Abstract：
:Broadly neutralizing antibodies developed from the IGHV1-69 germline gene are known to bind to the stem region of hemagglutinin in diverse influenza viruses but the sequence determinants for the antigen recognition, including neutralization potency and binding affinity, are not clearly understood. Such understanding could inform designs of synthetic antibody libraries targeting the stem epitope on hemagglutinin, leading to artificially designed antibodies that are functionally advantageous over antibodies from natural antibody repertoires. In this work, the sequence space of the complementarity determining regions of a broadly neutralizing antibody (F10) targeting the stem epitope on the hemagglutinin of a strain of H1N1 influenza virus was systematically explored; the elucidated antibody-hemagglutinin recognition principles were used to design a phage-displayed antibody library, which was then used to discover neutralizing antibodies against another strain of H1N1 virus. More than 1000 functional antibody candidates were selected from the antibody library and were shown to neutralize the corresponding strain of influenza virus with up to 7 folds higher potency comparing with the parent F10 antibody. The antibody library could be used to discover functionally effective antibodies against other H1N1 influenza viruses, supporting the notion that target-specific antibody libraries can be designed and constructed with systematic sequence-function information.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Tung CP,Chen IC,Yu CM,Peng HP,Jian JW,Ma SH,Lee YC,Jan JT,Yang ASdoi
10.1038/srep15053subject
Has Abstractpub_date
2015-10-12 00:00:00pages
15053issn
2045-2322pii
srep15053journal_volume
5pub_type
杂志文章abstract：:CRISPR/Cas9 is a valuable tool for both basic and applied research that has been widely applied to different plant species. Nonetheless, a systematical assessment of the efficiency of this method is not available for the allotetraploid Brassica napus-an important oilseed crop. In this study, we examined the mutation e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07871-9
更新日期：2017-08-08 00:00:00
abstract：:The increasing incidence of obesity accentuates the importance of identifying mechanisms and optimal therapeutic strategies for patients with heart failure (HF) in relation to obesity status. Here, we investigated the association between plasma level of apelin, an adipocyte-derived factor, and clinicopathological feat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16104
更新日期：2015-11-06 00:00:00
abstract：:Blood transfusion is fundamental in managing hematologic malignancies. We sought to evaluate the need and availability of blood products for patients with hematological malignancies at Uganda Cancer Institute. We prospectively studied the demand and supply of blood for patients with thrombocytopenia (platelet count ≤5...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59773-y
更新日期：2020-02-18 00:00:00
abstract：:Influenza virus remains an emerging virus and causes pandemics with high levels of fatality. After screening different plant extracts with potential anti-influenza activity, a water extract of Taxodium distichum stems (TDSWex) showed excellent activity against influenza viruses. The EC50 of TDSWex was 0.051 ± 0.024 mg...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36015
更新日期：2016-10-31 00:00:00
abstract：:Unambiguous identification of trace amounts of biochemical molecules in a complex background using terahertz spectroscopy is extremely challenging owing to the extremely small absorption cross sections of these molecules in the terahertz regime. Herein, we numerically propose a terahertz nonresonant nano-slits structu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60732-w
更新日期：2020-02-28 00:00:00
abstract：:Distinct regions of long-range genetic fixation in the human MHC region, known as conserved extended haplotypes (CEHs), possess unique genomic characteristics and are strongly associated with numerous diseases. While CEHs appear to be homogeneous by SNP analysis, the nature of fine variations within their genomic stru...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16972
更新日期：2015-11-23 00:00:00
abstract：:Rapid diagnostic tests (RDTs) have been widely deployed in low-resource settings. These tests are typically read by visual inspection, and accurate record keeping and data aggregation remains a substantial challenge. A successful malaria elimination campaign will require new strategies that maximize the sensitivity of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28645
更新日期：2016-06-27 00:00:00
abstract：:The anisotropic shape of DNA molecules allows them to form lyotropic liquid crystals (LCs) at high concentrations. This liquid crystalline arrangement is also found in vivo (e.g., in bacteriophage capsids, bacteria or human sperm nuclei). However, the role of DNA liquid crystalline organization in living organisms sti...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22863-z
更新日期：2018-03-14 00:00:00
abstract：:Tumor clonal heterogeneity drives treatment resistance. But robust models are lacking that permit eavesdropping on the basic interaction network of tumor clones. We developed an in vitro, functional model of clonal cooperation using U87MG glioblastoma cells, which isolates fundamental clonal interactions. In this mode...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53850-7
更新日期：2019-11-22 00:00:00
abstract：:The aminopeptidase gene from Mesorhizobium SEMIA3007 was cloned and overexpressed in Escherichia coli. The enzyme called MesoAmp exhibited optimum activity at pH 8.5 and 45 °C and was strongly activated by Co2+ and Mn2+. Under these reaction conditions, the enzyme displayed Km and kcat values of 0.2364 ± 0.018 mM and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10932-8
更新日期：2017-09-06 00:00:00
abstract：:Algae-bacteria interaction is one of the main factors underlying the formation of harmful algal blooms (HABs). The aim of this study was to develop a genome-wide high-throughput screening method to identify HAB-influenced specific interactive bacterial metabolites using a comprehensive collection of gene-disrupted E. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67322-w
更新日期：2020-06-30 00:00:00
abstract：:Melanoma patients with BRAF mutations respond to treatment with vemurafenib, thus creating a need for accurate testing of BRAF mutation status. We carried out a blinded study to evaluate various BRAF mutation testing methodologies in the clinical setting. Formalin-fixed, paraffin-embedded melanoma samples were macrodi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01659
更新日期：2013-01-01 00:00:00
abstract：:The nucleotides guanosine tetraphosphate and pentaphosphate (together known as (p)ppGpp or magic spot) are produced in plant plastids from GDP/GTP and ATP by RelA-SpoT homologue (RSH) enzymes. In the model plant Arabidopsis (p)ppGpp regulates chloroplast transcription and translation to affect growth, and is also impl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54207-w
更新日期：2019-11-27 00:00:00
abstract：:Low-dimensional semiconductor structurers formed on a substrate surface at pre-defined locations and with nano-precision placement is of vital interest. The potential of tailoring their electrical and optical properties will revolutionize the next generation of optoelectronic devices. Traditionally, highly aligned sel...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50633-y
更新日期：2019-10-02 00:00:00
abstract：:This paper presents the correlative imaging of collagen denaturation by nonlinear optical microscopy (NLO) and nanoscale infrared (IR) spectroscopy to obtain morphological and chemical information at different length scales. Such multiscale correlated measurements are applied to the investigation of ancient parchments...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26344
更新日期：2016-05-19 00:00:00
abstract：:The intravenous, intramuscular or intraperitoneal administration of water solubilized graphene nanoparticles for biomedical applications will result in their interaction with the hematological components and vasculature. Herein, we have investigated the effects of dextran functionalized graphene nanoplatelets (GNP-Dex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02584
更新日期：2013-01-01 00:00:00
abstract：:During chronic cerebral hypoperfusion (CCH), the cerebral blood flow gradually decreases, leading to cognitive impairments and neurodegenerative disorders, such as vascular dementia. The reduced oxygenation, energy supply induced metabolic changes, and insufficient neuroplasticity could be reflected in the synaptic pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72868-w
更新日期：2020-09-29 00:00:00
abstract：:The prediction of infarction volume after stroke onset depends on the shape of the growth dynamics of the infarction. To understand growth patterns that predict lesion volume changes, we studied currently available models described in literature and compared the models with Adaptive Neuro-Fuzzy Inference System [ANFIS...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08044-4
更新日期：2017-08-08 00:00:00
abstract：:On standard electrocardiogram (ECG) PQ interval is known to be moderately heart rate dependent, but no physiologic details of this dependency have been established. At the same time, PQ dynamics is a clear candidate for non-invasive assessment of atrial abnormalities including the risk of atrial fibrillation. We studi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59480-8
更新日期：2020-02-13 00:00:00
abstract：:Despite extensive efforts in studying radioactive aerosols, including the transmission of radionuclides in different chemical matrices throughout the body, the internal organ-specific radiation dose due to inhaled radioactive aerosols has largely relied on experimental deposition data and simplified human phantoms. Co...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54040-1
更新日期：2019-11-25 00:00:00
abstract：:Here, we describe a new strategy that allows the rapid and efficient engineering of mono and multispecific trivalent antibodies. By fusing single-domain antibodies from camelid heavy-chain-only immunoglobulins (VHHs) to the N-terminus of a human collagen XVIII trimerization domain (TIE(XVIII)) we produced monospecific...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28643
更新日期：2016-06-27 00:00:00
abstract：:Adult-onset vitelliform macular dystrophy (AVMD) is a common and benign macular degeneration which can be caused by BEST1 mutation. Here, we investigated the clinical characteristics associated with a newly identified BEST1 mutation, p.Ile38Ser and confirmed the associated physiological functional defects. The 51-year...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09629-9
更新日期：2017-08-22 00:00:00
abstract：:The metabolic balance of the most extensive bioma on the Earth is a controversial topic of the global-change research. High ultraviolet radiation (UVR) levels by the shoaling of upper mixed layers and increasing atmospheric dust deposition from arid regions may unpredictably alter the metabolic state of marine oligotr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35892
更新日期：2016-10-24 00:00:00
abstract：:The objective of this study was to examine the relationship between sleep duration and ischemic and hemorrhagic stroke in a community-based cohort. The current analysis included 95,023 Chinese participants who were free of stroke at the baseline survey (2006-2007). Cox proportional hazards models were used to calculat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33664
更新日期：2016-09-16 00:00:00
abstract：:Activation of cell cycle regulated transcription during the G1-to-S transition initiates S phase entry and cell cycle commitment. The molecular mechanisms involving G1/S transcriptional regulation are well established and have been shown to be evolutionary conserved from yeast to humans. Previous work has suggested th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47170-z
更新日期：2019-07-23 00:00:00
abstract：:With millions of new dental and orthopedic implants inserted annually, periprosthetic osteolysis becomes a major concern. In dentistry, peri-implantitis management includes cleaning using ultrasonic scaling. We examined whether ultrasonic scaling releases titanium particles and induces inflammation and osteolysis. Tit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39612
更新日期：2017-01-06 00:00:00
abstract：:The fatty acid (FA) profile has a considerable impact on the nutritional and technological quality of milk and dairy products. The molecular mechanism underlying the regulation of fat metabolism in bovine mammary gland have been not completely elucidated. We conducted genome-wide association studies (GWAS) across 65 m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17434-7
更新日期：2017-12-11 00:00:00
abstract：:For one-dimensional (1d) nonlinear atomic lattices, the models with on-site nonlinearities such as the Frenkel-Kontorova (FK) and ϕ4 lattices have normal energy transport while the models with inter-site nonlinearities such as the Fermi-Pasta-Ulam-β (FPU-β) lattice exhibit anomalous energy transport. The 1d Discrete N...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23719-2
更新日期：2018-03-28 00:00:00
abstract：:We demonstrate that the propagation path of a surface acoustic wave (SAW), excited with an interdigitated transducer (IDT), can be visualized using a thin liquid film dispensed onto a lithium niobate (LiNbO3) substrate. The practical advantages of this visualization method are its rapid and simple implementation, with...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21980
更新日期：2016-02-26 00:00:00
abstract：:Hallmarks of Retinitis Pigmentosa (RP), a family of genetic diseases, are a typical rod-cone-degeneration with initial night blindness and loss of peripheral vision, followed by decreased daylight sight and progressive visual acuity loss up to legal blindness. Great heterogeneity in nature and function of mutated gene...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06045-x
更新日期：2017-07-18 00:00:00
