Abstract:
:The conserved Hedgehog (HH) signals control animal development, adult stem cell maintenance and oncogenesis. In Drosophila, the HH co-receptor Patched (PTC) controls both HH gradient formation and signalling. PTC is post-translationally downregulated by HH, which promotes its endocytosis and destabilization, but the mechanisms of PTC trafficking and its importance in the control of PTC remain to be understood. PTC interacts with E3 Ubiquitin (UB)-ligases of the C2-WW-HECT family; two of them-SMURF and NEDD4-are known to regulate its levels. We demonstrate that mutation of the PTC PY motif, which mediates binding of C2-WW-HECT family members, inhibits its internalization but not its autonomous and non-autonomous signalling activities. In addition, we show that the two related UB-C2-WW-HECT ligases NEDD4 and SU(DX) regulate PTC trafficking and finely tune its accumulation through partially redundant but distinct functions. While both NEDD4 and SU(DX) promote PTC endocytosis, only SU(DX) is able to induce its lysosomal targeting and degradation. In conclusion, PTC trafficking and homeostasis are tightly regulated by a family of UB-ligases.
journal_name
Open Bioljournal_title
Open biologyauthors
Brigui A,Hofmann L,Argüelles C,Sanial M,Holmgren RA,Plessis Adoi
10.1098/rsob.150112subject
Has Abstractpub_date
2015-10-01 00:00:00issue
10issn
2046-2441pii
rsob.150112journal_volume
5pub_type
杂志文章相关文献
Open Biology文献大全abstract::Unlike most bacteria, mycobacteria rely on the multi-domain enzyme eukaryote-like fatty acid synthase I (FAS I) to make fatty acids de novo. These metabolites are precursors of the biosynthesis of most of the lipids present both in the complex mycobacteria cell wall and in the storage lipids inside the cell. In order ...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.160277
更新日期:2017-02-01 00:00:00
abstract::Synaptic vesicle exocytosis relies on the tethering of release ready vesicles close to voltage-gated Ca2+ channels and specific lipids at the future site of fusion. This enables rapid and efficient neurotransmitter secretion during presynaptic depolarization by an action potential. Extensive research has revealed that...
journal_title:Open biology
pub_type: 杂志文章,评审
doi:10.1098/rsob.170258
更新日期:2018-02-01 00:00:00
abstract::Heat shock protein 90 (Hsp90) is a promising cancer drug target as a molecular chaperone critical for stabilization and activation of several of the oncoproteins that drive cancer progression. Its actions depend upon its essential ATPase, an activity fortuitously inhibited with a very high degree of selectivity by nat...
journal_title:Open biology
pub_type: 杂志文章,评审
doi:10.1098/rsob.120138
更新日期:2012-12-12 00:00:00
abstract::Deubiquitylating enzymes (DUBs) reverse the ubiquitylation of target proteins, thereby regulating diverse cellular functions. In contrast to the plethora of research being conducted on the ability of DUBs to counter the degradation of cellular proteins or auto-ubiquitylated E3 ligases, very little is known about the m...
journal_title:Open biology
pub_type: 杂志文章,评审
doi:10.1098/rsob.170016
更新日期:2017-06-01 00:00:00
abstract::Skeletal vasculature plays a central role in the maintenance of microenvironments for osteogenesis and haematopoiesis. In addition to supplying oxygen and nutrients, vasculature provides a number of inductive factors termed as angiocrine signals. Blood vessels drive recruitment of osteoblast precursors and bone format...
journal_title:Open biology
pub_type: 杂志文章,评审
doi:10.1098/rsob.190144
更新日期:2019-10-31 00:00:00
abstract::Vitamin B₁₂-dependent enzymes function in core biochemical pathways in Mycobacterium tuberculosis, an obligate pathogen whose metabolism in vivo is poorly understood. Although M. tuberculosis can access vitamin B₁₂ in vitro, it is uncertain whether the organism is able to scavenge B₁₂ during host infection. This quest...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.120175
更新日期:2013-02-13 00:00:00
abstract::Current ageing theories are far from satisfactory because of the many determinants involved in ageing. The well-known rate-of-living theory assumes that the product (lifetime energy expenditure, LEE) of maximum lifespan (MLS) and mass-specific basal metabolic rate (msBMR) is approximately constant. Although this theor...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.170083
更新日期:2017-10-01 00:00:00
abstract::Cancer cells undergo many different alterations during their transformation, including genetic and epigenetic events. The controlled division of healthy cells can be impaired through the downregulation of tumour suppressor genes. Here, we provide an update of the mechanisms in which epigenetically altered coding and n...
journal_title:Open biology
pub_type: 杂志文章,评审
doi:10.1098/rsob.170152
更新日期:2017-09-01 00:00:00
abstract::Tn5 transposase is a bacterial enzyme that integrates a DNA fragment into genomic DNA, and is used as a tool for detecting nucleosome-free regions of genomic DNA in eukaryotes. However, in chromatin, the DNA targeting by Tn5 transposase has remained unclear. In the present study, we reconstituted well-positioned 601 d...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.190116
更新日期:2019-08-30 00:00:00
abstract::T-cell identity is established by the expression of a clonotypic T-cell receptor (TCR), generated by somatic rearrangement of TCRα and β genes. The properties of the TCR determine both the degree of self-reactivity and the repertoire of antigens that can be recognized. For CD8 T cells, the relationship between TCR ide...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.160293
更新日期:2016-11-01 00:00:00
abstract::In recent years pleiotropy, the phenomenon of one genetic locus influencing several traits, has become a widely researched field in human genetics. With the increasing availability of genome-wide association study summary statistics, as well as the establishment of deeply phenotyped sample collections, it is now possi...
journal_title:Open biology
pub_type: 杂志文章,评审
doi:10.1098/rsob.170125
更新日期:2017-11-01 00:00:00
abstract::Somatostatin (SS) and allatostatin-C (ASTC) are structurally and evolutionarily related neuropeptides that act as inhibitory regulators of physiological processes in mammals and insects, respectively. Here, we report the first molecular and functional characterization of SS/ASTC-type signalling in a deuterostome inver...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.200172
更新日期:2020-09-01 00:00:00
abstract::Parkinson's disease (PD) is characterized by selective loss of dopaminergic neurons in the pars compacta of the substantia nigra and accumulation of ubiquitinated proteins in aggregates called Lewy bodies. Several mutated genes have been found in familial PD patients, including SNCA (α-synuclein), PARK2 (parkin), PINK...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.160193
更新日期:2016-08-01 00:00:00
abstract::The range of biological outcomes generated by many signalling proteins in development and homeostasis is increased by their interactions with glycosaminoglycans, particularly heparan sulfate (HS). This interaction controls the localization and movement of these signalling proteins, but whether such control depends on ...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.150277
更新日期:2016-03-01 00:00:00
abstract::The innate immune response is the first line of defence against infection. Germ-line-encoded receptors recognize conserved molecular motifs from both exogenous and endogenous sources. Receptor activation results in the initiation of a pro-inflammatory immune response that enables the resolution of infection. Understan...
journal_title:Open biology
pub_type: 杂志文章,评审
doi:10.1098/rsob.120015
更新日期:2012-04-01 00:00:00
abstract::Innate immunity relies entirely upon germ-line encoded receptors, signalling components and effector molecules for the recognition and elimination of invading pathogens. The fruit fly Drosophila melanogaster with its powerful collection of genetic and genomic tools has been the model of choice to develop ideas about i...
journal_title:Open biology
pub_type: 杂志文章,评审
doi:10.1098/rsob.120075
更新日期:2012-05-01 00:00:00
abstract::Genetic material can be transmitted not only vertically from parent to offspring, but also laterally (horizontally) from one bacterial lineage to another. Lateral genetic transfer is non-uniform; biases in its nature or frequency construct communities of genetic exchange. These biases have been proposed to arise from ...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.120112
更新日期:2012-09-01 00:00:00
abstract::We here address the question of whether there is any correlation between subjective preference for simple configurations within a specific visual domain such as motion and strength of activity in visual areas in which that domain is emphasized. We prepared several distinctive patterns of dots in motion with various ch...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.120001
更新日期:2012-02-01 00:00:00
abstract::DNA methylation has been investigated for many years, but recent technologies have allowed for single-cell- and single-base-resolution DNA methylation datasets and more accurate assessment of DNA methylation dynamics at the key genomic regions that regulate gene expression in human early embryonic development. In this...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.180131
更新日期:2018-10-31 00:00:00
abstract::Hsp70 chaperones interact with substrate proteins in a coordinated fashion that is regulated by nucleotides and enhanced by assisting cochaperones. There are numerous homologues and isoforms of Hsp70 that participate in a wide variety of cellular functions. This diversity can facilitate adaption or specialization base...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.200089
更新日期:2020-08-01 00:00:00
abstract::Intercalation allows cells to exchange positions in a spatially oriented manner in an array of diverse processes, spanning convergent extension in embryonic gastrulation to the formation of tubular organs. However, given the co-occurrence of cell intercalation and changes in cell shape, it is sometimes difficult to as...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.200329
更新日期:2020-11-01 00:00:00
abstract::Wnts are a highly conserved family of secreted glycoproteins that play essential roles in the morphogenesis and body patterning during the development of metazoan species. In recent years, mounting evidence has revealed important functions of Wnt signalling in diverse aspects of neural development, including neuronal ...
journal_title:Open biology
pub_type: 杂志文章,评审
doi:10.1098/rsob.180116
更新日期:2018-10-03 00:00:00
abstract::Cancer is considered a group of diseases characterized by uncontrolled growth and spread of abnormal cells and is propelled by somatic mutations. Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3) family of enzymes are endogenous sources of somatic mutations found in multiple human cancers. Wh...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.200188
更新日期:2020-12-01 00:00:00
abstract::The conserved process of centriole duplication requires Plk4 kinase to recruit and promote interactions between Sas6 and Sas5/Ana2/STIL (respective nomenclature of worms/flies/humans). Plk4-mediated phosphorylation of Ana2/STIL in its conserved STAN motif has been shown to promote its interaction with Sas6. However, S...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.170247
更新日期:2017-12-01 00:00:00
abstract::The genus Caulobacter is found in a variety of habitats and is known for its ability to thrive in low-nutrient conditions. K31 is a novel Caulobacter isolate that has the ability to tolerate copper and chlorophenols, and can grow at 4 ° C with a doubling time of 40 h. K31 contains a 5.5 Mb chromosome that codes for mo...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.140128
更新日期:2014-10-01 00:00:00
abstract::Typical centrioles are made of microtubules organized in ninefold symmetry. Most animal somatic cells have two centrioles for normal cell division and function. These centrioles originate from the zygote, but because the oocyte does not provide any centrioles, it is surprising that the zygotes of many animals are thou...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.160334
更新日期:2017-03-01 00:00:00
abstract::Redox conditions change in events such as immune and platelet activation, and during viral infection, but the biochemical consequences are not well characterized. There is evidence that some disulfide bonds in membrane proteins are labile while others that are probably structurally important are not exposed at the pro...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.110010
更新日期:2011-11-01 00:00:00
abstract::The kinetochore is a complex structure whose function is absolutely essential. Unlike the centromere, the kinetochore at first appeared remarkably well conserved from yeast to humans, especially the microtubule-binding outer kinetochore. However, recent efforts towards biochemical reconstitution of diverse kinetochore...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.200284
更新日期:2020-11-01 00:00:00
abstract::ThyX is an essential thymidylate synthase that is mechanistically and structurally unrelated to the functionally analogous human enzyme, thus providing means for selective inhibition of bacterial growth. To identify novel compounds with anti-bacterial activity against the human pathogenic bacterium Helicobacter pylori...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.150015
更新日期:2015-06-01 00:00:00
abstract::Pseudomonas aeruginosa are noscomially acquired, opportunistic pathogens that pose a major threat to the health of burns patients and the immunocompromised. We sequenced the genomes of P. aeruginosa isolates RNS_PA1, RNS_PA46 and RNS_PAE05, which displayed resistance to almost all frontline antibiotics, including gent...
journal_title:Open biology
pub_type: 杂志文章
doi:10.1098/rsob.150175
更新日期:2016-03-01 00:00:00