A molecular imprinted SPR biosensor for sensitive determination of citrinin in red yeast rice.

Abstract:

:A novel and sensitive molecular imprinted surface plasmon resonance (SPR) biosensor was developed for selective determination of citrinin (CIT) in red yeast rice. Firstly, the gold surface of SPR chip was modified with allyl mercaptane. Then, CIT-imprinted poly(2-hydroxyethyl methacrylate-methacryloylamidoglutamic acid) (p(HEMA-MAGA)) film was generated on the gold surface modified with allyl mercaptane. The unmodified and imprinted surfaces were characterized by Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM) and contact angle measurements. The linearity range and the detection limit were obtained as 0.005-1.0 ng/mL and 0.0017 ng/mL, respectively. The SPR biosensor was applied to determination of CIT in red yeast rice sample.

journal_name

Food Chem

journal_title

Food chemistry

authors

Atar N,Eren T,Yola ML

doi

10.1016/j.foodchem.2015.03.065

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

7-11

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(15)00437-9

journal_volume

184

pub_type

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