Proximity-dependent initiation of hybridization chain reaction.

Abstract:

:Sensitive detection of protein interactions and post-translational modifications of native proteins is a challenge for research and diagnostic purposes. A method for this, which could be used in point-of-care devices and high-throughput screening, should be reliable, cost effective and robust. To achieve this, here we design a method (proxHCR) that combines the need for proximal binding with hybridization chain reaction (HCR) for signal amplification. When two oligonucleotide hairpins conjugated to antibodies bind in close proximity, they can be activated to reveal an initiator sequence. This starts a chain reaction of hybridization events between a pair of fluorophore-labelled oligonucleotide hairpins, generating a fluorescent product. In conclusion, we show the applicability of the proxHCR method for the detection of protein interactions and posttranslational modifications in microscopy and flow cytometry. As no enzymes are needed, proxHCR may be an inexpensive and robust alternative to proximity ligation assays.

journal_name

Nat Commun

journal_title

Nature communications

authors

Koos B,Cane G,Grannas K,Löf L,Arngården L,Heldin J,Clausson CM,Klaesson A,Hirvonen MK,de Oliveira FM,Talibov VO,Pham NT,Auer M,Danielson UH,Haybaeck J,Kamali-Moghaddam M,Söderberg O

doi

10.1038/ncomms8294

subject

Has Abstract

pub_date

2015-06-12 00:00:00

pages

7294

issn

2041-1723

pii

ncomms8294

journal_volume

6

pub_type

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