当前位置： SCI文献检索 > JOURNAL OF ETHNOPHARMACOLOGY期刊下所有文献 > The traditional drug Gongjin-Dan ameliorates chronic fatigue in a forced-stress mouse exercise model.

The traditional drug Gongjin-Dan ameliorates chronic fatigue in a forced-stress mouse exercise model.

Abstract：

ETHNOPHARMACOLOGICAL RELEVANCE:Gongjin-Dan is a representative traditional Oriental medicine herbal drug that has been used to treat chronic fatigue symptoms for several hundred years. We evaluated the anti-fatigue effects of Gongjin-Dan and the underlying mechanisms in a chronic forced exercise mouse model. METHODS AND MATERIALS:Balb/C male mice underwent an extreme treadmill-based running stress (1-h, 5 days/week), and daily oral administration of distilled water, Gongjin-Dan (100, 200, or 400 mg/kg), or ascorbic acid (100 mg/kg) for 28 days. The anti-fatigue effects of Gongjin-Dan were evaluated with behavioral tests (exercise tolerance and swimming tests), and the corresponding mechanisms were investigated based on oxidative stress and inflammatory cytokine and stress hormone levels in skeletal muscle, sera, and brain tissue. RESULTS:Gongjin-Dan significantly increased exercise tolerance and latency times but reduced the number of electric shocks and immobilization time on the treadmill running and swimming tests, compared with the control group. Gongjin-Dan also significantly ameliorated alterations in oxidative stress-related biomarkers (reactive oxygen species and malondialdehyde), inflammatory cytokines (tumor necrosis factor-α, interleukin-1 beta, interleukin-6, and interferon-γ) and glycogen and L-lactate levels in skeletal muscle, compared with those in the control group. Moreover, Gongjin-Dan considerably normalized the forced running stress-induced changes in serum corticosterone and adrenaline levels, as well as brain serotonin level. These antioxidant and anti-stress effects of Gongjin-Dan were supported by the results of Western blotting (4-hydroxynonenal and heme oxygenase-1) and the gene expression levels (serotonin receptor and serotonin transporter). CONCLUSION:These results support the clinical relevance of Gongjin-Dan regarding anti-chronic fatigue properties. The underlying mechanisms involve attenuation of oxidative and inflammatory reactions in muscle and regulation of the stress response through the hypothalmo-pituitary-adrenal axis.

journal_name

J Ethnopharmacol

journal_title

Journal of ethnopharmacology

authors

Hong SS,Lee JY,Lee JS,Lee HW,Kim HG,Lee SK,Park BK,Son CG

doi

10.1016/j.jep.2015.04.001

subject

Has Abstract

pub_date

2015-06-20 00:00:00

pages

268-78

eissn

0378-8741

issn

1872-7573

pii

S0378-8741(15)00242-1

journal_volume

168

pub_type

杂志文章

在线工具

The traditional drug Gongjin-Dan ameliorates chronic fatigue in a forced-stress mouse exercise model.