Abstract：
:A new brain-cell line derived from Japanese flounder Paralichthys olivaceus (POBC) was established. POBC was subcultured for 67 passages over the course of 420 days. The cultured cells were primarily epithelioid-like. Chromosome analysis revealed the cell line to possess the normal P. olivaceus diploid karyotype of 2n = 48t (telocentric chromosomes). The cells exhibited the astrocyte marker glial fibrillary acidic protein by immunocytochemistry, and significant fluorescent signals were observed when the cells were transfected with green fluorescent protein reporter plasmid. The established POBC would be ideal material for the study of function of fish ependyma, the central neuroendocrine system and endocrine disruptors in the marine environment.
journal_name
J Fish Bioljournal_title
Journal of fish biologyauthors
Zheng Y,Peng LM,You F,Zou YX,Zhang PJ,Chen SLdoi
10.1111/jfb.12700subject
Has Abstractpub_date
2015-07-01 00:00:00pages
115-22issue
1eissn
0022-1112issn
1095-8649journal_volume
87pub_type
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