Abstract：
:While is widely acknowledged that nonmuscle myosin II (NMMII) enables stress fibers (SFs) to generate traction forces against the extracellular matrix, little is known about how specific NMMII isoforms and functional domains contribute to SF mechanics. Here we combine biophotonic and genetic approaches to address these open questions. First, we suppress the NMMII isoforms MIIA and MIIB and apply femtosecond laser nanosurgery to ablate and investigate the viscoelastic retraction of individual SFs. SF retraction dynamics associated with MIIA and MIIB suppression qualitatively phenocopy our earlier measurements in the setting of Rho kinase (ROCK) and myosin light chain kinase (MLCK) inhibition, respectively. Furthermore, fluorescence imaging and photobleaching recovery reveal that MIIA and MIIB are enriched in and more stably localize to ROCK- and MLCK-controlled central and peripheral SFs, respectively. Additional domain-mapping studies surprisingly reveal that deletion of the head domain speeds SF retraction, which we ascribe to reduced drag from actomyosin crosslinking and frictional losses. We propose a model in which ROCK/MIIA and MLCK/MIIB functionally regulate common pools of SFs, with MIIA crosslinking and motor functions jointly contributing to SF retraction dynamics and cellular traction forces.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Chang CW,Kumar Sdoi
10.1038/srep13736subject
Has Abstractpub_date
2015-09-04 00:00:00pages
13736issn
2045-2322pii
srep13736journal_volume
5pub_type
杂志文章abstract：:Multiple Sclerosis (MS), is a chronic inflammatory autoimmune disorder of the central nervous system that leads to chronic demyelination with axonal damage and neuronal loss. Mesenchymal stem cells (MSCs) represent a promising therapeutic approach for MS. In the current study, we investigated the effects of MSCs deriv...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19703-5
更新日期：2018-01-29 00:00:00
abstract：:Recent findings suggest an association between obesity, loss of gut barrier function and changes in microbiota profiles. Our primary objective was to examine the effect of caloric restriction and subsequent weight reduction on gut permeability in obese women. The impact on inflammatory markers and fecal microbiota was...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12109-9
更新日期：2017-09-20 00:00:00
abstract：:Randomizing the layer thickness of superlattices (SL) can lead to localization of coherent phonons and thereby reduces the lattice thermal conductivity κ l . In this work, we propose strategies that can suppress incoherent phonon transport in the above random multilayer (RML) structure to further reduce κ l . Molecula...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08359-2
更新日期：2017-08-15 00:00:00
abstract：:Lateral flow strip tests are a cost-effective method for detecting specific proteins in biological samples, which can be performed in the field without specialized expertise. While most recognizable in the pregnancy tests, there are many other applications for lateral flow strip technology. The pest control industry h...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70200-0
更新日期：2020-08-07 00:00:00
abstract：:Insertion sequence (IS) 6110 is found at multiple sites in the Mycobacterium tuberculosis genome and displays a high degree of polymorphism with respect to copy number and insertion sites. Therefore, IS6110 is considered to be a useful molecular marker for diagnosis and strain typing of M. tuberculosis. Generally IS61...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12567
更新日期：2015-07-28 00:00:00
abstract：:The distribution of attention between competing processing demands can have dramatic real-world consequences, however little is known about how limited attentional resources are distributed during real-world behaviour. Here we employ mobile EEG to characterise the allocation of attention across multiple sensory-cognit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51996-y
更新日期：2019-11-01 00:00:00
abstract：:The entire recrystallisation sequence and associated crystallographic texture evolution of Mg-0.8Zn-0.2Ca (wt.%) alloy was tracked using a quasi-in-situ electron backscatter diffraction (EBSD) method. Characteristic "Rare Earth" (RE) texture was formed, originating mainly from double twins and twinning-related shear b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43415-z
更新日期：2019-05-09 00:00:00
abstract：:This observational study investigated the impact of hyponatremia resolution on the results of a comprehensive geriatric assessment (CGA) in 150 patients with age ≥70 years and serum sodium <130 mEq/L. The test battery including Barthel index of Activities of Daily Living (ADL) and various tests of neurocognitive funct...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49054-8
更新日期：2019-08-29 00:00:00
abstract：:High-resolution spectroscopy in the 1-10 μm region has never been fully tackled for the lack of widely-tunable and practical light sources. Indeed, all solutions proposed thus far suffer from at least one of three issues: they are feasible only in a narrow spectral range; the power available for spectroscopy is limite...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12891-6
更新日期：2017-10-06 00:00:00
abstract：:Rubber-filler composites are a key component in the manufacture of tyres. The filler provides mechanical reinforcement and additional wear resistance to the rubber, but it in turn introduces non-linear mechanical behaviour to the material which most likely arises from interactions between the filler particles, mediate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07389
更新日期：2014-12-09 00:00:00
abstract：:This study demonstrated the usefulness of radiomic features based on the Hessian index of differential topology for the prediction of prognosis prior to treatment in head-and-neck (HN) cancer patients. The Hessian index, which can indicate tumor heterogeneity with convex, concave, and other points (saddle points), was...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78338-7
更新日期：2020-12-04 00:00:00
abstract：:This paper presented a conformal smoothing theory, and smoothing capability evaluation was established on the proposed theory. According to pressure distribution model, processing parameters have been optimized and the CPP sample with a size of 340 × 340 mm was applied in conformal smoothing. The middle spatial freque...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59484-4
更新日期：2020-02-13 00:00:00
abstract：:Arctic and alpine environments present extreme, but different, challenges to survival. We therefore studied the ecological adaptation of the arctic-alpine fritillary Boloria napaea in northern Sweden and compared these results with the eastern Alps. Using mark-release-recapture, we analysed phenology, mobility, activi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40508-7
更新日期：2019-03-07 00:00:00
abstract：:Studies suggest that chromium deficiency is associated with elevated levels of fasting blood glucose, circulating insulin, cholesterol and triglycerides, and decreased proportion of lean body mass. However, data directly relating chromium levels to metabolic syndrome (MetS) risk are lacking. A total of 3,648 American ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15606
更新日期：2015-10-22 00:00:00
abstract：:Microbial secondary metabolites are valuable resources for novel drug discovery. In particular, actinomycetes expressed a range of antibiotics against a spectrum of bacteria. In genus level, strain Allosalinactinospora lopnorensis CA15-2(T) is the first new actinomycete isolated from the Lop Nor region, China. Antimic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20667
更新日期：2016-02-11 00:00:00
abstract：:Promoter CpG methylation is a fundamental regulatory process of gene expression. TET proteins are active CpG demethylases converting 5-methylcytosine to 5-hydroxymethylcytosine, with loss of 5 hmC as an epigenetic hallmark of cancers, indicating critical roles of TET proteins in epigenetic tumorigenesis. Through analy...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26591
更新日期：2016-05-26 00:00:00
abstract：:The aim of the study was to investigate the effect of 2nd-line pazopanib on the different CTCs subpopulations in SCLC patients and evaluate the clinical relevance of their changes. Different CTCs subpopulations were evaluated before pazopanib initiation (n = 56 patients), after one-cycle (n = 35) and on disease progre...
journal_title：Scientific reports
pub_type： 杂志文章,多中心研究
doi：10.1038/s41598-018-20502-1
更新日期：2018-02-02 00:00:00
abstract：:This study aimed to evaluate the effects of ketorolac, a commonly used non-steroidal anti-inflammatory drug (NSAID) as patient controlled intravenous infusion analgesia (PCIA) for the patients underwent radical cystectomy (RC) due to bladder cancer regarding post-operational indices of recovery. Total seventy patients...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/s41598-018-22677-z
更新日期：2018-03-15 00:00:00
abstract：:Emerging infectious pathogens that threaten blood transfusions are known to be present in blood samples from healthy/qualified donors. The objective of this study was to investigate the microbiome of blood from healthy donors from the Luzhou area in southwestern China. Potential pathogens and cytomegalovirus (CMV) inf...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72808-8
更新日期：2020-09-25 00:00:00
abstract：:DNA is organized and compacted into higher-order structures in order to fit within nuclei and to facilitate gene regulation. Mechanisms by which higher order chromatin structures are established and maintained are poorly understood. In C. elegans, nuclear-localized small RNAs engage the nuclear RNAi machinery to regul...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45052-y
更新日期：2019-06-21 00:00:00
abstract：:Despite the detailed characterization of the inflammatory and endothelial changes observed in Sickle Cell Disease (SCD), the hierarchical relationship between elements involved in the pathogenesis of this complex disease is yet to be described. Meta-analyses of gene expression studies from public repositories represen...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep17822
更新日期：2015-12-09 00:00:00
abstract：:Functional magnetic resonance imaging (fMRI) based on the blood oxygen level dependent (BOLD) contrast has gained a prominent position in neuroscience for imaging neuronal activity and studying effective brain connectivity under working state and functional connectivity at resting state. However, the fundamental quest...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02864-0
更新日期：2017-06-02 00:00:00
abstract：:The discovery of novel ligand chemotypes allows to explore uncharted regions in chemical space, thereby potentially improving synthetic accessibility, potency, and the drug-likeness of molecules. Here, we demonstrate the scaffold-hopping ability of the new Weighted Holistic Atom Localization and Entity Shape (WHALES) ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34677-0
更新日期：2018-11-07 00:00:00
abstract：:The aim of this study was to investigate left ventricular (LV) global myocardial strain and LV involvement characteristics in patients with arrhythmogenic right ventricular dysplasia/cardiomyopathy (ARVD/C) and to evaluate their predictive value of adverse cardiac events. Sixty consecutive ARVD/C patients with a defin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50535-z
更新日期：2019-10-02 00:00:00
abstract：:Healthy neonates exhibit a well-functioning haemostatic system despite peculiarities regarding composition of clotting factors and inhibitors as well as impaired platelet aggregation. Thrombocytopenia and severe bleeding events are feared in sick infants. Recombinant factor VIIa (rFVIIa) is a haemostatic agent used as...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44199-y
更新日期：2019-05-29 00:00:00
abstract：:Telomerase is a ribonucleoprotein enzyme, which maintains genome integrity in eukaryotes and ensures continuous cellular proliferation. Telomerase holoenzyme from the thermotolerant yeast Hansenula polymorpha, in addition to the catalytic subunit (TERT) and telomerase RNA (TER), contains accessory proteins Est1 and Es...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68107-x
更新日期：2020-07-06 00:00:00
abstract：:Telomerase is expressed in adult mouse, but not in most human, tissues and mouse telomeres are much longer than those in humans. This interspecies difference of telomere homeostasis poses a challenge in modeling human diseases using laboratory mice. Using chromatinized bacterial artificial chromosome reporters, we dis...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46160-5
更新日期：2019-07-04 00:00:00
abstract：:The initiation of polymorphic ventricular tachycardia in long QT syndrome type 2 (LQT2) has been associated with a characteristic ECG pattern of short-long RR intervals. We hypothesize that this characteristic pattern increases APD dispersion in LQT2, thereby promoting arrhythmia. We investigated APD dispersion and it...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51230-9
更新日期：2019-10-16 00:00:00
abstract：:Regulatory T cells (Tregs) expand in peripheral lymphoid organs and can produce immunosuppressive cytokines to support tumor growth. IL-10 abrogation efficiently induces Treg formation but dampens tumoral neuropilin-1 (Nrp-1) Treg signaling, which simultaneously augments Th1 and Th17 immunity. These effects are associ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24249
更新日期：2016-04-14 00:00:00
abstract：:Gyroviruses (GyVs) are small, single-stranded, circular DNA viruses in the genus Gyrovirus, which consists of the chicken anemia virus (CAV) prototype and nine other viral species. These different GyV species have been reported in chickens, humans, mice, and companion animals. To date, CAV has been identified in the f...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49955-8
更新日期：2019-09-16 00:00:00
