Donor's age and replicative senescence favour the in-vitro mineralization potential of human fibroblasts.


:Aberrant mineralization of soft connective tissues (ectopic calcification) may occur as a frequent age-related complication. Still, it remains unclear the role of mesenchymal cell donor's age and of replicative senescence on ectopic calcification. Therefore, the ability of cells to deposit in-vitro hydroxyapatite crystals and the expression of progressive ankylosis protein homolog (ANKH), ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), tissue non specific alkaline phosphatase (TNAP) and osteopontin (OPN) have been evaluated in human dermal fibroblasts derived from neonatal (nHDF) and adult (aHDF) donors (ex-vivo ageing model) or at low and high cumulative population doublings (CPD) up to replicative senescence (in-vitro ageing model). This study demonstrates that: 1) replicative senescence favours hydroxyapatite formation in cultured fibroblasts; 2) donor's age acts as a major modulator of the mineralizing potential of HDF, since nHDF are less prone than aHDF to induce calcification; 3) donor's age and replicative senescence play in concert synergistically increasing the calcification process; 4) the ANKH+ENPP1/TNAP ratio, being crucial for pyrophosphate/inorganic phosphate balance, is greatly influenced by donor's age, as well as by replicative senescence, and regulates mineral deposition; 5) OPN is only modulated by replicative senescence.


Exp Gerontol


Experimental gerontology


Boraldi F,Bartolomeo A,Di Bari C,Cocconi A,Quaglino D




Has Abstract


2015-12-01 00:00:00












  • Renal function in familial longevity: the Leiden Longevity Study.

    abstract::Studying renal function in subjects with a familial propensity for longevity may provide insight in (un)known mechanisms that determine the age-related decline in renal function of normal subjects. In the Leiden Longevity Study, middle-aged offspring of non-agenarian siblings and their partners as environmentally matc...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: de Goeij MC,Halbesma N,Dekker FW,Wijsman CA,van Heemst D,Maier AB,Mooijaart SP,Slagboom PE,Westendorp RG,de Craen AJ

    更新日期:2014-03-01 00:00:00

  • Aging-related changes in human T-cell repertoire over 20years delineated by deep sequencing of peripheral T-cell receptors.

    abstract::Recent deep sequencing studies on T-cell receptor (TCR) repertoire have provided robust data to characterize diversity of T-cell immune responsiveness to a wide variety of peptide antigens, including viral and tumor antigens. The human TCR repertoire declines with age, but this decline has not been fully investigated ...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Yoshida K,Cologne JB,Cordova K,Misumi M,Yamaoka M,Kyoizumi S,Hayashi T,Robins H,Kusunoki Y

    更新日期:2017-10-01 00:00:00

  • Mitochondrial repair of 8-oxoguanine and changes with aging.

    abstract::Reactive oxygen species (ROS) are formed in all living organisms as a by-product of normal metabolism (endogenous sources) and as a consequence of exposure to environmental compounds (exogenous sources). Endogenous ROS are largely formed during oxidative phosphorylation in the mitochondria and, therefore, mitochondria...

    journal_title:Experimental gerontology

    pub_type: 杂志文章,评审


    authors: Stevnsner T,Thorslund T,de Souza-Pinto NC,Bohr VA

    更新日期:2002-10-01 00:00:00

  • Cardiac aging, calcium overload, and arrhythmias.

    abstract::The effect of aging was tested on experimental ventricular arrhythmias in isolated heart preparations from normal Wistar rats (NWR), Wistar Kyoto rats (WKY), and spontaneously hypertensive rats (SHR). Delayed afterdepolarizations and triggered activity induced by high-calcium perfusion (16 mM) in isolated papillary mu...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Carbonin PU,Di Gennaro M,Pahor M,Bernabei R,Sgadari A,Gambassi G Jr

    更新日期:1990-01-01 00:00:00

  • Preliminary studies of a novel bifunctional metal chelator targeting Alzheimer's amyloidogenesis.

    abstract::A growing body of evidence indicates that dysregulation of cerebral biometals (Fe, Cu, Zn) and their interactions with APP and Abeta amyloid may contribute to the Alzheimer's amyloid pathology, and thus metal chelation could be a rational therapeutic approach for interdicting AD pathogenesis. However, poor target spec...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Dedeoglu A,Cormier K,Payton S,Tseitlin KA,Kremsky JN,Lai L,Li X,Moir RD,Tanzi RE,Bush AI,Kowall NW,Rogers JT,Huang X

    更新日期:2004-11-01 00:00:00

  • The IL-6 -174G>C polymorphism is associated with cardiovascular diseases and mortality in 80-year-old humans.

    abstract::Chronic low-grade elevations in circulating levels of interleukin (IL)-6 act as a marker of subclinical cardiovascular diseases (CVD) and provide an independent predictor of increased mortality in elderly populations. The purpose of the present study was to test the hypothesis that the IL-6 -174G>C promoter polymorphi...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Bruunsgaard H,Christiansen L,Pedersen AN,Schroll M,Jørgensen T,Pedersen BK

    更新日期:2004-02-01 00:00:00

  • Role of muscle power output as a mediator between gait variability and gait velocity in hospitalized older adults.

    abstract::Acute illness requiring hospitalization is a sentinel event leading to functional decline and frequently, long-term disability in older adults. Although functional decline has become a key outcome during and after hospitalization, there is currently no gold standard for measuring functional impairment. The purpose of ...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Sáez de Asteasu ML,Martínez-Velilla N,Zambom-Ferraresi F,Casas-Herrero Á,Ramirez-Vélez R,Izquierdo M

    更新日期:2019-09-01 00:00:00

  • Comparison of the level of mitochondrial DNA A3243G mutation in esophageal epithelium and myocardium from individuals of very advanced age.

    abstract::The A3243G mutation, one of the changes of human mitochondrial DNA (mtDNA) that accumulates in cells during aging, is a useful marker for investigating the aging of cells. We measured the mutation level of the mtDNA A3243G mutation using DNAs from two different tissues (esophageal epithelium and myocardium) from advan...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Harihara S,Nakamura K,Takubo K,Takeuchi F

    更新日期:2002-07-01 00:00:00

  • Analysis of the effect of membrane arachidonic acid concentration on modulation of glutamate release by interleukin-1: an age-related study.

    abstract::Aging is associated with a change in membrane composition that includes a decrease in membrane polyunsaturated fatty acids, including arachidonic acid, and an increase in membrane cholesterol. Alterations in membrane structure are likely to impact on transmitter release, which relies on the fusion of synaptic plasma a...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: McGahon B,Murray CA,Clements MP,Lynch MA

    更新日期:1998-06-01 00:00:00

  • Muscle mass and aerobic capacity in older women: Impact of regular exercise at middle age.

    abstract:BACKGROUND:The impact of regular exercise habits at middle-age on muscle mass and function at old age remains inconclusive. While regular exercise likely represents a primary source of health-enhancing physical activity (PA), the physical demand of occupation needs to be considered. Additionally, PA level at old age sh...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Edholm P,Veen J,Kadi F,Nilsson A

    更新日期:2021-01-28 00:00:00

  • Control of calcium channels in neuroblastoma cells (N1E-115).

    abstract::Neuroblastoma cells (N1E-115) were used as models of transient (T) and long-lasting (L) Ca++ channels. The whole cell version of the patch clamp technique was used to measure inward Ca++ currents, and the fluorescent indicator, Fura-2, was used to measure changes in intracellular Ca++. Cells were cultured and selected...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Pang PK,Wang R,Wu LY,Karpinski E,Shan J,Benishin CG

    更新日期:1990-01-01 00:00:00

  • Quantitation of sex-specific serum N-glycome changes in expression level during mouse aging based on Bionic Glycome method.

    abstract::Studying the changes of serum N-glycome during mouse aging is beneficial to explore the molecular basis behind the alterations reported in human. However, such studies remainscarce and lack some information such as sialylation due to the method limitation. Here, we introduced Bionic Glycome method to quantify the seru...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Han J,Pan Y,Qin W,Gu Y,Xu X,Zhao R,Sha J,Zhang R,Gu J,Ren S

    更新日期:2020-11-01 00:00:00

  • Age-related changes in the atrial muscarinic type 2 receptor and their effects on atrial fibrillation vulnerability in rabbits.

    abstract::Aging plays an important role in increased vulnerability to atrial fibrillation (AF). Mediated by activity at the muscarinic type 2 receptor (M2R), the parasympathetic nerve contributes to the onset of AF. The purpose of this study was to investigate whether aging changes the distribution of M2R in the atrial myocardi...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Yang YH,Zheng QS,Li J,Shang FJ,Liu T,Wang HT,Liu XT,Liu L

    更新日期:2009-09-01 00:00:00

  • Administration of estradiol and progesterone modulate the activities of antioxidant enzyme and aminotransferases in naturally menopausal rats.

    abstract::In aging tissues the oxidative stress increases due to decreased activity of antioxidant enzymes and proteolysis increases due to decreased activity of aminotransferases, which can be modified by hormonal replacement therapy (HRT). The aim of the present study was to determine the effect of HRT on the activities of an...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Moorthy K,Sharma D,Basir SF,Baquer NZ

    更新日期:2005-04-01 00:00:00

  • Age-dependent modifications of expression level of VEGF and its receptors in the inner ear.

    abstract::The mechanisms responsible for age-associated hearing loss are still incompletely characterized. In this study, we used a murine model of age-dependent hearing loss and evaluated whether this condition is associated with vascular modifications of the structures of the inner ear. We used old C57BL/6J mice that are affe...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Picciotti P,Torsello A,Wolf FI,Paludetti G,Gaetani E,Pola R

    更新日期:2004-08-01 00:00:00

  • Aging murine B cells have decreased class switch induced by anti-CD40 or BAFF.

    abstract::We previously demonstrated that in vitro stimulated splenic B cells from senescent mice are deficient in production of multiple class switch isotypes, class switch recombination (CSR), induction of the E2A-encoded transcription factor E47, and activation-induced cytidine deaminase (AID) which is necessary for CSR and ...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Frasca D,Riley RL,Blomberg BB

    更新日期:2007-03-01 00:00:00

  • The adrenocortical stress-response in the aged male rat: impairment of recovery from stress.

    abstract::To test if the adrenocortical axis of the rat loses sensitivity to negative feedback control during aging, we examined corticosterone secretion under basal, stressed and post-stress conditions in young and in aged Fisher male rats. We find no age-related change in the speed or magnitude of the stress response or in th...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Sapolsky RM,Krey LC,McEwen BS

    更新日期:1983-01-01 00:00:00

  • A light and electron microscope study of aging parotid and submandibular salivary glands of Swiss-Webster mice.

    abstract::Light and electron microscope comparisons were made of parotid and submandibular glands from male Swiss-Webster white mice 3, 13, and 18 months old. The glands from the 13- and 18-month-old mice were less organized and the parenchyma was not as dense. Fibrous connective tissue, intracellular lipofuscin granules, and r...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Meisel DL,Skobe Z,Prostak KS,Shklar G

    更新日期:1988-01-01 00:00:00

  • Aging and the neurocytoskeleton.

    abstract::It has been often demonstrated that during senescence some neurons undergo atrophic changes while others add new processes and terminals. Because microtubules form a substantial component of the dendritic and axonal cytoskeleton, we have studied the amount of tubulin and acetylated alpha-tubulin in three young (6 mont...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Fifkova E,Morales M

    更新日期:1992-01-01 00:00:00

  • Accelerated aging of fasted Drosophila. Preservation of physiological function and cellular fine structure by thiazolidine carboxylic acid (TCA).

    abstract::Thiazolidine carboxylic acid (TCA) is a natural liver metabolite whose Mg-salt increased lifespan of flies and mice (Miquel and Economos, 1979, Exp. Geront. 14: 279). We studied the physiological and cellular fine structural effects of various concentrations of TCA in the food of male Drosophila. Flies on 0.3% TCA at ...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Economos AC,Ballard RC,Miquel J,Binnard R,Philpott DE

    更新日期:1982-01-01 00:00:00

  • Aging and cancer mortality: dynamics of change and sex differences.

    abstract::Age-related changes in cancer mortality risk are important for understanding the processes of disease and aging interaction. The extent to which these age changes differ by sex further contributes to this understanding but has not been well studied to date. We conducted a systematic examination of dynamics and heterog...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Yang Y,Li T,Nielsen ME

    更新日期:2012-09-01 00:00:00

  • Age-dependent increase of indigenous DNA adducts in rat brain is associated with a lipid peroxidation product.

    abstract::Indigenous DNA adducts (I-compounds) are considered to be a biomarker of aging tissues. Thus far, few studies have been conducted to investigate the accumulation patterns of I-compounds in the brain during aging. Particularly, identities of age-dependent I-compounds have largely remained unknown. In the current study,...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Cai Q,Tian L,Wei H

    更新日期:1996-05-01 00:00:00

  • Longitudinal study of in vivo wound repair and in vitro cellular senescence of dermal fibroblasts.

    abstract::A longitudinal study was performed to confirm the inverse relationship between in vivo age and in vitro proliferative capacity previously observed in a cross-sectional study, and to investigate the relationship between the growth of dermal fibroblasts in vitro and a physiological function (i.e., wound repair) that is ...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Bruce SA,Deamond SF

    更新日期:1991-01-01 00:00:00

  • Demographic mechanisms for the evolution of long life in social insects.

    abstract::One of the most remarkable life history traits of many species of eusocial insects is the extraordinary longevity of their queens, with the recorded life span of some individuals exceeding 30 years. Surprisingly, little research has been conducted on either the proximate or ultimate questions concerning what factors f...

    journal_title:Experimental gerontology

    pub_type: 杂志文章,评审


    authors: Carey JR

    更新日期:2001-04-01 00:00:00

  • IL-6 is a predictive biomarker for stroke associated infection and future mortality in the elderly after an ischemic stroke.

    abstract:BACKGROUND AND PURPOSE:Stroke associated infection (within the first seven days) occurs in approximately half of stroke patients and is associated with a worse prognosis, especially in the elderly. It is uncertain what factors predict stroke associated infection, yet identification of a suitable biomarker for infection...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Kwan J,Horsfield G,Bryant T,Gawne-Cain M,Durward G,Byrne CD,Englyst NA

    更新日期:2013-09-01 00:00:00

  • Gene expression and generation of CD28-CD8 T cells mediated by interleukin 15.

    abstract::Accumulation of CD28(-)CD8 T cells that are defective in response to antigenic stimulation is a hallmark of age-associated decline in T cell function. However, the underlying mechanism of this age-associated change is not fully understood. We recently analyzed the global gene expression profiles of CD8 T cell subsets ...

    journal_title:Experimental gerontology

    pub_type: 杂志文章,评审


    authors: Godlove J,Chiu WK,Weng NP

    更新日期:2007-05-01 00:00:00

  • Metabolism in the Caenorhabditis elegans Mit mutants.

    abstract::In many eukaryotes oxidative phosphorylation via the mitochondrial electron transport chain provides the major means of ATP production. Complete removal of this capacity often results in premature death. Recent studies using the nematode Caenorhabditis elegans are surprising because they have revealed that disruption ...

    journal_title:Experimental gerontology

    pub_type: 杂志文章,评审


    authors: Rea SL

    更新日期:2005-11-01 00:00:00

  • A cluster analysis study of acetyl-L-carnitine effect on NMDA receptors in aging.

    abstract::Aging is associated with a reduction in the maximum density of n-methyl-d-aspartate (NMDA)-sensitive glutamate binding sites in the hippocampus of Fischer 344 rats. This study was designed to investigate the effect of acetyl-l-carnitine (ALCAR) on NMDA receptors in the old rat (24 months) after chronic or single-dose ...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: Castorina M,Ambrosini AM,Giuliani A,Pacifici L,Ramacci MT,Angelucci L

    更新日期:1993-11-01 00:00:00

  • Muscle fatigability measured with Pneumatic and Hydraulic handgrip systems are not interchangeable.

    abstract:BACKGROUND:Fatigue resistance (FR) was here defined as the time during which grip strength (GS) drops to 50% of its maximum during sustained contraction. Since different GS systems exist, we compared FR obtained with Pneumatic (Pneu) and Hydraulic (Hydr) handgrip systems. Hand pain induced by both systems was also inve...

    journal_title:Experimental gerontology

    pub_type: 杂志文章


    authors: De Dobbeleer L,Beckwée D,Arnold P,Baudry S,Beyer I,Demarteau J,Lieten S,Punie Y,Bautmans I

    更新日期:2020-07-15 00:00:00

  • SV40-mediated immortalization of human fibroblasts.

    abstract::We have identified a multistep mechanism by which the DNA virus SV40 overcomes cellular senescence. Expression of SV40 T antigen is required for both transient extension of life span and unlimited life span or immortalization. These effects are mediated through inactivation of function of growth suppressors pRB and p5...

    journal_title:Experimental gerontology

    pub_type: 杂志文章,评审


    authors: Ozer HL,Banga SS,Dasgupta T,Houghton J,Hubbard K,Jha KK,Kim SH,Lenahan M,Pang Z,Pardinas JR,Patsalis PC

    更新日期:1996-01-01 00:00:00