Effect of thymosin α₁ on the phenotypic and functional maturation of dendritic cells from children with acute lymphoblastic leukemia.

Abstract:

:To determine the effect of thymosin α1 (Tα1) on the phenotypic and functional maturation of HL‑60 cells, freeze‑thaw antigen‑loaded dendritic cells (DCs) were derived from peripheral blood mononuclear cells (PBMCs) of children with acute lymphoblastic leukemia (ALL). The DCs were generated from the PBMC samples that were collected from the PB of 10 consecutive ALL children. On day 3 of culturing, the cells in the antigen + no Tα1 (AN) and antigen + Tα1 (AT) groups were incubated with 100 µl lysates obtained from freeze‑thaw cycling. After 5 days of incubation, the AT group was administered with 100 ng/ml Tα1. On day 8, the DCs were stained with fluorescein isothiocyanate‑conjugated cluster of differentiation (CD)1a, CD83 and HLA‑DR antibodies and analyzed by flow cytometry. In addition, the killing activity of cytotoxic T lymphocytes (CTLs) from the different groups on wild‑type leukemia cells was measured. The DCs in the AT group exhibited more apparent, characteristic dendritic morphologies than the control and AN group DCs. Furthermore, the lowest expression level of CD1a, and the highest expression of CD83 and HLA‑DR were observed in the AT group when compared with the AN and control groups (P<0.05). The lactate dehydrogenase release assay demonstrated that the killing rate of CTL in the AT group was significantly higher than that in the control and AN groups (P<0.01). Thus, Tα1 may markedly promote the phenotypic and functional maturation of DCs, and may serve as a suitable immunomodulator of DC‑based immunotherapy for treatment of hematological malignancies.

journal_name

Mol Med Rep

authors

Li X,Liu X,Zhao Y,Zhong R,Song A,Sun L

doi

10.3892/mmr.2015.4153

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

6093-7

issue

4

eissn

1791-2997

issn

1791-3004

journal_volume

12

pub_type

杂志文章
  • A correlation analysis of miRNA‑34a and its predicted target genes in leukemia.

    abstract::microRNA‑34a (miRNA‑34a) plays an important role in the pathogenesis of leukemia. This study aimed to explore its role in the proliferation of HL‑60 cells and the correlation with some of its predicted target genes: the cyclin‑dependent kinase 4 (CDK4), the oncogene MYB and the silent information regulator 1 (SIRT1). ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.1931

    authors: Tang R,Li J,Yue M,Liu Z,Feng S,Tang S,Wang T

    更新日期:2014-04-01 00:00:00

  • Puerarin restores the autophagic flux to alleviate cadmium‑induced endoplasmic reticulum stress in NRK‑52E cells.

    abstract::Cadmium (Cd) is a heavy metal that can accumulate and cause damage to a variety of tissues and organs. The kidney is the primary target organ for Cd accumulation and toxic damage. Autophagy, which is a critical intracellular process, serves an important role in maintaining the homeostasis of the intracellular environm...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11301

    authors: Liu G,Zhang K,Dong W,Tan Y,Long M,Zou H,Liu Z

    更新日期:2020-09-01 00:00:00

  • 2D-1H proton magnetic resonance spectroscopic imaging study on brain metabolite alterations in patients with diabetic hypertension.

    abstract::The aim of the present study was to investigate the possible metabolic alterations in the frontal cortex and parietal white matter in patients with diabetic hypertension (DHT) using proton magnetic resonance (MR) spectroscopic imaging. A total of 33 DHT patients and 30 healthy control subjects aged between 45 and 75 w...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3305

    authors: Cao Z,Ye BD,Shen ZW,Cheng XF,Yang ZX,Liu YY,Wu RH,Geng K,Xiao YY

    更新日期:2015-06-01 00:00:00

  • The protective effect of curcumin against the 19‑kDa Mycobacterium tuberculosis protein-induced inflammation and apoptosis in human macrophages.

    abstract::The Mycobacterium tuberculosis 19-kDa lipoprotein (P19) is both cell wall-associated and secreted and is a candidate virulence factor that could cause the apoptosis of human macrophages infected with M. tuberculosis. P19 induces TLR2 activation, resulting in the upregulation of death receptors and ligands, followed by...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2615

    authors: Li M,Wu Z,Niu W,Wan Y,Zhang L,Shi G,Xi X

    更新日期:2014-12-01 00:00:00

  • MUC1 and maltose‑binding protein recombinant fusion protein combined with Bacillus Calmette‑Guerin induces MUC1‑specific and nonspecific anti‑tumor immunity in mice.

    abstract::Human mucin 1 (MUC1) is a target for immunotherapy. The major problem associated with MUC1‑based cancer vaccines is the weakness of the immunogenicity of MUC1. The present study aimed to develop an efficient cancer vaccine through generating a recombinant fusion protein consisting of MUC1 and maltose‑binding protein (...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2306

    authors: Fang F,Ma J,Ni W,Wang F,Sun X,Li Y,Li Q,Xie F,Wang J,Zhai R,Liu Z,Gao S,Tai G

    更新日期:2014-08-01 00:00:00

  • Hepatitis B virus X protein upregulates DNA methyltransferase 3A/3B and enhances SOCS-1CpG island methylation.

    abstract::The aim of the present study was to investigate the effect of hepatitis B virus X protein (HBx) on the expression of DNA methyltransferase (DNMT)3A/3B and suppressors of cytokine signaling‑1 (SOCS‑1), as well as promoter CpG island methylation of the SOCS‑1 gene. Stable hepatocyte cell lines expressing the HBx gene (p...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4545

    authors: Fu X,Song X,Li Y,Tan D,Liu G

    更新日期:2016-01-01 00:00:00

  • Fas expression is downregulated in gastric cancer.

    abstract::The aim of the present study was to investigate Fas expression in tumor samples from patients with gastric cancer, in order to determine the involvement of the Fas signaling pathway. The protein expression levels of Fas, caspase-8, caspase‑3 and poly (adenosine diphosphate‑ribose) polymerase 1 (PARP1) were examined in...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.6037

    authors: Wang X,Fu Z,Chen Y,Liu L

    更新日期:2017-02-01 00:00:00

  • Degradation and removal of soybean allergen in Japanese soy sauce.

    abstract::Soy sauce is a traditional fermented seasoning of Japan and is available throughout the world. The two main raw ingredients of soy sauce are soybean and wheat, both of which are established food allergens. The present study examined the degradation and removal of soybean allergens in soy sauce by immunoblotting with a...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6815

    authors: Magishi N,Yuikawa N,Kobayashi M,Taniuchi S

    更新日期:2017-08-01 00:00:00

  • miRNA‑195 suppresses cell proliferation of ovarian cancer cell by regulating VEGFR2 and AKT signaling pathways.

    abstract::The present study aimed to investigate the functional effects of microRNA‑195 on ovarian cancer cells and the underling mechanism involved. Reverse transcription‑quantitative polymerase chain reaction was used to measure the expression of microRNA‑195 in patients with ovarian cancer. Cell proliferation and apoptosis w...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9098

    authors: Chen J

    更新日期:2018-08-01 00:00:00

  • Targeted next-generation sequencing identification of a novel missense mutation of the SKIV2L gene in a patient with trichohepatoenteric syndrome.

    abstract::Trichohepatoenteric syndrome (THES) is a rare autosomal, recessively inherited disorder. Mutations in the tetratricopeptide repeat domain 37 (TTC37) gene and the superkiller viralicidic activity 2‑like (SKIV2L) gene have been identified to cause THES. The present study reported a case of a Chinese boy, who presented c...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5503

    authors: Zheng B,Pan J,Jin Y,Wang C,Liu Z

    更新日期:2016-09-01 00:00:00

  • Inhibition of 6-hydroxydopamine-induced endoplasmic reticulum stress by sulforaphane through the activation of Nrf2 nuclear translocation.

    abstract::Endoplasmic reticulum (ER) stress plays a key role in the development of neurodegenerative diseases, including Parkinson's disease (PD). Sulforaphane (SF) is a natural drug derived from isothiocyanate found in cruciferous vegetables. Although there are reports indicating that SF is ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.894

    authors: Deng C,Tao R,Yu SZ,Jin H

    更新日期:2012-07-01 00:00:00

  • Curcumin improves diabetes mellitus‑associated cerebral infarction by increasing the expression of GLUT1 and GLUT3.

    abstract::Curcumin is characterized by anti‑inflammatory, anti‑oxidative, antiviral, antifibrotic, anticoagulation and glucose regulatory functions. However, whether it is protective in diabetes mellitus‑associated cerebral infarction remains to be fully elucidated. In the present study, it was demonstrated for the first time, ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.8085

    authors: Xia M,Ye Z,Shi Y,Zhou L,Hua Y

    更新日期:2018-01-01 00:00:00

  • Targeting strategies of adenovirus‑mediated gene therapy and virotherapy for prostate cancer (Review).

    abstract::Prostate cancer (PCa) poses a high risk to older men and it is the second most common type of male malignant tumor in western developed countries. Additionally, there is a lack of effective therapies for PCa at advanced stages. Novel treatment strategies such as adenovirus‑mediated gene therapy and virotherapy involve...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章,评审

    doi:10.3892/mmr.2017.7487

    authors: Cai Z,Lv H,Cao W,Zhou C,Liu Q,Li H,Zhou F

    更新日期:2017-11-01 00:00:00

  • Intermittent fasting increases SOD2 and catalase immunoreactivities in the hippocampus but does not protect from neuronal death following transient ischemia in gerbils.

    abstract::Intermittent fasting has been shown to have neuroprotective effects against transient focal cerebral ischemic insults. However, the effects of intermittent fasting on transient global ischemic insult has not been studied much yet. The present study examined effects of intermittent fasting on endogenous antioxidant enz...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9503

    authors: Ahn JH,Noh Y,Shin BN,Kim SS,Park JH,Lee TK,Song M,Kim H,Lee JC,Yong JH,Kang IJ,Lee YL,Won MH,Kim JD

    更新日期:2018-12-01 00:00:00

  • Synthesis of regioselectively acylated quercetin analogues with improved antiplatelet activity.

    abstract::The aim of the present study was to report on a complete synthetic approach, namely benzylation-hydrolysis-acylation‑hydrogenation, to the synthesis of regioselectively acylated quercetin analogues using low‑cost rutin as a starting material. Three quercetin analogues, quercetin‑3‑O‑propionate (Q‑pr), quercetin‑3‑O‑bu...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7781

    authors: Duan Y,Sun N,Xue M,Wang X,Yang H

    更新日期:2017-12-01 00:00:00

  • Fine particulate matter aggravates allergic airway inflammation through thymic stromal lymphopoietin activation in mice.

    abstract::Fine particulate matter (PM2.5) has been linked to exacerbation of allergic airway inflammation in mice. However, the mechanism underlying exposure to PM2.5 and subsequent and adverse effects remains to be fully elucidated. Therefore, the present study aimed to investigate the effects of PM2.5 by different levels on a...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7089

    authors: Liu Y,Feng GZ,Du Q,Jin XX,Du XR

    更新日期:2017-10-01 00:00:00

  • Gli affects the stemness and prognosis of epithelial ovarian cancer via homeobox protein NANOG.

    abstract::Gli proteins are key transcription factors of the Hedgehog (HH) signaling pathway, which is associated with tumorigenesis and drug resistance. However, the role of the HH signaling pathway in epithelial ovarian cancer (EOC) remains unclear. Studies have demonstrated that in some tumors, homeobox protein NANOG (NANOG),...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11767

    authors: Zhao H,Li N,Pang Y,Zhao J,Wu X

    更新日期:2021-02-01 00:00:00

  • Blockade of vascular endothelial growth factor-A/receptor 2 exhibits a protective effect on angiotensin-II stimulated podocytes.

    abstract::Vascular endothelial growth factor (VEGF) and Angiotensin II (Ang-II) are important in glomerulosclerosis, which is one of the main causes of chronic kidney disease. Previous studies have demonstrated that angiotensin type 1 receptor blocker (ARB) can inhibit the synthesis of VEGF mediated by Ang‑II and can effectivel...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3911

    authors: Liu X,Zhang H,Wang Q,Yu K,Wang R,Sun J

    更新日期:2015-09-01 00:00:00

  • Curcumin increases cholesterol efflux via heme oxygenase‑1‑mediated ABCA1 and SR‑BI expression in macrophages.

    abstract::Curcumin, which is an extract from a traditional Chinese medicine, has previously been demonstrated to exhibit an anti‑atherosclerotic effect, which is closely associated with an increase in cholesterol efflux. However, it is unclear as to whether the increased effect is mediated by heme oxygenase (HO)‑1. Macrophages ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.8577

    authors: Zhong Y,Feng J,Fan Z,Li J

    更新日期:2018-04-01 00:00:00

  • Lymphotoxin β receptor activation promotes bladder cancer in a nuclear factor-κB-dependent manner.

    abstract::Bladder cancer (BCa) is the most common tumor of the urinary system. Chronic inflammation in the papillary urothelial neoplasm of low malignant potential (PUNLMP)may contribute to carcinogenesis, including that of BCa, via poorly understood mechanisms. In this study, we show that the lymphotoxin β receptor (LTβR) is u...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2826

    authors: Shen M,Duan X,Zhou P,Zhou W,Wu X,Xu S,Chen Y,Tao Z

    更新日期:2015-02-01 00:00:00

  • Overexpression of activating transcription factor 3 exerts suppressive effects in HepG2 cells.

    abstract::The present study observed and compared the biological behaviour of HepG2 cells prior and subsequent to the overexpression of activating transcription factor 3 (ATF3). Experiments investigating the cytological function by which ATF3 affects liver cancer cells were also performed. MTT, Transwell and flow cytometry assa...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9707

    authors: Li X,Zang S,Cheng H,Li J,Huang A

    更新日期:2019-02-01 00:00:00

  • Identification of key genes implicated in the suppressive function of human FOXP3+CD25+CD4+ regulatory T cells through the analysis of time‑series data.

    abstract::Human forkhead box P3 (FOXP3)+ cluster of differentiation (CD)25+CD4+ regulatory T cells (Tregs) are a type of T cell that express CD4, CD25 and FOXP3, which are critical for maintaining immune homeostasis. The present study aimed to determine the mechanisms underlying Treg function. The GSE11292 dataset was downloade...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.8366

    authors: Bai X,Shi H,Yang M,Wang Y,Sun Z,Xu S

    更新日期:2018-03-01 00:00:00

  • Activation of corticotropin-releasing factor receptor 1 aggravates dextran sodium sulphate-induced colitis in mice by promoting M1 macrophage polarization.

    abstract::The corticotropin-releasing factor (CRF) family is involved in modulating gastrointestinal motility, sensitivity and inflammation. CRF signalling exerts an important role in inflammatory bowel disease (IBD), predominantly by activating CRF receptors. The aim of the present study was to investigate the function of CRF ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7909

    authors: Chen H,Shi H,Liu Y,Ren X,He S,Chang X,Yin Y

    更新日期:2018-01-01 00:00:00

  • Paclitaxel inhibits selenoprotein S expression and attenuates endoplasmic reticulum stress.

    abstract::The primary effect of the endoplasmic reticulum (ER) stress response or unfolded protein response (UPR) is to reduce the load of unfolded protein and promote survival. However, prolonged and severe ER stress leads to tissue injury and serious diseases. Thus, it is important to identify drugs that can attenuate ER stre...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5152

    authors: Qin HS,Yu PP,Sun Y,Wang DF,Deng XF,Bao YL,Song J,Sun LG,Song ZB,Li YX

    更新日期:2016-06-01 00:00:00

  • MicroRNA‑744 inhibits tumor cell proliferation and invasion of gastric cancer via targeting brain‑derived neurotrophic factor.

    abstract::Gastric cancer is the fourth most common malignancy and the third leading cause of cancer‑associated deaths worldwide. It has previously been demonstrated that microRNAs (miRNAs) are actively involved in the pathogenesis of gastric cancer. Therefore, miRNAs have been proposed as promising therapeutic targets in gastri...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7167

    authors: Xu AJ,Fu LN,Wu HX,Yao XL,Meng R

    更新日期:2017-10-01 00:00:00

  • Analysis of changes in microRNA expression profiles in response to the troxerutin-mediated antioxidant effect in human dermal papilla cells.

    abstract::Dermal papilla (DP) cells function as important regulators of the hair growth cycle. The loss of these cells is a primary cause of diseases characterized by hair loss, including alopecia, and evidence has revealed significantly increased levels of reactive oxygen species (ROS) in hair tissue and DP cells in the baldin...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3717

    authors: Lim KM,An S,Lee OK,Lee MJ,Lee JP,Lee KS,Lee GT,Lee KK,Bae S

    更新日期:2015-08-01 00:00:00

  • Correlation between oxidative stress and the NF-κB signaling pathway in the pulmonary tissues of obese asthmatic mice.

    abstract::The obesity-asthma phenotype is characterized by increased asthma severity and decreased glucocorticoid responsiveness. To date, the mechanism underlying the association between obesity and asthma remain to be fully elucidated. The present study investigated the correlation between oxidative stress and the nuclear fac...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4663

    authors: Liu X,Lin R,Zhao B,Guan R,Li T,Jin R

    更新日期:2016-02-01 00:00:00

  • Hepatitis B virus inhibits the expression of CD82 through hypermethylation of its promoter in hepatoma cells.

    abstract::The tumor suppressor gene CD82, also known as KAI1, may act as a general suppressor of metastasis in numerous types of cancer. It is hypothesized that downregulation of CD82 gene expression may be an important factor in the induction of hepatocellular carcinoma (HCC), however the mechanism for this requires further st...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2495

    authors: Yu G,Bing Y,Li W,Xia L,Liu Z

    更新日期:2014-11-01 00:00:00

  • Ketamine‑induced bladder dysfunction is associated with extracellular matrix accumulation and impairment of calcium signaling in a mouse model.

    abstract::Due to the rising abuse of ketamine usage in recent years, ketamine‑induced urinary tract syndrome has received increasing attention. The present study aimed to investigate the molecular mechanism underlying ketamine‑associated cystitis in a mouse model. Female C57BL/6 mice were randomly divided into two groups: One g...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.9907

    authors: Shen CH,Wang ST,Wang SC,Lin SM,Lin LC,Dai YC,Liu YW

    更新日期:2019-04-01 00:00:00

  • Cathepsin S is aberrantly overexpressed in human hepatocellular carcinoma.

    abstract::Several lysosomal cathepsins have been implicated in a number of diseases, from arthritis to cancer. A recent member of the cathepsin family, cathepsin S (Cat S) has been associated with several types of cancer in humans. However, to date, no report has linked Cat S to human hepatocellular carcinoma (HCC). Here, we in...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr_00000161

    authors: Xu J,Li D,Ke Z,Liu R,Maubach G,Zhuo L

    更新日期:2009-09-01 00:00:00