Abstract:
:The application of electron microscopy to hydrated biological samples has been limited by high-vacuum operating conditions. Traditional methods utilize harsh and laborious sample dehydration procedures, often leading to structural artefacts and creating difficulties for correlating results with high-resolution fluorescence microscopy. Here, we utilize graphene, a single-atom-thick carbon meshwork, as the thinnest possible impermeable and conductive membrane to protect animal cells from vacuum, thus enabling high-resolution electron microscopy of wet and untreated whole cells with exceptional ease. Our approach further allows for facile correlative super-resolution and electron microscopy of wet cells directly on the culturing substrate. In particular, individual cytoskeletal actin filaments are resolved in hydrated samples through electron microscopy and well correlated with super-resolution results.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Wojcik M,Hauser M,Li W,Moon S,Xu Kdoi
10.1038/ncomms8384subject
Has Abstractpub_date
2015-06-11 00:00:00pages
7384issn
2041-1723pii
ncomms8384journal_volume
6pub_type
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