Hydrodynamic collective effects of active protein machines in solution and lipid bilayers.

abstract：:Ribonucleotide reductase provides deoxynucleotides for nuclear and mitochondrial (mt) DNA replication and repair. The mammalian enzyme consists of a catalytic (R1) and a radical-generating (R2 or p53R2) subunit. During S-phase, a R1/R2 complex is the major provider of deoxynucleotides. p53R2 is induced by p53 after DN...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pontarin G,Fijolek A,Pizzo P,Ferraro P,Rampazzo C,Pozzan T,Thelander L,Reichard PA,Bianchi V

更新日期：2008-11-18 00:00:00

abstract：:Neutrophils and dendritic cells when migrating in confined environments have been shown to actuate a directional choice toward paths of least hydraulic resistance (barotaxis), in some cases overriding chemotactic responses. Here, we investigate whether this barotactic response is conserved in the more primitive model ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Belotti Y,McGloin D,Weijer CJ

更新日期：2020-10-13 00:00:00

abstract：:An open question remains as to what coordinates cell behavior during organogenesis, permitting organs to reach their appropriate size and shape. The Arabidopsis gene STRUBBELIG (SUB) defines a receptor-mediated signaling pathway in plants. SUB encodes a putative leucine-rich repeat transmembrane receptor-like kinase. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chevalier D,Batoux M,Fulton L,Pfister K,Yadav RK,Schellenberg M,Schneitz K

更新日期：2005-06-21 00:00:00

abstract：:The values of the moment of inertia of the ground-state band of (168)Hf from J = 0(+) to 22(+) are interpreted as involving a revolving cluster with composition increasing steadily from p(10.5)n(14) to p(20.5)n(24), with radius of revolution 6.390 fm. The excited bands from 14(+) to 34(+), 21(-) to 33(-), and 18(-) to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pauling L

更新日期：1984-05-01 00:00:00

abstract：:Duplication of the genome during the S phase of the cell cycle does not occur simultaneously; rather, different sequences are replicated at different times. The replication timing of specific sequences can change during development; however, the determinants of this dynamic process are poorly understood. To gain insig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： White EJ,Emanuelsson O,Scalzo D,Royce T,Kosak S,Oakeley EJ,Weissman S,Gerstein M,Groudine M,Snyder M,Schübeler D

更新日期：2004-12-21 00:00:00

abstract：:The three lipin phosphatidate phosphatase (PAP) enzymes catalyze a step in glycerolipid biosynthesis, the conversion of phosphatidate to diacylglycerol. Lipin-1 is critical for lipid synthesis and homeostasis in adipose tissue, liver, muscle, and peripheral nerves. Little is known about the physiological role of lipin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dwyer JR,Donkor J,Zhang P,Csaki LS,Vergnes L,Lee JM,Dewald J,Brindley DN,Atti E,Tetradis S,Yoshinaga Y,De Jong PJ,Fong LG,Young SG,Reue K

更新日期：2012-09-11 00:00:00

abstract：:Malaria, caused by Plasmodium sp, results in almost one million deaths and over 200 million new infections annually. The World Health Organization has recommended that artemisinin-based combination therapies be used for treatment of malaria. Artemisinin is a sesquiterpene lactone isolated from the plant Artemisia annu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Westfall PJ,Pitera DJ,Lenihan JR,Eng D,Woolard FX,Regentin R,Horning T,Tsuruta H,Melis DJ,Owens A,Fickes S,Diola D,Benjamin KR,Keasling JD,Leavell MD,McPhee DJ,Renninger NS,Newman JD,Paddon CJ

更新日期：2012-01-17 00:00:00

abstract：:As part of a study into the molecular basis of attenuation and reversion to neurovirulence in the Sabin poliovirus vaccines, we have determined the complete nucleotide sequence of a cloned DNA copy of the genome of P3/Leon/37, the neurovirulent progenitor of the type 3 Sabin vaccine strain, P3/Leon 12a1b. Comparison o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stanway G,Hughes PJ,Mountford RC,Reeve P,Minor PD,Schild GC,Almond JW

更新日期：1984-03-01 00:00:00

abstract：:Almost all democratic societies evolved socially and politically out of authoritarian and nondemocratic regimes. These changes not only altered the allocation of economic resources in society but also the structure of political power. In this paper, we develop a framework for studying the dynamics of political and soc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Acemoglu D,Egorov G,Sonin K

更新日期：2011-12-27 00:00:00

abstract：:The technique of self-sustained sequence replication allows isothermal amplification of DNA and RNA molecules in vitro. This method relies on the activities of a reverse transcriptase and a DNA-dependent RNA polymerase to amplify specific nucleic acid sequences. We have modified this protocol to allow selective amplif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Breaker RR,Joyce GF

更新日期：1994-06-21 00:00:00

abstract：:Regeneration of appendages is frequent among invertebrates as well as some vertebrates. However, in mammals this has been largely relegated to digit tip regeneration, as found in mice and humans. The regenerated structures are formed from a mound of undifferentiated cells called a blastema, found just below the site o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehoczky JA,Robert B,Tabin CJ

更新日期：2011-12-20 00:00:00

abstract：:An ability to safely harness the powerful regenerative potential of adult stem cells for clinical applications is critically dependent on a comprehensive understanding of the underlying mechanisms regulating their activity. Epithelial organoid cultures accurately recapitulate many features of in vivo stem cell-driven ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murakami K,Terakado Y,Saito K,Jomen Y,Takeda H,Oshima M,Barker N

更新日期：2021-01-26 00:00:00

abstract：:Accumulative aggregation of mutant Huntingtin (Htt) is a primary neuropathological hallmark of Huntington's disease (HD). Currently, mechanistic understanding of the cytotoxicity of mutant Htt aggregates remains limited, and neuroprotective strategies combating mutant Htt-induced neurodegeneration are lacking. Here, w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu Y,Li C,Tao X,Brazill JM,Park J,Diaz-Perez Z,Zhai RG

更新日期：2019-09-17 00:00:00

abstract：:The WWOX gene is a recently cloned tumor suppressor gene that spans the FRA16D fragile region. Wwox protein contains two WW domains that are generally known to mediate protein-protein interaction. Here we show that Wwox physically interacts via its first WW domain with the p53 homolog, p73. The tyrosine kinase, Src, p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aqeilan RI,Pekarsky Y,Herrero JJ,Palamarchuk A,Letofsky J,Druck T,Trapasso F,Han SY,Melino G,Huebner K,Croce CM

更新日期：2004-03-30 00:00:00

abstract：:The difficulty of achieving robust functional expression of insect nicotinic acetylcholine receptors (nAChRs) has hampered our understanding of these important molecular targets of globally deployed neonicotinoid insecticides at a time when concerns have grown regarding the toxicity of this chemotype to insect pollina...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ihara M,Furutani S,Shigetou S,Shimada S,Niki K,Komori Y,Kamiya M,Koizumi W,Magara L,Hikida M,Noguchi A,Okuhara D,Yoshinari Y,Kondo S,Tanimoto H,Niwa R,Sattelle DB,Matsuda K

更新日期：2020-07-14 00:00:00

abstract：:Assembly of nitrogenase FeMoco is one of the key processes in bioinorganic chemistry. NifB and NifEN are two essential elements immediately adjacent to each other along the biosynthetic pathway of FeMoco. Previously, an 8Fe-precursor of FeMoco was identified on NifEN; however, the identity of the biosynthetic intermed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wiig JA,Hu Y,Ribbe MW

更新日期：2011-05-24 00:00:00

abstract：:Malaria infection triggers vigorous host immune responses; however, the parasite ligands, host receptors, and the signaling pathways responsible for these reactions remain unknown or controversial. Malaria parasites primarily reside within RBCs, thereby hiding themselves from direct contact and recognition by host imm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu J,Tian L,Yu X,Pattaradilokrat S,Li J,Wang M,Yu W,Qi Y,Zeituni AE,Nair SC,Crampton SP,Orandle MS,Bolland SM,Qi CF,Long CA,Myers TG,Coligan JE,Wang R,Su XZ

更新日期：2014-01-28 00:00:00

abstract：:Studies to elucidate the mode of action of aldosterone have been carried out in the amphibian urinary bladder. The following previously reported hypotheses were evaluated: (1) aldosterone stimulates sodium transport by increasing the amount of sodium available to the sodium pump; (2) aldosterone enhances energy produc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goodman DB,Allen JE,Rasmussen H

更新日期：1969-09-01 00:00:00

abstract：:A major challenge in research on executive control is to reveal its functional decomposition into underlying neural mechanisms. A typical assumption is that this decomposition occurs solely through anatomically based dissociations. Here we tested an alternative hypothesis that different cognitive control processes may...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Braver TS,Paxton JL,Locke HS,Barch DM

更新日期：2009-05-05 00:00:00

abstract：:Prostate cancer is a leading cause of cancer death in men over 50 years of age, and there is a characteristic marked decrease in Zn content in the malignant prostate cells. The cause and consequences of this loss have thus far been unknown. We found that in middle-aged rats a Zn-deficient diet reduces prostatic Zn lev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fong LY,Jing R,Smalley KJ,Wang ZX,Taccioli C,Fan S,Chen H,Alder H,Huebner K,Farber JL,Fiehn O,Croce CM

更新日期：2018-11-20 00:00:00

abstract：:Visual information is conveyed from the eye to the brain by distinct types of retinal ganglion cells (RGCs). It is largely unknown how RGCs acquire their defining morphological and physiological features and connect to upstream and downstream synaptic partners. The three Brn3/Pou4f transcription factors (TFs) particip...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sajgo S,Ghinia MG,Brooks M,Kretschmer F,Chuang K,Hiriyanna S,Wu Z,Popescu O,Badea TC

更新日期：2017-05-16 00:00:00

abstract：:Memory CD8 T cells provide durable protection against diverse intracellular pathogens and can be broadly segregated into distinct circulating and tissue-resident populations. Paradigmatic studies have demonstrated that circulating memory cells can be further divided into effector memory (Tem) and central memory (Tcm) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Milner JJ,Nguyen H,Omilusik K,Reina-Campos M,Tsai M,Toma C,Delpoux A,Boland BS,Hedrick SM,Chang JT,Goldrath AW

更新日期：2020-10-13 00:00:00

abstract：:We stabilize nematic droplets with handles against surface tension-driven instabilities, using a yield-stress material as outer fluid, and study the complex nematic textures and defect structures that result from the competition between topological constraints and the elasticity of the nematic liquid crystal. We uncov...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pairam E,Vallamkondu J,Koning V,van Zuiden BC,Ellis PW,Bates MA,Vitelli V,Fernandez-Nieves A

更新日期：2013-06-04 00:00:00

abstract：:Transcripts from mitochondrial (maxicircle) genes of kinetoplastid organisms undergo RNA editing characterized by a series of reactions that insert and delete uridine nucleotides within the sequence of the pre-mRNAs. Guide RNAs, which complement fully edited mRNAs, provide the information for the edited sequence by an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Göringer HU,Koslowsky DJ,Morales TH,Stuart K

更新日期：1994-03-01 00:00:00

abstract：:Optogenetic methods have been highly effective for suppressing neural activity and modulating behavior in rodents, but effects have been much smaller in primates, which have much larger brains. Here, we present a suite of technologies to use optogenetics effectively in primates and apply these tools to a classic quest...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Acker L,Pino EN,Boyden ES,Desimone R

更新日期：2016-11-15 00:00:00

abstract：:The rpoS gene of Escherichia coli encodes a putative RNA polymerase sigma factor that is considered to be the central regulator of gene expression in stationary phase. The gene product (sigma 38) was overproduced using the cloned rpoS gene and purified to homogeneity. Reconstituted RNA polymerase holoenzyme (E sigma 3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tanaka K,Takayanagi Y,Fujita N,Ishihama A,Takahashi H

更新日期：1993-04-15 00:00:00

abstract：:Efforts are underway to construct several recoded genomes anticipated to exhibit multivirus resistance, enhanced nonstandard amino acid (nsAA) incorporation, and capability for synthetic biocontainment. Although our laboratory pioneered the first genomically recoded organism (Escherichia coli strain C321.∆A), its fitn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wannier TM,Kunjapur AM,Rice DP,McDonald MJ,Desai MM,Church GM

更新日期：2018-03-20 00:00:00

abstract：:Diphosphoinositol pentakisphosphate (PP-IP5) and bis(diphospho)inositol tetrakisphosphate (bis-PP-IP4) are recently identified inositol phosphates that possess pyrophosphate bonds. We have purified an inositol hexakisphosphate (IP6) kinase from rat brain supernatants. The pure protein, a monomer of 54 kDa, displays hi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Voglmaier SM,Bembenek ME,Kaplin AI,Dormán G,Olszewski JD,Prestwich GD,Snyder SH

更新日期：1996-04-30 00:00:00

abstract：:Fifty cats with feline leukemia virus (FeLV) infection and leukemia-lymphoma complex were treated by ex vivo immunoadsorption with Staphylococcus protein A-bound filters. Most cats responded to therapy. Twelve showed tumor regression, including disappearance of tumor cells, but died later of other complications. Three...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu WT,Engelman RW,Trang LQ,Hau K,Good RA,Day NK

更新日期：1984-06-01 00:00:00

abstract：:The tumor suppressor protein p53 localizes to microtubules (MT) and, in response to DNA damage, is transported to the nucleus via the MT minus-end-directed motor protein dynein. Dynein is also responsible for MT-mediated nuclear targeting of adenovirus type 2 (Ad2). Here we show that treatment with low concentrations ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giannakakou P,Nakano M,Nicolaou KC,O'Brate A,Yu J,Blagosklonny MV,Greber UF,Fojo T

更新日期：2002-08-06 00:00:00