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Dacarbazine as a minor groove binder of DNA: Spectroscopic, biophysical and molecular docking studies.

Abstract：

:A detailed investigation on the mode of action and binding mechanism of a potent anticancer drug, 5-(3,3-dimethyl-1-triazeno)-imidazole-4-carboxamide (DCR) with calf thymus DNA (ctDNA) was carried out. UV-vis and fluorescence spectrophotometry suggested the formation of complex between DCR and ctDNA. The binding constant (Kb) determined by ITC was 7.89×10(4) M(-1). Thermodynamic parameters obtained by isothermal calorimetry suggested the spontaneous free energy (ΔG<0) and large favorable enthalpy driven (ΔH<0) reaction, indicating the key role of hydrogen bonding and van der Waals forces in groove binding process. Moreover, DNA-melting and circular dichroism as well as competitive displacement studies with ethidium bromide, Hoechst 33258 and potassium iodide, clearly established the formation of a groove binding system between the DCR and ctDNA. Molecular docking analysis further confirmed DCR to be a minor groove binder involving hydrogen bonding mediated 'A-T'-rich region of 'B-DNA'.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Ahmad I,Ahmad M

doi

10.1016/j.ijbiomac.2015.04.055

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

193-200

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(15)00293-7

journal_volume

79

pub_type

杂志文章

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