TrueAllele(®) Genotype Identification on DNA Mixtures Containing up to Five Unknown Contributors.

Abstract:

:Computer methods have been developed for mathematically interpreting mixed and low-template DNA. The genotype modeling approach computationally separates out the contributors to a mixture, with uncertainty represented through probability. Comparison of inferred genotypes calculates a likelihood ratio (LR), which measures identification information. This study statistically examined the genotype modeling performance of Cybergenetics TrueAllele(®) computer system. High- and low-template DNA mixtures of known randomized composition containing 2, 3, 4, and 5 contributors were tested. Sensitivity, specificity, and reproducibility were established through LR quantification in each of these eight groups. Covariance analysis found LR behavior to be relatively invariant to DNA amount or contributor number. Analysis of variance found that consistent solutions were produced, once a sufficient number of contributors were considered. This study demonstrates the reliability of TrueAllele interpretation on complex DNA mixtures of representative casework composition. The results can help predict an information outcome for a DNA mixture analysis.

journal_name

J Forensic Sci

authors

Perlin MW,Hornyak JM,Sugimoto G,Miller KW

doi

10.1111/1556-4029.12788

subject

Has Abstract

pub_date

2015-07-01 00:00:00

pages

857-68

issue

4

eissn

0022-1198

issn

1556-4029

journal_volume

60

pub_type

杂志文章
  • Recovery Rates of Human Fetal Skeletal Remains Using Varying Mesh Sizes.

    abstract::Human fetal skeletal elements of different gestational ages were screened with multiple mesh sizes (6.4 mm [1/4 inch], 3.2 mm [1/8 inch], 2.0 mm, and 1.0 mm) to determine their recovery rates. All remains were previously macerated, and no significantly damaged elements were used. The 6.4 mm mesh allowed a large loss o...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12922

    authors: Pokines JT,De La Paz JS

    更新日期:2016-01-01 00:00:00

  • Ribosomal ribonucleic acid (rRNA) gene typing for species identification.

    abstract::Deoxyribonucleic acid (DNA) typing of ribosomal ribonucleic acid (rRNA) genes was performed with a polymerase chain reaction (PCR) assay for species identification. A variable region of the 28S ribosomal RNA gene was amplified with primers complementary to flanking sequences phylogenetically well conserved. The produc...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Naito E,Dewa K,Ymanouchi H,Kominami R

    更新日期:1992-03-01 00:00:00

  • Metric and comparative analysis of sexual dimorphism in the Thai femur.

    abstract::Identification of sex from the skeleton is an important demographic assessment in medicolegal investigations. Studies have demonstrated that populations differ from each other in size and proportions and that these differences can affect the metric assessment of sex. It is therefore vital to determine if population di...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: King CA,Işcan MY,Loth SR

    更新日期:1998-09-01 00:00:00

  • Applicability of two commercially available kits for forensic identification of saliva stains.

    abstract::The RSID-saliva test and the SALIgAE-saliva test are two recently developed forensic saliva detection kits. In this study, we compared the sensitivity and the specificity of the two test kits with the Phadebas amylase test by analyzing amylases from various sources including human, animals, plants, and micro-organism....

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2008.00814.x

    authors: Pang BC,Cheung BK

    更新日期:2008-09-01 00:00:00

  • Air embolism during intercourse in pregnancy.

    abstract::Air embolism may rarely cause sudden death during or shortly after pregnancy. Certain obstetric techniques, douching procedures, and blowing air into the vagina have been associated with this phenomenon. A case of venous air embolism in a pregnant woman occurring during sexual intercourse in a rear entry position is p...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Lifschultz BD,Donoghue ER

    更新日期:1983-10-01 00:00:00

  • The role of mobile computed tomography in mass fatality incidents.

    abstract::Mobile multi-detector computed tomography (MDCT) scanners are potentially available to temporary mortuaries and can be operational within 20 min of arrival. We describe, to our knowledge, the first use of mobile MDCT for a mass fatality incident. A mobile MDCT scanner attended the disaster mortuary after a five vehicl...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2007.00548.x

    authors: Rutty GN,Robinson CE,BouHaidar R,Jeffery AJ,Morgan B

    更新日期:2007-11-01 00:00:00

  • Evaluating DNA profile evidence when the suspect is identified through a database search.

    abstract::The paper is concerned with the strength of DNA evidence when a suspect is identified via a search through a database of the DNA profiles of known individuals. Consideration of the appropriate likelihood ratio shows that in this setting the DNA evidence is (slightly) stronger than when a suspect is identified by other...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Balding DJ,Donnelly P

    更新日期:1996-07-01 00:00:00

  • A fast preparation of skeletal materials using enzyme maceration.

    abstract::The current study investigates the removal of soft tissues from mice and rats by the use of three different proteases and one lipase from Novozymes A/S. The results demonstrate the enzyme maceration to be remarkably fast (1-3 h) compared to the traditional warm-water procedure, which requires up to several days. In ad...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2010.01668.x

    authors: Simonsen KP,Rasmussen AR,Mathisen P,Petersen H,Borup F

    更新日期:2011-03-01 00:00:00

  • The utility of the proximal epiphysis of the fifth metatarsal in age estimation.

    abstract::Radiographs of 277 living individuals were assessed via a numerical scoring system to determine the timing of appearance and degree of fusion between the proximal epiphysis of the fifth metatarsal and its diaphysis. The epiphysis was observed to first appear in females at 8 years and 10 years in males and fuse by 14 y...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12069

    authors: Davies CM,Hackman L,Black S

    更新日期:2013-03-01 00:00:00

  • Revisiting the thermal development of latent fingerprints on porous surfaces: new aspects and refinements.

    abstract::Although the ability to develop latent fingerprints on paper using heat alone has been noted previously, it has been considered impractical for casework and inferior to other techniques. Here a new refinement of the technique is demonstrated for the high quality development of latent fingerprints on porous surfaces su...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2008.00902.x

    authors: Brown AG,Sommerville D,Reedy BJ,Shimmon RG,Tahtouh M

    更新日期:2009-01-01 00:00:00

  • Toolmark identification of a mattock to a clod of soil from a grave.

    abstract::At a recent bushland gravesite that contained a deceased male, a clod of soil displaying a striated toolmark was collected during the excavation of the grave. This clod was preserved, and the mark was cast using Mikrosil™. Shovels and a mattock, which had been discarded by the suspects, were subsequently found at anot...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2010.01537.x

    authors: Clark MD

    更新日期:2011-01-01 00:00:00

  • Forensic Gait Analysis and Recognition: Standards of Evidence Admissibility.

    abstract::Gait is one biological characteristic which has attracted strong research interest due to its potential use in human identification. Although almost two decades have passed since a forensic gait expert has testified to the identity of a perpetrator in court, the methods remain insufficiently robust, considering the re...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章,评审

    doi:10.1111/1556-4029.14036

    authors: Macoveciuc I,Rando CJ,Borrion H

    更新日期:2019-09-01 00:00:00

  • An evaluation of the reliability of Widmark calculations based on breath alcohol measurements.

    abstract::This study evaluated the reliability of Widmark calculations, based on breath ethanol reading (BrACs), for estimating the amount of alcohol consumed. A standard ethanol dose (males 0.51 g/kg; females 0.43 g/kg) was given to 115 college seniors, and BrACs were measured for two hours. Calculations of ethanol dose were p...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Friel PN,Logan BK,Baer J

    更新日期:1995-01-01 00:00:00

  • The assassination of the Swedish Minister for Foreign Affairs.

    abstract::On September 10, 2003, Anna Lindh, the Swedish Minister for Foreign Affairs, was assassinated. The offender, a 24-year-old man, was a socially isolated, culturally and familially dislocated, yet academically quite competent young man who became enthralled with the habitual criminality of some of his relatives and thei...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2010.01653.x

    authors: Unsgaard E,Meloy JR

    更新日期:2011-03-01 00:00:00

  • Interpreting the effects of burning on pre-incineration saw marks in bone.

    abstract::This study examined the effects of fire on the features associated with saw marks in bone. Both class and individual characteristics were examined using stereomicroscopy and scanning electron microscopy (SEM). Twenty-four semifleshed Sus scrofa L. tibiae were sawed into three sections with the middle section having de...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12580

    authors: Robbins SC,Fairgrieve SI,Oost TS

    更新日期:2015-01-01 00:00:00

  • Bacterial Translocation and Sample Contamination in Postmortem Microbiological Analyses.

    abstract::The diagnostic value of postmortem bacteriology has been discussed controversially for decades. In the study herein, contamination during sampling procedures and postmortem translocation were investigated to interpret postmortem microbiology results. One hundred medicolegal autopsy cases in total were included. Radiol...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12991

    authors: Palmiere C,Egger C,Prod'Hom G,Greub G

    更新日期:2016-03-01 00:00:00

  • Current practices by forensic anthropologists in adult skeletal age estimation.

    abstract::When determining an age estimate from adult skeletal remains, forensic anthropologists face a series of methodological choices. These decisions, such as which skeletal region to evaluate, which methods to apply, what statistical information to use, and how to combine information from multiple methods, ultimately impac...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2011.01979.x

    authors: Garvin HM,Passalacqua NV

    更新日期:2012-03-01 00:00:00

  • Decomposition of buried human bodies and associated death scene materials on coral atolls in the tropical Pacific.

    abstract::The decomposition of bodies and the decay of associated cultural materials, including clothing, personal ornaments, caskets, and grave goods, was investigated based on a series of exhumations on a small sand island on Kwajalein Atoll, Marshall Islands. Decay data were documented for an interment period between 40 and ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Spennemann DH,Franke B

    更新日期:1995-05-01 00:00:00

  • Correlation between saw blade width and kerf width.

    abstract::Most studies of saw marks have focused on morphological characteristics and their utility in identifying saws suspected to have been utilized in cases of criminal dismemberment. The present study examined the extent to which metric analysis may be used to correlate saw blade measurements with minimum kerf widths (MKWs...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.14556

    authors: Menschel M,Pokines JT,Reinecke G

    更新日期:2021-01-01 00:00:00

  • Measurements of ulna--a new method for determination of sex.

    abstract::Identification of sex from skeleton parts is of paramount importance to medicolegal investigations. The present study makes an attempt to assess sex from the ulna by devising new measurements for fragmentary bones. Dry and adult ulnae (100 male and 60 female) of Madhya Pradesh, India were subjected to three measuremen...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Purkait R

    更新日期:2001-07-01 00:00:00

  • Suicides by fall from height in Geneva, Switzerland, from 1991 to 2000.

    abstract::Suicides due to fall from height in Geneva from 1991 to 2000 were reviewed. Scene investigations, autopsy findings, psychiatric histories, and toxicology results were examined. There were 197 of these suicides, an incidence of five cases per 100,000 inhabitants per year or one fourth of all suicides in Geneva per year...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Perret G,Flomenbaum M,La Harpe R

    更新日期:2003-07-01 00:00:00

  • Adapting the Chumbley Score to Match Striae on Land Engraved Areas (LEAs) of Bullets.

    abstract::The same-source problem remains a major challenge in forensic toolmark and firearm examination. Here, we investigate the applicability of the Chumbley method (J Forensic Sci, 2018, 63, 849; J Forensic Sci, 2010, 55, 953) (10,12), developed for screwdriver markings, for same-source identification of striations on bulle...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.13950

    authors: Krishnan G,Hofmann H

    更新日期:2019-05-01 00:00:00

  • Distribution of allele frequencies for two tetranucleotide repeats (FES and D2S1328) among five Indian population groups.

    abstract:POPULATION:The distribution of allele frequencies have been analyzed at two short tandem repeat (STR) loci FES and D2S1328 among five anthropologically distinct ethnic groups of India namely Ezhavas, Nairs, Arayas, Vishwakarma and Muslims. Muslims are religio-ethnic group while other populations mentioned above belong ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Ahmad S,Seshadri M

    更新日期:2004-03-01 00:00:00

  • Revisiting arson from an outpatient forensic perspective.

    abstract::Progress in the understanding of individuals who commit arson has been on a slow but steady course over the past two decades. From our review of court-ordered outpatient forensic psychiatric evaluation of individuals charged with arson over a five-year period, preliminary prototypical profiles of the psychotic, mental...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Leong GB,Silva JA

    更新日期:1999-05-01 00:00:00

  • Postmortem distribution and redistribution of morphine in man.

    abstract::This study evaluated both site dependent differences and time dependent changes in postmortem morphine concentrations in man. In 32 deaths involving morphine, left ventricular blood, femoral blood, and cisternal cerebrospinal fluid, were collected as soon after death as possible (T1), and collected again together with...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Logan BK,Smirnow D

    更新日期:1996-03-01 00:00:00

  • Mastoid triangle for sex determination in adult Nigerian population: a validation study.

    abstract::In the quest for a simple, reliable technique to estimate the sex of human remains several novel metric skeletal indices have been reported. Only a few have been examined for utility in populations different from those in which they were developed. In this study, the mastoid process was evaluated for sex determination...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12299

    authors: Jaja BN,Ajua CO,Didia BC

    更新日期:2013-11-01 00:00:00

  • Increasing Allele Detection by Altering the Quantity of Internal Lane Standard.

    abstract::Electrokinetic injection (EI) is the primary method used in forensic laboratories to load amplified PCR product in capillary electrophoresis for short tandem repeat (STR) fragment separation. Because all samples subjected to capillary electrophoresis use internal lane standard (ILS), this study investigated the conseq...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12945

    authors: Esparza JM,Michalik M,Dukes MJ,Wojtkiewicz P

    更新日期:2016-01-01 00:00:00

  • Suicidal asphyxiation by inhalation of automobile emission without carbon monoxide poisoning.

    abstract::Reported herein is the suicidal asphyxiation of a young man due to exhaustion of oxygen in the interior of a sealed automobile into which the exhaust emissions were diverted. His blood carboxyhemaglobin concentration was less than 5% saturation. The car was equipped with a catalytic converter and when tested, the exha...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2006.00224.x

    authors: deRoux SJ

    更新日期:2006-09-01 00:00:00

  • Incidental cardiac rhabdomyomas: a significant finding necessitating additional investigation at the time of autopsy.

    abstract::Cardiac rhabdomyomas are rare lesions forming part of the tuberous sclerosis complex that may be responsible for sudden death. As well as remaining clinically occult for variable periods of time, they may, along with other manifestations of tuberous sclerosis, be quite difficult to detect clinically and pathologically...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Byard RW,Smith NM,Bourne AJ

    更新日期:1991-07-01 00:00:00

  • Utility of the frontonasal suture for estimating age at death in human skeletal remains.

    abstract::This project evaluated the utility of the frontonasal suture for estimating age at death. Utilizing human remains of known age at death with varying degrees of fusion, curated at the American Museum of Natural History in New York City and the Smithsonian Institution's National Museum of Natural History in Washington, ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2012.02193.x

    authors: Alesbury HS,Ubelaker DH,Bernstein R

    更新日期:2013-01-01 00:00:00