Abstract:
:microRNAs are an abundant class of small non-coding RNAs that control gene expression post-transcriptionally. Importantly, microRNA activity participates in the regulation of cellular processes and is a potentially valuable source of biomarkers in the diagnosis and prognosis of human diseases. Here we introduce miQPCR, an innovative method to quantify microRNAs expression by using Real-Time PCR. miQPCR exploits T4 RNA ligase activities to extend uniformly microRNAs' 3'-ends by addition of a linker-adapter. The adapter is then used as 'anchor' to prime cDNA synthesis and throughout qPCR to amplify specifically target amplicons. miQPCR is an open, adaptable and cost-effective procedure, which offers the following advantages; i) universal elongation and reverse transcription of all microRNAs; ii) Tm-adjustment of microRNA-specific primers; iii) high sensitivity and specificity in discriminating among closely related sequences and; iv) suitable for the analysis of cellular and cell-free circulating microRNAs. Analysis of cellular and cell-free circulating microRNAs secreted by rat primary hepatocytes stimulated with cytokines and growth factors identifies for the first time a widespread modulation of both microRNAs expression and secretion. Altogether, our findings suggest that the pleiotropic activity of humoral factors on microRNAs may extensively affect liver function in response to injury and regeneration.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Benes V,Collier P,Kordes C,Stolte J,Rausch T,Muckentaler MU,Häussinger D,Castoldi Mdoi
10.1038/srep11590subject
Has Abstractpub_date
2015-06-25 00:00:00pages
11590issn
2045-2322pii
srep11590journal_volume
5pub_type
杂志文章abstract::Programmed death-1 (PD-1) is an immunosuppressive receptor functionally bound with programmed death-ligand 1 (PD-L1), which has been reported in various malignancies. However, only a few studies are available for the clinical significance of PD-1/PD-L1 in nasopharyngeal carcinoma (NPC). In this study, we aim to invest...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43627
更新日期:2017-03-03 00:00:00
abstract::Pulsatile motion occurs in the cardiac systolic period and leads to significantly larger displacement of water molecules as it is observed during diffusion weighted image acquisition. Obvious pulsatile motion arises in the brain stem and basal ganglia and might affect the corticospinal tract. So far there is no consen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29525-0
更新日期:2018-07-25 00:00:00
abstract::Modulation of photoluminescence of atomically thin transition metal dichalcogenide two-dimensional materials is critical for their integration in optoelectronic and photonic device applications. By coupling with different plasmonic array geometries, we have shown that the photoluminescence intensity can be enhanced an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41175
更新日期:2017-01-30 00:00:00
abstract::This study aimed to determine the clinicopathological features of the subtypes of ampullary carcinoma (AC) to explore the indications for endoscopic papillectomy (EP) in early AC. Fifty-seven patients with AC who underwent curative resection were retrospectively reviewed. The 0/IA stages were significantly more common...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79836-4
更新日期:2021-01-12 00:00:00
abstract::Although graphene is a typical two dimensional materials, it has converted to multi-dimensional materials with many unique properties. As an example, the one dimensional graphene fiber is fabricated by utilizing ionic liquid as coagulation and functional diamines as cross-linkers to connect graphene oxide layers. The ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29534
更新日期:2016-07-08 00:00:00
abstract::Cartilage loss is a leading cause of disability among adults and effective therapy remains elusive. Neonatal chondrocytes (NChons) are an attractive allogeneic cell source for cartilage repair, but their clinical translation has been hindered by scarce donor availability. Here we examine the potential for catalyzing c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03553
更新日期:2013-12-19 00:00:00
abstract::We investigate whether the collapse of the quantum state of a single photon split between two space-like separated places takes a nonvanishing time. We realize this by using a source of heralded single photons, then splitting the resulting single photon state and letting it propagate over distances much larger than th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48387-8
更新日期:2019-08-15 00:00:00
abstract::Coastal zones constitute one of the most heavily populated and developed land zones in the world. Despite the utility and economic benefits that coasts provide, there is no reliable global-scale assessment of historical shoreline change trends. Here, via the use of freely available optical satellite images captured si...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24630-6
更新日期:2018-04-27 00:00:00
abstract::Little is known of how fungal distribution ranges vary with elevation. We studied fungal diversity and community composition from 740 to 2940 m above sea level on Mt. Norikura, Japan, sequencing the ITS2 region. There was a clear trend, repeated across each of the fungal phyla (Basidiomycota, Ascomycota, Zygomycota, C...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43025-9
更新日期:2019-04-25 00:00:00
abstract::Each year, droughts cause significant economic and agricultural losses across the world. The early warning and onset detection of drought is of particular importance for effective agriculture and water resource management. Previous studies show that the Standard Precipitation Index (SPI), a measure of precipitation de...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08553
更新日期:2015-02-25 00:00:00
abstract::Field electron emission (FEE) properties of vertically aligned hexagonal boron nitride nanowalls (hBNNWs) grown on Si have been markedly enhanced through the use of nitrogen doped nanocrystalline diamond (nNCD) films as an interlayer. The FEE properties of hBNNWs-nNCD heterostructures show a low turn-on field of 15.2 ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29444
更新日期:2016-07-11 00:00:00
abstract::Gene loss is a major contributor to the evolution of bacterial gene content. Gene loss may occur as a result of shifts in environment leading to changes in the intensity and/or directionality of selection applied for the maintenance of specific genes. Gene loss may also occur in a more neutral manner, when gene functi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35168
更新日期:2016-10-13 00:00:00
abstract::HilD is an AraC-like transcriptional regulator encoded in the Salmonella pathogenicity island 1 (SPI-1), which actives transcription of many genes within and outside SPI-1 that are mainly required for invasion of Salmonella into host cells. HilD controls expression of target genes directly or by acting through distinc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49192-z
更新日期:2019-09-04 00:00:00
abstract::The fungal communities of different soil compartments in mangrove ecosystem are poorly studied. We sequenced the internal transcribed spacer (ITS) regions to characterize the fungal communities in Avicennia marina root-associated soils (rhizosphere and pneumatophore) and bulk soil compartments. The rhizosphere but not...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09281-3
更新日期:2017-08-17 00:00:00
abstract::Phosphorus (P) is a non-renewable resource which may be depleted within next few decades; hence high P use efficiency is need of time. Plants have evolved an array of adaptive mechanisms to enhance external P acquisition and reprioritize internal utilization under P deficiency. Tissue specific biomass and P allocation...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61147-3
更新日期:2020-03-09 00:00:00
abstract::Electron ptychography has seen a recent surge of interest for phase sensitive imaging at atomic or near-atomic resolution. However, applications are so far mainly limited to radiation-hard samples, because the required doses are too high for imaging biological samples at high resolution. We propose the use of non-conv...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07488-y
更新日期:2017-08-29 00:00:00
abstract::We propose and demonstrate a type of a broadband half-wave plate that operates in the reflective mode. It consists of a metal grating embedded in a dielectric slab and placed on top of a grounded metal surface. We theoretically show that owing to the optical feedback effect which originates from the wave reflections a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09561-y
更新日期:2017-08-22 00:00:00
abstract::We present the synthesis of new composite materials based on copper nanoparticles (Cu NPs) deposited onto montmorillonite (MK10) and quartz sand, for degradation of atrazine, in the context of an advanced oxidation process (AOP). The synthesis involves a first step in which polyethylenimine (PEI) capped Cu NPs (PEI_Cu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01429-5
更新日期:2017-05-03 00:00:00
abstract::Fracture of initially crack-free bodies often occurs due to plastic instabilities known as shear bands. Previous computer simulations advanced a myriad of mechanisms to rationalize shear banding. However, they were restricted to planar geometries. We investigate the relevance of anisotropic plasticity by picking an ax...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-38437-y
更新日期:2019-02-05 00:00:00
abstract::Regular overconsumption of sugar is associated with obesity and type-2 diabetes, but how genetic factors contribute to variable sugar preferences and intake levels remains mostly unclear. Here we provide evidence for the usefulness of a Drosophila larva model to investigate genetic influence on vulnerability to sugar ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17760-w
更新日期:2017-12-15 00:00:00
abstract::We have proposed a scheme and presented the first experimental demonstration of acoustic illusion, by using anisotropic metamaterials to manipulate the acoustic field near boundaries of arbitrary curved geometry. Numerical simulations and experimental results show that in the presence of an illusion cloak, any object ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01427
更新日期:2013-01-01 00:00:00
abstract::The synthesis of highly biocompatible polymers is important for modern biotechnologies and medicine. Here, we report a unique process based on a two-step high-pressure ramp (HPR) for the ultrafast and efficient bulk polymerization of 2-(hydroxyethyl)methacrylate (HEMA) at room temperature without photo- and thermal ac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18244
更新日期:2015-12-16 00:00:00
abstract::Plant species of the genus Cecropia (Urticaceae) are used as traditional medicine in Latin-America, and are commercially available as food supplements. The aim of this study was to characterize and compare the phytochemical constituents of four Cecropia species collected in Panama. The structures of 11 compounds isola...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-38334-4
更新日期:2019-02-11 00:00:00
abstract::The structural and mechanical properties of tissue and the interplay between them play a critical role in tissue function. We introduce the optomechanogram, a combined quantitative and qualitative visualization of spatially co-registered measurements of the microstructural and micromechanical properties of any tissue....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79602-6
更新日期:2021-01-11 00:00:00
abstract::The colon can have an absorptive function similar to that of the small intestine after the massive resection of the small bowel. To improve colonic absorptive function, we created a valve in the colon (artificial colonic valve, ACV). ACVs were created in 20 rats that had 80 percent of their small intestine resected, w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-57865-3
更新日期:2020-01-21 00:00:00
abstract::Bacterial environmental and surgical site infection (SSI) surveillance was implemented from 2011-2016 in a UK Equine Referral Veterinary Hospital and identified 81 methicillin-resistant Staphylococcus aureus (MRSA) isolates. A cluster of MRSA SSIs occurred in early 2016 with the isolates confirmed as ST398 by multiloc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05559-8
更新日期:2017-07-14 00:00:00
abstract::The aim is to design a patient-specific instrument (PSI) for multilevel cervical pedicle screw placement from C2 to C7, as well as verifying reliability and reproducibility. Computed tomography (CT) scans were obtained from 7 cadaveric cervical spines. Using Mimics software, semiautomatic segmentation was performed fo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51936-w
更新日期:2019-10-30 00:00:00
abstract::Blueberry juice is a healthy and nutritious food that has become increasingly popular worldwide. However, little is known about the microbial groups of this juice that can cause its spoilage. This study aimed to identify the main spoilage microorganisms in blueberry juice and explore whether a microchip pulsed electri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26513-2
更新日期:2018-05-25 00:00:00
abstract::The recent breakthrough in the discovery of Weyl fermions in monopnictide semimetals provides opportunities to explore the exotic properties of relativistic fermions in condensed matter. The chiral anomaly-induced negative magnetoresistance and π Berry phase are two fundamental transport properties associated with the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18674
更新日期:2016-01-04 00:00:00
abstract::A new simple method is proposed to extract the ambipolar diffusion length for two-dimensional (2D) electronic transport in thin film structures using a scanning photoluminescence microscopy (SPLM) setup. No spatially-resolved photoluminescence detection methods are required. By measuring the excitation-position-depend...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62093-w
更新日期:2020-03-23 00:00:00