Abstract:
:Vibrio vulnificus, an inhabitant of marine and estuarine environments around the world, is the leading cause of reported seafood-related deaths in the United States. Disease is caused by opaque colony-forming strains that produce capsular polysaccharide, loss of which results in an unencapsulated translucent phenotype with diminished virulence potential. Rugose is a third phenotypic variant of V. vulnificus, and produces a separate exopolysaccharide that results in a dry, wrinkled appearance and the ability to form profuse biofilms. Phase variation among these three phenotypes is influenced by several environmental factors, including the presence of calcium in the medium (Garrison-Schilling et al.). In this study, we have identified a second cation, manganese, which substantially increases the propensity of opaque V. vulnificus strains to switch to translucent or rugose phenotypes. In comparative studies, manganese and calcium promoted switching to the same phenotype for some strains but to different phenotypes for others, results of which indicate that the two cations do not always promote the same changes in underlying gene expression. The data here provide further evidence that exposure of V. vulnificus to select cations results in phenotypic changes that impact both virulence capacity and ecology of the organism.
journal_name
Environ Microbiol Repjournal_title
Environmental microbiology reportsauthors
Kaluskar ZM,Garrison-Schilling KL,McCarter KS,Lambert B,Simar SR,Pettis GSdoi
10.1111/1758-2229.12318subject
Has Abstractpub_date
2015-10-01 00:00:00pages
789-94issue
5issn
1758-2229journal_volume
7pub_type
杂志文章abstract::Antibiotic resistance is a rising threat for human health. Although in clinical settings and terrestrial environments the rise of antibiotic resistant bacteria is well documented, their dissemination and spread in the marine environment, covering almost two-thirds of the Earth's surface, is still poorly understood. In...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12665
更新日期:2018-08-01 00:00:00
abstract::Bacteria-host interactions are universal in nature and have significant effects on host functionality. Bacterial secondary metabolites are believed to play key roles in such interactions as well as in interactions within the host-associated microbial community. Hence, prominent secondary metabolite-producing bacteria ...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12698
更新日期:2019-06-01 00:00:00
abstract::ITS primers commonly used to describe soil fungi are flawed for AMF although it is unknown the extent to which they distort the interpretation of community patterns. Here, we focus on how the use of a specific ITS2 fungal barcoding primer pair biased for AMF changes the interpretation of AMF community patterns from th...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12574
更新日期:2017-10-01 00:00:00
abstract::The extent to which different sequence-based approaches describe environmental microbial communities in comparative studies is an important consideration when deriving inferences from ecological studies. The ability of a targeted metagenomic approach [small subunit (SSU) rRNA pyrosequencing] and shotgun metagenome app...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2012.00328.x
更新日期:2012-04-01 00:00:00
abstract::The shallow submarine hydrothermal field of the Prony Bay (New Caledonia) discharges hydrogen- and methane-rich fluids with low salinity, temperature (< 40°C) and high pH (11) produced by the serpentinization reactions of the ultramafic basement into the lagoon seawater. They are responsible for the formation of carbo...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12184
更新日期:2014-12-01 00:00:00
abstract::The TM6 phylum belongs to the so-called microbial dark matter that gathers uncultivated bacteria detected only via DNA sequencing. Recently, the genome sequence of a TM6 bacterium (TM6SC1) has led to suggest that this bacterium would adopt an endosymbiotic life. In the present paper, free-living amoebae bearing a TM6 ...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12343
更新日期:2015-12-01 00:00:00
abstract::Several bacterial strains of the Pseudomonas genus provide plant growth stimulation, plant protection against pests or bioremediation. Among these bacteria, P. fluorescens Pf29Arp reduces the severity of take-all, a disease caused by the pathogenic fungus Gaeumannomyces graminis var. tritici (Ggt) on wheat roots. In t...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12048
更新日期:2013-06-01 00:00:00
abstract::Ectosymbiotic Nanoarchaeota live on the surface of diverse archaeal hosts. Despite being broadly distributed in global geothermal systems, only three Nanoarchaeota have been successfully co-cultivated with their hosts, and until now no nanoarchaeotal cultures or genomes have been described from deep-sea hydrothermal v...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12740
更新日期:2019-04-01 00:00:00
abstract::Pyrrolysine (Pyl), the 22nd canonical amino acid, is only decoded and synthesized by a limited number of organisms in the domains Archaea and Bacteria. Pyl is encoded by the amber codon UAG, typically a stop codon. To date, all known Pyl-decoding archaea are able to carry out methylotrophic methanogenesis. The functio...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12545
更新日期:2017-08-01 00:00:00
abstract::Free-living bacteria such as Pseudomonas putida are frequently exposed to temperature shifts and non-optimal growth conditions. We compared the transcriptome and metabolome of the cold adaptation of P. putida KT2440 and isogenic cold-sensitive transposon mutants carrying transposons in their cbrA, cbrB, pcnB, vacB, an...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12793
更新日期:2019-12-01 00:00:00
abstract::The particulate methane monooxygenase (pMMO) in Methylocystis strain SB2 was found to be constitutively expressed in the absence of methane when the strain was grown on either acetate or ethanol. Real-time quantitative polymerase chain reaction (PCR) and reverse transcription-PCR showed that the expression of pmoA dec...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2010.00205.x
更新日期:2011-04-01 00:00:00
abstract::Pseudomonas putida KF715 exhibits unique properties in both catabolic activity and genome plasticity. Our previous studies revealed that the DNA region containing biphenyl and salycilate metabolism gene clusters (termed the bph-sal element) was frequently deleted and transferred by conjugation to closely related P. pu...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12561
更新日期:2017-10-01 00:00:00
abstract::The bacterial adenylate cyclase two-hybrid (BACTH) system has been widely used to characterize protein-protein interactions in the prokaryotic world. This system relies on the interaction-mediated reconstitution of adenylate cyclase activity in Escherichia coli by bringing together two complementary fragments of the c...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12123
更新日期:2014-06-01 00:00:00
abstract::Previous studies examining the clonal diversity of Escherichia coli populations within humans have been based on faecal isolates. In this study E. coli were isolated from biopsies taken from the terminal ileum, ascending, transverse and descending colon, and rectum of 69 individuals. Multiple isolates from each biopsy...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12300
更新日期:2015-08-01 00:00:00
abstract::Tropospheric nitrate levels are predicted to increase throughout the 21st century, with potential effects on terrestrial ecosystems, including the Greenland ice sheet (GrIS). This study considers the impacts of elevated nitrate concentrations on the abundance and composition of dominant bulk and active prokaryotic com...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12510
更新日期:2017-04-01 00:00:00
abstract::Bacteria can manipulate their surrounding environment through the secretion of proteins into other living organisms and into the extracellular milieu. In Gram stain negative bacteria this process is mediated by different types of secretion systems from type I through type VI secretion system (T1SS-T6SS). In this study...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2012.00394.x
更新日期:2013-02-01 00:00:00
abstract::Although cyanobacterial diazotrophs are common in Arctic terrestrial and freshwater habitats, they have been assumed to be absent from Arctic marine habitats. We report here a high diversity of cyanobacterial nifH genes in Fram Strait and the Greenland Sea. The nifH gene encodes the iron protein of the nitrogenase enz...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2012.00343.x
更新日期:2012-06-01 00:00:00
abstract::Identification of transmission routes and of factors affecting the spatial positions of pathogens, hosts and vectors is basic to an adequate disease management. Nosema ceranae is a Microsporidian recently described as a parasite of Apis mellifera honeybees and is currently considered the aetiological agent of an emerg...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2010.00186.x
更新日期:2011-02-01 00:00:00
abstract::Dual stable isotope probing has been used to infer rates of microbial biomass production and modes of carbon fixation. In order to validate this approach for assessing archaeal production, the methanogenic archaeon Methanosarcina barkeri was grown either with H2 , acetate or methanol with D2 O and 13 C-dissolved inorg...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12876
更新日期:2020-10-01 00:00:00
abstract::Given the key role played by honey bees in almost all terrestrial ecosystems, maintaining bee populations in adequate sanitary conditions is crucial for these essential pollinators to continue their work. From the beginning of the 21st century, beekeepers have reported a progressive increase in the overwintering morta...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2011.00299.x
更新日期:2012-02-01 00:00:00
abstract::Lack of the Pseudomonas putida PP2258 protein or its overexpression results in defective motility on solid media. The PP2258 protein is tripartite, possessing a PAS domain linked to two domains associated with turnover of c-di-GMP - a cyclic nucleotide that controls the switch between motile and sessile lifestyles. Th...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12045
更新日期:2013-08-01 00:00:00
abstract::In anoxic environments such as swamps, rice fields and sludge digestors, syntrophic microbial communities are important for decomposition of organic matter to CO2 and CH4 . The most difficult step is the fermentative degradation of short-chain fatty acids such as propionate and butyrate. Conversion of these metabolite...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2010.00147.x
更新日期:2010-08-01 00:00:00
abstract::In complex environments such as cheeses, the lack of relevant information on the physiology and virulence expression of pathogenic bacteria and the impact of endogenous microbiota has hindered progress in risk assessment and control. Here, we investigated the behaviour of Staphylococcus aureus, a major foodborne patho...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2010.00230.x
更新日期:2011-06-01 00:00:00
abstract::Pseudomonas aeruginosa produces the toxic secondary metabolite hydrogen cyanide (HCN) at high cell population densities and low aeration. Here, we investigated the impact of HCN as a signal in cell-cell communication by comparing the transcriptome of the wild-type strain PAO1 to that of an HCN-negative mutant under cy...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12105
更新日期:2014-02-01 00:00:00
abstract::Haplodiploidy is a sex determination system in which fertilized diploid eggs develop into females and unfertilized haploid eggs develop into males. The evolutionary explanations for this phenomenon include the possibility that haplodiploidy can be reinforced by infection with endosymbiotic bacteria, such as Wolbachia....
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12425
更新日期:2016-10-01 00:00:00
abstract::Different oceanographic provinces host discrete microbial assemblages that are adapted to local physicochemical conditions. We sequenced and compared the metagenomes of two microbial communities inhabiting adjacent water masses in the Tasman Sea, where the recent strengthening of the East Australian Current (EAC) has ...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2012.00362.x
更新日期:2012-10-01 00:00:00
abstract::In this study, an aquaporin protein, Aqp1, in Cryptococcus neoformans, which can lead either saprobic or parasitic lifestyles and causes life-threatening fungal meningitis was identified and characterized. AQP1 expression was rapidly induced (via the HOG pathway) by osmotic or oxidative stress. In spite of such transc...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12527
更新日期:2017-06-01 00:00:00
abstract::The increase in the areal extent of the subtropical gyres over the past decade has been attributed to a global tendency towards increased water-column stratification. Here, we examine how vertical stratification governs the community structure of the picophytoplankton that dominate these vast marine ecosystems. We ana...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2011.00241.x
更新日期:2011-08-01 00:00:00
abstract::Electrodes poised at potentials low enough to serve as an electron donor for microbial respiration, but high enough to avoid the production of hydrogen, have been proposed as an alternative to the use of soluble electron donors for stimulating the bioremediation of chlorinated contaminants and/or metals. However, this...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2009.00118.x
更新日期:2010-04-01 00:00:00
abstract::In this study, we report the presence of the El Tor CTXΦ and classical CTXΦ in Vibrio cholerae O1 strains isolated from Varanasi, India. Polymerase chain reaction, DNA sequencing and restriction fragment length polymorphism revealed that, although ctx-positive strains isolated after 1990 contain CTXΦ harbouring El Tor...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2011.00287.x
更新日期:2011-12-01 00:00:00