Abstract:
:It has been more than thirty years since the in vitro brainstem-spinal cord preparation was first presented as a method to study automatic breathing behaviors in the neonatal rat. This straightforward preparation has led to an incredible burst of information about the location and coordination of several spontaneously active microcircuits that form the ventrolateral respiratory network of the brainstem. Despite these advances, our knowledge of the mechanisms that regulate central breathing behaviors is still incomplete. Investigations into the nature of spontaneous breathing rhythmicity have almost exclusively focused on mammals, and there is a need for comparative experimental models to evaluate several unresolved issues from a different perspective. With this in mind, we sought to develop a new avian in vitro model with the long term goal to better understand questions associated with the ontogeny of respiratory rhythm generation, neuroplasticity, and whether multiple, independent oscillators drive the major phases of breathing. The fact that birds develop in ovo provides unparalleled access to central neuronal networks throughout the prenatal period - from embryo to hatchling - that are free from confounding interactions with mother. Previous studies using in vitro avian models have been strictly limited to the early embryonic period. Consequently, the details and even the presence of brainstem derived breathing-related rhythmogenesis in birds have never been described. In the present study, we used the altricial zebra finch (Taeniopygia guttata) and show robust spontaneous motor outflow through cranial motor nerve IX, which is first detectable on embryonic day four and continues through prenatal and early postnatal development without interruption. We also show that brainstem oscillations change dramatically over the course of prenatal development, sometimes within hours, which suggests rapid maturational modifications in growth and connectivity. We propose that this experimental preparation will be useful for a variety of studies aimed at testing the biophysical and synaptic properties of neurons that participate in the unique spatiotemporal patterns of avian breathing behaviors, especially in the context of early development.
journal_name
Respir Physiol Neurobioljournal_title
Respiratory physiology & neurobiologyauthors
Vincen-Brown MA,Whitesitt KC,Quick FG,Pilarski JQdoi
10.1016/j.resp.2015.08.007subject
Has Abstractpub_date
2016-04-01 00:00:00pages
62-70eissn
1569-9048issn
1878-1519pii
S1569-9048(15)30036-7journal_volume
224pub_type
杂志文章abstract::This study tested the hypothesis that, by increasing the volume available for tidal expansion (inspiratory capacity, IC), bi-level positive airway pressure (BiPAP™) would lead to greater beneficial effects on dyspnea and exercise intolerance in comorbid heart failure (HF)-chronic obstructive pulmonary disease (COPD) t...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2019.04.008
更新日期:2019-08-01 00:00:00
abstract::In response to the increase in oxygen tension at birth, the resistance pulmonary arteries dilate, while the ductus arteriosus constricts. Although modulated by the endothelium, these opposite responses are intrinsic to the vascular smooth muscle. While still controversial, it seems likely that during normoxia the prod...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,评审
doi:10.1016/s1569-9048(02)00054-x
更新日期:2002-08-22 00:00:00
abstract::We developed and tested a semi-automated algorithm to generate large data sets of ventilatory information (amplitude, premotor drive and timing) across a range of motor behaviors. Adult spontaneously breathing, anesthetized mice (n = 27) underwent measurements of transdiaphragmatic pressure (Pdi) during eupnea, hypoxi...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2015.05.009
更新日期:2015-08-15 00:00:00
abstract::Within the moderate exercise intensity domain, the speed of oxygen uptake (V(O(2))) kinetics at the transition to a higher metabolic rate is thought to be limited by an inertia of the oxidative machinery. Nitric oxide (NO)-induced inhibition of O(2) consumption within the electron transport chain may contribute to thi...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/s1569-9048(02)00068-x
更新日期:2002-08-30 00:00:00
abstract::Although dyspnoea has been shown to attenuate pain, whether different forms of dyspnoea exert a similar inhibitory effect on pain has never been tested. We examined the effects of two different forms of dyspnoea, i.e., "air hunger" sensation (AIR HUNGER) and "work/effort" sensation (WORK/EFFORT), on pain induced by a ...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2011.05.013
更新日期:2011-08-15 00:00:00
abstract::Airway responses to intravenous (i.v.) and inhaled (i.h.) delivery of methacholine (MCh) in BALB/c and C57BL/6 mouse strains have been compared with and without ovalbumin (OVA)-induced airway inflammation. Bronchial reactivity to MCh was assessed in anaesthetised and tracheostomised animals by using an animal ventilat...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2008.12.005
更新日期:2009-02-28 00:00:00
abstract::Breathing movements require the coordinated recruitment of cranial and spinal motoneurons innervating muscles of the upper airway and ribcage. A significant part of respiratory motoneuron development and maturation occurs prenatally to support the generation of fetal breathing movements in utero and sustained breathin...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,评审
doi:10.1016/j.resp.2005.03.017
更新日期:2005-11-15 00:00:00
abstract::The adult elephant is unique among mammals in that the pleural membranes are thickened and the pleural cavity is obliterated by connective tissue. It has been suggested that this peculiar anatomy developed because the animal can snorkel at depth, and this behavior subjects the microvessels in the parietal pleura to a ...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/s1569-9048(03)00199-x
更新日期:2003-11-14 00:00:00
abstract::The trigeminal and olfactory systems interact during sensory processing of odor. Here, we investigate odor-evoked modulations of brainstem respiratory networks in a decerebrated perfused brainstem preparation of rat with intact olfactory bulbs. Intranasal application of non-trigeminal odors (rose) did not evoke respir...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2016.07.008
更新日期:2016-11-01 00:00:00
abstract:AIMS:Quantitatively measure and validate analysis of neural respiratory drive (NRD) using a commercial polysomnography system in children during sleep. METHOD:Surface electromyogram of the diaphragm (sEMGdi) recorded from primary snoring children were analysed. A subset was re-analysed to assess intra- and inter-inves...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2017.02.004
更新日期:2017-05-01 00:00:00
abstract::In chronic heart failure (HF), the alveolar-capillary membrane undergoes a remodeling process that negatively affects gas exchange. In case of alveolar-capillary gas diffusion impairment, arterial desaturation (SaO2) is rarely observed in HF patients. At play are 3 factors: overall pulmonary diffusing capacity (assess...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2016.06.004
更新日期:2016-11-01 00:00:00
abstract::DLCO measured in hypoxia must be corrected due to the higher affinity (increase in coefficient θ) of CO with Hb. We propose an adjustment accounting for individual changes in the equation relating DLCO to subcomponents Dm (membrane diffusive capacity) and Vc (lung capillary volume): 1/DLCO=1/Dm+1/θVc. We adjusted the ...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2016.08.009
更新日期:2017-04-01 00:00:00
abstract::Eucalyptol is a compound that has demonstrated antioxidant, anti-inflammatory and bronchodilator effects, but there are no investigations about the effects of this constituent on the respiratory system mechanics in relation to acute lung injury caused by short-term cigarette smoke (CS) exposure. In view of the above, ...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2019.04.007
更新日期:2019-08-01 00:00:00
abstract::The retrotrapezoid nucleus (RTN), located at the ventral surface of the medulla oblongata, contains glutamatergic Phox2b-expressing interneurons that have central respiratory chemoreceptor properties. RTN also operates as a relay for hypothalamic pathways that regulate breathing, one of which probably originates from ...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2010.12.003
更新日期:2011-02-15 00:00:00
abstract::There are many reports of olfactory impairment in patients with Parkinson's disease (PD) and the impairment can be observed before the appearance of typical PD symptom. Accordingly, olfactory screening tests may predict disease onset and indicates a need for early treatment before classic signs of the disease. Olfacti...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2008.01.003
更新日期:2008-04-30 00:00:00
abstract::Increased transdiaphragmatic pressure, reduced muscle blood flow, and increased duty cycle have all been associated with a reduction in the center frequency (CFdi) of the diaphragm's electrical activity (EAdi). However, the specific influence of diaphragm activation on CFdi is unknown. We evaluated whether increased d...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2004.11.006
更新日期:2005-03-01 00:00:00
abstract::Recently, we reported that dyspnea on exertion is strongly associated with an increased oxygen cost of breathing in otherwise healthy obese women; the mechanism of dyspnea on exertion in obese men is unknown. Obese men underwent measurements of body composition, fat distribution, pulmonary function, steady state and m...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2012.10.007
更新日期:2013-01-15 00:00:00
abstract::The neuropeptide relaxin-3 is expressed by the pontine nucleus incertus. Relaxin-3 and synthetic agonist peptides modulate arousal and cognitive processes via activation of the relaxin-family peptide 3 receptor (RXFP3). Despite the presence of RXFP3 in the nucleus of the solitary tract (NTS), the ability of RXFP3 to m...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2019.103310
更新日期:2020-01-01 00:00:00
abstract::It has been shown that tiny gas nanobubbles form spontaneously on a smooth hydrophobic surface submerged in water. These nanobubbles were shown to be the source of gas micronuclei from which bubbles evolved during decompression of silicon wafers. We suggest that the hydrophobic inner surface of blood vessels may be a ...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2013.04.013
更新日期:2013-08-01 00:00:00
abstract::Chronic heart failure (CHF) results in a greater cost of breathing and necessitates an elevated diaphragm blood flow (BF). Dietary nitrate (NO3‾) supplementation lowers the cost of exercise. We hypothesized that dietary NO3‾ supplementation would attenuate the CHF-induced greater cost of breathing and thus the heighte...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2017.09.017
更新日期:2018-01-01 00:00:00
abstract::Previous studies have demonstrated that carbonic anhydrase inhibition with acetazolamide reduces exercise capacity. The mechanism responsible for this early fatigue is unclear, but may be partly mediated by impaired respiratory muscle function. Inspiratory muscle strength and endurance were assessed in seven healthy m...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2012.08.023
更新日期:2013-01-15 00:00:00
abstract::Our aim was to investigate the role of microRNA on epithelial wound repair by global microRNA silencing. We have also analysed the influence of five miRNAs (miR-328, miR-342, miR-411, miR-609, miR-888, previously identified) on wound repair in 16HBE14o-bronchial epithelial cell line. Cells were transfected with siRNAs...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2017.03.010
更新日期:2017-08-01 00:00:00
abstract::The landmark demonstrations in 2005 that ATP released centrally during hypoxia and hypercapnia contributes to the respective ventilatory responses validated a decade-old hypothesis and ignited interest in the potential significance of P2 receptor signaling in central respiratory control. Our objective in this review i...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,评审
doi:10.1016/j.resp.2008.06.004
更新日期:2008-12-10 00:00:00
abstract::The postnatally developing lungs of the quokka wallaby, Setonix brachyurus, were investigated macroscopically and by light microscopic morphometry. Lung, parenchymal and non-parenchymal volumes as well as the components of the latter two were analysed by regression analysis. The lungs comprised a single undivided left...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/s1569-9048(02)00204-5
更新日期:2003-02-19 00:00:00
abstract::Cigarette smoke (CS)-induced emphysema is caused by a continuous inflammatory response in the lower respiratory tract. The development of the condition is believed to be mediated by oxidant-antioxidant imbalance. This paper describes the effects of long-term CS exposure on alveolar cell recruitment, antioxidant defens...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2011.03.023
更新日期:2011-07-31 00:00:00
abstract::Loss of alveolar barrier function is important in the development of pulmonary edema, but quantitation of its integrity has been difficult in the intact lung. We report a new non-radioactive method to assess paracellular and transcellular permeability of alveolar barrier in buffer-perfused rabbit lungs. Changes in alv...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/s1569-9048(02)00150-7
更新日期:2002-10-23 00:00:00
abstract::The Kölliker-Fuse Nucleus (KF) has been widely investigated for its contribution to "inspiratory off-switch" while more recent studies showed that activation of the Parabrachial Nucleus (PBN) shortened expiratory duration. This study used an adult, in vivo, decerebrate rabbit model to delineate the contribution of eac...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2020.103401
更新日期:2020-06-01 00:00:00
abstract::We have observed that pulmonary rapidly adapting receptor activity is greater in emphysematous rats than in controls. Pulmonary receptor activity, if modified by lung disease, may produce an inappropriate drive to breathe which may be perceived as dyspnoea. To investigate the efferent (drive) component of this hypothe...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2006.08.001
更新日期:2007-04-16 00:00:00
abstract::We examined the effect of heavy-intensity 'priming' exercise on the rate of adjustment of pulmonary O2 uptake (τV˙O2p) initiated from elevated intensities. Fourteen men (separated into two groups: τV˙O2p≤25s [Fast] or τV˙O2p>25s [Slow]) completed step-transitions from 20W to 45% lactate threshold (LT; lower-step, LS) ...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2016.09.013
更新日期:2017-01-01 00:00:00
abstract::The aim of this study was to examine whether lung function and respiratory muscle strength are associated with weightlifting strength and body composition in non-athletic males. A total 51 males with resistance training experience participated. One-repetition maximum tests were performed for the bench press, squat and...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2021.103616
更新日期:2021-01-14 00:00:00