for bla VIM - 2 and bla IMP-1 genes using polymerase chain reaction. RESULTS:Among the 225 isolates, 33.7% (76/225) and 18.1% (41/225) were resistant to imipenem and meropenem, respectively. Of the 76 imipenem-resistant P. aeruginosa strains, 45 (59.2%) were positive for MBLs, 34 (75%) strains carried the bla IMP-1 gene, and 1 (2.2%) strain carried the bla VIM - 2 gene. CONCLUSIONS:Our results showed that there was a high frequency of IMP-1 positive P. aeruginosa in the different wards of the hospitals.

authors

Abiri R,Mohammadi P,Shavani N,Rezaei M

doi

10.5812/jjm.22582

subject

Has Abstract

pub_date

2015-09-22 00:00:00

pages

e22582

issue

9

eissn

2008-3645

issn

2008-4161

journal_volume

8

pub_type

杂志文章
  • Designing and Construction Pcdna3.1 Vector Encoding Cfp10 Gene of Mycobacterium tuberculosis.

    abstract:BACKGROUND:Pathogenic mycobacteria are a major cause of human morbidity and mortality. Mycobacterium tuberculosis is an etiological agent of human tuberculosis (TB). Designing new vaccines, including DNA vaccines, may be a useful strategy for preventing TB. OBJECTIVES:The purpose of this study was to design and constr...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.23560

    authors: Baghani A,Youssefi M,Safdari H,Teimourpour R,Meshkat Z

    更新日期:2015-10-26 00:00:00

  • Expression Profiles of TGF-β and TLR Pathways in Porphyromonas gingivalis and Prevotella intermedia Challenged Osteoblasts.

    abstract:BACKGROUND:The presence of certain oral pathogens at implant sites can hinder the osseointegration process. However, it is unclear how and by what microorganisms it happens. OBJECTIVES:This study investigated whether the presence of oral pathogens of Porphyromonas gingivalis and Prevotella intermedia individually, pla...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.8(4)2015.17920

    authors: Aydin K,Ekinci FY,Korachi M

    更新日期:2015-04-18 00:00:00

  • New Insights into Sepsis Therapy Using Sepia Officinalis.

    abstract:BACKGROUND:Sepsis remains a major problem for both scientists and clinicians. Cecal ligation and puncture (CLP) is considered the gold standard for animal models of sepsis. The undesirable side effects of certain antibiotics have forced scientists to discover new, natural, and safe antimicrobial agents, such as cephalo...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.29331

    authors: Soliman AM,Fahmy SR,Sayed AA,Abd El-Latif AA

    更新日期:2016-01-02 00:00:00

  • Seroprevalence of Hepatitis A and E Virus Infections Among Healthy Population in Shiraz, Southern Iran.

    abstract:BACKGROUND:Enterically-transmitted acute viral hepatitis is caused predominantly by hepatitis A virus (HAV) and hepatitis E virus (HEV). The prevalence of HEV and HAV infections varies in different geographical regions. OBJECTIVES:This study was conducted to determine the prevalence of HEV and HAV infections among Ira...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.19311v2

    authors: Asaei S,Ziyaeyan M,Moeini M,Jamalidoust M,Behzadi MA

    更新日期:2015-07-27 00:00:00

  • Severe falciparum malaria in iran: a very rare case from an endemic region.

    abstract:INTRODUCTION:Malaria is a protozoal disease, transmitted to humans by female Anopheles mosquito bite. Plasmodium falciparum, compared to other kinds of Plasmodium, causes more severe malaria and is associated with a higher mortality rate. Annually, one to three million deaths occur due to malaria, especially by P. falc...

    journal_title:Jundishapur journal of microbiology

    pub_type:

    doi:10.5812/jjm.8752

    authors: Ziaee M,Abedi F

    更新日期:2014-01-01 00:00:00

  • The cutaneous bacterial microflora of the bodybuilders using anabolic-androgenic steroids.

    abstract:BACKGROUND:Anabolic-androgenic steroids (AAS) abuse by the athletes has dramatically increased during the recent decades. These substances might increase the skin lipids and enhance the cutaneous microbial proliferation. OBJECTIVES:The current study aimed to investigate the potential side effects of AAS on the bacteri...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.12269

    authors: Zomorodian K,Rahimi MJ,Taheri M,Ghanbari Asad A,Khani S,Ahrari I,Pakshir K,Khashei R

    更新日期:2014-12-06 00:00:00

  • Survival rates of human immunodeficiency virus and tuberculosis co-infected patients.

    abstract:BACKGROUND:At present, limited clinical data is available regarding survival rates of patients co-infected with human immunodeficiency virus (HIV)/tuberculosis (TB) in developing countries. OBJECTIVES:The present study aimed to evaluate the effect of HIV infection on the survival chances of active TB adults who disclo...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.10565

    authors: Roshanaei G,Sabouri Ghannad M,Saatchi M,Khazaei S,Mirzaei M

    更新日期:2014-06-01 00:00:00

  • Detection of algD, oprL and exoA Genes by New Specific Primers as an Efficient, Rapid and Accurate Procedure for Direct Diagnosis of Pseudomonas aeruginosa Strains in Clinical Samples.

    abstract:BACKGROUND:Pseudomonas aeruginosa is an opportunistic pathogen that infects patients with cystic fibrosis, burning wounds, ophthalmic traumas and immunodeficiency. OBJECTIVES:The aim of the present study was to compare the efficiency of newly designed primer sets with some previously published primers for PCR detectio...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.13583

    authors: Rashno Taee S,Khansarinejad B,Abtahi H,Najafimosleh M,Ghaznavi-Rad E

    更新日期:2014-10-01 00:00:00

  • Effect of Propranolol Alone and in Combination with Pyrimethamine on Acute Murine Toxoplasmosis.

    abstract:BACKGROUND:Toxoplasmosis is a public health problem worldwide. This complication principally affects immunodeficient patients and pregnant women. Toxoplasma gondii is an opportunistic parasite, causing severe illness among and death of high-risk individuals and treatment is becoming increasingly difficult owing to side...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.22572

    authors: Montazeri M,Daryani A,Ebrahimzadeh M,Ahmadpour E,Sharif M,Sarvi S

    更新日期:2015-09-07 00:00:00

  • Yeast colonization and drug susceptibility pattern in the pediatric patients with neutropenia.

    abstract:BACKGROUND:Pediatric patients with neutropenia are vulnerable to invasive Candida infections. Candida is the primary cause of fungal infections, particularly in immunosuppressed patients. Candida albicans has been the most common etiologic agent of these infections, affecting 48% of patients. OBJECTIVES:The aim of thi...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.11858

    authors: Haddadi P,Zareifar S,Badiee P,Alborzi A,Mokhtari M,Zomorodian K,Pakshir K,Jafarian H

    更新日期:2014-09-01 00:00:00

  • Antibiotic Susceptibility and mecA Frequency in Staphylococcus epidermidis, Isolated From Intensive Care Unit Patients.

    abstract:BACKGROUND:Coagulase-negative staphylococci (CoNS), especially Staphylococcus epidermidis, are considered as normal flora of human epithelia and also important opportunistic pathogens for nosocomial infections. S. epidermidis can also act as a reservoir for mecA, responsible for high-level resistance to methicillin and...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.11188

    authors: Najar-Peerayeh S,Jazayeri Moghadas A,Behmanesh M

    更新日期:2014-08-01 00:00:00

  • Antibiotics use patterns for surgical prophylaxis site infection in different surgical wards of a teaching hospital in ahvaz, iran.

    abstract:BACKGROUND:Despite the effectiveness of prophylactic antimicrobials to prevent surgical site infection the use of antibiotic prophylaxis is often inappropriate. OBJECTIVES:The current study aimed to determine the pattern of prophylactic antibiotic use in a teaching hospital affiliated to Jundishapur University of Medi...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.12251

    authors: Alavi SM,Roozbeh F,Behmanesh F,Alavi L

    更新日期:2014-11-01 00:00:00

  • Diagnostic Value of Serum Adenosine Deaminase (ADA) Level for Pulmonary Tuberculosis.

    abstract:BACKGROUND:Diagnosis of tuberculosis (TB) is not always easy, thus employing methods with a short duration and acceptable sensitivity and specificity is necessary to diagnose TB. OBJECTIVES:The aim of this study was to investigate the diagnostic value of serum adenosine deaminase (ADA) level for diagnosis of pulmonary...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.21760

    authors: Salmanzadeh S,Tavakkol H,Bavieh K,Alavi SM

    更新日期:2015-03-21 00:00:00

  • Increasing Prevalence of Aminoglycoside-Resistant Enterococcus faecalis Isolates Due to the aac(6')-aph(2") Gene: A Therapeutic Problem in Kermanshah, Iran.

    abstract:BACKGROUND:Enterococci are important pathogens in nosocomial infections. Various types of antibiotics, such as aminoglycosides, are used for treatment of these infections. Enterococci can acquire resistant traits, which can lead to therapeutic problems with aminoglycosides. OBJECTIVES:This study was designed to identi...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.28923

    authors: Khani M,Fatollahzade M,Pajavand H,Bakhtiari S,Abiri R

    更新日期:2016-03-12 00:00:00

  • Antimicrobial activity of seven essential oils from Iranian aromatic plants against common causes of oral infections.

    abstract:BACKGROUND:Over the past two decades, there has been a growing trend in using oral hygienic products originating from natural resources such as essential oils (EOs) and plant extracts. Seven aromatic plants used in this study are among popular traditional Iranian medicinal plants with potential application in modern me...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.17766

    authors: Zomorodian K,Ghadiri P,Saharkhiz MJ,Moein MR,Mehriar P,Bahrani F,Golzar T,Pakshir K,Fani MM

    更新日期:2015-02-19 00:00:00

  • Cloning and Expression of Poly 3-Hydroxybutyrate Operon Into Escherichia coli.

    abstract:BACKGROUND:Poly 3-Hydroxybutyrate (PHB), a class of Poly Hydroxyalkanoates (PHAs), is a group of bacterial storage polymers, produced by various microorganisms in response to nutrient limitation. PHAs are biodegradable polymers which could be a good substitute for current petrochemical plastics. PHB has been synthesize...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.16318

    authors: Jari M,Khatami SR,Galehdari H,Shafiei M

    更新日期:2015-02-20 00:00:00

  • Detection of Epstein-Barr Virus DNA in Langerhans Cell Histiocytosis.

    abstract:BACKGROUND:Langerhans cell histiocytosis (LCH) is a rare histiocytic proliferation of unknown etiology. It is characterized by granuloma-like proliferation of Langerhans-type dendritic cells and mainly affects young children. Although multiple investigators have suggested the possible role of viruses, such as Epstein-B...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.27219

    authors: Khoddami M,Nadji SA,Dehghanian P,Vahdatinia M,Shamshiri AR

    更新日期:2015-12-26 00:00:00

  • The impact of acute brucellosis on mean platelet volume and red blood cell distribution.

    abstract:BACKGROUND:Brucellosis is an inflammatory disease which may infect any organs or systems in the body. Mean Platelet Volume (MPV) is one of the most frequently used surrogate markers of platelet function. It reveals the presence of disease activity in many inflammatory diseases. Red blood cell distribution width (RDW) i...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.20039

    authors: Togan T,Narci H,Turan H,Ciftci O,Kursun E,Arslan H

    更新日期:2015-02-20 00:00:00

  • The Association Between Viral Infections and Co-stimulatory Gene Polymorphisms in Kidney Transplant Outcomes.

    abstract:BACKGROUND:The surveillance of kidney transplant patients depends on function of different immunologic markers like co-stimulatory molecules. These molecules may also be associated with post kidney transplant viral related outcomes. OBJECTIVES:The aim of this study was to investigate the possible associations between ...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.31338

    authors: Niknam A,Karimi MH,Yaghobi R,Geramizadeh B,Roozbeh J,Salehipour M,Iravani M

    更新日期:2016-02-17 00:00:00

  • Evaluation of Gastric Lesions Based on Helicobacter pylori and Helicobacter-Like Organisms (HLOs) in Cats; A Histopathological and Bacteriological Study.

    abstract:BACKGROUND:The lesions induced by Helicobacter pylori in a candidate animal model should always be examined thoroughly. The resemblance of these lesions to those observed in humans can indicate whether the usage of this model will contribute to the understanding of the various pathogenic mechanisms involved in the deve...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.9129

    authors: Sasani F,Javanbakht J,Kabir FR,Agha Mohammad Hassan M,Pashaei AR

    更新日期:2014-06-01 00:00:00

  • Isolation and Characterization of a Novel Magnetotactic Bacterium From Iran: Iron Uptake and Producing Magnetic Nanoparticles in Alphaproteobacterium MTB-KTN90.

    abstract:BACKGROUND:Magnetotactic bacteria (MTB) have the ability to biomineralize unique intracellular magnetic nanosize particles. These bacteria and their magnetosomes are under special attraction because of their great useful potential in nano-biotechnological and biomedical applications. MTB are ubiquitous in aquatic envir...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.19343

    authors: Tajer Mohammad Ghazvini P,Kermanshahi RK,Nozad Golikand A,Sadeghizadeh M

    更新日期:2014-09-01 00:00:00

  • Detection and Typing of Human Papilloma Viruses by Nested Multiplex Polymerase Chain Reaction Assay in Cervical Cancer.

    abstract:BACKGROUND:Cervical cancer is the leading cause of death from cancer in under-developed countries. Human papilloma virus (HPV) 16 and 18 are the most prevalent types associated with carcinogenesis in the cervix. Conventional Polymerase Chain Reaction (PCR), type-specific and consensus primer-based PCR followed by seque...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.26441

    authors: Jalal Kiani S,Shatizadeh Malekshahi S,Yousefi Ghalejoogh Z,Ghavvami N,Shafiei Jandaghi NZ,Shahsiah R,Jahanzad I,Yavarian J

    更新日期:2015-12-26 00:00:00

  • Phylogenetic and Transcripts Profiling of Glucose Sensing Related Genes in Candida glabrata.

    abstract:BACKGROUND:The sensing mechanism of glucose in Saccharomyces cerevisiae is well studied. However, such information is scarcely found in other yeast species such as Candida glabrata. OBJECTIVES:This study aimed to identify the glucose sensing pathway related genes of C. glabrata and to analyze the regulation pattern of...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.25177

    authors: Ng TS,Mohd Desa MN,Sandai D,Chong PP,Than LT

    更新日期:2015-11-21 00:00:00

  • Correlation of Multi-drug Resistance, Integron and blaESBL Gene Carriage With Genetic Fingerprints of Extended-Spectrum β-Lactamase Producing Klebsiella pneumoniae.

    abstract:BACKGROUND:Some genetic and phenotypic variables are associated among distinct microbial populations. OBJECTIVES:The associations between multi-drug resistance (MDR) phenotypes, prevalence of antibiotic resistance integrons (ARIs), bla SHV, bla TEM and bla CTX-M gene carriage and genetic fingerprints of random amplifi...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.8747

    authors: Ashayeri-Panah M,Feizabadi MM,Eftekhar F

    更新日期:2014-02-01 00:00:00

  • A Molecular Biological and Biochemical Investigation on Mycobacterium tuberculosis MutT Protein.

    abstract:BACKGROUND:Mycobacterium tuberculosis is a vicious microbe co-existing with the infected host. This pathogen exploited opportunities to spread during periods of urbanization and social upheaval, and got retreated with improved hygiene. OBJECTIVES:This investigation was designed to clone and characterize M. tuberculosi...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.9367

    authors: Huang HL,Su HT,Wu CH,Tsai-Wu JJ

    更新日期:2014-03-01 00:00:00

  • The Prevalence of Endocervical Chlamydia trachomatis Infection Among Young Females in Kashan, Iran.

    abstract:BACKGROUND:Chlamydia trachomatis is one of the major bacterial agents of the sexually transmitted diseases worldwide, especially among young females. There is no data regarding the prevalence of genital Chlamydia infection among young females in Kashan, Iran. OBJECTIVES:The current study aimed to determine the prevale...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.8(4)2015.15576

    authors: Afrasiabi S,Moniri R,Samimi M,Khorshidi A,Mousavi SG

    更新日期:2015-04-18 00:00:00

  • Comparison of Serum Hepatitis B Virus DNA and HBsAg Levels Between HBeAg-Negative and HBeAg-Positive Chronic Hepatitis B Patients.

    abstract:BACKGROUND:Chronic hepatitis B consists of different clinical phases. Laboratory and histological assessments can help differentiate the clinical phases of this disease and thus lead to better management. OBJECTIVES:This study was conducted to determine laboratory and histological characteristics of HBeAg-negative and...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.21444

    authors: Keshvari M,Alavian SM,Sharafi H

    更新日期:2015-01-14 00:00:00

  • Isolation, Identification and Evaluation of Antimicrobial Activity of Streptomyces flavogriseus, strain ACTK2 from Soil Sample of Kodagu, Karnataka State (India).

    abstract:BACKGROUND:The search for novel antibiotics continues to be of immense importance in research programs around the world for pharmaceutical, industrial and agricultural applications. Filamentous soil bacteria, belonging to the Streptomyces genus, are widely used as an important biological tool for their ability to produ...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.15107

    authors: Khandan Dezfully N,Gottravalli Ramanayaka J

    更新日期:2015-02-10 00:00:00

  • Multidrug-Resistant Escherichia coli and Klebsiella pneumoniae Isolated From Patients in Kashan, Iran.

    abstract:BACKGROUND:Escherichia coli and Klebsiella pneumoniae are common human pathogens that cause a wide spectrum of infections. Antimicrobial resistance is a basic obstacle in the management of these infections which has different patterns in various regions. OBJECTIVES:In this study, the antibiotic resistance patterns and...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.27517

    authors: Moini AS,Soltani B,Taghavi Ardakani A,Moravveji A,Erami M,Haji Rezaei M,Namazi M

    更新日期:2015-10-25 00:00:00

  • Detection of 23SrRNA Mutations Strongly Related to Clarithromycin Resistance in Helicobacter pylori Strains Isolated From Patients in the North of Iran.

    abstract:BACKGROUND:Helicobacter pylori is curved Gram negative and microaerophilic bacilli that have infected half of the world's population. It is recognized as the causative agent of duodenal ulcer, gastritis peptic ulcer, mucosa-associated lymphoid tissue (MALT) lymphoma and is associated with gastric adenocarcinoma. Resist...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.29694

    authors: Eghbali Z,Mojtahedi A,Moien Ansar M,Fakhrieh Asl S,Aminian K

    更新日期:2016-02-15 00:00:00