Culture expanded primary chondrocytes have potent immunomodulatory properties and do not induce an allogeneic immune response.


OBJECTIVE:Allogeneic cell therapies, such as mesenchymal stromal cells (MSC), which have potent regenerative and anti-inflammatory potential are being investigated as a therapy for osteoarthritis (OA) and cartilage injury. Here we describe another potential source of regenerative and anti-inflammatory allogeneic cells, culture expanded primary chondrocytes (CEPC). In direct comparison to allogeneic MSC, we extensively assess the immunological interactions of CEPC in an allogeneic setting. METHODS:Chondrocytes were isolated from rat articular cartilage and cultured in normoxic or hypoxic conditions. In vitro co-culture assays with allogeneic lymphocytes and macrophages were used to assess the immunomodulatory capacities of the chondrocytes, followed by immune response analysis by flow cytometry, ELISA and qPCR. RESULTS:CEPC showed reduced induction of proliferation, activation and cytotoxic granzyme B expression in allogeneic T cells. Importantly, exposure to pro-inflammatory cytokines did not increase CEPC immunogenicity despite increases in MHC-I. Furthermore, CEPC had a potent ability to suppress allogeneic T cell proliferation, which was dependent on nitric oxide production. This suppression was contact independent in hypoxia cultured CEPC. Finally, chondrocytes were shown to have the capacity to modulate pro-inflammatory macrophage activity by reducing MHC-II expression and TNF-α secretion. CONCLUSION:These data indicate the potential use of allogeneic chondrocytes in OA and cartilage defects. The lack of evident immunogenicity, despite exposure to a pro-inflammatory environment, coupled with the immunomodulatory ability indicates that these cells have the potential to evade the host immune system and suppress inflammation, thus potentially facilitating the resolution of OA induced inflammation and cartilage regeneration.


Lohan P,Treacy O,Lynch K,Barry F,Murphy M,Griffin MD,Ritter T,Ryan AE




Has Abstract


2016-03-01 00:00:00














  • Differential regulation of chondrocyte metabolism by oncostatin M and interleukin-6.

    abstract:OBJECTIVE:To determine the effects of interleukin (IL)-6 and oncostatin M (OSM) added separately or in combination with IL-1beta on human osteoarthritic (OA) chondrocytes in alginate beads. DESIGN:Human chondrocytes were isolated from OA cartilage and cultured in alginate beads for 12 days, in the absence or in the pr...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Sanchez C,Deberg MA,Burton S,Devel P,Reginster JY,Henrotin YE

    更新日期:2004-10-01 00:00:00

  • Brace treatment for osteoarthritis of the knee: a prospective randomized multi-centre trial.

    abstract:OBJECTIVE:To evaluate the effect of a brace intended to reduce load in patients with medial or lateral compartmental osteoarthritis (OA) and concurrent varus or valgus alignment, respectively. DESIGN:This multi-centre randomized controlled trial (performed 2001-2003) studies the additive effect of a brace intended to ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Brouwer RW,van Raaij TM,Verhaar JA,Coene LN,Bierma-Zeinstra SM

    更新日期:2006-08-01 00:00:00

  • Estrogen reduces mechanical injury-related cell death and proteoglycan degradation in mature articular cartilage independent of the presence of the superficial zone tissue.

    abstract:OBJECTIVE:To study the effect of 17β-estradiol (E2) and the superficial zone (SFZ) on cell death and proteoglycan degradation in articular cartilage after a single injurious compression in vitro. METHOD:Cartilage explants from the femoropatellar groove of 2 year old cows with or without the SFZ were cultured serum-fre...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Imgenberg J,Rolauffs B,Grodzinsky AJ,Schünke M,Kurz B

    更新日期:2013-11-01 00:00:00

  • Intra-articular injection of the selective cyclooxygenase-2 inhibitor meloxicam (Mobic) reduces experimental osteoarthritis and nociception in rats.

    abstract:OBJECTIVE:To study the effect of intra-articular injection of meloxicam (Mobic) on the development of osteoarthritis (OA) in rats and examine concomitant changes in nociceptive behavior and the expression of mitogen-activated protein kinases (MAPKs) in articular cartilage chondrocytes. METHODS:OA was induced in Wistar...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Wen ZH,Tang CC,Chang YC,Huang SY,Chen CH,Wu SC,Hsieh SP,Hsieh CS,Wang KY,Lin SY,Lee HL,Lee CH,Kuo HC,Chen WF,Jean YH

    更新日期:2013-12-01 00:00:00

  • Nondestructive assessment of sGAG content and distribution in normal and degraded rat articular cartilage via EPIC-microCT.

    abstract:OBJECTIVE:The objective of this study was to evaluate the feasibility of quantifying the Equilibrium Partitioning of an Ionic Contrast agent via Microcomputed Tomography (EPIC-microCT) to nondestructively assess sulfated glycosaminoglycan (sGAG) content and distribution in rat articular cartilage ex vivo, and in doing ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Xie L,Lin AS,Guldberg RE,Levenston ME

    更新日期:2010-01-01 00:00:00

  • Subchondral bone in osteoarthritis: association between MRI texture analysis and histomorphometry.

    abstract:OBJECTIVE:Magnetic resonance imaging (MRI) texture analysis is a method of analyzing subchondral bone alterations in osteoarthritis (OA). The objective of this study was to evaluate the association between MR texture analysis and ground-truth subchondral bone histomorphometry at the tibial plateau. DESIGN:The local re...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: MacKay JW,Murray PJ,Kasmai B,Johnson G,Donell ST,Toms AP

    更新日期:2017-05-01 00:00:00

  • Lack of beneficial effects of low-dose radiation therapy on hand osteoarthritis symptoms and inflammation: a randomised, blinded, sham-controlled trial.

    abstract:OBJECTIVES:Low-dose radiation therapy (LDRT) is widely used as treatment for osteoarthritis (OA) in some countries, while relatively unknown in others. Systematic literature review displayed a lack of high-level evidence for beneficial effects in clinical practice. The aim was to assess the efficacy of LDRT on symptoms...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Minten MJM,Leseman-Hoogenboom MM,Kloppenburg M,Kortekaas MC,Leer JW,Poortmans PMP,van den Hoogen FHJ,den Broeder AA,van den Ende CHM

    更新日期:2018-10-01 00:00:00

  • Prevalence of radiographic hip osteoarthritis is increased in high bone mass.

    abstract:OBJECTIVE:Epidemiological studies have shown an association between increased bone mineral density (BMD) and osteoarthritis (OA), but whether this represents cause or effect remains unclear. In this study, we used a novel approach to investigate this question, determining whether individuals with High Bone Mass (HBM) h...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Hardcastle SA,Dieppe P,Gregson CL,Hunter D,Thomas GE,Arden NK,Spector TD,Hart DJ,Laugharne MJ,Clague GA,Edwards MH,Dennison EM,Cooper C,Williams M,Davey Smith G,Tobias JH

    更新日期:2014-08-01 00:00:00

  • Intra-articular sodium hyaluronate in osteoarthritis of the knee: a multicenter, double-blind study.

    abstract::The efficacy and the safety of intra-articular injections of sodium hyaluronate were studied in patients with osteoarthritis of the knee in a randomized multicenter double-blind study. Two hundred and nine patients received five injections of either 25 mg hyaluronate/2.5 ml (verum, N = 102) or 0.25 mg hyaluronate/2.5 ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Puhl W,Bernau A,Greiling H,Köpcke W,Pförringer W,Steck KJ,Zacher J,Scharf HP

    更新日期:1993-10-01 00:00:00

  • Biological actions of curcumin on articular chondrocytes.

    abstract:OBJECTIVES:Curcumin (diferuloylmethane) is the principal biochemical component of the spice turmeric and has been shown to possess potent anti-catabolic, anti-inflammatory and antioxidant, properties. This article aims to provide a summary of the actions of curcumin on articular chondrocytes from the available literatu...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Henrotin Y,Clutterbuck AL,Allaway D,Lodwig EM,Harris P,Mathy-Hartert M,Shakibaei M,Mobasheri A

    更新日期:2010-02-01 00:00:00

  • Characterization of infiltrating T cells and Th1/Th2-type cytokines in the synovium of patients with osteoarthritis.

    abstract:OBJECTIVE:It has been suggested that osteoarthritis (OA) is induced by mechanical stress followed by cartilage destruction, and it is generally accepted that there is little involvement of an immune response in OA compared with that in rheumatoid arthritis (RA). We have previously found clonally expanded transcripts of...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Ishii H,Tanaka H,Katoh K,Nakamura H,Nagashima M,Yoshino S

    更新日期:2002-04-01 00:00:00

  • Gene therapy for meniscal injury: enhanced synthesis of proteoglycan and collagen by meniscal cells transduced with a TGFbeta(1)gene.

    abstract::Objective To determine whether meniscal cells can express a TGFbeta(1)transgene delivered by a retroviral vector, and respond to the gene product by increasing matrix synthesis. Methods Monolayer cultures of human and canine meniscal cells were infected with retroviruses carrying either a human TGFbeta(1)cDNA or marke...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Goto H,Shuler FD,Niyibizi C,Fu FH,Robbins PD,Evans CH

    更新日期:2000-07-01 00:00:00

  • T-cell mediated inflammatory pathway in osteoarthritis.

    abstract::Osteoarthritis (OA) is not caused by a simple consequence of aging and cartilage degradation. Based on the conventional paradigm, OA has been considered a degenerative joint disorder. However, the dominant clinical symptom has been characterized by a non-infectious chronic inflammatory condition with infiltration of i...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Nakamura H,Yoshino S,Kato T,Tsuruha J,Nishioka K

    更新日期:1999-07-01 00:00:00

  • Role of thigh muscle cross-sectional area and strength in progression of knee cartilage degeneration over 48 months - data from the Osteoarthritis Initiative.

    abstract:OBJECTIVE:To determine in a 48-month longitudinal study the association of thigh muscle cross-sectional area (CSA) and strength on progression of morphologic knee cartilage degeneration using 3T magnetic resonance imaging (MRI). DESIGN:Seventy Osteoarthritis Initiative (OAI) subjects aged 50-60 years, with no radiogra...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Goldman LH,Tang K,Facchetti L,Heilmeier U,Joseph GB,Nevitt MC,McCulloch CE,Souza RB,Link TM

    更新日期:2016-12-01 00:00:00

  • Anterior Cruciate Ligament OsteoArthritis Score (ACLOAS): Longitudinal MRI-based whole joint assessment of anterior cruciate ligament injury.

    abstract:OBJECTIVE:To develop a whole joint scoring system, the Anterior Cruciate Ligament OsteoArthritis Score (ACLOAS), for magnetic resonance imaging (MRI)-based assessment of acute anterior cruciate ligament (ACL) injury and follow-up of structural sequelae, and to assess its reliability. DESIGN:Baseline and follow-up 1.5 ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Roemer FW,Frobell R,Lohmander LS,Niu J,Guermazi A

    更新日期:2014-05-01 00:00:00

  • Recommendations for an update of the 2010 European regulatory guideline on clinical investigation of medicinal products used in the treatment of osteoarthritis and reflections about related clinically relevant outcomes: expert consensus statement.

    abstract:OBJECTIVE:The European Society on Clinical and Economic aspects of Osteoporosis and Osteoarthritis (ESCEO) organised a working group to evaluate the need for updating the current European guideline on clinical investigation of drugs used in the treatment of osteoarthritis (OA). DESIGN:Areas of potential attention were...

    journal_title:Osteoarthritis and cartilage

    pub_type: 共识发展会议,杂志文章,评审


    authors: Reginster JY,Reiter-Niesert S,Bruyère O,Berenbaum F,Brandi ML,Branco J,Devogelaer JP,Herrero-Beaumont G,Kanis J,Maggi S,Maheu E,Richette P,Rizzoli R,Cooper C

    更新日期:2015-12-01 00:00:00

  • Distinct expression pattern of early- and late-response genes in normal and osteoarthritic human synovial membranes.

    abstract::The significance of activating proteins (AP-1), c-fos, c-jun and jun B relative to the AP-1 responsive metallothionein, collagenase and stromelysin gene expression in the pathophysiology of osteoarthritis (OA) was investigated. The 'early' c-fos, c-jun and jun-B mRNAs were ubiquitously expressed in normal and OA human...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Zafarullah M,Martel-Pelletier J,Cloutier JM,Gedamu L,Pelletier JP

    更新日期:1993-07-01 00:00:00

  • Intermittent cyclic loading of cartilage explants modulates fibronectin metabolism.

    abstract:OBJECTIVE:The aim of this study was to evaluate systematically the effect of tissue load, its amplitude, time of intermittence and duration of loading on the biosynthesis and release of fibronectin by intermittently loaded mature bovine articular cartilage explants. METHODS:Cyclic compressive pressure was introduced u...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Steinmeyer J,Ackermann B,Raiss RX

    更新日期:1997-09-01 00:00:00

  • Comparison of mechanical debridement and radiofrequency energy for chondroplasty in an in vivo equine model of partial thickness cartilage injury.

    abstract:OBJECTIVE:The purpose of this study was to develop a long-term model of cartilage injury that could be used to compare the effects of radiofrequency energy (RFE) and mechanical debridement as a treatment. METHODS:Partial thickness fibrillation of patellar cartilage was created in 16 mature ponies. Three months after t...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Edwards RB 3rd,Lu Y,Uthamanthil RK,Bogdanske JJ,Muir P,Athanasiou KA,Markel MD

    更新日期:2007-02-01 00:00:00

  • NEL-like molecule-1-modified bone marrow mesenchymal stem cells/poly lactic-co-glycolic acid composite improves repair of large osteochondral defects in mandibular condyle.

    abstract:OBJECTIVE:Articular cartilage of the mandibular condyle has limited ability to regenerate itself after injury. This study was to investigate whether osteochondral defects in mandibular condyle could be repaired by NELL-1(NEL-like molecule-1)-modified autogenous bone marrow mesenchymal stem cells (BMMSCs) and poly lacti...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Zhu S,Zhang B,Man C,Ma Y,Hu J

    更新日期:2011-06-01 00:00:00

  • Site specific changes in gene expression and cartilage metabolism during early experimental osteoarthritis.

    abstract:OBJECTIVES:To characterize the molecular events underlying cartilage injury in the early phase of mono-iodoacetate-induced osteoarthritis (OA) in rats. METHODS:Experimental osteoarthritis was induced by intra-articular injection of 0.03mg mono-iodoacetate (MIA) in Wistar rats. Animals were killed 2, 5, 10, 15 and 20 d...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Dumond H,Presle N,Pottie P,Pacquelet S,Terlain B,Netter P,Gepstein A,Livne E,Jouzeau JY

    更新日期:2004-04-01 00:00:00

  • The validity of in vivo ultrasonographic grading of osteoarthritic femoral condylar cartilage: a comparison with in vitro ultrasonographic and histologic gradings.

    abstract:OBJECTIVES:To establish an ultrasonographic (US) grading for semiquantitative evaluation of the femoral condylar cartilage of knee osteoarthritis (OA), in vivo, and compare the in vivo US grading with the in vitro US and histologic gradings. DESIGN:Ninety-five patients going to receive total knee arthroplasty because ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Lee CL,Huang MH,Chai CY,Chen CH,Su JY,Tien YC

    更新日期:2008-03-01 00:00:00

  • Hypo-osmotic stress induces calcium-dependent actin reorganization in articular chondrocytes.

    abstract:OBJECTIVE:The aim of this study was to investigate the effects of hypo-osmotically induced calcium (Ca(2+)) transients on the organization of the actin cytoskeleton in articular chondrocytes. The secondary hypothesis tested was that actin restructuring following hypo-osmotic stress is mediated by gelsolin. METHODS:Iso...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Erickson GR,Northrup DL,Guilak F

    更新日期:2003-03-01 00:00:00

  • The volume and morphology of chondrocytes within non-degenerate and degenerate human articular cartilage.

    abstract:OBJECTIVE:Cartilage swelling is an early event in osteoarthritis (OA). However, the response of chondrocytes to increased tissue hydration is unknown. This work studied the volume and morphology of living in situ human chondrocytes as a function of cartilage degeneration. METHODS:The tibial plateaus from knee joints o...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Bush PG,Hall AC

    更新日期:2003-04-01 00:00:00

  • Changes in mechanics and composition of human talar cartilage anlagen during fetal development.

    abstract:OBJECTIVE:Fetal cartilage anlage provides a framework for endochondral ossification and organization into articular cartilage. We previously reported differences between mechanical properties of talar cartilage anlagen and adult articular cartilage. However, the underlying development-associated changes remain to be es...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Mahmoodian R,Leasure J,Philip P,Pleshko N,Capaldi F,Siegler S

    更新日期:2011-10-01 00:00:00

  • 2D and 3D MOCART scoring systems assessed by 9.4 T high-field MRI correlate with elementary and complex histological scoring systems in a translational model of osteochondral repair.

    abstract:OBJECTIVE:To compare the 2D and 3D MOCART system obtained with 9.4 T high-field magnetic resonance imaging (MRI) for the ex vivo analysis of osteochondral repair in a translational model and to correlate the data with semiquantitative histological analysis. METHODS:Osteochondral samples representing all levels of repa...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Goebel L,Zurakowski D,Müller A,Pape D,Cucchiarini M,Madry H

    更新日期:2014-10-01 00:00:00

  • Dicam promotes proliferation and maturation of chondrocyte through Indian hedgehog signaling in primary cilia.

    abstract:OBJECTIVES:Primary cilium is required for mechano-biological signal transduction in chondrocytes, and its interaction with extracellular matrix is critical for cartilage homeostasis. However, the role of cilia-associated proteins that affect the function of cilia remains to be elucidated. Here, we show that Dicam has a...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Han S,Park HR,Lee EJ,Jang JA,Han MS,Kim GW,Jeong JH,Choi JY,Beier F,Jung YK

    更新日期:2018-07-01 00:00:00

  • Surgical reconstruction of ruptured anterior cruciate ligament prolongs trauma-induced increase of inflammatory cytokines in synovial fluid: an exploratory analysis in the KANON trial.

    abstract:OBJECTIVE:Prospectively monitor how treatment of acutely ruptured anterior cruciate ligament (ACL) affects biomarkers of inflammation and proteolytic degradation over 5 years. DESIGN:We studied 119 subjects with acute ACL injury from the randomized controlled knee anterior cruciate ligament, non-surgical versus surgic...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Larsson S,Struglics A,Lohmander LS,Frobell R

    更新日期:2017-09-01 00:00:00

  • Sustained efficacy of a single intra-articular dose of FX006 in a rat model of repeated localized knee arthritis.

    abstract:OBJECTIVE:To evaluate the efficacy of a single intra-articular (IA) dose of FX006, an extended-release formulation of triamcinolone acetonide (TCA) in poly(lactic-co-glycolic acid) (PLGA) microspheres, on the sequelae of repeated episodes of synovitis. DESIGN:Three flares of localized synovitis in the right knee of ra...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Kumar A,Bendele AM,Blanks RC,Bodick N

    更新日期:2015-01-01 00:00:00

  • Osteophyte development--molecular characterization of differentiation stages.

    abstract:OBJECTIVE:Osteophytes are non-neoplastic osteo-cartilaginous protrusions growing at the margins of osteoarthritic joints. They can not only be considered as in situ repair tissue, but also represent an excellent in vivo model for induced cartilage repair processes. Our focus was to identify different steps of osteophyt...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Gelse K,Söder S,Eger W,Diemtar T,Aigner T

    更新日期:2003-02-01 00:00:00