MIRA-seq for DNA methylation analysis of CpG islands.

abstract：:Proper immune function is the result of multiple cell commitment and differentiation steps, and adequate control of activation mechanisms. Deregulation of transcriptional programs in immune cells leads to the development of hematological malignancies, autoimmune diseases or immunodeficiencies. In this sense, epigeneti...

journal_title：Epigenomics

authors： Rodriguez-Cortez VC,Hernando H,de la Rica L,Vento R,Ballestar E

更新日期：2011-12-01 00:00:00

abstract：AIM:There is a growing concern about the potential adverse effects of high dose folic acid (FA) supplementation before and during pregnancy. FA metabolism generates S-adenosyl methionine (SAM) which is an important cofactor of epigenetic programming. We sought to assess the impact of a large dose of SAM on early embryo...

journal_title：Epigenomics

authors： Shojaei Saadi HA,Gagné D,Fournier É,Baldoceda Baldeon LM,Sirard MA,Robert C

更新日期：2016-08-01 00:00:00

abstract：AIM:This study aims to couple DNA methylation changes and evolution of retrogenes. MATERIALS & METHODS:A new two-step strategy was developed to screen retrogenes. Further, reduced representation bisulfite sequencing and RNA-seq data of eight tissues were used to analyze retrogenes. RESULTS:A total of 964 retrocopies ...

journal_title：Epigenomics

authors： Fang C,Zou C,Fu Y,Li J,Li Y,Ma Y,Zhao S,Li C

更新日期：2018-02-01 00:00:00

abstract：AIM:To assess the correlation between KDM6B and estrogen receptor β (ERβ) expression in malignant pleural mesothelioma (MPM). MATERIALS & METHODS:We evaluated gene expression by in silico analysis of microarray data, real-time PCR and western blot in MPM tumors and cell lines. RESULTS & CONCLUSION:We report a strong ...

journal_title：Epigenomics

authors： Manente AG,Pinton G,Zonca S,Tavian D,Habib T,Jithesh PV,Fennell D,Nilsson S,Moro L

更新日期：2016-09-01 00:00:00

abstract：:The examination of potential roles of epigenetic alterations in the pathogenesis of psychotic diseases have become an essential alternative in recent years as genetic studies alone are yet to uncover major gene(s) for psychosis. Here, we describe the current state of knowledge from the gene-specific and genome-wide st...

journal_title：Epigenomics

authors： Abdolmaleky HM,Zhou JR,Thiagalingam S

更新日期：2015-01-01 00:00:00

abstract：:Cancer numbers increasing, cases heterogeneity and the drug resistance emergence have pushed scientists to search for innovative solutions for patients and epimutations can be one. Methylated DNA, modified nucleosomes and noncoding RNAs are found in all cells, including tumor cells. They are intracellular actors but a...

journal_title：Epigenomics

authors： Duforestel M,Briand J,Bougras-Cartron G,Heymann D,Frenel JS,Vallette FM,Cartron PF

更新日期：2020-01-01 00:00:00

abstract：:Post-translational modifications of chromatin have become a 'booming' area of biomedical research. One particularly interesting modification that is important for eukaryotic gene expression is trimethylation of histone H3 lysine 4 (H3K4me3), which is almost exclusively associated with active promoters of RNA polymeras...

journal_title：Epigenomics

authors： Vermeulen M,Timmers HT

更新日期：2010-06-01 00:00:00

abstract：:The ability to culture pluripotent stem cells and direct their differentiation into specific cell types in vitro provides a valuable experimental system for modeling pluripotency, development and cellular differentiation. High-throughput profiling of the transcriptomes and epigenomes of pluripotent stem cells and thei...

journal_title：Epigenomics

authors： Thiagarajan RD,Morey R,Laurent LC

更新日期：2014-02-01 00:00:00

abstract：:Hematopoiesis is a regulated multistep process, whereby transcriptional and epigenetic events contribute to progenitor fate determination. miRNAs have emerged as key players in hematopoietic cell development, differentiation and malignant transformation. From embryonic development through to adult life, miRNAs coopera...

journal_title：Epigenomics

authors： Pagano F,De Marinis E,Grignani F,Nervi C

更新日期：2013-01-01 00:00:00

abstract：:Aim: To develop a method for estimating cell-specific effects in epigenomic association studies in the presence of cell type heterogeneity. Materials & methods: We utilized Monte Carlo Expectation-Maximization (MCEM) algorithm with Metropolis-Hastings sampler to reconstruct the 'missing' cell-specific methylations and...

journal_title：Epigenomics

authors： Feng YA,Guo Y,Pain L,Lathrop GM,Laprise C,Moffatt MF,Cookson WO,Liang L

更新日期：2020-12-22 00:00:00

abstract：AIM:In nondividing neurons examine the role of Gadd45b in active 5-methylcytosine (5MC) and 5-hydroxymethylcytosine (5HMC) removal at a gene promoter highly implicated in mental illnesses and cognition, Bdnf. MATERIALS & METHODS:Mouse primary cortical neuronal cultures with and without Gadd45b siRNA transfection were ...

journal_title：Epigenomics

authors： Gavin DP,Kusumo H,Sharma RP,Guizzetti M,Guidotti A,Pandey SC

更新日期：2015-01-01 00:00:00

abstract：:Histone deacetylase (HDAC)6 is a member of the class IIb HDAC family. This enzyme is zinc-dependent and mainly localized in the cytoplasm. HDAC6 is a unique isoenzyme with two functional catalytic domains and specific physiological roles. Indeed, HDAC6 deacetylates various substrates including α-tubulin and HSP90α, an...

journal_title：Epigenomics

authors： Seidel C,Schnekenburger M,Dicato M,Diederich M

更新日期：2015-01-01 00:00:00

abstract：OBJECTIVE:To study DNA methylation patterns of cortical pyramidal layers susceptible to late-onset Alzheimer's disease (LOAD) neurodegeneration. METHODS:Laser-assisted microdissection to select pyramidal layers' cells in frontal cortex of 32 human brains (18 LOAD) and Infinium DNA Methylation 450K analysis were perfor...

journal_title：Epigenomics

authors： Hernández HG,Sandoval-Hernández AG,Garrido-Gil P,Labandeira-Garcia JL,Zelaya MV,Bayon GF,Fernández AF,Fraga MF,Arboleda G,Arboleda H

更新日期：2018-11-01 00:00:00

abstract：:DNA modifications represent an integral part of the epigenome and they have a pivotal role in regulation of genome function. Despite the wide variety of analytical techniques that have been developed to detect DNA modifications, their investigation at the single-genome level is only beginning to emerge. In contrast to...

journal_title：Epigenomics

authors： Zillner K,Németh A

更新日期：2012-08-01 00:00:00

abstract：AIM:To identify differentially methylated probes (DMPs) and regions (DMRs) in relation to chronic obstructive pulmonary disease (COPD) and lung function traits. METHODS:We performed an epigenome-wide association study of COPD and spirometric parameters, including forced expiratory volume in 1 s (FEV1), forced vital ca...

journal_title：Epigenomics

authors： Lee MK,Hong Y,Kim SY,Kim WJ,London SJ

更新日期：2017-07-01 00:00:00

abstract：AIM:To characterize the genotypic and phenotypic extent of multilocus imprinting disturbances (MLID). MATERIALS & METHODS:We analyzed 37 patients with imprinting disorders (explorative cohort) for DNA methylation changes using the Infinium HumanMethylation450 BeadChip. For validation, three independent cohorts with im...

journal_title：Epigenomics

authors： Bens S,Kolarova J,Beygo J,Buiting K,Caliebe A,Eggermann T,Gillessen-Kaesbach G,Prawitt D,Thiele-Schmitz S,Begemann M,Enklaar T,Gutwein J,Haake A,Paul U,Richter J,Soellner L,Vater I,Monk D,Horsthemke B,Ammerpohl O,

更新日期：2016-06-01 00:00:00

abstract：:Aim: To dynamically analyze the differential m6A methylation during the progression and reversal of hepatic fibrosis. Materials & methods: We induced hepatic fibrosis in C57/BL6 mice by intraperitoneal injection of CCl4. The reversal model of hepatic fibrosis was established by stopping drug after continuous injection...

journal_title：Epigenomics

authors： Cui Z,Huang N,Liu L,Li X,Li G,Chen Y,Wu Q,Zhang J,Long S,Wang M,Sun F,Shi Y,Pan Q

更新日期：2020-10-01 00:00:00

abstract：AIM:To investigate epigenomic changes in pregnancy and early postpartum in women with and without type 2 diabetes. METHODS:Dimethylation of histones H3K4, H3K9, H3K27, H3K36 and H3K79 was measured in white blood cells of women at 30 weeks pregnancy, at 8-10 and 20 weeks postpartum and in never-pregnant women. RESULTS...

journal_title：Epigenomics

authors： Michalczyk AA,Janus ED,Judge A,Ebeling PR,Best JD,Ackland MJ,Asproloupos D,Dunbar JA,Ackland ML

更新日期：2018-04-01 00:00:00

abstract：:The E3 ubiquitin ligase UBE3A, also known as E6-AP, has a multitude of ascribed functions and targets relevant to human health and disease. Epigenetic regulation of the UBE3A gene by parentally imprinted noncoding transcription within human chromosome 15q11.2-q13.3 is responsible for the maternal-specific effects of 1...

journal_title：Epigenomics

authors： LaSalle JM,Reiter LT,Chamberlain SJ

更新日期：2015-10-01 00:00:00

abstract：:Aim: The hygiene hypothesis states that a lack of infection in early-life suppresses immune system development, and is linked to respiratory allergy (RA) and childhood acute lymphoblastic leukemia (ALL) risk. Little is known about underlying mechanisms, but DNA methylation is altered in RA and ALL, and in response to ...

journal_title：Epigenomics

authors： Langie SA,Timms JA,De Boever P,McKay JA

更新日期：2019-10-01 00:00:00

abstract：:Aim: To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus rat model, through investigating its effect at the epigenetic, mRNA and protein levels. Materials & methods: Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnet...

journal_title：Epigenomics

authors： Matboli M,Ibrahim D,Hasanin AH,Hassan MK,Habib EK,Bekhet MM,Afifi AM,Eissa S

更新日期：2021-01-07 00:00:00

abstract：AIM:We compared predictive modeling approaches to estimate placental methylation using cord blood methylation. MATERIALS & METHODS:We performed locus-specific methylation prediction using both linear regression and support vector machine models with 174 matched pairs of 450k arrays. RESULTS:At most CpG sites, both ap...

journal_title：Epigenomics

authors： De Carli MM,Baccarelli AA,Trevisi L,Pantic I,Brennan KJ,Hacker MR,Loudon H,Brunst KJ,Wright RO,Wright RJ,Just AC

更新日期：2017-03-01 00:00:00

abstract：:Currently, the incidence of colorectal cancer (CRC) is increasing across the world. The cancer stroma exerts an impact on the spread, invasion and chemoresistance of CRC. The tumor microenvironment involves a complex interaction between cancer cells and stromal cells, for example, cancer-associated fibroblasts (CAFs)....

journal_title：Epigenomics

authors： Savardashtaki A,Shabaninejad Z,Movahedpour A,Sahebnasagh R,Mirzaei H,Hamblin MR

更新日期：2019-11-01 00:00:00

abstract：:Imprinted genes are an epigenetically regulated class of genes that are preferentially expressed from one parental allele. A number of these genes are crucial for placental function and embryonic growth in mice and humans. Disruption of imprinted genes is also associated with several neurodevelopmental disorders, alth...

journal_title：Epigenomics

authors： Kernohan KD,Bérubé NG

更新日期：2010-12-01 00:00:00

abstract：AIM:Aberrant epigenetic events are important contributors to the pathogenesis of different types of cancers and dietary botanicals with epigenetic properties can influence early cancer development leading to cancer prevention effects. We sought to investigate potential combinatorial effects of bioactive dietary compone...

journal_title：Epigenomics

authors： Li Y,Buckhaults P,Cui X,Tollefsbol TO

更新日期：2016-08-01 00:00:00

abstract：:Fibrosis is the excess deposition of extracellular matrix components which occur in multiple organs and ultimately leads to organ failure. Long noncoding RNAs (lncRNAs) are a kind of noncoding RNAs longer than approximately 200 nucleotides with no protein-encoding capacity. A growing body of evidence suggests that lnc...

journal_title：Epigenomics

authors： Jiang X,Zhang F

更新日期：2017-09-01 00:00:00

abstract：AIM:To identify regions of aberrant DNA methylation in acute lymphoblastic leukemia (ALL) cells of different subtypes on a genome-wide scale. MATERIALS & METHODS:Whole-genome bisulfite sequencing (WGBS) was used to determine the DNA methylation levels in cells from four pediatric ALL patients of different subtypes. Th...

journal_title：Epigenomics

authors： Wahlberg P,Lundmark A,Nordlund J,Busche S,Raine A,Tandre K,Rönnblom L,Sinnett D,Forestier E,Pastinen T,Lönnerholm G,Syvänen AC

更新日期：2016-10-01 00:00:00

abstract：:Manipulation of chromatin, in which genomic DNA is packaged, is a fundamental requirement for all DNA-based metabolic processes in eukayotic cells. Histone variant incorporation, histone post-translational modifications, and ATP-dependent chromatin remodeling are three major strategies for chromatin manipulation, and ...

journal_title：Epigenomics

authors： Bao Y

更新日期：2011-06-01 00:00:00

abstract：:A number of bioactive dietary components are of particular interest in the field of epigenetics. Many of these compounds display anticancer properties and may play a role in cancer prevention. Numerous studies suggest that a number of nutritional compounds have epigenetic targets in cancer cells. Importantly, emerging...

journal_title：Epigenomics

authors： Hardy TM,Tollefsbol TO

更新日期：2011-08-01 00:00:00

abstract：:Aim: This study aims to examine the DNA methylation (DNAm) and expression patterns of genes associated with placental angiogenesis in preeclampsia. Materials & methods: DNAm and expression were examined in normotensive (n = 100) and preeclampsia (n = 100) women using pyrosequencing and quantitative real-time PCR respe...

journal_title：Epigenomics

authors： M Dave K,Kaur L,Randhir KN,Mehendale SS,Sundrani DP,Chandak GR,Joshi SR

更新日期：2021-01-20 00:00:00