Culture expanded primary chondrocytes have potent immunomodulatory properties and do not induce an allogeneic immune response.


OBJECTIVE:Allogeneic cell therapies, such as mesenchymal stromal cells (MSC), which have potent regenerative and anti-inflammatory potential are being investigated as a therapy for osteoarthritis (OA) and cartilage injury. Here we describe another potential source of regenerative and anti-inflammatory allogeneic cells, culture expanded primary chondrocytes (CEPC). In direct comparison to allogeneic MSC, we extensively assess the immunological interactions of CEPC in an allogeneic setting. METHODS:Chondrocytes were isolated from rat articular cartilage and cultured in normoxic or hypoxic conditions. In vitro co-culture assays with allogeneic lymphocytes and macrophages were used to assess the immunomodulatory capacities of the chondrocytes, followed by immune response analysis by flow cytometry, ELISA and qPCR. RESULTS:CEPC showed reduced induction of proliferation, activation and cytotoxic granzyme B expression in allogeneic T cells. Importantly, exposure to pro-inflammatory cytokines did not increase CEPC immunogenicity despite increases in MHC-I. Furthermore, CEPC had a potent ability to suppress allogeneic T cell proliferation, which was dependent on nitric oxide production. This suppression was contact independent in hypoxia cultured CEPC. Finally, chondrocytes were shown to have the capacity to modulate pro-inflammatory macrophage activity by reducing MHC-II expression and TNF-α secretion. CONCLUSION:These data indicate the potential use of allogeneic chondrocytes in OA and cartilage defects. The lack of evident immunogenicity, despite exposure to a pro-inflammatory environment, coupled with the immunomodulatory ability indicates that these cells have the potential to evade the host immune system and suppress inflammation, thus potentially facilitating the resolution of OA induced inflammation and cartilage regeneration.


Lohan P,Treacy O,Lynch K,Barry F,Murphy M,Griffin MD,Ritter T,Ryan AE




Has Abstract


2016-03-01 00:00:00














  • Human osteoarthritic synovium impacts chondrogenic differentiation of mesenchymal stem cells via macrophage polarisation state.

    abstract:OBJECTIVE:Mesenchymal stem cells (MSCs) are a promising cell type for the repair of damaged cartilage in osteoarthritis (OA). However, OA synovial fluid and factors secreted by synovium impede chondrogenic differentiation of MSCs, and the mechanism responsible for this effect remains unclear. In this study, we sought t...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Fahy N,de Vries-van Melle ML,Lehmann J,Wei W,Grotenhuis N,Farrell E,van der Kraan PM,Murphy JM,Bastiaansen-Jenniskens YM,van Osch GJ

    更新日期:2014-08-01 00:00:00

  • A pilot study of the reproducibility and validity of measuring knee subchondral bone density in the tibia.

    abstract:OBJECTIVE:To describe the reproducibility and validity of six different measurement techniques for knee subchondral bone mineral density (sBMD). METHODS:A consecutive sample of 50 male and female participants from a population-based longitudinal study had sBMD assessed using dual energy X-ray absorptiometry scans. Ant...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Dore D,Ding C,Jones G

    更新日期:2008-12-01 00:00:00

  • The surgical destabilization of the medial meniscus (DMM) model of osteoarthritis in the 129/SvEv mouse.

    abstract:OBJECTIVE:To evaluate anterior cruciate ligament transection (ACLT) and destabilization of the medial meniscus (DMM) surgical instability models of osteoarthritis (OA) in the 129/SvEv mouse knee joint. DESIGN:Micro-surgical techniques were used to perform ACLT or DMM under direct visualization. Histological scoring wa...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Glasson SS,Blanchet TJ,Morris EA

    更新日期:2007-09-01 00:00:00

  • Dynamic compression counteracts IL-1 beta-induced release of nitric oxide and PGE2 by superficial zone chondrocytes cultured in agarose constructs.

    abstract:OBJECTIVE:To examine the effect of IL-1 beta-induced *NO and PGE(2)release by stimulated superficial and deep chondrocyte/agarose constructs subjected to mechanical compression. DESIGN:Chondrocyte sub-populations were seeded separately in agarose constructs and cultured unstrained, within a 24-well tissue culture plat...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Chowdhury TT,Bader DL,Lee DA

    更新日期:2003-09-01 00:00:00

  • Natural history of cartilage damage and osteoarthritis progression on magnetic resonance imaging in a population-based cohort with knee pain.

    abstract:OBJECTIVES:To determine the natural history of cartilage damage and of osteoarthritis (OA) progression using magnetic resonance imaging (MRI); to evaluate whether OA progression varies by stage of disease. METHODS:A population-based cohort with knee pain was assessed clinically, with X-ray (Kellgren-Lawrence [KL] grad...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Cibere J,Sayre EC,Guermazi A,Nicolaou S,Kopec JA,Esdaile JM,Thorne A,Singer J,Wong H

    更新日期:2011-06-01 00:00:00

  • Systematic review of non-surgical therapies for osteoarthritis of the hand: an update.

    abstract:OBJECTIVE:To update our earlier systematic review which evaluated all published randomized controlled trials (RCTs) evaluating pharmacological and non-pharmacological therapies in patients with hand osteoarthritis (OA). Surgical therapies were not evaluated. METHOD:RCTs published between August 2004 and February 2008 ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Mahendira D,Towheed TE

    更新日期:2009-10-01 00:00:00

  • Effect of hyaluronic acid (MW 500-730 kDa) on proteoglycan and nitric oxide production in human osteoarthritic chondrocyte cultures exposed to hydrostatic pressure.

    abstract:OBJECTIVE:This study investigated the in vitro effects of hyaluronic acid (HA) of molecular weight (MW) 500-730 kDa on human articular chondrocytes cultivated for 48 h in the presence of interleukin-1beta (IL-1beta) with and without hydrostatic cyclical pressure. DESIGN:The effects of 10 and 100 microg/ml HA with and ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Fioravanti A,Cantarini L,Chellini F,Manca D,Paccagnini E,Marcolongo R,Collodel G

    更新日期:2005-08-01 00:00:00

  • The efficacy, safety and carry-over effect of diacerein in the treatment of painful knee osteoarthritis: a randomised, double-blind, NSAID-controlled study.

    abstract:OBJECTIVE:To evaluate the efficacy, safety and carry-over effect of diacerein, in comparison to piroxicam, in the treatment of Thai patients with symptomatic knee osteoarthritis (OA). DESIGN:This was a double-blind, randomised, piroxicam-controlled, parallel-group study. A 7-day non-steroidal anti-inflammatory drug wa...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Louthrenoo W,Nilganuwong S,Aksaranugraha S,Asavatanabodee P,Saengnipanthkul S,Thai Study Group.

    更新日期:2007-06-01 00:00:00

  • The effects of radial shock waves on gene transfer in rabbit chondrocytes in vitro.

    abstract:OBJECTIVE:The purpose of this study was to develop a new technique of gene transfer utilizing radial shock waves. The effects of radial shock waves on gene transfer in rabbit chondrocytes were examined by varying the parameters of exposure conditions in vitro. METHODS:Chondrocytes were obtained from New Zealand white ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Murata R,Nakagawa K,Ohtori S,Ochiai N,Arai M,Saisu T,Sasho T,Takahashi K,Moriya H

    更新日期:2007-11-01 00:00:00

  • Comparison of synovial fluid cartilage marker concentrations and chondral damage assessed arthroscopically in acute knee injury.

    abstract:OBJECTIVE:To examine the correlation between synovial fluid cartilage markers and degree of cartilage damage determined by arthroscopic evaluation in subjects with acute knee injury. DESIGN:Chondral damage was quantified using a validated arthroscopic scoring system in 20 subjects with effusive acute knee injuries of ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Bello AE,Garrett WE Jr,Wang H,Lohnes J,DeLong E,Caterson B,Kraus VB

    更新日期:1997-11-01 00:00:00

  • Psychometric properties of the French translation of the reduced KOOS and HOOS (KOOS-PS and HOOS-PS).

    abstract:OBJECTIVE:To evaluate the psychometric properties of the French KOOS physical function (KOOS-PS) and HOOS physical function (HOOS-PS), specifically its feasibility, reliability, construct validity, and responsiveness. METHODS:Consecutive outpatients consulting for primary knee or hip osteoarthritis (OA) in a rheumatol...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Ornetti P,Perruccio AV,Roos EM,Lohmander LS,Davis AM,Maillefert JF

    更新日期:2009-12-01 00:00:00

  • The longitudinal relationship between hand, hip and knee osteoarthritis and cardiovascular events: a population-based cohort study.

    abstract:OBJECTIVE:In this population-based cohort study, we examined the association between the presence of symptomatic osteoarthritis (OA) and risk for cardiovascular (CV) events. METHOD:A cohort aged ≥55 years recruited from 1996 to 98 was followed through provincial health administrative data to 2014. Demographics, joint ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Kendzerska T,Jüni P,King LK,Croxford R,Stanaitis I,Hawker GA

    更新日期:2017-11-01 00:00:00

  • Preoperative progressive explosive-type resistance training is feasible and effective in patients with hip osteoarthritis scheduled for total hip arthroplasty--a randomized controlled trial.

    abstract:OBJECTIVE:To investigate the efficacy and feasibility of progressive explosive-type resistance training (RT) in patients with osteoarthritis (OA) of the hip scheduled for total hip arthroplasty (THA). METHOD:Randomized controlled trial (1:1) in patients diagnosed with hip OA and scheduled for THA. The intervention gro...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Hermann A,Holsgaard-Larsen A,Zerahn B,Mejdahl S,Overgaard S

    更新日期:2016-01-01 00:00:00

  • Avocado soybean unsaponifiables (ASU) suppress TNF-alpha, IL-1beta, COX-2, iNOS gene expression, and prostaglandin E2 and nitric oxide production in articular chondrocytes and monocyte/macrophages.

    abstract:OBJECTIVE:To evaluate the effects of avocado soybean unsaponifiables (ASU) on proinflammatory mediators in chondrocytes and monocyte/macrophage-like cells. DESIGN:To determine the dose response of ASU, chondrocytes (5 x 10(5) cells/well) were incubated at 5% CO(2), 37 degrees C for 72 h with (1) control media alone or...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Au RY,Al-Talib TK,Au AY,Phan PV,Frondoza CG

    更新日期:2007-11-01 00:00:00

  • Measurement properties of performance-based outcome measures to assess physical function in young and middle-aged people known to be at high risk of hip and/or knee osteoarthritis: a systematic review.

    abstract:OBJECTIVES:To systematically appraise the evidence on measurement properties of performance-based outcome measures to assess physical function in young and middle-aged people known to be at high risk of hip and/or knee osteoarthritis (OA). METHODS:Electronic searches were performed in MEDLINE, CINAHL, Scopus and SPORT...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Kroman SL,Roos EM,Bennell KL,Hinman RS,Dobson F

    更新日期:2014-01-01 00:00:00

  • Bone mineral distribution of the proximal tibia in gonarthrosis assessed in vivo by photon absorption.

    abstract::Regional bone mineral density (BMD, g/cm2) of the proximal tibia including the subchondral plate was measured in vivo by dual photon absorptiometry (DPA). Twenty-one women and six men with gonarthrosis (GA) were examined. Twenty-four healthy women served as controls for the women. The precision error of the BMD measur...

    journal_title:Osteoarthritis and cartilage

    pub_type: 临床试验,杂志文章


    authors: Madsen OR,Schaadt O,Bliddal H,Egsmose C,Sylvest J

    更新日期:1994-06-01 00:00:00

  • Relationship between cartilage glycosaminoglycan content (assessed with dGEMRIC) and OA risk factors in meniscectomized patients.

    abstract:OBJECTIVE:To study the relationship between cartilage integrity, assessed with [delayed Gadolinium-Enhanced Magnetic Resonance Imaging of Cartilage (dGEMRIC)] and epidemiologic risk factors for knee osteoarthritis (OA) in meniscectomized patients. METHODS:Body mass index (BMI) was calculated in 45 patients (16 women),...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Ericsson YB,Tjörnstrand J,Tiderius CJ,Dahlberg LE

    更新日期:2009-05-01 00:00:00

  • Effectiveness of autologous chondrocyte implantation in cartilage repair of the knee: a systematic review of controlled trials.

    abstract:OBJECTIVE:The relative differences in effectiveness of subchondral stimulation, osteochondral grafts, and autologous chondrocyte implantation (ACI) are still unclear. It is the objective of this study to systematically review the literature on ACI compared to other treatments by clinical outcome and the quality of the ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Vavken P,Samartzis D

    更新日期:2010-06-01 00:00:00

  • Depth-dependent compressive properties of normal aged human femoral head articular cartilage: relationship to fixed charge density.

    abstract:OBJECTIVES:Determine the depth-varying confined and osmotic compression moduli of normal human articular cartilage from the femoral head, and test whether these moduli are dependent on fixed charge density. METHODS AND RESULTS:Using an automated instrument to allow epifluorescence microscopy analysis during confined c...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Chen SS,Falcovitz YH,Schneiderman R,Maroudas A,Sah RL

    更新日期:2001-08-01 00:00:00

  • Weight-bearing asymmetry and vertical activity differences in a rat model of post-traumatic knee osteoarthritis.

    abstract:OBJECTIVE:This study used a rat model of post-traumatic knee osteoarthritis (OA) created by anterior cruciate ligament transection with partial medial meniscectomy (ACLT + pMMx). In this model, mild to moderate structural changes that are typical of knee OA have been observed within 2 and 8 weeks post-surgery. We aimed...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Hamilton CB,Pest MA,Pitelka V,Ratneswaran A,Beier F,Chesworth BM

    更新日期:2015-07-01 00:00:00

  • Fluorophore-assisted carbohydrate electrophoresis (FACE) of glycosaminoglycans.

    abstract:OBJECTIVE:Quantitation and analyses of the fine structure of glycosaminoglycans are increasingly important for understanding many biological processes, including those most critical for understanding skeletal biology. We have developed a novel procedure, fluorophore-assisted carbohydrate electrophoresis (FACE), for det...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Calabro A,Midura R,Wang A,West L,Plaas A,Hascall VC

    更新日期:2001-01-01 00:00:00

  • Flow cytometric analysis of the human articular chondrocyte phenotype in vitro.

    abstract:OBJECTIVE:To develop flow cytometry for the study of human articular cartilage cell phenotype and to validate the method on chondrocytes cultured in different in-vitro systems. METHODS:Chondrocyte phenotype was modulated by culturing the cells under different in-vitro conditions: i.e. in monolayer and in suspension cu...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Wang L,Verbruggen G,Almqvist KF,Elewaut D,Broddelez C,Veys EM

    更新日期:2001-01-01 00:00:00

  • Single cell sorting identifies progenitor cell population from full thickness bovine articular cartilage.

    abstract:OBJECTIVE:To date, no approved clinical intervention successfully prevents the progressive degradation of injured articular cartilage that leads to osteoarthritis (OA). Stem/progenitor cell populations within tissues of diarthrodial joint have shown their therapeutic potential in treating OA. However, this potential ha...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Yu Y,Zheng H,Buckwalter JA,Martin JA

    更新日期:2014-09-01 00:00:00

  • Mechanical symptoms as an indication for knee arthroscopy in patients with degenerative meniscus tear: a prospective cohort study.

    abstract:OBJECTIVE:According to prevailing consensus, patients with mechanical symptoms are those considered to most likely benefit from arthroscopic surgery. The aim of this study was to determine the value of using patients' pre-operative self-reports of mechanical symptoms as a justification surgery in patients with degenera...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Sihvonen R,Englund M,Turkiewicz A,Järvinen TL

    更新日期:2016-08-01 00:00:00

  • Brace treatment for osteoarthritis of the knee: a prospective randomized multi-centre trial.

    abstract:OBJECTIVE:To evaluate the effect of a brace intended to reduce load in patients with medial or lateral compartmental osteoarthritis (OA) and concurrent varus or valgus alignment, respectively. DESIGN:This multi-centre randomized controlled trial (performed 2001-2003) studies the additive effect of a brace intended to ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Brouwer RW,van Raaij TM,Verhaar JA,Coene LN,Bierma-Zeinstra SM

    更新日期:2006-08-01 00:00:00

  • Ankle motion influences the external knee adduction moment and may predict who will respond to lateral wedge insoles?: an ancillary analysis from the SILK trial.

    abstract:OBJECTIVE:Lateral wedge insoles are a potential simple treatment for medial knee osteoarthritis (OA) patients by reducing the external knee adduction moment (EKAM). However in some patients, an increase in their EKAM is seen. Understanding the role of the ankle joint complex in the response to lateral wedge insoles is ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Chapman GJ,Parkes MJ,Forsythe L,Felson DT,Jones RK

    更新日期:2015-08-01 00:00:00

  • Aging does not change the compressive stiffness of mandibular condylar cartilage in horses.

    abstract:OBJECTIVE:Aging can cause an increase in the stiffness of hyaline cartilage as a consequence of increased protein crosslinks. By induction of crosslinking, a reduction in the diffusion of solutions into the hyaline cartilage has been observed. However, there is a lack of knowledge about the effects of aging on the biop...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Mirahmadi F,Koolstra JH,Fazaeli S,Lobbezoo F,van Lenthe GH,Snabel J,Stoop R,Arbabi V,Weinans H,Everts V

    更新日期:2018-12-01 00:00:00

  • Risk factors for osteoarthritis: genetics.

    abstract::Although the multifactorial nature of osteoarthritis (OA) is well recognized, genetic factors have been found to be strong determinants of the disease. Evidence of a genetic influence of OA comes from a number of sources, including epidemiological studies of family history and family clustering, twin studies, and expl...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Spector TD,MacGregor AJ

    更新日期:2004-01-01 00:00:00

  • Hip muscle weakness in patients with symptomatic femoroacetabular impingement.

    abstract:OBJECTIVE:Femoroacetabular impingement (FAI) is a pathomechanical process, which may cause hip pain, disability and early development of hip osteoarthritis (OA) in young and active adults. Patients with FAI experience functional disability during dynamic weight-bearing activities, which could originate from weakness of...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Casartelli NC,Maffiuletti NA,Item-Glatthorn JF,Staehli S,Bizzini M,Impellizzeri FM,Leunig M

    更新日期:2011-07-01 00:00:00

  • Association between smoking and risk of knee osteoarthritis: a systematic review and meta-analysis.

    abstract:OBJECTIVE:To investigate the association between smoking and the risk for knee osteoarthritis (OA). DESIGN:Cohort, case-control, and cross-sectional studies were obtained from the Medline, Embase, and Web of Science databases. Estimates were calculated using a random-effects model. Subgroup analyses and meta-regressio...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,meta分析,评审


    authors: Kong L,Wang L,Meng F,Cao J,Shen Y

    更新日期:2017-06-01 00:00:00