Abstract：
:3-Bromopyruvate (3BP) is an energy-depleting drug that inhibits Hexokinase II activity by alkylation during glycolysis, thereby suppressing the production of ATP and inducing cell death. As such, 3BP can potentially serve as an anti-tumorigenic agent. Our previous research showed that 3BP can induce apoptosis via AKT /protein Kinase B signaling in breast cancer cells. Here we found that 3BP can also induce colon cancer cell death by necroptosis and apoptosis at the same time and concentration in the SW480 and HT29 cell lines; in the latter, autophagy was also found to be a mechanism of cell death. In HT29 cells, combined treatment with 3BP and the autophagy inhibitor 3-methyladenine (3-MA) exacerbated cell death, while viability in 3BP-treated cells was enhanced by concomitant treatment with the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp fluoromethylketone (z-VAD-fmk) and the necroptosis inhibitor necrostatin (Nec)-1. Moreover, 3BP inhibited tumor growth in a SW480 xenograft mouse model. These results indicate that 3BP can suppress tumor growth and induce cell death by multiple mechanisms at the same time and concentration in different types of colon cancer cell by depleting cellular energy stores.
journal_name
J Bioenerg Biomembrjournal_title
Journal of bioenergetics and biomembranesauthors
Sun Y,Liu Z,Zou X,Lan Y,Sun X,Wang X,Zhao S,Jiang C,Liu Hdoi
10.1007/s10863-015-9612-1subject
Has Abstractpub_date
2015-08-01 00:00:00pages
319-29issue
4eissn
0145-479Xissn
1573-6881journal_volume
47pub_type
杂志文章abstract：:The mitochondrial UPR (UPRmt) is rapidly gaining attention. While most studies on the UPRmt have focused on its role in aging, emerging studies suggest an important role of the UPRmt in cancer. Further, several of the players of the UPRmt in mammalian cells have well reported roles in the maintenance of the organelle....
journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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doi：10.1007/BF00744683
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00743069
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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doi：10.1007/BF00762524
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journal_title：Journal of bioenergetics and biomembranes
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doi：
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journal_title：Journal of bioenergetics and biomembranes
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doi：10.1007/BF00751049
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journal_title：Journal of bioenergetics and biomembranes
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doi：10.1023/a:1023742002493
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00748766
更新日期：1980-12-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
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更新日期：1994-02-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00785997
更新日期：1991-10-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-012-9433-4
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
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journal_title：Journal of bioenergetics and biomembranes
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更新日期：2002-12-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-013-9504-1
更新日期：2013-06-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-014-9572-x
更新日期：2014-12-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
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更新日期：2012-04-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00763097
更新日期：1994-06-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00743203
更新日期：1981-12-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1005608823087
更新日期：2000-10-01 00:00:00
abstract：:First, halorhodopsin is capable of pumping protons after illumination with green and blue light in the same direction as chloride. Second, mutated bacteriorhodopsin where the proton acceptor Asp85 and the proton donor Asp96 are replaced by Asn showed proton pump activity after illumination with blue light in the same ...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00762676
更新日期：1992-04-01 00:00:00
