Abstract:
:To date, only bar/pat gene derived from Streptomyces has been used to generate the commercial PPT-resistant crops currently available in the market. The limited source of bar/pat gene is probably what has caused the decrease in PPT-tolerance, which has become the main concern of those involved in field management programs. Although glutamine synthetase (GS) is the target enzyme of PPT, little study has been reported about engineering PPT-resistant plants with GS gene. Then, the plant-optimized GS gene from Oryza sativa (OsGS1S) was chemically synthesized in the present study by PTDS to identify a GS gene for developing PPT-tolerant plants. However, OsGS1S cannot be directly used for developing PPT-tolerant plants because of its poor PPT-resistance. Thus, we performed DNA shuffling on OsGS1S, and one highly PPT-resistant mutant with mutations in four amino acids (A63E, V193A, T293A and R295K) was isolated after three rounds of DNA shuffling and screening. Among the four amino acids substitutions, only R295K was identified as essential in altering PPT resistance. The R295K mutation has also never been previously reported as an important residue for PPT resistance. Furthermore, the mutant gene has been transformed into Saccharomyces cerevisiae and Arabidopsis to confirm its potential in developing PPT-resistant crops.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Tian YS,Xu J,Zhao W,Xing XJ,Fu XY,Peng RH,Yao QHdoi
10.1038/srep15495subject
Has Abstractpub_date
2015-10-23 00:00:00pages
15495issn
2045-2322pii
srep15495journal_volume
5pub_type
杂志文章abstract::Environmental cycles often influence the presence of animals, creating patterns at different temporal scales, which may mean that their effects overlap and/or interact. Interactions between diel and seasonal cycles have been reported to influence fish behaviour but little is known about such interactions in marine top...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38900-4
更新日期:2019-02-22 00:00:00
abstract::Pulmonary fibrosis is a serious lung disorder that can lead to respiratory failure. Here we show that transgenic mice expressing active renin from the liver (RenTgMK) developed progressive pulmonary fibrosis leading to impaired pulmonary function. Histological analyses revealed a marked increase in extracellular matri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15561
更新日期:2015-10-23 00:00:00
abstract::Interleukin 17 (IL-17) stimulates the osteogenic differentiation of progenitor cells in vitro through a synergy with bone morphogenetic protein (BMP)-2. This study investigates whether the diverse responses mediated by IL-17 in vivo also lead to enhanced BMP-2-induced bone formation. Since IL-17 is known to induce ost...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25564-9
更新日期:2018-05-08 00:00:00
abstract::In this study, a noninvasive quantitative measure was used to identify short and long term post-concussion syndrome (PCS) both from each other and from healthy control populations. We used Electrovestibulography (EVestG) for detecting neurophysiological PCS consequent to a mild traumatic brain injury (mTBI) in both sh...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15487-2
更新日期:2017-11-27 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-26716-7
更新日期:2018-06-18 00:00:00
abstract::Based on the first-principles evolutionary materials design, we report a stable boron Kagome lattice composed of triangles in triangles on a two-dimensional sheet. The Kagome lattice can be synthesized on a silver substrate, with selecting Mg atoms as guest atoms. While the isolated Kagome lattice is slightly twisted ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07518-9
更新日期:2017-08-04 00:00:00
abstract::Molecular crystals demonstrate drastically different behavior in solid and liquid state, mainly due to their difference in structural frameworks. Therefore, designing of unique structured molecular compound which can work at both these interfaces has been a challenge. Here, we present remarkable 'molecular' property b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14460
更新日期:2015-09-28 00:00:00
abstract::Regional abnormalities in brain structure and function, as well as disrupted connectivity, have been found repeatedly in adolescents with conduct disorder (CD). Yet, the large-scale brain topology associated with CD is not well characterized, and little is known about the systematic neural mechanisms of CD. We employe...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/srep37053
更新日期:2016-11-14 00:00:00
abstract::In humans, oxytocin (OT) enhances prosocial behaviour. However, it is still unclear how the prosocial effects of OT are modulated by emotional features and/or individuals' characteristics. In a placebo-controlled design, we tested 20 healthy male volunteers to investigate these behavioural and neurophysiological modul...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00384
更新日期:2012-01-01 00:00:00
abstract::In this study, the effect of post-deposition tetrafluoromethane (CF4) plasma treatment on the physical and electrical characteristics of an In2TiO5 based electrolyte-insulator-semiconductor (EIS) sensor was investigated. Post-deposition CF4 plasma treatment typically improved the crystalline structure and repaired dan...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39012-9
更新日期:2019-02-28 00:00:00
abstract::Antimicrobial resistance seriously threatened human health. Combination therapy is generally an effective strategy to fight resistance, while some data on its effects are conflicting. To explore the reasons, the fractional inhibitory concentration indexes (FICIs) of three designed combinations against methicillin-resi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25714-z
更新日期:2018-05-08 00:00:00
abstract::Carbon-based stationary phases for chromatographic separation have been commercially available since the 1980s. Porous graphitic carbon liquid chromatography columns are known to be highly resistant to aggressive mobile phases and extreme pH values of solvents and eluents, an important advantage compared to convention...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33075-w
更新日期:2018-10-03 00:00:00
abstract::Mutations at positions 70 and/or 91 in the core protein of genotype-1b, hepatitis C virus (HCV) are associated with hepatocellular carcinoma (HCC) risk in Asian patients. To evaluate this in a US population, the relationship between the percentage of 70 and/or 91 mutant HCV quasispecies in baseline serum samples of ch...
journal_title:Scientific reports
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1038/srep27025
更新日期:2016-06-01 00:00:00
abstract::A better understanding of the seroprevalence and specificity of influenza HA stem-directed broadly neutralizing antibodies (bNAbs) in the human population could significantly inform influenza vaccine design efforts. Here, we utilized probes comprising headless, HA stabilized stem (SS) to determine the prevalence, bind...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26538-7
更新日期:2018-06-05 00:00:00
abstract::Although large populations feel fatigue, the standardized medicinal therapy is currently absent. In this study, we determined whether 5-aminolevulinic acid (5-ALA) supplementation alleviates the feeling of fatigue in healthy subjects who feel chronic physical tiredness. Males and females between ages of 20 and 64 who ...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-020-72763-4
更新日期:2020-09-29 00:00:00
abstract::Micro-tube experiment has been implemented to understand the mechanisms of governing microcosmic fluid percolation and is extensively used in both fields of micro electromechanical engineering and petroleum engineering. The measured pressure difference across the microtube is not equal to the actual pressure differenc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16870-9
更新日期:2017-11-29 00:00:00
abstract::The aim of this study was to test the hypothesis that duplication/hybridization of functional domains of naturally occurring pellicle peptides amplified the inhibitory effect of hydroxyapatite crystal growth, which is related to enamel remineralization and dental calculus formation. Histatin 3, statherin, their functi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21854-4
更新日期:2018-02-28 00:00:00
abstract::Free-living animals must make dietary choices in terms of chemical and physical properties, depending on their digestive physiology and availability of food resources. Here we comprehensively evaluated the dietary choices of proboscis monkeys (Nasalis larvatus) consuming young leaves. We analysed the data for leaf tou...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42774
更新日期:2017-02-17 00:00:00
abstract::Invisibility cloak capable of hiding an object can be achieved by properly manipulating electromagnetic field. Such a remarkable ability has been shown in transformation and ray optics. Alternatively, it may be realistic to create a spatial cloak by means of confining electromagnetic field in three-dimensional arrayed...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28527
更新日期:2016-06-22 00:00:00
abstract::Human epididymis protein 4 (HE4) has been used as a biomarker of endometrial cancer (EC) in clinical practice. However, there remains a lack of systemic research on the critical values of HE4 for diagnosing different clinical stages and pathological types of EC. This study investigated the accuracy of human epididymis...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79960-1
更新日期:2021-01-12 00:00:00
abstract::Selective attention plays a prominent role in prioritizing information in working memory (WM), improving performance for attended representations. However, it remains unclear whether unattended WM representations suffer from information loss. Here we tested the hypothesis that within WM, selectively attending to an it...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-019-49577-0
更新日期:2019-09-18 00:00:00
abstract::While cerebrospinal fluid (CSF) biomarkers for Creutzfeldt-Jakob disease (CJD) are established and partly included in the diagnostic criteria, no blood biomarkers are available. Here, we assessed the utility of serum neurofilament light chain (NF-L) and tau protein in comparison to CSF markers (NF-L and phosphorylated...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38737
更新日期:2016-12-08 00:00:00
abstract::Organic nanofibers have found various applications in optoelectronic devices. In such devices, exciton diffusion is a major aspect concerning their efficiency. In the case of singlet excitons, Förster transfer is the mechanism responsible for this process. Temperature and morphology are factors known to influence exci...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32232-5
更新日期:2018-09-19 00:00:00
abstract::Knowledge of the genomic variation among different strains of a pathogenic microbial species can help in selecting optimal candidates for diagnostic assays and vaccine development. Pooled sequencing (Pool-seq) is a cost effective approach for population level genetic studies that require large numbers of samples such ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45771
更新日期:2017-03-31 00:00:00
abstract::Fc domain fusion can improve the therapeutic effects of relatively small biological molecules such as peptides, cytokines, and antibody fragments. Fc fusion proteins can also be used to enhance the cytotoxic effects of small bispecific antibodies (bsAbs). However, fragmentation of Fc fusion proteins, which mainly occu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35489-y
更新日期:2018-11-22 00:00:00
abstract::Emergence of multiple drug resistant strains of pathogenic bacteria calls for new initiatives to combat infectious diseases. Gold nanoparticles (AuNPs), because of their non-toxic nature and size/shape dependent optical properties, offer interesting possibility. Here we report the antibacterial efficacy of AuNPs of di...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09357-0
更新日期:2017-08-29 00:00:00
abstract::The high growth rate of Ulva seaweeds makes it a potential algal biomass resource. In particular, Ulva meridionalis grows up to fourfold a day. Here, we demonstrated strong carbon fixation by U. meridionalis using 13C stable isotope labelling and traced the 13C flux through sugar metabolites with isotope-ratio mass sp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77237-1
更新日期:2020-11-23 00:00:00
abstract::As antibody-based diagnosis and therapy grow at an increased pace, there is a need for methods which rapidly and accurately determine antibody-antigen interactions. Here, we report a method for the multiplex determination of antibody epitopes using bacterial cell-surface display. A protein-fragment library with 10(7) ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00706
更新日期:2012-01-01 00:00:00
abstract::Genome editing tools have revolutionized the generation of genetically modified animals including livestock. In particular, the domestic pig is a proven model of human physiology and an agriculturally important species. In this study, we utilized the CRISPR/Cas9 system to edit the NANOS2 gene in pig embryos to generat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40176
更新日期:2017-01-10 00:00:00
abstract::The discovery of Weyl semimetals (WSM) has brought forth the condensed matter realization of Weyl fermions, which were previously theorized as low energy excitations in high energy particle physics. Recently, transition metal mono-pnictides are under intense investigation for understanding properties of inversion-symm...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46062
更新日期:2017-04-10 00:00:00