Abstract:
OBJECTIVE:To explore the role of miR-214 in the progression of hepatocellular carcinoma (HCC) and its inhibitory mechanisms in depressing the signaling pathway of β-catenin, this study was conducted. METHODS:We ectopically expressed miR-214 in HepG2 cells to obtain cell lines Lv-miR-214-HepG2 and their control Lv-control-HepG2. Differences between the two cell lines were compared in cell growth, proliferation, colony forming ability and cell cycles. RT-PCR method was applied for the quantification of β-catenin mRNA expression. Western-blot method was applied for the determination of the protein level of β-catenin and their downstream targets (ie. Cyclin D1, c-Myc and TCF-1). The effect of miR-214 on cells was further explored through RNA interference and restoring miR-214 expression. RESULTS:In comparison with negative (Lv-control-HepG2) and blank (HepG2) control, a significant inhibition of cell growth and proliferation caused by miR-214 was observed after 48∼72h of cell culture experiments (P<0.05). The miR-214 treatment resulted in a colony forming efficiency of (23.28±3.26)%, which was significantly lower than that of negative control [(51.31±3.97)%] (P<0.05). According to FCM results, the experimental group, compared with control, showed a higher proportion of cells in G0/G1 phase [(70.32±3.12)%] but a lower proportion in S phase [(18.42±2.90)%] (P<0.05). The MTT assay demonstrated a significant inhibition of the proliferation and β-catenin expression of HCC cells compared with control (P<0.05), while no significant difference was observed after HCC cells being transfected with β-catenin overexpression plasmid (P>0.05). By comparing to the RT-PCR and Western-blot results of control, the miR-214 treatment led to a slightly decrease in the β-catenin mRNA expression (P>0.05), but an extremely inhibition in the protein level of β-catenin and its downstream targets Cyclin D1, c-Myc, and TCF-1 (P<0.05). CONCLUSIONS:miR-214 functions as a suppressor during the progression of HCC, and its inhibitory role was achieved by down-regulating β-catenin signaling pathway.
journal_name
Asian Pac J Trop Medjournal_title
Asian Pacific journal of tropical medicineauthors
Zhang LL,Guo YJ,Zhao CN,Gao JYdoi
10.1016/S1995-7645(14)60350-3subject
Has Abstractpub_date
2015-05-01 00:00:00pages
392-8issue
5eissn
1995-7645issn
2352-4146pii
S1995-7645(14)60350-3journal_volume
8pub_type
杂志文章abstract:OBJECTIVE:To determine the content of benzyl glucosinolate (BG) in the pulp and the seed and investigate the anti-cancer activity of its hydrolysis product in Carica papaya L. METHODS:Determination of BG was performed on an Hypersil BDS C(18) column at the wavelength of 214 nm with 0.1% trifluoroacetic acid (TFA) aque...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(12)60030-3
更新日期:2012-03-01 00:00:00
abstract:OBJECTIVE:To compare the anti-atherosclerotic effects of two different extracts from the leaves of Mallotus furetianus by using rat model of atherosclerosis. METHODS:The air-dried powdered Mallotus furetianus leaves were extracted with ethanol and then evaporated. The ethanol extract was experienced Diaion HP-20 CC wi...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60212-5
更新日期:2011-11-01 00:00:00
abstract:OBJECTIVE:To investigate ultrastructural changes in pulmonary tissue of a rat model of pulmonary fibrosis following treatment with compound Carapax trionycis (C. trionycis; Biejia in Chinese) formula. METHODS:Sixty male Sprague-Dawley rats were randomly divided into four groups (n=15): compound C. trionycis formula hi...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(13)60013-9
更新日期:2013-02-01 00:00:00
abstract:OBJECTIVE:To study the involvement of variations in 4 genes associated with susceptibility and/or protection against HIV-1 in serodiscordant couples in Burkina Faso, namely, genes encoding HLA-B57, interferon regulatory factor 1 (IRF1), dendritic cell-specific ICAM3-grabbing nonintegrin (DC-SIGN) and CCR5 delta 32 (CCR...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(14)60211-X
更新日期:2014-09-01 00:00:00
abstract:OBJECTIVE:To evaluate the antihyperglycemic activity of leaves of Hyptis suaveolens using streptozotocin model. METHODS:Hyptis suaveolens extract (HSE) 250 and 500 mg/kg body weight was administered orally to streptozotocin induced diabetes, once daily for 21 days. RESULTS:A significant reduction in blood glucose was...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60175-2
更新日期:2011-09-01 00:00:00
abstract:OBJECTIVE:To invest the differences among mesenchymal stem cells (MSCs) derived from different tissues and their impacts on clinical applications. METHODS:In this study, MSCs were isolated from adipose tissue (AD), umbilical cord tissue (UC), and menstrual blood (Men) and compared their biological characteristics in t...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2015.07.022
更新日期:2015-09-01 00:00:00
abstract:OBJECTIVE:To investigate the diuretic, hypotensive and renal effect of Opuntia ficus-indica in two different species in oral and intravenous administration. METHODS:Diuretic activity was evaluated in rats with the plant cladode gel and aqueous extract administrated orally, and was evaluated in rabbits with plant extra...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2017.08.016
更新日期:2017-09-01 00:00:00
abstract::The 2014 Ebola outbreak in West Africa, primarily affecting Guinea, Sierra Leone, and Liberia, has exceeded all previous Ebola outbreaks in the number of cases and in international response. Although infections only occur frequently in Western Africa, the virus has the potential to spread globally and is classified as...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章,评审
doi:10.1016/j.apjtm.2015.09.001
更新日期:2015-10-01 00:00:00
abstract:OBJECTIVES:To do mapping and modeling of conformational B cell epitope regions of highly conserved and protective regions of three merozoitecandidate vaccine proteins of Plasmodium vivax (P. vivax), ie. merozoite purface protein-1 (PvMSP-1), apical membrane antigen -1 domain ∏ (PvAMA1-D∏) and region ∏ of the Duffy bind...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(14)60146-2
更新日期:2014-10-01 00:00:00
abstract:OBJECTIVE:To explore the effect and mechanism of angiotensin II receptor blockers - Irbesartan on occurrence of ventricular arrhythmias in rats with myocardial ischemia. METHODS:Rats with embryonic cardiomyocytes-H9c2 were randomly divided into control group, ischemia group, Irbesartan group and Irbesartan + ischemia ...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2016.07.022
更新日期:2016-10-01 00:00:00
abstract:OBJECTIVE:To build the rat model of gastric precancerous lesions and discuss the effect of transplantation of mesenchymal stem cells (BMMSCs) on the pathological change. METHODS:The rat model of gastric precancerous lesions was built using N-methyl-N'-nitro-N'-nitrosoguanidine. After the intravenous transplantation of...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章,撤回出版物
doi:10.1016/j.apjtm.2015.11.006
更新日期:2015-12-01 00:00:00
abstract:OBJECTIVE:To assess the prevalence of malaria helminth co-infections and their contribution for aneamia in febrile patients attending Azzezo health center, Gondar, Northwest Ethiopia. METHODS:A cross section study was conducted among febrile patients attending Azezo health center from February-March 30, 2011. Convenie...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(12)60147-3
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVE:To determine the range and incidence of complications associated with Plasmodium vivax (P. vivax) malaria. METHODS:A retrospective analysis was performed of all patients of P. vivax malaria admitted in Kasturba Medical College, Manipal between January and December, 2010. Patients with mixed malarial infectio...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60251-4
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:To analyze the protective efficacy of recombinant 78 kDa antigen of Leishmania donovani in combination with two adjuvants, that is, cationic liposomes or MPL-A against visceral leishmaniasis in BALB/c mice. METHODS:The genomic DNA of promastigotes was isolated and 583 bp of T cell epitopes of gene encoding 7...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2015.06.008
更新日期:2015-07-01 00:00:00
abstract::Clostridium difficile is a Gram-positive, strictly anaerobic, spore-forming bacterium. It is the most common cause of antibiotic-associated diarrhea in hospitals and other healthcare facilities and is of significant concern because of the increasing morbidity and mortality rates as well as increased health care costs....
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(14)60197-8
更新日期:2014-09-01 00:00:00
abstract:OBJECTIVE:To validate traditional claims of usefulness of the Indian plants in management of poisonous snakebite and evaluate the antivenom properties displayed by the alcoholic extracts of Andrographis paniculata (A. paniculata), Crateva magna (C. magna), Gloriosa superba (G. superba) and Hydrocotyle javanica (H. java...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60185-5
更新日期:2011-09-01 00:00:00
abstract:OBJECTIVE:To explore the regulation effect of Rheum palmatum (R. palmatum) L. on NF-κB signaling pathway of ALF mice. METHODS:The intraperitoneal injection of d-GalN/LPS was employed for the model building. Mice in the treatment group and positive control group were given the R. palmatum L. and bifendate before the mo...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2015.09.011
更新日期:2015-10-01 00:00:00
abstract::Captive reptiles, always more often present in domestic environment as pets, may harbor and excrete a large variety of zoonotic pathogens. Among them, Salmonella is the most well-known agent, whereas there are very scant data about infections by mycobacteria, chlamydiae and leptospirae in cold-blooded animals. However...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章,评审
doi:10.1016/j.apjtm.2017.07.020
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVE:To delineate the frequency of occurrence of bla(CTX-M), bla(TEM), and bla(SHV) in Enterobacteriaceae from North-Indian tertiary hospital. METHODS:A random collection of a subset of 45 Escherichia coli (E. coli) and 28 Klebsiella pneumoniae (K. pneumoniae) that was resistant to a third generation cephalospori...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60046-1
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVE:To study the effects of aqueous extract of Gmelina arborea bark on normoglycemic levels and streptozotocin (STZ) induced diabetes in rats. METHODS:After single administration of the aqueous extract, plasma glucose level was determined up to 6 h. In subacute study, the aqueous extract was administered for 28 ...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(13)60104-2
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVE:To investigation the chemopreventive potential of Fumaria indica (F. indica) extract (FIE) on N-nitrosodiethylamine and CCl(4)-induced hepatocarcinogenesis in Wistar rats. METHODS:The experimental animals were divided into six groups (n=6). Hepatocellular carcinoma was induced by single intraperitoneal injec...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(12)60128-X
更新日期:2012-08-01 00:00:00
abstract::A natural prodrug is a chemical compound or substance obtained from plants, microorganism, animal and marine sources. Natural products are small molecule source for Food and Drug Administration approved drugs and major sources for drug discovery. Most of the drugs for different ailment diseases undergo first pass meta...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(14)60203-0
更新日期:2014-09-01 00:00:00
abstract:OBJECTIVE:To investigate the levels of serum cortisol in patients with uncomplicated Plasmodium falciparum (P. falciparum) malaria in an area of unstable malaria transmission in eastern Sudan. METHODS:The concentrations of cortisol were measured in sera of 25 patients with uncomplicated P. falciparum malaria (at prese...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60056-4
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVE:To investigate the efficiency of β-galactosidase gene transfer into rat kidney with ultrasound-mediated microbubble destruction via different injection routes. METHODS:A total of 25 Wistar rats were randomly divided into 5 groups. Four groups received a mixture of optison microbubbles (0.2 mL) and lacz plasm...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(12)60099-6
更新日期:2012-07-01 00:00:00
abstract:OBJECTIVES:To test the hypothesis that concentration of amniotic fluid alpha-fetal protein (AFAFP) is increased in thalassemia fetus. METHODS:A total of 135 cases of amniocentesis admitted from July 2013 to December 2014 were included in this study. Among them 98 cases of normal fetuses were assigned into control grou...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2017.01.001
更新日期:2017-02-01 00:00:00
abstract:OBJECTIVE:To investigate the antibacterial and antioxidant activities of hydroalcoholic extract of Schotia latifolia (S. latifolia) bark commonly used in South Africa traditional medicine for the treatment of various ailments. METHODS:The antibacterial test and MIC was determined by using agar well diffusion and dilut...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60225-3
更新日期:2011-12-01 00:00:00
abstract::Salmonella are most commonly associated with gastroenteritis and enteric fever in humans. Occasionally, dissemination of bacilli throughout the body results in establishment of localized foci of persistent infection especially in patients with debilitating diseases and immunosuppressive states. Infection at various ab...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60117-X
更新日期:2011-05-01 00:00:00
abstract::We present an atypical case of disseminated cutaneous leishmaniasis in the Sihuas district, located in the Andean valleys of Ancash-Peru. A 62-year-old man with no particular medical history presented multiple lesions located on the inferior abdomen, lumbar region and the right anterior thigh. Histological analysis fo...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2017.10.001
更新日期:2017-11-01 00:00:00
abstract:OBJECTIVE:To evaluate the pharmacognostic characters of Limonia acidissima L. (L. acidissima) leaf and stem, an important traditional medicinal plant. METHODS:The present study provides pharmacognostic, physicochemical and phytochemical details of leaf and stem of L. acidissima. Micro and macroscopic characters were a...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(14)60240-6
更新日期:2014-09-01 00:00:00
abstract:OBJECTIVE:To evaluate a new pharmacological activity/effect of linolenic acid (α- and γ-form) and conjugated-linoleic acid (CLA) causing antibacterial activity against Mycobacterium tuberculosis (Mtb). METHODS:The anti-Mtb activity/effect of linolenic acid and CLA were determined using different anti-Mtb indicator met...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2016.01.021
更新日期:2016-02-01 00:00:00