Donor's age and replicative senescence favour the in-vitro mineralization potential of human fibroblasts.


:Aberrant mineralization of soft connective tissues (ectopic calcification) may occur as a frequent age-related complication. Still, it remains unclear the role of mesenchymal cell donor's age and of replicative senescence on ectopic calcification. Therefore, the ability of cells to deposit in-vitro hydroxyapatite crystals and the expression of progressive ankylosis protein homolog (ANKH), ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), tissue non specific alkaline phosphatase (TNAP) and osteopontin (OPN) have been evaluated in human dermal fibroblasts derived from neonatal (nHDF) and adult (aHDF) donors (ex-vivo ageing model) or at low and high cumulative population doublings (CPD) up to replicative senescence (in-vitro ageing model). This study demonstrates that: 1) replicative senescence favours hydroxyapatite formation in cultured fibroblasts; 2) donor's age acts as a major modulator of the mineralizing potential of HDF, since nHDF are less prone than aHDF to induce calcification; 3) donor's age and replicative senescence play in concert synergistically increasing the calcification process; 4) the ANKH+ENPP1/TNAP ratio, being crucial for pyrophosphate/inorganic phosphate balance, is greatly influenced by donor's age, as well as by replicative senescence, and regulates mineral deposition; 5) OPN is only modulated by replicative senescence.


Exp Gerontol


Experimental gerontology


Boraldi F,Bartolomeo A,Di Bari C,Cocconi A,Quaglino D




Has Abstract


2015-12-01 00:00:00












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    abstract::Previous studies have demonstrated that fecundity, like mortality, plateaus at late ages in cohorts of Drosophila melanogaster. Although evolutionary theory can explain the decline and plateau in cohort fecundity at late ages, it is conceivable that lifelong heterogeneity in individual female fecundity is producing th...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Rauser CL,Abdel-Aal Y,Shieh JA,Suen CW,Mueller LD,Rose MR

    更新日期:2005-08-01 00:00:00

  • Aging and the neurocytoskeleton.

    abstract::It has been often demonstrated that during senescence some neurons undergo atrophic changes while others add new processes and terminals. Because microtubules form a substantial component of the dendritic and axonal cytoskeleton, we have studied the amount of tubulin and acetylated alpha-tubulin in three young (6 mont...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Fifkova E,Morales M

    更新日期:1992-01-01 00:00:00

  • Myeloid-derived suppressor cells promote age-related increase of lung cancer growth via B7-H1.

    abstract::Accumulation of myeloid-derived suppressor cells (MDSCs) in aged hosts contribute to the age-related increase of susceptibility to murine breast adenocarcinoma and co-stimulatory molecules expressed in MDSCs are essential for MDSCs-mediated immune suppression. However, the co-stimulatory molecules that exert a direct ...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Chen S,Liu H,Su N,Zhang G,Wang L

    更新日期:2015-01-01 00:00:00

  • Association of increased autophagic inclusions labeled for beta-galactosidase with fibroblastic aging.

    abstract::Replicative senescence appears after a finite number of cell divisions. After proliferation has ceased, senescent cells remain viable for long periods and metabolic modifications are observed such as lipofuscin accumulation. In order to understand this phenomenon, we examined the emergence of subcellular modifications...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Gerland LM,Peyrol S,Lallemand C,Branche R,Magaud JP,Ffrench M

    更新日期:2003-08-01 00:00:00

  • Neuromuscular electrical stimulation can improve mobility in older adults but the time course varies across tasks: Double-blind, randomized trial.

    abstract::Declines in mobility with advancing age are often associated with a reduction in the use of lower leg muscles. We examined the influence of two interventions that involved neuromuscular electrical stimulation (NMES) applied to the triceps surae muscles on the mobility and muscle function of older adults. Thirty health...

    journal_title:Experimental gerontology

    pub_type: 杂志文章,随机对照试验


    authors: Mani D,Almuklass AM,Amiridis IG,Enoka RM

    更新日期:2018-07-15 00:00:00

  • A theoretical model of the evolution of actuarial senescence under environmental stress.

    abstract::Free-living organisms are exposed to a wide range of stressors, all of which can disrupt components of stress-related and detoxification physiology. The subsequent accumulation of somatic damage is widely believed to play a major role in the evolution of senescence. Organisms have evolved sophisticated physiological r...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Watson H,Cohen AA,Isaksson C

    更新日期:2015-11-01 00:00:00

  • Age associated decline in the conversion of leucine to β-Hydroxy-β-Methylbutyrate in rats.

    abstract:BACKGROUND:The loss of muscle mass is considered to be a major factor contributing to strength decline during aging. β-Hydroxy-β-Methylbutyrate (HMB), a metabolite of leucine has been shown to enhance muscle protein synthesis and attenuate loss of muscle mass by multiple pathways. However, the production and regulation...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Shreeram S,Ramesh S,Puthan JK,Balakrishnan G,Subramanian R,Reddy MT,Pereira SL

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    abstract::Atrophy of the thymus, the primary site of T lymphocyte generation, is a hallmark of the aging immune system. Age-associated thymic atrophy results in diminished output of new, naïve T cells, with immune sequelae that include diminished responses to novel pathogenic challenge and vaccines, as well as diminished tumor ...

    journal_title:Experimental gerontology

    pub_type: 杂志文章,评审


    authors: Cepeda S,Griffith AV

    更新日期:2018-05-01 00:00:00

  • Total and phosphorylated tau protein as biological markers of Alzheimer's disease.

    abstract::Advances in our understanding of tau-mediated neurodegeneration in Alzheimer's disease (AD) are moving this disease pathway to center stage for the development of biomarkers and disease modifying drug discovery efforts. Immunoassays were developed detecting total (t-tau) and tau phosphorylated at specific epitopes (p-...

    journal_title:Experimental gerontology

    pub_type: 杂志文章,评审


    authors: Hampel H,Blennow K,Shaw LM,Hoessler YC,Zetterberg H,Trojanowski JQ

    更新日期:2010-01-01 00:00:00

  • Enhanced capacitative calcium entry and sarcoplasmic-reticulum calcium storage capacity with advanced age in murine mesenteric arterial smooth muscle cells.

    abstract::Intracellular Ca(2+) signaling is important to perfusion pressure related arterial reactivity and to vascular disorders including hypertension, angina and ischemic stroke. We have recently shown that advancing-age leads to calcium signaling adaptations in mesenteric arterial myocytes from C57 BL/6 mice [Corsso, C.D., ...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Goyal R,Angermann JE,Ostrovskaya O,Buchholz JN,Smith GD,Wilson SM

    更新日期:2009-03-01 00:00:00

  • Evidence that V+ fibronectin, GFAP and S100 beta mRNAs are increased in the hippocampus of aged rats.

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    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Wagner AP,Reck G,Platt D

    更新日期:1993-03-01 00:00:00

  • Mechanical and non-mechanical functions of Dystrophin can prevent cardiac abnormalities in Drosophila.

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    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Taghli-Lamallem O,Jagla K,Chamberlain JS,Bodmer R

    更新日期:2014-01-01 00:00:00

  • Memory performance of young and old subjects related to their erythrocyte characteristics.

    abstract::Several papers reported in recent years on a change in the age population distribution of the circulating erythrocytes in old mice, rats, rabbits, and humans. The results indicate the presence of a chronologically younger cell population in old animals and humans. The cells are typically lower in density and larger. I...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Danon D,Bologna NB,Gavendo S

    更新日期:1992-01-01 00:00:00

  • Proteomic analysis of protein complexes in human SH-SY5Y neuroblastoma cells by using blue-native gel electrophoresis: an increase in lamin A/C associated with heat shock protein 90 in response to 6-hydroxydopamine-induced oxidative stress.

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    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Nakamura M,Morisawa H,Imajoh-Ohmi S,Takamura C,Fukuda H,Toda T

    更新日期:2009-06-01 00:00:00

  • Neuron-specific overexpression of core clock genes improves stress-resistance and extends lifespan of Drosophila melanogaster.

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    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Solovev I,Dobrovolskaya E,Shaposhnikov M,Sheptyakov M,Moskalev A

    更新日期:2019-03-01 00:00:00

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    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Ferreira LB,de Nicola AC,Anselmo-Franci JA,Dornelles RC

    更新日期:2015-12-01 00:00:00

  • Mitochondrial efficiency is increased in axenically cultured Caenorhabditis elegans.

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    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Castelein N,Muschol M,Dhondt I,Cai H,De Vos WH,Dencher NA,Braeckman BP

    更新日期:2014-08-01 00:00:00

  • Age-related differences in body weight loss in response to altered thyroidal status.

    abstract::To determine whether age-related differences in body weight loss in hyperthyroidism could be related to caloric intake, the body weight and food consumption of Fischer 344 male rats were monitored every other day for four weeks. Six-month-old (young) rats were compared to 16-month-old rats (intermediate age) and 25-mo...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Mooradian AD

    更新日期:1990-01-01 00:00:00

  • Physical activity in midlife and telomere length measured in old age.

    abstract::Physical activity has been associated with alterations in telomere length, a potential indicator of biological aging, but several inconsistencies exist. Our aim was to investigate the associations between physical activity in midlife and leukocyte telomere length (LTL) measured in old age in the Helsinki Businessmen S...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Savela S,Saijonmaa O,Strandberg TE,Koistinen P,Strandberg AY,Tilvis RS,Pitkälä KH,Miettinen TA,Fyhrquist F

    更新日期:2013-01-01 00:00:00

  • Studies on the molecular-genetic basis of replicative senescence in Werner syndrome and normal fibroblasts.

    abstract::Based on evidence that human diploid fibroblasts (HDF) from the Werner syndrome (WS) of premature aging might overexpress an inhibitor of DNA synthesis (IDS), we prepared a eukaryotic cDNA expression library from WS mRNA and tested it for IDS activity in a transient assay. Two of six WS cDNA pools tested gave IDS acti...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Goldstein S,Murano S,Benes H,Moerman EJ,Jones RA,Thweatt R,Shmookler Reis RJ,Howard BH

    更新日期:1989-01-01 00:00:00

  • Effect of vitamin D3 supplementation and influence of BsmI polymorphism of the VDR gene of the inflammatory profile and oxidative stress in elderly women with vitamin D insufficiency: Vitamin D3 megadose reduces inflammatory markers.

    abstract:OBJECTIVE:This study aimed to evaluate the effect of vitamin D3 megadose supplementation and influence of BsmI polymorphism in the VDR gene on the inflammatory profile and oxidative stress in elderly women with vitamin D deficiency. METHODS:A double blind, randomized, placebo-controlled trial was conducted with 40 eld...

    journal_title:Experimental gerontology

    pub_type: 杂志文章,随机对照试验


    authors: de Medeiros Cavalcante IG,Silva AS,Costa MJ,Persuhn DC,Issa CT,de Luna Freire TL,da Conceição Rodrigues Gonçalves M

    更新日期:2015-06-01 00:00:00

  • CSF protein changes associated with hippocampal sclerosis risk gene variants highlight impact of GRN/PGRN.

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    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Fardo DW,Katsumata Y,Kauwe JSK,Deming Y,Harari O,Cruchaga C,Alzheimer's Disease Neuroimaging Initiative.,Nelson PT

    更新日期:2017-04-01 00:00:00

  • Diabetes, Alzheimer's disease and apolipoprotein genotype.

    abstract::Non-insulin dependent diabetes mellitus (NIDDM) has been associated with a number of physiological consequences including neuropathy, retinopathy and incidence of vascular disease. Recently, several authors reviewed studies that suggested that NIDDM is associated with cognitive impairments leading to a higher incidenc...

    journal_title:Experimental gerontology

    pub_type: 杂志文章,评审


    authors: Messier C

    更新日期:2003-09-01 00:00:00

  • Genetic variation for stress-response hormesis in C. elegans lifespan.

    abstract::Increased lifespan can be associated with greater resistance to many different stressors, most notably thermal stress. Such hormetic effects have also been found in C. elegans where short-term exposure to heat lengthens the lifespan. Genetic investigations have been carried out using mutation perturbations in a single...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Rodriguez M,Snoek LB,Riksen JA,Bevers RP,Kammenga JE

    更新日期:2012-08-01 00:00:00

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    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Nayak SB,Jena BS,Patnaik BK

    更新日期:1999-06-01 00:00:00

  • Vitamin E enhances the immune functions of young but not old mice under restraint stress.

    abstract::Young and old C57BL/6 male mice were given a diet containing a high dose of vitamin E (VE treatment) and its effect on the immune system was examined before and after the exposure to restraint stress. The VE treatment per se gave rise to a slight increase of splenic T cells in percentage and a significant enhancement ...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Wakikawa A,Utsuyama M,Wakabayashi A,Kitagawa M,Hirokawa K

    更新日期:1999-11-01 00:00:00

  • Re-expression of senescent markers in deinduced reversibly immortalized cells.

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    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Shay JW,West MD,Wright WE

    更新日期:1992-09-01 00:00:00

  • Practice effect and cueing of 2-minute walk test, 6-minute walk test and 10-meter walk test in frail older adults with and without dementia - Recommendations to walk tests protocols.

    abstract:OBJECTIVES:1) To determine if there was a practice effect associated with walk tests performed by frail older adults with and without dementia, 2) to examine the role of systematic cueing in the walk tests for those with dementia, and 3) to make recommendations to testing protocols of the walk tests for frail older adu...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Chan WLS,Pin TW

    更新日期:2019-09-01 00:00:00

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    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Felix ACS,Dutra SGV,Gastaldi AC,Bonfim PC,Vieira S,de Souza HCD

    更新日期:2018-08-01 00:00:00

  • Aging changes in the heart of a poeciliid fish, the guppy Poecilia reticulatus.

    abstract::Histological surveys of the hearts of guppies Poecilia reticulatus throughout their lifespan showed no aging changes until the fish were three years old. In younger fishes the hearts were healthy, even in three individuals with "wasting" disease, where there was considerable loss of muscle throughout the body. There w...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Woodhead AD

    更新日期:1984-01-01 00:00:00