Abstract:
:Cytotoxic T lymphocytes (CTLs) use polarized secretion to rapidly destroy virally infected and tumor cells. To understand the temporal relationships between key events leading to secretion, we used high-resolution 4D imaging. CTLs approached targets with actin-rich projections at the leading edge, creating an initially actin-enriched contact with rearward-flowing actin. Within 1 min, cortical actin reduced across the synapse, T cell receptors (TCRs) clustered centrally to form the central supramolecular activation cluster (cSMAC), and centrosome polarization began. Granules clustered around the moving centrosome within 2.5 min and reached the synapse after 6 min. TCR-bearing intracellular vesicles were delivered to the cSMAC as the centrosome docked. We found that the centrosome and granules were delivered to an area of membrane with reduced cortical actin density and phospholipid PIP2. These data resolve the temporal order of events during synapse maturation in 4D and reveal a critical role for actin depletion in regulating secretion.
journal_name
Immunityjournal_title
Immunityauthors
Ritter AT,Asano Y,Stinchcombe JC,Dieckmann NM,Chen BC,Gawden-Bone C,van Engelenburg S,Legant W,Gao L,Davidson MW,Betzig E,Lippincott-Schwartz J,Griffiths GMdoi
10.1016/j.immuni.2015.04.013subject
Has Abstractpub_date
2015-05-19 00:00:00pages
864-76issue
5eissn
1074-7613issn
1097-4180pii
S1074-7613(15)00173-9journal_volume
42pub_type
杂志文章相关文献
IMMUNITY文献大全abstract::Recent experiments have suggested that tumor necrosis factor alpha (TNFalpha) can down-regulate islet-specific T cells and prevent the development of autoimmune diabetes. Here we demonstrate that transgenic mice expressing both TNFalpha and the Leishmania major LACK antigen in the pancreas (RIP-TNFalpha/RIP-LACK) exhi...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/s1074-7613(00)80361-1
更新日期:1997-09-01 00:00:00
abstract::The stromal scaffold of the lymph node (LN) paracortex is built by fibroblastic reticular cells (FRCs). Conditional ablation of lymphotoxin-β receptor (LTβR) expression in LN FRCs and their mesenchymal progenitors in developing LNs revealed that LTβR-signaling in these cells was not essential for the formation of LNs....
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2013.03.012
更新日期:2013-05-23 00:00:00
abstract::In a recent issue of Nature, Borges da Silva et al. (2018) reveal that P2RX7, a receptor for extracellular ATP, promotes CD8 T cell memory by enhancing metabolic fitness. This work links an ancient "danger" signal with long-term immunity. ...
journal_title:Immunity
pub_type: 评论,杂志文章
doi:10.1016/j.immuni.2018.08.009
更新日期:2018-08-21 00:00:00
abstract::Differentiation of naive T cells into mature Th2 cells is associated with the appearance of a complex pattern of DNase I hypersensitive (DH) sites within the IL-4/IL-13 cytokine gene cluster. We show here that targeted deletion of an inducible DH site, V(A), and the adjacent conserved DH site V (CNS-2) selectively com...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/s1074-7613(02)00334-5
更新日期:2002-07-01 00:00:00
abstract::Diet-induced obesity can induce low-level inflammation and insulin resistance. Interleukin-1β (IL-1β) is one of the key proinflammatory cytokines that contributes to the generation of insulin resistance and diabetes, but the mechanisms that regulate obesity-driven inflammation are ill defined. Here we found reduced ex...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2014.11.013
更新日期:2014-12-18 00:00:00
abstract::For a brief period during fetal lymph node organogenesis in mice, lymph node postcapillary high endothelial venules surprisingly express the Peyer's patch addressin MAdCAM-1. This expression allows initial seeding of this incipient structure by two unusual lymphocyte populations selectively expressing the Peyer's patc...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/s1074-7613(00)80371-4
更新日期:1997-10-01 00:00:00
abstract::De Libero et al. (2005) demonstrate in this issue of Immunity that bacterial infection leads to increased synthesis of autologous glycolipids that are recognized by CD1-restricted human T cells, indicating that recognition of inducible self-glycolipids could be a mechanism for microbial detection. This mechanism also ...
journal_title:Immunity
pub_type: 评论,杂志文章
doi:10.1016/j.immuni.2005.06.001
更新日期:2005-06-01 00:00:00
abstract::Inflammation mediated by antibody-antigen complexes contributes to autoimmune diseases. Mice deficient in the common Fcgamma-chain are protected from IgG-mediated glomerulonephritis and the reverse passive Arthus (RPA) reaction and FcR-bearing macrophages, and mast cells have been assigned primary roles in these proce...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2008.04.013
更新日期:2008-06-01 00:00:00
abstract::Some endocrine organs are frequent targets of autoimmune attack. Here, we addressed the origin of autoimmune disease from the viewpoint of feedback control. Endocrine tissues maintain mass through feedback loops that balance cell proliferation and removal according to hormone-driven regulatory signals. We hypothesized...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2020.04.022
更新日期:2020-05-19 00:00:00
abstract::Lineage commitment during myelopoiesis has been linked to late-stage progenitors with multi-lineage potential. In a recent issue of Cell, Paul et al. (2015) now suggest a much earlier commitment toward distinct lineages even prior to the common myeloid progenitor state. ...
journal_title:Immunity
pub_type: 评论,杂志文章
doi:10.1016/j.immuni.2015.12.019
更新日期:2016-01-19 00:00:00
abstract::Conserved molecular patterns derived from pathogenic microorganisms prime antigen-presenting dendritic cells (DC) to induce adaptive T cell responses. In contrast, virus-infected or tumor cells that express low levels of major histocompatibility complex (MHC) class I activate natural killer (NK) cells for direct killi...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/s1074-7613(03)00264-4
更新日期:2003-10-01 00:00:00
abstract::Lipopolysaccharide binding protein (LBP) has a well-established role in LPS-induced immune responses. Here, we report that LBP also plays an essential role in the innate immune response to Gram-positive pneumococci, specifically to their major inflammatory component, pneumococcal cell wall (PCW). LBP was present in th...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/s1074-7613(03)00205-x
更新日期:2003-08-01 00:00:00
abstract::Although cellular receptors for immunoglobulins were first identified nearly 40 years ago, their central role in the immune response was discovered only in the last decade. They are key players in both the afferent and efferent phase of an immune response, setting thresholds for B cell activation, regulating the matur...
journal_title:Immunity
pub_type: 杂志文章,评审
doi:10.1016/j.immuni.2005.11.010
更新日期:2006-01-01 00:00:00
abstract::The transcriptional repressor Blimp-1 is considered the master regulator of plasma-cell differentiation. In this issue of Immunity, Kallies et al. (2007) identify a Blimp-1-independent stage of differentiation, followed by Blimp-1-dependent terminal differentiation. ...
journal_title:Immunity
pub_type: 评论,杂志文章
doi:10.1016/j.immuni.2007.05.005
更新日期:2007-05-01 00:00:00
abstract::The Notch2 ligand DL1 is required for marginal zone B cell development. In this issue of Immunity, Tan et al. (2009) show that modification of Notch2 by Fringe enzymes is critical to maturing B cells for accessing DL1 on vascular endothelial cells. ...
journal_title:Immunity
pub_type: 评论,杂志文章
doi:10.1016/j.immuni.2009.01.003
更新日期:2009-02-20 00:00:00
abstract::Crosspresentation of exogenous antigens (Ags) to CD8(+) T cells by dendritic cells generally requires their entry into the cytosol. Here we show that both soluble and phagocytosed extracellular Ags accessed the cytosol via molecular components required for endoplasmic reticulum (ER)-associated degradation (ERAD). Exog...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2006.08.017
更新日期:2006-10-01 00:00:00
abstract::The presence of CD4+ T cells in the healthy brain parenchyma remains controversial due to the barrier function of the glia limitans. Pasciuto, Burton, Roca et al. in Cell describe the dynamic recruitment of CD4+ T cells within the brain parenchyma, their unexpected contribution to microglial maturation, and, ultimatel...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2020.08.011
更新日期:2020-09-15 00:00:00
abstract::Genetic variation influences how the genome is interpreted in individuals and in mouse strains used to model immune responses. We developed approaches to utilize next-generation sequencing datasets to identify sequence variation in genes and enhancer elements in congenic and backcross mouse models. We defined genetic ...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2019.05.006
更新日期:2019-07-16 00:00:00
abstract::Memory T cells provide long-lasting protective immunity, and distinct subpopulations of memory T cells drive chronic inflammatory diseases such as asthma. Asthma is a chronic allergic inflammatory disease with airway remodeling including fibrotic changes. The immunological mechanisms that induce airway fibrotic change...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2018.04.023
更新日期:2018-07-17 00:00:00
abstract::Sterile inflammation can be initiated by innate immune recognition of markers of tissue injury termed damage-associated molecular patterns (DAMPs). DAMP recognition by dendritic cells (DCs) has also been postulated to lead to T cell responses to foreign antigens in tumors or allografts. Many DAMPs represent intracellu...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2012.03.008
更新日期:2012-04-20 00:00:00
abstract::A major mechanism maintaining immune tolerance is the deletion of potentially autoreactive thymocytes by apoptosis during development in the thymus. Previous reports suggest that apoptosis is induced by high avidity signals transduced via the T cell receptor; however, the role of signals transduced by other cell surfa...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/s1074-7613(00)80275-7
更新日期:1996-12-01 00:00:00
abstract::Peptide-specific down-regulation of T cell responses may represent a powerful tool to intervene in autoimmune diseases or graft rejections. It is therefore important to know whether peptide treatment tolerizes both naive and antigen-experienced memory T lymphocytes. Here we show that a major histocompatibility complex...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/s1074-7613(00)80340-4
更新日期:1997-05-01 00:00:00
abstract::In this issue of Immunity, Xu et al. reveal that dermal dendritic cells produce interleukin-31, which acts on neurons to promote wound itch. Their findings link itch associated with deeper wounds-wounds that extend beyond the epithelium-to the cells and cytokines that mediate wound healing. ...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2020.07.016
更新日期:2020-08-18 00:00:00
abstract::Phosphatidylinositol 3' OH kinase (PI3K) signaling and FOXO transcription factors play opposing roles at several B cell developmental stages. We show here abundant nuclear FOXO1 expression in the proliferative compartment of the germinal center (GC), its dark zone (DZ), and PI3K activity, downregulating FOXO1, in the ...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2015.10.021
更新日期:2015-12-15 00:00:00
abstract::Description of macrophage activation is currently contentious and confusing. Like the biblical Tower of Babel, macrophage activation encompasses a panoply of descriptors used in different ways. The lack of consensus on how to define macrophage activation in experiments in vitro and in vivo impedes progress in multiple...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2014.06.008
更新日期:2014-07-17 00:00:00
abstract::Specification of the T helper 17 (Th17) cell lineage requires a well-defined set of transcription factors, but how these integrate with posttranscriptional and epigenetic programs to regulate gene expression is poorly understood. Here we found defective Th17 cell cytokine expression in miR-155-deficient CD4+ T cells i...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2014.03.014
更新日期:2014-06-19 00:00:00
abstract::Why HIV-specific CD8(+) T cells ultimately fail to clear or control HIV infection is not known. We show here that HIV-specific CD8(+) T cells exhibit increased sensitivity to CD95/Fas-induced apoptosis. This apoptosis is 3-fold higher compared to CMV-specific CD8(+) T cells from the same patients. HIV-specific CD8(+) ...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/s1074-7613(01)00246-1
更新日期:2001-12-01 00:00:00
abstract::Select humans and animals control persistent viral infections via adaptive immune responses that include production of neutralizing antibodies. The precise genetic basis for the control remains enigmatic. Here, we report positional cloning of the gene responsible for production of retrovirus-neutralizing antibodies in...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2017.07.013
更新日期:2017-08-15 00:00:00
abstract::Mutations in the NALP3/CIAS1/cryopyrin gene are linked to three autoinflammatory disorders: Muckle-Wells syndrome, familial cold autoinflammatory syndrome, and chronic infantile neurologic cutaneous and articular syndrome. NALP3, with the adaptor molecule ASC, has been proposed to form a caspase-1-activating "inflamma...
journal_title:Immunity
pub_type: 杂志文章
doi:10.1016/j.immuni.2006.02.004
更新日期:2006-03-01 00:00:00
abstract::In this issue of Immunity, Spangler et al. and Mitra et al. demonstrate how structural changes in the IL-2 molecule alter interactions with the IL-2 receptor, leading to differential cellular targeting and biochemical responses and selective immune consequences. ...
journal_title:Immunity
pub_type: 评论,杂志文章
doi:10.1016/j.immuni.2015.05.002
更新日期:2015-05-19 00:00:00