Identification of a phosphinothricin-resistant mutant of rice glutamine synthetase using DNA shuffling.

Abstract:

:To date, only bar/pat gene derived from Streptomyces has been used to generate the commercial PPT-resistant crops currently available in the market. The limited source of bar/pat gene is probably what has caused the decrease in PPT-tolerance, which has become the main concern of those involved in field management programs. Although glutamine synthetase (GS) is the target enzyme of PPT, little study has been reported about engineering PPT-resistant plants with GS gene. Then, the plant-optimized GS gene from Oryza sativa (OsGS1S) was chemically synthesized in the present study by PTDS to identify a GS gene for developing PPT-tolerant plants. However, OsGS1S cannot be directly used for developing PPT-tolerant plants because of its poor PPT-resistance. Thus, we performed DNA shuffling on OsGS1S, and one highly PPT-resistant mutant with mutations in four amino acids (A63E, V193A, T293A and R295K) was isolated after three rounds of DNA shuffling and screening. Among the four amino acids substitutions, only R295K was identified as essential in altering PPT resistance. The R295K mutation has also never been previously reported as an important residue for PPT resistance. Furthermore, the mutant gene has been transformed into Saccharomyces cerevisiae and Arabidopsis to confirm its potential in developing PPT-resistant crops.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Tian YS,Xu J,Zhao W,Xing XJ,Fu XY,Peng RH,Yao QH

doi

10.1038/srep15495

subject

Has Abstract

pub_date

2015-10-23 00:00:00

pages

15495

issn

2045-2322

pii

srep15495

journal_volume

5

pub_type

杂志文章
  • Genotype-guided warfarin dosing may benefit patients with mechanical aortic valve replacements: randomized controlled study.

    abstract::This prospective, single-blind, randomized study was designed to evaluate the effect of genotype-based warfarin dosing compared with standard warfarin dosing in Korean patients with mechanical cardiac valves. Patients were assigned to either the genotype-based dosing group or the standard dosing group using stratified...

    journal_title:Scientific reports

    pub_type: 杂志文章,随机对照试验

    doi:10.1038/s41598-020-63985-7

    authors: Lee KE,Yee J,Lee GY,Chung JE,Seong JM,Chang BC,Gwak HS

    更新日期:2020-04-24 00:00:00

  • Response of Human Osteoblast to n-HA/PEEK--Quantitative Proteomic Study of Bio-effects of Nano-Hydroxyapatite Composite.

    abstract::Nano-sized hydroxyapatite (n-HA) is considered as a bio-active material, which is often mixed into bone implant material, polyetheretherketone (PEEK). To reveal the global protein expression modulations of osteoblast in response to direct contact with the PEEK composite containing high level (40%) nano-sized hydroxyap...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep22832

    authors: Zhao M,Li H,Liu X,Wei J,Ji J,Yang S,Hu Z,Wei S

    更新日期:2016-03-09 00:00:00

  • A combined method to quantify the retinal metabolic rate of oxygen using photoacoustic ophthalmoscopy and optical coherence tomography.

    abstract::Quantitatively determining physiological parameters at a microscopic level in the retina furthers the understanding of the molecular pathways of blinding diseases, such as diabetic retinopathy and glaucoma. An essential parameter, which has yet to be quantified noninvasively, is the retinal oxygen metabolic rate (rMRO...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep06525

    authors: Song W,Wei Q,Liu W,Liu T,Yi J,Sheibani N,Fawzi AA,Linsenmeier RA,Jiao S,Zhang HF

    更新日期:2014-10-06 00:00:00

  • Selectivity and ligand-based molecular modeling of an odorant-binding protein from the leaf beetle Ambrostoma quadriimpressum (Coleoptera: Chrysomelidae) in relation to habitat-related volatiles.

    abstract::In this study, the most abundant and antenna-specific odorant-binding protein (OBP) of the elm pest A. quadriimpressum, AquaOBP4, was expressed and purified. The selectivity of AquaOBP4 was investigated by screening against a panel of 40 habitat-relevant compounds. Based on the obtained results, a homologous model of ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-15538-8

    authors: Wang Y,Jin Y,Chen Q,Wen M,Zhao H,Duan H,Ren B

    更新日期:2017-11-13 00:00:00

  • Uncovering the sources of DNA found on the Turin Shroud.

    abstract::The Turin Shroud is traditionally considered to be the burial cloth in which the body of Jesus Christ was wrapped after his death approximately 2000 years ago. Here, we report the main findings from the analysis of genomic DNA extracted from dust particles vacuumed from parts of the body image and the lateral edge use...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep14484

    authors: Barcaccia G,Galla G,Achilli A,Olivieri A,Torroni A

    更新日期:2015-10-05 00:00:00

  • A new model isolates glioblastoma clonal interactions and reveals unexpected modes for regulating motility, proliferation, and drug resistance.

    abstract::Tumor clonal heterogeneity drives treatment resistance. But robust models are lacking that permit eavesdropping on the basic interaction network of tumor clones. We developed an in vitro, functional model of clonal cooperation using U87MG glioblastoma cells, which isolates fundamental clonal interactions. In this mode...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-53850-7

    authors: Davis JB,Krishna SS,Abi Jomaa R,Duong CT,Espina V,Liotta LA,Mueller C

    更新日期:2019-11-22 00:00:00

  • Randomizing bipartite networks: the case of the World Trade Web.

    abstract::Within the last fifteen years, network theory has been successfully applied both to natural sciences and to socioeconomic disciplines. In particular, bipartite networks have been recognized to provide a particularly insightful representation of many systems, ranging from mutualistic networks in ecology to trade networ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep10595

    authors: Saracco F,Di Clemente R,Gabrielli A,Squartini T

    更新日期:2015-06-01 00:00:00

  • The characteristic, antioxidative and multiple organ protective of acidic-extractable mycelium polysaccharides by Pleurotus eryngii var. tuoliensis on high-fat emulsion induced-hypertriglyceridemic mice.

    abstract::The antioxidant and multiple organ protection effects of acid- extracted mycelia polysaccharides (Ac-MPS) from Pleurotus eryngii var. tuoliensis on HFE-induced hypertriglyceridemic mice were investigated. The results showed that Ac-MPS have potential ability to relieve the hypertriglyceridemia and preventing oxidative...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-35706-8

    authors: Gao Z,Lai Q,Yang Q,Xu N,Liu W,Zhao F,Liu X,Zhang C,Zhang J,Jia L

    更新日期:2018-11-30 00:00:00

  • Ultrahigh-Q metallic nanocavity resonances with externally-amplified intracavity feedback.

    abstract::We propose a mechanism of ultrahigh-Q metallic nanocavity resonances that involves an efficient loss-compensation scheme favorable for room-temperature operation. We theoretically show that surface plasmon-polaritons excited on the entrance and exit interfaces of a metallic nanocavity array efficiently transfer extern...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep07124

    authors: Yoon JW,Song SH,Magnusson R

    更新日期:2014-11-20 00:00:00

  • Lithia/(Ir, Li2IrO3) nanocomposites for new cathode materials based on pure anionic redox reaction.

    abstract::Anionic redox reactions attributed to oxygen have attracted much attention as a new approach to overcoming the energy-density limits of cathode materials. Several oxides have been suggested as new cathode materials with high capacities based on anionic (oxygen) redox reactions. Although most still have a large portion...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-49806-6

    authors: Lee SY,Park YJ

    更新日期:2019-09-12 00:00:00

  • Novel method for immunofluorescence staining of mammalian eggs using non-contact alternating-current electric-field mixing of microdroplets.

    abstract::Recently, a new technique was developed for non-catalytically mixing microdroplets. In this method, an alternating-current (AC) electric field is used to promote the antigen-antibody reaction within the microdroplet. Previously, this technique has only been applied to histological examinations of flat structures, such...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep15371

    authors: Shirasawa H,Kumagai J,Sato E,Kabashima K,Kumazawa Y,Sato W,Miura H,Nakamura R,Nanjo H,Minamiya Y,Akagami Y,Terada Y

    更新日期:2015-10-19 00:00:00

  • Author Correction: Whole exome sequencing reveals rare variants linked to congenital pouch colon.

    abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-018-32119-5

    authors: Mathur P,Medicherla KM,Chaudhary S,Patel M,Bagali P,Suravajhala P

    更新日期:2018-09-11 00:00:00

  • Insecticidal and Genotoxic effects of some indigenous plant extracts in Culex quinquefasciatus Say Mosquitoes.

    abstract::Five different weed plants viz. Convulvulus arvensis, Chenopodium murale, Tribulus terrestris, Trianthema portulacastrum, and Achyranthes aspera were investigated for their entomocidal and genotoxic effects against Culex quinquefasciatus mosquitoes. High mortality was observed at 72 hours in a dose dependent manner. A...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-63815-w

    authors: Zulhussnain M,Zahoor MK,Rizvi H,Zahoor MA,Rasul A,Ahmad A,Majeed HN,Rasul A,Ranian K,Jabeen F

    更新日期:2020-04-22 00:00:00

  • Endothelial dysfunction in systemic lupus erythematosus - a case-control study and an updated meta-analysis and meta-regression.

    abstract::Endothelium-dependent flow-mediated dilation (ED-FMD), a biophysical marker of endothelial dysfunction, is apparently impaired in patients with systemic lupus erythematosus (SLE) but such observation is inconsistent. Here, we assessed and compared the brachial artery ED-FMD (baED-FMD) using ultrasonography between SLE...

    journal_title:Scientific reports

    pub_type: 杂志文章,meta分析

    doi:10.1038/s41598-017-07574-1

    authors: Mak A,Kow NY,Schwarz H,Gong L,Tay SH,Ling LH

    更新日期:2017-08-04 00:00:00

  • The effects of different enrofloxacin dosages on clinical efficacy and resistance development in chickens experimentally infected with Salmonella Typhimurium.

    abstract::To investigate the optimal dosage which can improve clinical efficacy and minimize resistance, pharmacokinetics/pharmacodynamics model of enrofloxacin was established. Effect of enrofloxacin treatments on clearance of Salmonella in experimentally infected chickens and simultaneously resistance selection in Salmonella ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-12294-7

    authors: Li J,Hao H,Cheng G,Wang X,Ahmed S,Shabbir MAB,Liu Z,Dai M,Yuan Z

    更新日期:2017-09-15 00:00:00

  • Arrhythmogenic effects of mutated L-type Ca 2+-channels on an optogenetically paced muscular pump in Caenorhabditis elegans.

    abstract::Cardiac arrhythmias are often associated with mutations in ion channels or other proteins. To enable drug development for distinct arrhythmias, model systems are required that allow implementing patient-specific mutations. We assessed a muscular pump in Caenorhabditis elegans. The pharynx utilizes homologues of most o...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep14427

    authors: Schüler C,Fischer E,Shaltiel L,Steuer Costa W,Gottschalk A

    更新日期:2015-09-24 00:00:00

  • Selective tracking of FFAR3-expressing neurons supports receptor coupling to N-type calcium channels in mouse sympathetic neurons.

    abstract::Activation of short-chain free fatty acid receptors 3 (FFAR3) has been suggested to promote sympathetic outflow in postganglionic sympathetic neurons or hamper it by a negative coupling to N-type calcium (CaV2.2) channels. Heterogeneity of FFAR3 expression in sympathetic neurons, however, renders single neurons studie...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-35690-z

    authors: Colina C,Puhl HL 3rd,Ikeda SR

    更新日期:2018-11-26 00:00:00

  • Corrigendum: Treponema pallidum (syphilis) antigen TpF1 induces angiogenesis through the activation of the IL-8 pathway.

    abstract::This corrects the article DOI: 10.1038/srep18785. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/srep46945

    authors: Pozzobon T,Facchinello N,Bossi F,Capitani N,Benagiano M,Di Benedetto G,Zennaro C,West N,Codolo G,Bernardini M,Baldari CT,D'Elios MM,Pellegrini L,Argenton F,de Bernard M

    更新日期:2018-02-16 00:00:00

  • Ultrasound Powered Implants: Design, Performance Considerations and Simulation Results.

    abstract::Ultrasounds (US) has been used in the past decades as a non-invasive imaging modality. Although employed extensively in clinical applications for soft tissue imaging, the acoustic beams can also be used for sensing and actuation for biological implants. In this paper we present a unified three dimensional (3D) computa...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-63097-2

    authors: Rosa BMG,Yang GZ

    更新日期:2020-04-16 00:00:00

  • Host mechanisms involved in cattle Escherichia coli O157 shedding: a fundamental understanding for reducing foodborne pathogen in food animal production.

    abstract::The host mechanisms involved in Escherichia coli O157 super-shedding in cattle is largely unknown. In this study, the comparison of transcriptomes of intestinal tissues between super-shedders (SS) and cattle negative for E. coli O157 (NS) was performed, aiming to identify genes that are potentially associated with sup...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-06737-4

    authors: Wang O,McAllister TA,Plastow G,Stanford K,Selinger B,Guan LL

    更新日期:2017-08-09 00:00:00

  • Neural basis of uncertain cue processing in trait anxiety.

    abstract::Individuals with high trait anxiety form a non-clinical group with a predisposition for an anxiety-related bias in emotional and cognitive processing that is considered by some to be a prerequisite for psychiatric disorders. Anxious individuals tend to experience more worry under uncertainty, and processing uncertain ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep21298

    authors: Zhang M,Ma C,Luo Y,Li J,Li Q,Liu Y,Ding C,Qiu J

    更新日期:2016-02-19 00:00:00

  • Increased Susceptibility to Plasmodium falciparum in Infants is associated with Low, not High, Placental Malaria Parasitemia.

    abstract::Risk of malaria in infants can be influenced by prenatal factors. In this study, the potential for placental parasitemia at delivery in predicting susceptibility of infants to Plasmodium falciparum (Pf) infections was evaluated. Seventy-two newborns of mothers who were placental malaria negative (PM-) and of mothers w...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-18574-6

    authors: Tassi Yunga S,Fouda GG,Sama G,Ngu JB,Leke RGF,Taylor DW

    更新日期:2018-01-09 00:00:00

  • Evaluation of Macaca radiata as a non-human primate model of Dengue virus infection.

    abstract::Dengue virus (DENV) causes a wide range of illnesses in humans, including dengue fever and dengue haemorrhagic fever. Current animal models of DENV infection are limited for understanding infectious diseases in humans. Bonnet monkeys (Macaca radiata), a type of Old World monkey, have been used to study experimental an...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21582-9

    authors: Kato F,Ishida Y,Kawakami A,Takasaki T,Saijo M,Miura T,Hishiki T

    更新日期:2018-02-21 00:00:00

  • All-optical broadband ultrasonography of single cells.

    abstract::Cell mechanics play a key role in several fundamental biological processes, such as migration, proliferation, differentiation and tissue morphogenesis. In addition, many diseased conditions of the cell are correlated with altered cell mechanics, as in the case of cancer progression. For this there is much interest in ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep08650

    authors: Dehoux T,Abi Ghanem M,Zouani OF,Rampnoux JM,Guillet Y,Dilhaire S,Durrieu MC,Audoin B

    更新日期:2015-03-03 00:00:00

  • Trios-promising in silico biomarkers for differentiating the effect of disease on the human microbiome network.

    abstract::Recent advances in the HMP (human microbiome project) research have revealed profound implications of the human microbiome to our health and diseases. We postulated that there should be distinctive features associated with healthy and/or diseased microbiome networks. Following Occam's razor principle, we further hypot...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-12959-3

    authors: Ma ZS,Ye D

    更新日期:2017-10-16 00:00:00

  • Wavelength-converted wave-guiding in dye-doped polymer nanofibers.

    abstract::Nanoscale wavelength-converted optical components are promising components for communication and optical information processing in integrated photonic system. In this work, we report a facile strategy for realizing continuously tunable wavelength-converted wave-guiding in dye-doped nanofibers. The nanofibers with diam...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep01674

    authors: Yu H,Li B

    更新日期:2013-01-01 00:00:00

  • The coelacanth rostral organ is a unique low-resolution electro-detector that facilitates the feeding strike.

    abstract::The cartilaginous and non-neopterygian bony fishes have an electric sense typically comprised of hundreds or thousands of sensory canals distributed in broad clusters over the head. This morphology facilitates neural encoding of local electric field intensity, orientation, and polarity, used for determining the positi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep08962

    authors: Berquist RM,Galinsky VL,Kajiura SM,Frank LR

    更新日期:2015-03-11 00:00:00

  • Amyloid precursor protein expression and processing are differentially regulated during cortical neuron differentiation.

    abstract::Amyloid precursor protein (APP) and its cleavage product amyloid β (Aβ) have been thoroughly studied in Alzheimer's disease. However, APP also appears to be important for neuronal development. Differentiation of induced pluripotent stem cells (iPSCs) towards cortical neurons enables in vitro mechanistic studies on hum...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29200

    authors: Bergström P,Agholme L,Nazir FH,Satir TM,Toombs J,Wellington H,Strandberg J,Bontell TO,Kvartsberg H,Holmström M,Boreström C,Simonsson S,Kunath T,Lindahl A,Blennow K,Hanse E,Portelius E,Wray S,Zetterberg H

    更新日期:2016-07-07 00:00:00

  • The effects of repetitive transcranial magnetic stimulation in an animal model of tinnitus.

    abstract::Tinnitus (phantom auditory perception associated with hearing loss) can seriously affect wellbeing. Its neural substrate is unknown however it has been linked with abnormal activity in auditory pathways. Though no cure currently exists, repetitive transcranial magnetic stimulation (rTMS) has been shown to reduce tinni...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep38234

    authors: Mulders WH,Vooys V,Makowiecki K,Tang AD,Rodger J

    更新日期:2016-12-01 00:00:00

  • Satellite radar observation of large surface collapses induced by the 2017 North Korea nuclear test.

    abstract::The artificial earthquake of mb 6.1 related to the North Korea's sixth nuclear test occured at Mt. Mantap, North Korea on September 3, 2017. It was reported that a large and complex surface deformation was caused by the event. The surface deformation was composed of expansion of explosions, collapse, compaction and la...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-74957-2

    authors: Baek WK,Jung HS,Kim TS

    更新日期:2020-10-20 00:00:00