Blastocystis hominis: phylogenetic affinities determined by rRNA sequence comparison.


:In 1912 Blastocystis hominis was identified as a new species and classified as a yeast (Brumpt 1912). In the early 1920s several groups confirmed its classification as a yeast, specifically a member of the genus Schizosaccharomyces (discussed by Zierdt et al. 1967). Apart from an occasional case report, the classification of B. hominis and its role as a harmless intestinal yeast was not questioned for another 50 years. Then, Zierdt (1967) suggested that it should be classified in the phylum Protozoa, subphylum Sporozoa, and that it should be considered as a potential pathogen. The likely role of B. hominis as a human pathogen has recently become more firmly established (Garcia et al. 1984; Sheehan et al. 1986) and its classification has been changed. Although the classification of B. hominis as a protozoon was assumed widely, classification as a sporozoon was not accepted, and the most recent definitive classification of the Protozoa did not even list B. hominis (Lee et al. 1985). Then, based essentially on a review of the known characteristics of the organism, it was recently reclassified into the subphylum Sarcodina (Zierdt 1988). Clearly, the phylogeny of this emerging human pathogen needs definitive analysis (Mehlhorn 1988).


Exp Parasitol


Johnson AM,Thanou A,Boreham PF,Baverstock PR




Has Abstract


1989-04-01 00:00:00














  • Scanning electron microscopy of Haemonchus contortus exposed to tannin-rich plants under in vivo and in vitro conditions.

    abstract::The structural changes induced in adult Haemonchus contortus after in vitro and in vivo contact with a tannin-rich (TR) plant, either tzalam (Lysiloma latisiliquum) or sainfoin (Onobrychis viciifolia), were assessed using scanning electron microscopy (SEM). All the worms used in the study were adult females. The Haemo...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Martínez-Ortíz-de-Montellano C,Arroyo-López C,Fourquaux I,Torres-Acosta JF,Sandoval-Castro CA,Hoste H

    更新日期:2013-03-01 00:00:00

  • Development of a molecular xenomonitoring protocol to assess filariasis transmission.

    abstract::According to the World Health Organization, lymphatic filariasis (LF), a mosquito-borne neglected tropical disease (NTD), should be eliminated as a public health concern by the end of 2020. To this end, the goals of the Global Programme to Eliminate Lymphatic Filariasis (GPELF) include interrupting transmission throug...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Albuquerque AL,Araújo TA,Melo DCTV,Paiva MHS,Melo FL,Oliveira CMF,Ayres CFJ

    更新日期:2020-08-01 00:00:00

  • Entamoeba histolytica: the serine-rich gene polymorphism-based genetic variability of clinical isolates from Georgia.

    abstract::It is generally accepted that a majority of individuals infected by Entamoeba histolytica do not develop symptomatic disease. However, the parasite and the host factors contributing to the development of the disease, remain undetermined. It is also unclear why certain individuals develop extra-intestinal amebiasis wit...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Simonishvili S,Tsanava S,Sanadze K,Chlikadze R,Miskalishvili A,Lomkatsi N,Imnadze P,Petri WA Jr,Trapaidze N

    更新日期:2005-07-01 00:00:00

  • Trypanosoma brucei: two-dimensional gel analysis of the major glycosomal proteins during the life cycle.

    abstract::Kinetoplastid organisms possess a unique organelle, the glycosome, which compartmentalizes the Embden-Meyerhof segment of glycolysis and several other metabolic pathways. In Trypanosoma brucei many of the enzyme activities localized to the glycosome are stage regulated. Two-dimensional gel analysis was used to examine...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Parsons M,Nielsen B

    更新日期:1990-04-01 00:00:00

  • Plasmodium vivax: polymorphism in the merozoite surface protein 1 gene from wild Colombian isolates.

    abstract::The Plasmodium vivax merozoite surface protein-1 (PvMSP-1) has been considered a candidate for a malaria vaccine against erythrocytic stages. PvMSP-1 is immunogenic during natural infections and exhibits antigenic polymorphism. The extent of genetic polymorphism in a region between the so-called interspecies conserved...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Gutierrez A,Vicini J,Patarroyo ME,Murillo LA,Patarroyo MA

    更新日期:2000-07-01 00:00:00

  • Balamuthia and Acanthamoeba-binding antibodies in West African human sera.

    abstract::Little is known about the prevalence of Balamuthia mandrillaris amoebae and Balamuthia amoebic encephalitis in Africa. As an approach, relative concentrations of amoebae-binding serum antibodies (Ab) were assessed by flow cytometry using formaldehyde-fixed B. mandrillaris, Acanthamoeba lenticulata 72-2 and Acanthamoeb...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Kiderlen AF,Radam E,Schuster FL,Adjogoua EV,Akoua-Koffi C,Leendertz FH

    更新日期:2010-09-01 00:00:00

  • Trypanosoma cruzi: multiple actin isovariants are observed along different developmental stages.

    abstract::The expression and biological role of actin during the Trypanosoma cruzi life cycle remains largely unknown. Polyclonal antibodies against a recombinant T. cruzi actin protein were used to confirm its expression in epimastigotes, trypomastigotes, and amastigotes. Although the overall levels of expression were similar,...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Cevallos AM,Segura-Kato YX,Merchant-Larios H,Manning-Cela R,Alberto Hernández-Osorio L,Márquez-Dueñas C,Ambrosio JR,Reynoso-Ducoing O,Hernández R

    更新日期:2011-01-01 00:00:00

  • In vitro anti-leishmanial efficacy of potato tuber extract (PTEx): leishmanial serine protease(s) as putative target.

    abstract::Leishmaniasis, a neglected tropical disease (NTD) causes major health problems in the tropical and subtropical world. Most of the antileishmanial modern therapies with different formulations of pentavalent antimonials, Miltefosine, Amphotericin B etc. are not satisfactory in recent times due to high toxicity to the ho...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Paik D,Das P,De T,Chakraborti T

    更新日期:2014-11-01 00:00:00

  • ABO blood groups of residents and the ABO host choice of malaria vectors in southern Iran.

    abstract::Recent epidemiological evidences revealed the higher prevalence of 'O' blood group in the residents of malaria-endemic areas. Also some data indicated preference of mosquitoes to 'O' group. The aim of this study was to determine ABO group ratio in the residents as well as ABO group preference of Anopheles in two malar...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Anjomruz M,Oshaghi MA,Sedaghat MM,Pourfatollah AA,Raeisi A,Vatandoost H,Mohtarami F,Yeryan M,Bakhshi H,Nikpoor F

    更新日期:2014-01-01 00:00:00

  • Plasmodium falciparum: synchronization of asexual development with aphidicolin, a DNA synthesis inhibitor.

    abstract::The asexual development cycle of Plasmodium falciparum, a malarial parasite of humans, has been synchronized in culture by treating ring-stage parasites with aphidicolin, an inhibitor of DNA synthesis. Optimization of both the concentration of drug added to ring stage containing red blood cells and the duration of exp...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Inselburg J,Banyal HS

    更新日期:1984-02-01 00:00:00

  • Schistosoma mansoni: susceptibility differences between male and female mice can be mediated by testosterone during early infection.

    abstract::In murine Schistosoma mansoni infections, fewer adult worms develop in male than in female mice infected with the same number of cercariae. To evaluate a potential role for testosterone in this phenomenon, testosterone levels were manipulated in groups of CBA/J mice that were then infected and monitored for survival r...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Nakazawa M,Fantappie MR,Freeman GL Jr,Eloi-Santos S,Olsen NJ,Kovacs WJ,Secor WE,Colley DG

    更新日期:1997-03-01 00:00:00

  • Effects of age and splenectomy on heavy infection of Angiostrongylus cantonensis in rats.

    abstract::This study was designed to determine the effects of age and the role of spleen in rats with heavy Angiostrongylus cantonensis infection. Young rats (8 weeks) infected with 100 larvae were found to have significantly higher worm recovery rate (75.0±6.6%) than the adult (6 months) (55.7±1.5%) and the aging ones (13 mont...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Wang LC,Yen CM,Liu CL,Chen ER,Chao D

    更新日期:2012-06-01 00:00:00

  • Schistosoma mansoni: molecular characterization of Alkaline Phosphatase and expression patterns across life cycle stages.

    abstract::Here we describe the cloning and characterization of the Schistosoma mansoni Alkaline Phosphatase (SmAP), previously identified in the tegument of adult worms. SmAP encodes a complete sequence composed of 536 amino acids containing an N-terminal signal peptide, five N-glycosylation sites, and a GPI anchor signal, simi...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Araujo-Montoya BO,Rofatto HK,Tararam CA,Farias LP,Oliveira KC,Verjovski-Almeida S,Wilson RA,Leite LC

    更新日期:2011-11-01 00:00:00

  • Schistosoma mansoni: surface membrane stability in vitro and in vivo.

    abstract::The human complement component C3b is known to bind in vitro to the surfaces of all developmental stages of schistosomes as a consequence of complement activation by the alternative pathway. C3b bound to Schistosoma mansoni parasites has now been used in combination with fluorescent labeled antibodies against C3b to l...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Ruppel A,McLaren DJ

    更新日期:1986-10-01 00:00:00

  • Plasmodium vinckei: optimization of desferrioxamine B delivery in the treatment of murine malaria.

    abstract::Optimization of desferrioxamine B (DFO) delivery for the treatment of malaria was studied in Plasmodium vinckei infected mice. DFO was administered by three different treatment regimens: (1) multiple subcutaneous injections of free DFO, (2) intraperitoneal infusion of free DFO, or (3) multiple subcutaneous injections ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Postma NS,Hermsen CC,Zuidema J,Eling WM

    更新日期:1998-07-01 00:00:00

  • Susceptibility of Brazilian isolates of Trypanosoma evansi to suramin sodium: test in experimentally infected mice.

    abstract::This study aimed to evaluate the susceptibility of Brazilian isolates of Trypanosoma evansi to suramin sodium. For this purpose, three isolates of T. evansi (LPV-2005, LPV-2009 and LPV-2010) and seventy mice were used, with the animals divided in 10 groups (A, B, C, D, E, F, G, H, I and J) with seven animals each grou...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Faccio L,Da Silva AS,Gressler LT,Tonin AA,Lazzarotto CR,Miletti LC,Monteiro SG

    更新日期:2013-07-01 00:00:00

  • Exploration of fluorescence-based real-time loop-mediated isothermal amplification (LAMP) assay for detection of Isospora suis oocysts.

    abstract::Isospora suis is an intestinal protozoan parasite in pigs. The 2-3 weeks old piglets are most often infected by I. suis because their immune system is not fully developed. The infection exhibits clinical features such as diarrhea and dehydration and seriously affects the economic interests of farmers. The traditional ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Huang C,Wen F,Yue L,Chen R,Zhou W,Hu L,Chen M,Wang S

    更新日期:2016-06-01 00:00:00

  • Toxoplasma gondii: a simple high-throughput assay for drug screening in vitro.

    abstract::Toxoplasma gondii is the etiologic agent of toxoplasmosis. Although the combination of sulfadiazine and pyrimethamine is used as therapy for this disease, these drugs can have serious side effects and its use is limited in pregnancy. Therefore there is a need for new anti-T. gondii drugs in the clinic. Some systems fo...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Jin C,Kaewintajuk K,Jiang J,Jeong W,Kamata M,Kim HS,Wataya Y,Park H

    更新日期:2009-02-01 00:00:00

  • Tritrichomonas foetus: iron acquisition from lactoferrin and transferrin.

    abstract::Acquisition of iron from lactoferrin and transferrin by a parasitic protozoon Tritrichomonas foetus has been studied in vitro. Specific, time-dependent, and saturable binding of iodinated ligands to the outer membrane of T. foetus at 4 degrees C was demonstrated for 125I-labeled lactoferrin only. About 1.7 x 10(5) bin...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Tachezy J,Kulda J,Bahníková I,Suchan P,Rázga J,Schrével J

    更新日期:1996-07-01 00:00:00

  • Toxoplasma gondii: a simple Real-time PCR assay to quantify the proliferation of the apicoplast.

    abstract::A Real-time quantitative PCR assay to quantify the Toxoplasma gondii apicoplast was studied. Primers were designed to amplify a 305bp product specific to T. gondii apicoplast. Standard curves were generated for both T. gondii apicoplast DNA and genomic DNA, and were used to compute the relative concentration of apicop...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Wu L,Chen SX,Jiang XG,Cao JP

    更新日期:2009-12-01 00:00:00

  • Repellency effect of Pilocarpus spicatus A. St.-Hil essential oil and nanoemulsion against Rhipicephalus microplus larvae.

    abstract::Rhipicephalus microplus, the cattle tick, is a major cause of economic losses in bovine production. Due to the widespread acaricidal resistance to commercially available products, as well as their toxicity and environmental impact, alternative control methods are required. Nanoformulations produced from plant extracts...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Nogueira JAP,Figueiredo A,Duarte JL,de Almeida FB,Santos MG,Nascimento LM,Fernandes CP,Mourão SC,Toscano JHB,Rocha LM,Chagas ACS

    更新日期:2020-08-01 00:00:00

  • Toxicity effect of the acaricide fipronil in semi-engorged females of the tick Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae): preliminary determination of the minimum lethal concentration and LC(50).

    abstract::Chemical acaricides, especially fipronil (active ingredient of Frontline®), are still the most effective method to control tick populations. In this study, the effectiveness of fipronil was assessed in semi-engorged females of the tick Rhipicephalus sanguineus. A protocol for an in vitro bioassay (AIT) was developed, ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: de Oliveira PR,Bechara GH,Denardi SE,Pizano MA,Mathias MI

    更新日期:2011-02-01 00:00:00

  • Pfmdr1 Asn1042Asp and Asp1246Tyr polymorphisms, thought to be associated with chloroquine resistance, are present in chloroquine-resistant and -sensitive Brazilian field isolates of Plasmodium falciparum.

    abstract::Parasite resistance to antimalarial drugs, particularly chloroquine, is the most disturbing problem of malaria chemotherapy. There is evidence that the codon 86Tyr polymorphism of the Pfmdr1 gene is associated with chloroquine resistance in West African Plasmodium falciparum. The association of this and four other cod...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Póvoa MM,Adagu IS,Oliveira SG,Machado RL,Miles MA,Warhurst DC

    更新日期:1998-01-01 00:00:00

  • Changes in the lipid profile of Bradybaena similaris (Férussac, 1821) (Gastropoda, Xanthonychidae) during the development of Eurytrema coelomaticum (Giard and Billet, 1892) (Digenea, Dicrocoeliidae).

    abstract::The effect of experimental exposure of Bradybaena similaris by Eurytrema coelomaticum on the cholesterol and triglycerides total levels circulating in the hemolymph and the neutral lipids in the digestive gland-gonad (DGG) complex of the host snail were studied. In this study, snails were dissected after one, two, and...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Alves H,Tunholi-Alves VM,Tunholi VM,Gôlo P,Bittencourt VR,Pinheiro J

    更新日期:2014-09-01 00:00:00

  • A new Leishmania hypothetical protein can be used for accurate serodiagnosis of canine and human visceral leishmaniasis and as a potential prognostic marker for human disease.

    abstract::Distinct antigens have been evaluated with diagnostic purpose for canine and human visceral leishmaniasis (VL), and variable sensitivity and specificity values have been obtained in the assays. In the present study, a Leishmania infantum hypothetical protein called LiHyG, which was identified in an immunoproteomics st...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Machado AS,Ramos FF,Santos TTO,Costa LE,Ludolf F,Lage DP,Bandeira RS,Tavares GSV,Oliveira-da-Silva JA,Steiner BT,Chaves AT,Oliveira JS,Chávez-Fumagalli MA,de Magalhães-Soares DF,Silveira JAG,Duarte MC,Machado-de-Ávila RA

    更新日期:2020-09-01 00:00:00

  • Immunomodulatory effect of the alkaloidic extract of Solanum lycocarpum fruits in mice infected with Schistosoma mansoni.

    abstract::Schistosomiasis is a chronic disease caused by trematode flatworms of the genus Schistosoma; it accounts for more than 280,000 deaths annually. In this work we investigated the effect of the alkaloidic extract obtained by acid-base extraction of the dried fruits of Solanum lycocarpum on schistosomiasis. We used this e...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Miranda MA,Kuehn CC,Cardoso JF,Oliveira LG,Magalhães LG,Tiossi RF,Rodrigues V,Zucolloto S,Prado JC Jr,McChesney JD,Bastos JK

    更新日期:2013-04-01 00:00:00

  • Early Trichinella spiralis and Trichinella nativa infections induce similar gene expression profiles in rat jejunal mucosa.

    abstract::Trichinella spiralis causes a significantly higher parasite burden in rat muscle than Trichinella nativa. To assess whether the difference in infectivity is due to the early intestinal response, we analyzed gene expression changes in the rat jejunum during Trichinella infection with a whole-genome microarray. The rats...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Airas N,Näreaho A,Lindén J,Valo E,Hautaniemi S,Jokelainen P,Sukura A

    更新日期:2013-10-01 00:00:00

  • Echinostoma caproni: kinetics of IgM, IgA and IgG subclasses in the serum and intestine of experimentally infected rats and mice.

    abstract::The kinetics of specific immunoglobulin M, A and IgG subclasses against Echinostoma caproni (Trematoda: Echinostomatidae) were analyzed in serum and intestinal fluid of two host species (Wistar rats and ICR mice) in which the course of the infection markedly differs. In rats, the worms were rapidly expelled, whereas E...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Sotillo J,Muñoz-Antoli C,Marcilla A,Fried B,Guillermo Esteban J,Toledo R

    更新日期:2007-08-01 00:00:00

  • Brachymeria lasus: effects of nutrient level on in vitro larval growth of a chalcid insect parasite.

    abstract::Exponential weight gain equations and growth rates were determined during larval development of Brachymeria lasus reared aseptically on chemically defined media with varying amino acid and glucose levels. The rate of weight gain increased from 0 to 2% dietary glucose and mean growth rate increased from 160 to 260 micr...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Thompson SN

    更新日期:1983-06-01 00:00:00

  • Toxoplasma gondii: identification and immune response against a group of proteins involved in cellular invasion.

    abstract::Toxoplasma gondii is an ubiquitous intracellular parasite, causative agent of toxoplasmosis, and a worldwide zoonosis for which an effective vaccine is needed. A group of proteins secreted by tachyzoites during host-cell invasion was isolated from the interaction medium. It induced the permeability of the cells as ass...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Azzouz S,Maache M,Osuna A,Lawton P,Pétavy AF

    更新日期:2012-01-01 00:00:00