Abstract:
:Preparations of cauda epididymides were taken from rats unilaterally vasectomised by medial transection of the vas deferens. Responses of preparations from the operated side to field stimulation (10 pulses, 70 V, 1 ms, 0.1-20 Hz) declined in parallel with decreases in catecholamine fluorescence. There were leftward shifts in the mean cumulative concentration-response curves to noradrenaline on vasectomised cauda epididymides of 5-, 10-, and 12-fold at days 2, 7 and 28, respectively compared to unoperated cauda epididymides following vasectomy. Nisoxetine (0.1 mumol/l) enhanced the potency of noradrenaline upon unoperated but not upon vasectomised segments. There were leftward shifts of 7- and 8-fold in the mean cumulative concentration-response curves to acetylcholine in vasectomised cauda epididymides at days 2 and 7, respectively, following surgery. Responses to methoxamine and carbachol were unaffected by vasectomy. Thus neurotransmission to the smooth muscle of the rat cauda epididymis resembles that to the vas deferens, in that vasectomy results in the development of prejunctional supersensitivity to noradrenaline and acetylcholine.
journal_name
Eur J Pharmacoljournal_title
European journal of pharmacologyauthors
Ventura S,DeGaris RM,Pennefather JNdoi
10.1016/0014-2999(89)90315-4subject
Has Abstractpub_date
1989-12-19 00:00:00pages
227-35issue
2-3eissn
0014-2999issn
1879-0712pii
0014-2999(89)90315-4journal_volume
174pub_type
杂志文章abstract::We have developed a model of ischaemia-reperfusion injury in C57BL/6 mice involving ischaemia for 0.5 to 2.5 h with an elastic tourniquet on one hind limb and reperfusion for 24 h, analogous to a well-established model of ischaemia-reperfusion injury in the rat. Viability was assessed in tissue homogenates of the gast...
journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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