Abstract：
OBJECTIVE:To investigate the relationship between genetic factor and prostate cancer (Pca) risk and the possible cause in it. METHODS:The polymorphisms of cytochrome P450 family 17 (CYPl7) rs743572, p27 V109G and androgen receptor (AR) gene CAG repeat length in peripheral blood from 70 cases and 70 controls were detected through the polymerase chain reaction-restriction fragment length polymorphism technique or short tandem repeat-polymerase chain reaction technique. Then, according to the results of case-control study, the recombinant plasmids containing the wild/mutant p27 gene were constructed and transfected Pca LNcap cells. After 24 and 72 h of transfection, the cell proliferative activity was determined by MTT method, cell cycle distribution and apoptosis was detected by flow cytometry, and the expression level of bcl-2, caspase-3 and p27 protein was determined by Western-blot. RESULTS:In three target polymorphisms, only p27 V109G polymorphism was related to Pca risk (P = 0.030, OR = 0.202, 95% CI = 0.042-0.973). Pca risk of p27-109G allele was lower than -109V allele (P = 0.006, OR = 0.285, 95% CI = 0.110-0.737). Cells transfected with wild/mutant p27 gene both showed the higher cells apoptosis rate and the lower cell proliferative activity than mock cells (P < 0.05 or 0.01), the regulatory effect of mutant p27 on cell proliferation and apoptosis was stronger than the wild p27 (P < 0.05). CONCLUSIONS:p27-109G allele that could cause higher p27 protein expression than -109V allele in LNcap cells, maybe is the protective factor of Pca.
journal_name
Asian Pac J Trop Medjournal_title
Asian Pacific journal of tropical medicineauthors
Han QH,Shan ZJ,Hu JT,Zhang N,Zhang XPdoi
10.1016/j.apjtm.2015.06.005subject
Has Abstractpub_date
2015-07-01 00:00:00pages
569-73issue
7eissn
1995-7645issn
2352-4146pii
S1995-7645(15)00062-0journal_volume
8pub_type
杂志文章abstract：OBJECTIVE:To confirm dengue infection among Russian tourists returned from Southeast and Mexico in 2010-2013 with clinical signs of infection. METHODS:Blood and serum samples from patients were collected. NS1 antigen and human IgM/IgG antibodies to dengue virus were identified using commercial tests manufactured by "S...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60194-2
更新日期：2015-02-01 00:00:00
abstract：OBJECTIVE:To provide further evidence for the ethnomedicinal use of the Eastern Nigeria mistletoe, Loranthus micranthus (L. micranthus), as an immunostimulant. METHODS:Solvent fractions from the crude extract of the mistletoe plant was obtained and screened by the cell mediated delayed type hypersensitivity reaction (...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(11)60137-5
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVE:To determine the range and incidence of complications associated with Plasmodium vivax (P. vivax) malaria. METHODS:A retrospective analysis was performed of all patients of P. vivax malaria admitted in Kasturba Medical College, Manipal between January and December, 2010. Patients with mixed malarial infectio...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(11)60251-4
更新日期：2012-01-01 00:00:00
abstract：OBJECTIVE:To establish the prevalence and associated risk factors of Schistosoma mansoni (S. mansoni) infection among schoolchildren at a village in Wolaita Zone, Southern Ethiopia. METHODS:A cross-sectional study was carried out among primary schoolchildren. A total of 384 randomly selected study subjects provided st...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2015.05.009
更新日期：2015-06-01 00:00:00
abstract：OBJECTIVE:To identify the Leishmania species in infected sand flies by Real-time PCR coupled with HRM analysis. METHODS:Real-time PCR coupled with HRM analysis targeting the first internal transcribed spacer (ITS1) of nuclear ribosomal DNA as the genetic marker was used to identify and distinguish Leishmania species i...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60002-X
更新日期：2014-02-01 00:00:00
abstract：OBJECTIVE:To estimate the genetic diversity of Kokobera virus, the date of origin and the spread among different viruses in the endemic regions of Australia. METHODS:Two datasets were built. The first consisting of 29 sequences of the NS5/3' UTR region of Kokobera group downloaded from GenBank, the second including on...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2016.07.028
更新日期：2016-10-01 00:00:00
abstract：OBJECTIVE:To investigate the role of human host heme-oxygenase-1 (HO-1) in pathogenesis of cerebral malaria in the in vitro model. METHODS:The effect of human host HO-1 [human brain microvascular endothelial cell (HBMEC)] on hemoglobin degradation in the co-culture model of HBMEC and ITG Plasmodium falciparum-infected...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2016.11.011
更新日期：2017-01-01 00:00:00
abstract：OBJECTIVE:To report presence of Leishmania major in Khyber Pakhtunkhwa of Pakistan, where cutaneous leishmaniasis (CL) is endemic and was thought to be caused by Leishmania tropica only. METHODS:Biopsy samples from 432 CL suspected patients were collected from 3 southern districts of Khyber Pakhtunkhwa during years 20...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2017.07.015
更新日期：2017-07-01 00:00:00
abstract：OBJECTIVE:To explore the relationship between IL-1β, IL-1Ra gene polymorphism and the occurrence of polycystic ovary syndrome (PCOS) infertility. METHODS:A total of 59 PCOS infertility cases visiting the reproductive center of our hospital from Mar. 2010 to Mar. 2012 and 56 healthy women were selected. ELISA method wa...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(13)60030-9
更新日期：2013-03-01 00:00:00
abstract：OBJECTIVE:To identify the possible antiplasmodial compounds from leaf, stem, root and flower extracts of Ocimum canum (O. canum), Ocimum sanctum (O. sanctum) and Ocimum basilicum (O. basilicum). METHODS:The O. canum, O. sanctum and O. basilicum were collected from Ramanathapuram District, Tamil Nadu and the extraction...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(12)60004-2
更新日期：2012-02-01 00:00:00
abstract：OBJECTIVE:To investigate the expression and significance of MMP-3 in synovium of knee joint at different stage in osteoarthritis (OA) patients. METHODS:Knee synovial tissue were collected in 90 OA patients (the OA group). Patients in the OA group was divided into 3 subgroups: grade I subgroup (n=30), grade II subgroup...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60042-0
更新日期：2014-04-01 00:00:00
abstract：:A 19-year-old Asian Indian female presented with productive cough since the past one month and low grade fever since the past two weeks. She was diagnosed with pulmonary tuberculosis and treated with antitubercular drugs. Subsequently, delayed cultures of bronchoalveolar lavage fluid grew Burkholderia pseudomallei (B....
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60031-6
更新日期：2014-03-01 00:00:00
abstract：OBJECTIVE:To delineate the frequency of occurrence of bla(CTX-M), bla(TEM), and bla(SHV) in Enterobacteriaceae from North-Indian tertiary hospital. METHODS:A random collection of a subset of 45 Escherichia coli (E. coli) and 28 Klebsiella pneumoniae (K. pneumoniae) that was resistant to a third generation cephalospori...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(11)60046-1
更新日期：2011-02-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of ischemic precondition to protect ischemia-reperfusion injury and reduce IL-6 expression in the rats liver transplantation. METHODS:The rat portal vein infusion of autologous liver transplantation model were used. The rats were divided into ischemic preconditioning rats liver tran...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(13)60045-0
更新日期：2013-05-13 00:00:00
abstract：OBJECTIVE:To evaluate the antioxidant activity of aqueous extract of Moringa oleifera (M. oleifera) young leaves by in vivo as well as in vitro assays. METHODS:In vitro study included estimation of total phenolic, total flavonol, total flavonoid and total antioxidant power (FRAP assay). In addition, in vivo study was ...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(13)60068-1
更新日期：2013-06-01 00:00:00
abstract：OBJECTIVE:To develop attenuated strains of Salmonella enterica serovar Typhi (S. typhi) for the candidate vaccine by osmolar stress. METHODS:S. typhi SS3 and SS5 strains were isolated from asymptomatic typhoid carriers in Namakkal, Tamil Nadu, India. Both strains were grown in LB (Luria Bertani) medium supplemented wi...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60165-6
更新日期：2014-12-01 00:00:00
abstract：OBJECTIVE:To study the inhibitory effect of matrine on bladder cancer cell growth and invasion in vitro through PI3K/AKT signaling pathway. METHODS:Human T24 bladder cancer cell lines were cultured and treated with different doses of matrine (0.25 mg/mL, 0.5 mg/mL and 1.0 mg/mL) as well as 20 μmol/L PI3K inhibitor LY2...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2017.05.009
更新日期：2017-05-01 00:00:00
abstract：OBJECTIVE:To study the correlation of lipid ratios and individual lipid indexes of patients with type 2 diabetes with glycosylated hemoglobin (HbA(1c)). METHODS:Samples were collected from 128 type 2 diabetic patients (aged 19-90 years; male 72, female 56). The sera were analyzed for HbA(1c), total cholesterol (TC), t...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(12)60080-7
更新日期：2012-06-01 00:00:00
abstract：OBJECTIVE:To determine the phytochemical composition, antioxidant and anticancer activities of ethanolic and water leaves extracts of Annona muricata (A. muricata) from the Eastern Uganda. METHODS:Phytochemical screening was conducted using standard qualitative methods and a Chi-square goodness of fit test was used to...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60258-3
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVE:To investigate the biosorption potential of isolated bacteria as an alternative biosorbent material for the removal of zinc and nickel from aqueous solution in a bubble column bioreactor. METHORDS:In this study from four points of waste water treatment plant, some Gram-positive and Gram-negative bacteria und...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60231-5
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVE:To investigate the composition of volatile compounds in the different accessions of Perilla frutescens (P. frutescens) collected from various habitats of China and Japan. METHODS:In the present study, the essential oil from the leaves of P. frutescens cultivars from China and Japan was extracted by hydro-dis...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2017.07.004
更新日期：2017-07-01 00:00:00
abstract：OBJECTIVE:To investigate the preventive and therapeutic effect and mechanism of simvastatin on secondary inflammatory damage of rats with cerebral hemorrhage. METHODS:Sixty SD rat aged 9-12 weeks were chosen and divided into the control group, model group and simvastatin-treated group randomly with 20 rats in each gro...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2017.01.003
更新日期：2017-02-01 00:00:00
abstract：OBJECTIVE:To screen, identify, and compare the serum biomarkers between anovulatory dysfunctional uterine bleeding (ADUB) and ovulatory dysfunctional uterine bleeding (ODUB) in Lizu females. METHODS:The subjects included 128 ADUB patients, 63 ODUB patients, and 93 controls. The serum and supernate of the subjects' men...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60012-2
更新日期：2014-02-01 00:00:00
abstract：OBJECTIVE:To investigate antiviral activity of maca to reduce viral load in Madin-Darby canine kidney (MDCK) cells infected with influenza type A and B viruses (Flu-A and Flu-B, respectively). METHODS:Maca were extracted with methanol (1:2, v/v). The cell viability and toxicity of the extracts were evaluated on MDCK c...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60268-6
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVE:To study the biocompatibility and neovascularization of the PLGA nanospheres wrapped with vascular endothelial growth factor (VEGF), which can improve bladder acellular matrix graft (BAMG) with local continuous release of VEGF. METHODS:A total of 18 rabbit model (length of stenosis: 3 cm) with anterior ureth...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(13)60078-4
更新日期：2013-06-01 00:00:00
abstract：OBJECTIVE:To study the involvement of variations in 4 genes associated with susceptibility and/or protection against HIV-1 in serodiscordant couples in Burkina Faso, namely, genes encoding HLA-B57, interferon regulatory factor 1 (IRF1), dendritic cell-specific ICAM3-grabbing nonintegrin (DC-SIGN) and CCR5 delta 32 (CCR...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60211-X
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVE:To elucidates the immunoprophylactic potential of glutathion-s-transferase (GST) from cattle filarial parasite Setaria digitata (S. digitata) against lymphatic filariasis. METHODS:GST was purified through affinity chromatography (SdGST) and chacterized by SDS-PAGE and Nano-LC MS/MS analysis. Antibody isotype...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(11)60066-7
更新日期：2011-03-01 00:00:00
abstract：OBJECTIVE:To investigate the propensity of plumbagin to inhibit the three isoforms of human cytochrome P450 (CYP), i.e., CYP1A2, CYP2C19, and CYP3A4 using human liver microsomes in vitro. METHODS:Inhibitory effects of plumbagin on the three human CYP isoforms were investigated using pooled human liver microsomes. Phen...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2015.10.016
更新日期：2015-11-01 00:00:00
abstract：:Salmonella are most commonly associated with gastroenteritis and enteric fever in humans. Occasionally, dissemination of bacilli throughout the body results in establishment of localized foci of persistent infection especially in patients with debilitating diseases and immunosuppressive states. Infection at various ab...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(11)60117-X
更新日期：2011-05-01 00:00:00
abstract：OBJECTIVE:To evaluate the anti-inflammatory, analgesic, antioxidant and acute toxicity of extracts obtained from a successive extraction with solvents of ascending polarity [hexane, hex; chloroform, CHCl3 and ethanol (EtOH)] of Ternstroemia sylvatica Schltdl. & Cham. METHODS:The antioxidant potential was evaluated by ...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2017.10.007
更新日期：2017-11-01 00:00:00
