Abstract:
AIM:In nondividing neurons examine the role of Gadd45b in active 5-methylcytosine (5MC) and 5-hydroxymethylcytosine (5HMC) removal at a gene promoter highly implicated in mental illnesses and cognition, Bdnf. MATERIALS & METHODS:Mouse primary cortical neuronal cultures with and without Gadd45b siRNA transfection were treated with N-methyl-d-aspartate (NMDA). Expression changes of genes reportedly involved in DNA demethylation, Bdnf mRNA and protein and 5MC and 5HMC at Bdnf promoters were measured. RESULTS:Gadd45b siRNA transfection in neurons abolishes the NMDA-induced increase in Bdnf IXa mRNA and reductions in 5MC and 5HMC at the Bdnf IXa promoter. CONCLUSION:These results contribute to our understanding of DNA demethylation mechanisms in neurons, and its role in regulating NMDA responsive genes implicated in mental illnesses.
journal_name
Epigenomicsjournal_title
Epigenomicsauthors
Gavin DP,Kusumo H,Sharma RP,Guizzetti M,Guidotti A,Pandey SCdoi
10.2217/epi.15.12subject
Has Abstractpub_date
2015-01-01 00:00:00pages
567-79issue
4eissn
1750-1911issn
1750-192Xjournal_volume
7pub_type
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