Abstract:
:The Drosophila melanogaster sex-peptide (melSP) is a seminal fluid component that induces postmating responses (PMR) of females via the sex-peptide receptor (SPR) . Although SP orthologs are found in many Drosophila species, their functions remain poorly characterized. It is unknown whether SP functions are conserved across species or rather specific to each species. Here, we developed a GFP-tagged melSP (G-SP) and used it to visualize cross-species binding activity to the female reproductive system of various species. First we demonstrated that ectopically expressed G-SP induced PMR in D. melanogaster females and bound to the female reproductive system, most notably to the common oviduct. No binding occurred in the females lacking SPR, indicating that G-SP binding was dependent on SPR. Next we tested whether G-SP binds to the common oviducts from 11 Drosophila species using dissected reproductive tracts. The binding was observed in six species belonging to the D. melanogaster species group, but not to those outside the group. Injection of melSP reduced the receptivity of females belonging to the D. melanogaster species group, but not of those outside the group, being consistent with the ability to bind G-SP. Thus the SP-mediated PMR appears to be limited to this species group. SPR was expressed in the oviducts at high levels in this group; therefore, we speculate that an enhanced expression of SPR in the oviduct was critical to establish the SP-mediated PMR during evolution.
journal_name
Geneticsjournal_title
Geneticsauthors
Tsuda M,Peyre JB,Asano T,Aigaki Tdoi
10.1534/genetics.115.177550subject
Has Abstractpub_date
2015-08-01 00:00:00pages
1161-9issue
4eissn
0016-6731issn
1943-2631pii
genetics.115.177550journal_volume
200pub_type
杂志文章相关文献
GENETICS文献大全abstract::We have studied how a set of male-specific sensory neurons in Caenorhabditis elegans establish axonal connections during postembryonic development. In the adult male, 9 bilateral pairs of ray sensory neurons innervate an acellular fan that serves as a presumptive tactile and olfactory organ during copulation. We visua...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.057000
更新日期:2006-07-01 00:00:00
abstract::CarD is the only reported prokaryotic protein showing structural and functional features typical of eukaryotic high-mobility group A transcription factors. In prokaryotes, proteins similar to CarD appear to be confined primarily to myxobacteria. In Myxococcus xanthus, CarD has been previously shown to act as a positiv...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.029207
更新日期:2004-08-01 00:00:00
abstract::During meiotic prophase, assembly of the synaptonemal complex (SC) brings homologous chromosomes into close apposition along their lengths. The Zip1 protein is a major building block of the SC in Saccharomyces cerevisiae. In the absence of Zip1, SC fails to form, cells arrest or delay in meiotic prophase (depending on...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.071100
更新日期:2007-06-01 00:00:00
abstract::A method for estimating the average level of gene flow among populations is introduced. The method provides an estimate of Nm, where N is the size of each local population in an island model and m is the migration rate. This method depends on knowing the phylogeny of the nonrecombining segments of DNA that are sampled...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::Sequences of Bronze2 (Bz2), a maize gene which is required for the synthesis of the purple pigment anthocyanin, have been cloned by combining the techniques of transposon tagging and differential hybridization. First, a mutable bz2 allele (bz2-mu1) was recovered from a Mutator line. The mutation was assumed to result ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract::Genome-wide association studies (GWAS) have been widely used for identifying common variants associated with complex diseases. Despite remarkable success in uncovering many risk variants and providing novel insights into disease biology, genetic variants identified to date fail to explain the vast majority of the heri...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.185009
更新日期:2016-03-01 00:00:00
abstract::Two strains of Drosophila melanogaster derived from different geographical localities were subjected to selection for fast and slow developmental rate. Four enzyme polymorphisms were monitored for their response to such selection. Significant changes in allele frequencies were effected for the alphaGpdh and 6Pgd polym...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
abstract::The mutation process at microsatellite loci typically occurs at high rates and with stepwise changes in allele sizes, features that may introduce bias when using classical measures of population differentiation based on allele identity (e.g., F(ST), Nei's Ds genetic distance). Allele size-based measures of differentia...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-04-01 00:00:00
abstract::At least six nuclear loci are responsible for the genetic control of malate dehydrogenase (L-malate: NAD oxidoreductase; EC 1.1.1.37; MDH) in coleoptiles of maize. Three independently segregating loci (Mdh1, Mdh2, Mdh3) govern the production of MDH isozymes resistant to inactivation by ascorbic acid and found largely ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
abstract::In Saccharomyces cerevisiae, genes located at the telomeres and the HM loci are subject to transcriptional silencing. Here, we report results of screening a Gal4 DNA-binding domain hybrid library for proteins that cause silencing when targeted to a silencer-defective HMR locus. ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.166.1.631
更新日期:2004-01-01 00:00:00
abstract::Escherichia coli K12 does not metabolize beta-glucosides such as arbutin and salicin because of lack of expression of the bglBSRC operon, which contains structural genes for transport (bglC) and hydrolysis (bglB) of phospho-beta-glucosides. Mutants carrying lesions in the cis-acting regulatory site bglR metabolize bet...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract::Sex-specific differences in dispersal, survival, reproductive success, and natural selection differentially affect the effective population size (N(e)) of genomic regions with different modes of inheritance such as sex chromosomes and mitochondrial DNA. In papionin monkeys (macaques, baboons, geladas, mandrills, drill...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.116228
更新日期:2010-07-01 00:00:00
abstract::This paper describes a perturbation experiment on the frequency of the F and S Alcohol dehydrogenase (Adh) alleles of D. melanogaster. Fifty-four iso-female lines set up from three wild populations and with initial F frequencies of either 0.25, 0.50 or 0.75 were maintained on standard laboratory food medium at 22 degr...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::LARK is an essential Drosophila RNA-binding protein of the RNA recognition motif (RRM) class that functions during embryonic development and for the circadian regulation of adult eclosion. LARK protein contains three consensus RNA-binding domains: two RRM domains and a retroviral-type zinc finger (RTZF). To show that ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::In the Brassicaceae, self-vs. nonself-recognition in self-incompatibility is controlled by sporophytic S-alleles. Haplotypes specifying both SRK (S-receptor kinase) and SLG (S-locus glycoprotein) are considered to play an important role in the recognition reactions. We compared the nucleotide sequences of SRK9(Bc) and...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
abstract::Transcriptional silencing involves the formation of specialized repressive chromatin structures. Previous studies have shown that the histone H3-H4 chaperone known as chromatin assembly factor 1 (CAF-1) contributes to transcriptional silencing in yeast, although the molecular basis for this was unknown. In this work w...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.054783
更新日期:2006-06-01 00:00:00
abstract::N6-methyladenosine (m6A), catalyzed by Mettl3 methyltransferase, is a highly conserved epigenetic modification in eukaryotic messenger RNA (mRNA). Previous studies have implicated m6A modification in multiple biological processes, but the in vivo function of m6A has been difficult to study, because mettl3 mutants are ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.117.300574
更新日期:2018-02-01 00:00:00
abstract::Allozyme and inversion data from natural populations of Drosophila melanogaster from the eastern United States were analyzed to determine whether the clines at allozyme loci are due to nonrandom associations with common cosmopolitan inversions. All inversions show strong clines. Clines were large and significant for h...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1978-03-01 00:00:00
abstract::Immunosenescence, the age-related decline in immune system function, is a general hallmark of aging. While much is known about the cellular and physiological changes that accompany immunosenescence, we know little about the genetic influences on this phenomenon. In this study we combined age-specific measurements of b...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.112.140640
更新日期:2012-07-01 00:00:00
abstract::The centromere is essential for proper segregation and inheritance of genetic information. Centromeres are generally regulated to occur exactly once per chromosome; failure to do so leads to chromosome loss or damage and loss of linked genetic material. The mechanism for faithful regulation of centromere activity and ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::Mutation frequency and specificity were determined as a function of age in nuclear DNA from liver, bladder, and brain of Big Blue lacI transgenic mice aged 1.5-25 months. Mutations accumulated with age in liver and accumulated more rapidly in bladder. In the brain a small initial increase in mutation frequency was obs...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
abstract::Using an insertional mutagenesis approach, a series of Neurospora crassa mutants affected in the ability to control entry into the conidiation developmental program were isolated. One such mutant, GTH16-T4, was found to lack normal vegetative hyphae and to undergo constitutive conidiation. The affected gene has been n...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract::Dauer formation in Caenorhabditis elegans is induced by chemosensation of high levels of a constitutively secreted pheromone. Seven genes defined by mutations that confer a dauer-formation constitutive phenotype (Daf-c) can be congruently divided into two groups by any of three criteria. Group 1 genes (daf-11 and daf-...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
abstract::Resolving the mechanistic and genetic bases of reproductive barriers between species is essential to understanding the evolutionary forces that shape speciation. Intrinsic hybrid incompatibilities are often treated as fixed between species, yet there can be considerable variation in the strength of reproductive isolat...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.300840
更新日期:2018-07-01 00:00:00
abstract::Regulation of transcription can be a complex process in which many cis- and trans-interactions determine the final pattern of expression. Among these interactions are trans-interactions mediated by the pairing of homologous chromosomes. These trans-effects are wide ranging, affecting gene regulation in many species an...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.133231
更新日期:2011-11-01 00:00:00
abstract::The satellite DNA's from the embryos of five species of Drosophila (D. melanogaster, D. simulans, D. nasuta, D. virilis and D. hydei) have been analyzed for the presence of closed circular duplex DNA molecules, as determined by CsCl-EBr gradients. Circular DNA molecules were found in every species but D. melanogaster....
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1972-11-01 00:00:00
abstract::The initiation of growth cessation and dormancy represents a critical ecological and evolutionary trade-off between survival and growth in most forest trees. The most important environmental cue regulating the initiation of dormancy is a shortening of the photoperiod and phytochrome genes have been implicated in short...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.047522
更新日期:2006-03-01 00:00:00
abstract::Triose phosphate isomerase (TPI) deficiency glycolytic enzymopathy is a progressive neurodegenerative condition that remains poorly understood. The disease is caused exclusively by specific missense mutations affecting the TPI protein and clinically features hemolytic anemia, adult-onset neurological impairment, degen...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.087551
更新日期:2008-06-01 00:00:00
abstract::The DNA-binding protein encoded by the zeste gene of Drosophila activates transcription and mediates interchromosomal interactions such as transvection. The mutant protein encoded by the zeste1 (z1) allele retains the ability to support transvection, but represses white. Similar to transvection, repression requires Ze...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::The construction in bacteriophage lambda of a set of long DNA palindromes with paired changes in the central sequence is described. Identical palindrome centers were previously used by others to test the S-type model for cruciform extrusion in vitro. Long DNA palindromes prevent the propagation of carrier phage lambda...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00