Paired analysis of the microbiota of surface mucus and whole-tissue specimens in patients with chronic rhinosinusitis.

Abstract:

BACKGROUND:The role of bacteria in the pathogenesis of chronic rhinosinusitis (CRS) remains uncertain. Recent evidence suggests that bacteria are able to establish microcolonies within the underlying mucosa. However, to date there has been no systematic comparison of bacterial community composition and diversity in the surface mucosa with that of the underlying tissue. METHODS:Paired swabs and whole-tissue samples were collected from the middle meatus of 9 patients with CRS undergoing endoscopic sinus surgery. The bacterial composition and diversity of the samples were determined using 16S rRNA gene amplicon pyrosequencing. RESULTS:The bacterial communities of both swabs and tissues were dominated by known residents of the sinonasal cavity such as Staphylococcus, Corynebacterium, Prevotella, and Peptoniphilus. Although bacterial diversity (richness) did not differ between the 2 groups of samples, there were significant differences in the composition of bacterial communities. Molecular analyses revealed a large amount of interpersonal variation between patients. CONCLUSION:Swab and tissue samples revealed similar bacterial diversity to each other and to that of other microbiota studies reported in the CRS literature. However, bacterial composition was significantly different between the 2 sample types, even though the tissue biopsies also comprise bacteria from the surface. We speculate that the bacteria on the surface seed the underlying tissue via the damaged epithelium in CRS patients, which over time develops into a distinct bacterial community.

authors

Kim RJ,Biswas K,Hoggard M,Taylor MW,Douglas RG

doi

10.1002/alr.21600

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

877-83

issue

10

eissn

2042-6976

issn

2042-6984

journal_volume

5

pub_type

杂志文章
  • Association between allergic rhinitis and poor sleep parameters in U.S. adults.

    abstract:BACKGROUND:Evidence suggests relationships between allergic rhinitis (AR) and poor sleep parameters, but population-based studies in the United States are lacking. This study investigates the relationship between AR and sleep parameters in a representative sample of U.S. adults. METHODS:Cross-sectional study of 5563 p...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.22174

    authors: Roxbury CR,Qiu M,Shargorodsky J,Lin SY

    更新日期:2018-10-01 00:00:00

  • Evidence of microbiota dysbiosis in chronic rhinosinusitis.

    abstract:BACKGROUND:Despite considerable research, the pathogenesis of chronic rhinosinusitis (CRS) remains poorly understood. Potential microbial roles in the etiology or progression of CRS have long been hypothesized, yet few specific associations have been identified. In this study we investigate associations between pattern...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.21871

    authors: Hoggard M,Biswas K,Zoing M,Wagner Mackenzie B,Taylor MW,Douglas RG

    更新日期:2017-03-01 00:00:00

  • Ossifying fibroma of the nose and paranasal sinuses.

    abstract:BACKGROUND:The purpose of this work was to perform a systematic review regarding ossifying fibroma and its multiple variants of the paranasal sinuses, and to identify any clinical differences between the multiple variants. METHODS:A search of the U.S. National Library of Medicine (PubMed) database was performed for th...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章,评审

    doi:10.1002/alr.21067

    authors: Manes RP,Ryan MW,Batra PS,Mendelsohn D,Fang YV,Marple BF

    更新日期:2013-02-01 00:00:00

  • Impact of chronic rhinosinusitis on sleep: a controlled clinical study.

    abstract:BACKGROUND:Earlier studies have suggested that patients with chronic rhinosinusitis (CRS) report worse sleep quality than population norms. What remains unknown is whether these patients are actually experiencing measurable changes in objective sleep parameters. The goal of this study was to prospectively evaluate obje...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章,多中心研究

    doi:10.1002/alr.22212

    authors: Alt JA,Ramakrishnan VR,Platt MP,Schlosser RJ,Storck T,Soler ZM

    更新日期:2019-01-01 00:00:00

  • An update on attitudes and use of image-guided surgery.

    abstract:BACKGROUND:The aim of this study was to compare 2010 access to, usage of, and attitudes toward image-guided surgery (IGS) for endoscopic sinus surgery and anterior skull-base surgery to those in 2005. METHODS:A mail survey of American Rhinologic Society (ARS) members was performed in January 2010. Results were compare...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.20107

    authors: Justice JM,Orlandi RR

    更新日期:2012-03-01 00:00:00

  • Evaluation of the olfactory bulb volume and olfactory threshold in patients with nasal polyps and impact of functional endoscopic sinus surgery: a longitudinal study.

    abstract:BACKGROUND:Debate still persists on the relation between olfactory bulb volume (OBV) and olfactory function. Many studies suggest that olfactory deprivation decreases the OBV. The aim of this study was to compare the olfactory threshold and OBV of patients with nasal polyps to healthy individuals and to evaluate the im...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.21478

    authors: Sadeghi M,Amali A,Ezabadi SR,Motiee-Langroudi M,Farshchi S,Mokhtari Z

    更新日期:2015-04-01 00:00:00

  • Human sinonasal epithelial cells direct dendritic function and T-cell T helper 1/T helper 2 skewing following Aspergillus exposure.

    abstract:BACKGROUND:In lower airway disease such as asthma, epithelial cells have been shown to be potent regulators of dendritic cell (DC) functions. However, it is unclear how human sinonasal epithelial cells (HSNECs) from patients with sinusitis regulate DC functions. Therefore, in these studies we investigated the ability o...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.20055

    authors: Mulligan JK,Mulligan RM,Atkinson C,Schlosser RJ

    更新日期:2011-07-01 00:00:00

  • Perioperative management in endoscopic endonasal skull-base surgery: a survey of the North American Skull Base Society.

    abstract:BACKGROUND:The objective of this work was to better understand variations in perioperative management in endoscopic endonasal skull-base surgery (EESBS) and to identify trends in management based upon the practice patterns of North American Skull Base Society (NASBS) members. METHODS:A 29-question survey evaluating pe...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.22066

    authors: Roxbury CR,Lobo BC,Kshettry VR,D'Anza B,Woodard TD,Recinos PF,Snyderman CH,Sindwani R

    更新日期:2018-05-01 00:00:00

  • Comparison of intranasal fluticasone spray, budesonide atomizer, and budesonide respules in patients with chronic rhinosinusitis with polyposis after endoscopic sinus surgery.

    abstract:BACKGROUND:Nasal steroids are a critical part of the management of patients with chronic rhinosinusitis with nasal polyposis (CRSwNP) after endoscopic sinus surgery (ESS). Increasingly, practitioners are using budesonide respules delivered to the sinonasal cavities, which is an off-label use, in lieu of traditional nas...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/alr.21688

    authors: Neubauer PD,Schwam ZG,Manes RP

    更新日期:2016-03-01 00:00:00

  • Asthma treatments: new and emerging therapies.

    abstract:BACKGROUND:As part of this primer, this article is intended to introduce new and emerging therapies for treating asthma. METHODS:A PubMed search was performed for the years 2013 through 2015 for asthma review, asthma treatment, asthma therapy, and asthma new. Subset searches were additionally performed as suggested by...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章,评审

    doi:10.1002/alr.21611

    authors: Calhoun KH

    更新日期:2015-09-01 00:00:00

  • Immunology.

    abstract:BACKGROUND:Knowledge of our immune system functions is critical for understanding allergic airway disease development as well as for selection of appropriate diagnostic and therapeutic options for patients with respiratory allergies. METHODS:This review explains the current understanding of the basic immunology of the...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章,评审

    doi:10.1002/alr.21380

    authors: Toskala E

    更新日期:2014-09-01 00:00:00

  • The efficacy of hemostatic techniques in the sheep model of carotid artery injury.

    abstract:BACKGROUND:The most dramatic complication in endonasal surgery is inadvertent injury to the internal carotid artery (ICA) with massive bleeding. Nasal packing is the favored technique for control; however, this often causes complete carotid occlusion or carotid stenosis, contributing to the morbidity and mortality of t...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.20033

    authors: Valentine R,Boase S,Jervis-Bardy J,Dones Cabral JD,Robinson S,Wormald PJ

    更新日期:2011-03-01 00:00:00

  • Allergen immunotherapy: exploring areas for further inquiry.

    abstract:BACKGROUND:Allergy-related illness impacts millions of individuals worldwide. Our objectives were to characterize current trends of clinical trials research relating to allergen immunotherapy and to describe the landscape of allergen immunotherapy in National Institutes of Health (NIH)-supported research inquiry. METH...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.22016

    authors: Ramsey T,Lai W,Svider PF,Hojjat H,Eloy JA,Folbe AJ

    更新日期:2017-12-01 00:00:00

  • Immunotherapy compliance: comparison of subcutaneous versus sublingual immunotherapy.

    abstract:BACKGROUND:Patient compliance is critical for successful allergen immunotherapy (AIT). Previous studies suggest that AIT compliance is worse outside of controlled clinical trials, with reported subcutaneous immunotherapy (SCIT) and sublingual immunotherapy (SLIT) noncompliance at 11% to 50% and 3% to 25%, respectively....

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.21699

    authors: Leader BA,Rotella M,Stillman L,DelGaudio JM,Patel ZM,Wise SK

    更新日期:2016-05-01 00:00:00

  • Back-and-forth endoscopic septoplasty: analysis of the technique and outcomes.

    abstract:BACKGROUND:To describe our clinical experience in 218 consecutive patients undergoing endoscopic back-and-forth septoplasty (EBFS), examining surgical indications, technique, and follow-up. METHODS:From January 2005 to November 2008, 218 patients underwent EBFS at the Department of Otorhinolaryngology, San Raffaele Ho...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.20100

    authors: Trimarchi M,Bellini C,Toma S,Bussi M

    更新日期:2012-01-01 00:00:00

  • Nonsteroidal anti-inflammatory drugs-exacerbated respiratory disease in adolescents.

    abstract:BACKGROUND:There has been no study investigating nonsteroidal anti-inflammatory drugs (NSAIDs)-exacerbated respiratory disease (NERD) exclusively in childhood. Therefore, in the current study, the authors aimed to evaluate the diagnostic features, clinical characteristics, and follow-up of adolescents diagnosed with NE...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.21494

    authors: Ertoy Karagol HI,Yilmaz O,Topal E,Ceylan A,Bakirtas A

    更新日期:2015-05-01 00:00:00

  • Characterization of immunoglobulin E plasma cells that are elevated in the upper airway mucosa of nonatopic patients with chronic rhinosinusitis without nasal polyps.

    abstract:BACKGROUND:The immunologic mechanisms driving inflammation in the upper airways of patients with chronic rhinosinusitis (CRS) are poorly understood. Previous studies have shown that B cells and immunoglobulin E (IgE) levels are elevated in the nasal tissue of patients with atopic chronic rhinosinusitis without nasal po...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.21696

    authors: Rashan AR,Goshn E,Peterson A,Yang Y,Phillips M,Sahaf B,Herzenberg L,Nayak JV

    更新日期:2016-04-01 00:00:00

  • International consensus statement on allergy and rhinology: allergic rhinitis-executive summary.

    abstract:BACKGROUND:The available allergic rhinitis (AR) literature continues to grow. Critical evaluation and understanding of this literature is important to appropriately utilize this knowledge in the care of AR patients. The International Consensus statement on Allergy and Rhinology: Allergic Rhinitis (ICAR:AR) has been pro...

    journal_title:International forum of allergy & rhinology

    pub_type: 共识发展会议,杂志文章

    doi:10.1002/alr.22070

    authors: Wise SK,Lin SY,Toskala E

    更新日期:2018-02-01 00:00:00

  • Impact of mentoring medical students on scholarly productivity.

    abstract:BACKGROUND:Our objectives were to evaluate collaboration with medical students and other nondoctoral authors, and assess whether mentoring such students influences the academic productivity of senior authors. METHODS:Six issues of the Laryngoscope and International Forum of Allergy & Rhinology (IFAR) were examined for...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.21247

    authors: Svider PF,Husain Q,Mauro KM,Folbe AJ,Baredes S,Eloy JA

    更新日期:2014-02-01 00:00:00

  • Patient-reported sleep outcomes lack association with mucosal eosinophilia or neutrophilia in patients with chronic rhinosinusitis undergoing functional endoscopic sinus surgery.

    abstract:BACKGROUND:Chronic rhinosinusitis (CRS) is associated with sleep dysfunction, but the underlying pathophysiology is poorly understood. The purpose of this study was to determine if mucosal eosinophilia or neutrophilia were associated with sleep dysfunction severity or altered the improvement in sleep dysfunction follow...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.22678

    authors: Farrell NF,Mace JC,Sauer DA,Thomas AJ,Geltzeiler M,Detwiller KY,Alt JA,Smith TL

    更新日期:2020-08-11 00:00:00

  • Predictive factors in patients undergoing endoscopic sinus surgery for odontogenic sinusitis.

    abstract:BACKGROUND:Odontogenic sinusitis (OS) presents a therapeutic dilemma. Evidence is lacking whether dental treatment alone is sufficient or whether patients will eventually require endoscopic sinus surgery (ESS). Our aim was to identify predictors of OS patients undergoing ESS. METHODS:Retrospective review performed of ...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.21736

    authors: Mattos JL,Ferguson BJ,Lee S

    更新日期:2016-07-01 00:00:00

  • Expression of the extracellular matrix gene periostin is increased in chronic rhinosinusitis and decreases following successful endoscopic sinus surgery.

    abstract:BACKGROUND:The extracellular matrix (ECM) is a potentially important component of mucosal immunity. ECM dysregulation in chronic rhinosinusitis (CRS) is suggested by genomewide association studies identifying ECM genes as top candidates. Further support is afforded by the demonstration of increased expression of perios...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.21056

    authors: Zhang W,Hubin G,Endam LM,Al-Mot S,Filali-Mouhim A,Desrosiers M

    更新日期:2012-11-01 00:00:00

  • Sinonasal squamous cell carcinoma and the prognostic implications of its histologic variants: a population-based study.

    abstract:BACKGROUND:Variants of squamous cell carcinoma (SCC) make up 15% of all cases of SCC of the upper aerodigestive tract. There are 5 main histologic variants of SCC in the head and neck region: verrucous (VSCC), papillary (PSCC), spindle cell (sarcomatoid) (SCSC), basaloid (BSCC), and adenosquamous (ASC). Conventional si...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.21418

    authors: Vazquez A,Khan MN,Blake DM,Patel TD,Baredes S,Eloy JA

    更新日期:2015-01-01 00:00:00

  • Toll-like receptor 4-mediated expression of interleukin-32 via the c-Jun N-terminal kinase/protein kinase B/cyclic adenosine monophosphate response element binding protein pathway in chronic rhinosinusitis with nasal polyps.

    abstract:BACKGROUND:Chronic rhinosinusitis with nasal polyps (CRSwNP) is prolonged inflammation of the sinonasal mucosa. Interleukin-32 (IL-32) is involved in the pathogenesis of chronic lung inflammatory diseases. The aim of study is to compare the expression level of IL-32 in normal nasal mucosa and CRSwNP and to investigate ...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.21792

    authors: Cho JS,Kim JA,Park JH,Park IH,Han IH,Lee HM

    更新日期:2016-10-01 00:00:00

  • Activation of the rat olfactory bulb by direct ventral stimulation after nerve transection.

    abstract:BACKGROUND:The aim of this study was to demonstrate how direct electrical stimulation can activate the olfactory bulb after denervation of the olfactory nerve input. METHODS:Sprague-Dawley rats (n = 5) were anesthetized and olfactory bulbs exposed. Olfactory nerves were transected by passing a Teflon blade between the...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.22133

    authors: Coelho DH,Socolovsky LD,Costanzo RM

    更新日期:2018-05-02 00:00:00

  • What rhinologists and allergists should know about the medico-legal implications of antibiotic use: a review of the literature.

    abstract:BACKGROUND:Antibiotics are commonly used in the management of both acute rhinosinusitis and acute exacerbations of chronic rhinosinusitis. With their common use, they have become the leading medication for litigation. It is crucial for the provider to be familiar with potential complications and the medico-legal ramifi...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章,评审

    doi:10.1002/alr.21433

    authors: Poetker DM,Smith TL

    更新日期:2015-02-01 00:00:00

  • Socioeconomic status impacts postoperative productivity loss and health utility changes in refractory chronic rhinosinusitis.

    abstract:BACKGROUND:Social determinants of health can have a substantial impact on treatment outcomes. Prior study has shown that socioeconomic status influences the likelihood of improvement in quality-of-life (QOL) following endoscopic sinus surgery (ESS). However, the impact of socioeconomic factors on changes in productivit...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章,多中心研究

    doi:10.1002/alr.22374

    authors: Beswick DM,Mace JC,Soler ZM,Rudmik L,Alt JA,Smith KA,Detwiller KY,Ramakrishnan VR,Smith TL

    更新日期:2019-09-01 00:00:00

  • Sinonasal extramedullary plasmacytoma: a systematic review of 175 patients.

    abstract:BACKGROUND:This study reviews the published literature related to extramedullary sinonasal plasmacytomas. Clinical presentation, demographics, treatment, and outcomes of this uncommon disease are reported. METHODS:A systematic review of studies for sinonasal plasmacytomas from 1950 to 2012 was conducted. A PubMed data...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章,评审

    doi:10.1002/alr.21254

    authors: D'Aguillo C,Soni RS,Gordhan C,Liu JK,Baredes S,Eloy JA

    更新日期:2014-02-01 00:00:00

  • Oral antifungal therapy for chronic rhinosinusitis and its subtypes: a systematic review.

    abstract:BACKGROUND:The objective of the evidence base review was to systematically evaluate the literature to elucidate the potential role of oral antifungal therapy in management of chronic rhinosinusitis (CRS) and its subtypes. METHODS:Articles for inclusion were identified by query of appropriate search terms in the PubMed...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章,评审

    doi:10.1002/alr.20088

    authors: Thanasumpun T,Batra PS

    更新日期:2011-09-01 00:00:00

  • Does bilateral inferior turbinate reduction affect long-term quality-of-life outcomes in patients undergoing endoscopic sinus surgery?

    abstract:BACKGROUND:The objective of this study was to evaluate the impact of bilateral inferior turbinate reduction (BITR) on patient-reported quality of life (QOL) following endoscopic sinus surgery (ESS) for chronic rhinosinusitis (CRS). METHODS:This was a prospective cohort study. Patients with CRS, who were recruited from...

    journal_title:International forum of allergy & rhinology

    pub_type: 杂志文章

    doi:10.1002/alr.22300

    authors: Scangas GA,Bleier BS,Husain Q,Holbrook EH,Gray ST,Metson R

    更新日期:2019-06-01 00:00:00