Abstract:
:Based on evidence that human diploid fibroblasts (HDF) from the Werner syndrome (WS) of premature aging might overexpress an inhibitor of DNA synthesis (IDS), we prepared a eukaryotic cDNA expression library from WS mRNA and tested it for IDS activity in a transient assay. Two of six WS cDNA pools tested gave IDS activity, then on plus/minus screening revealed several differentially expressed cDNA clones. By slot blot and Northern analysis, one cDNA clone was found to be overexpressed in WS and normal senescent HDF, but not in quiescent normal HDF, indicating that it is senescence-specific. Further studies are needed to clarify: a) whether this cDNA truly acts as an IDS; b) if so, whether it acts alone or in concert with other cDNAs; and c) whether it is involved in the degenerative and malignant sequelae of WS and normal aging.
journal_name
Exp Gerontoljournal_title
Experimental gerontologyauthors
Goldstein S,Murano S,Benes H,Moerman EJ,Jones RA,Thweatt R,Shmookler Reis RJ,Howard BHdoi
10.1016/0531-5565(89)90052-1subject
Has Abstractpub_date
1989-01-01 00:00:00pages
461-8issue
5-6eissn
0531-5565issn
1873-6815journal_volume
24pub_type
杂志文章abstract::Post-translational oxidative protein modifications which are more marked during aging and/or high-calorie (HC) diets affect protein function and metabolism. Protein function and metabolism are different according to the type of muscle proteins. Oxidative muscle protein modifications may thus be associated with age-rel...
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journal_title:Experimental gerontology
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2016.10.013
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journal_title:Experimental gerontology
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(94)00053-0
更新日期:1995-03-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2012.07.010
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/s0531-5565(00)00091-7
更新日期:2000-05-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/s0531-5565(03)00111-6
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2003.10.011
更新日期:2004-02-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/s0531-5565(02)00239-5
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(83)90040-2
更新日期:1983-01-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2008.10.007
更新日期:2009-03-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2016.04.022
更新日期:2016-08-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2010.01.003
更新日期:2010-08-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(92)90034-w
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journal_title:Experimental gerontology
pub_type: 杂志文章
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
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pub_type: 杂志文章,多中心研究
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journal_title:Experimental gerontology
pub_type: 杂志文章
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更新日期:2003-10-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 历史文章,杂志文章
doi:10.1016/j.exger.2012.02.004
更新日期:2013-01-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2006.04.008
更新日期:2006-06-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2009.05.004
更新日期:2009-08-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2017.08.003
更新日期:2017-10-15 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2006.01.013
更新日期:2006-04-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2010.05.004
更新日期:2010-09-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2005.07.012
更新日期:2005-10-01 00:00:00