Abstract:
:The correct functionality (sensitivity and receptivity) of endometrial tissue is regulated by paracrine and endocrine pathways that activate several mediators or metabolic pathways and gene cascades. This study aimed to investigate the influence of E2 and P4 on progesterone receptor (PGR) and progesterone receptor membrane component 1 (PGRMC1) protein expression in porcine luminal epithelial cells and their influence on the proliferation of these cells in real-time. Surface uterine luminal epithelial cells were removed using sterile surgical blades from uterine horns of ten crossbred anestrus gilts. Following treatment with collagenase I, cells were separated and transferred into 48-well E-Plates for use in a realtime cell analyzer (RTCA). The luminal epithelial cells were cultured in vitro (IVC) in standard DMEM cell culture medium and incubated with E2 (10 pg/ml, 40 pg/ml, 500 pg/ml) and P4 (10 ng/ml, 40 ng/ ml, 500 ng/ml). The cell proliferation index was analyzed after 0-240 h, 0-120 h, 120-240 h. After using the RTCA analysis we found increased proliferation of luminal epithelial cells after treatment of low doses of P4 (10 and 40 ng/ml), (P < 0.001). Higher doses of P4 led to decrease of proliferation (P < 0.001). Conversely, higher doses of E2 (500 pg/ml) increased the proliferation index as compared to low doses (10 pg/ml) and control (P < 0.001). Confocal microscopic observations revealed that higher concentrations of E2 upregulate the expression of both PGR and PGRMC1. Additionally, P4 used in lower concentrations stimulated the expression of these receptors, too. Our study presents a new influence of E2 and P4 on the expression of PGR and PGRMC1 and on the real-time proliferation of porcine luminal epithelial cells. The relationship between PGR or PGRMC1 expression and the proliferation of luminal epithelial cells may be influenced (up- or down regulated) by E2 or P4 in a steroid type- and dose-dependent manner.
journal_name
J Biol Regul Homeost Agentsjournal_title
Journal of biological regulators and homeostatic agentsauthors
Kempisty B,Wojtanowicz-Markiewicz K,Ziółkowska A,Budna J,Ciesiółka S,Piotrowska H,Bryja A,Antosik P,Bukowska D,Wollenhaupt K,Bruska M,Brüssow KP,Nowicki M,Zabel Msubject
Has Abstractpub_date
2015-01-01 00:00:00pages
39-50issue
1eissn
0393-974Xissn
1724-6083pii
5journal_volume
29pub_type
杂志文章abstract::Allergic rhinitis (AR) is caused by an IgE-mediated inflammatory reaction. Non-allergic rhinitis (NAR) is characterized by a non-IgE-mediated pathogenesis. Frequently, patients have the two disorders associated: such as mixed rhinitis (MR). Hyaluronic acid (HA) is a fundamental component of the human connective tissue...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2016-01-01 00:00:00
abstract::The contribution of plasma prekallikrein (PK) to vascular remodeling is becoming increasingly recognized. Plasma PK is activated when the zymogen PK is digested to an active enzyme by activated factor XII (FXII). Here, we present our findings that vascular smooth muscle cells (VSMC) activate plasma PK in the absence o...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 社论
doi:
更新日期:2014-10-01 00:00:00
abstract::Long noncoding RNA (lncRNA) LINC00473 has been reported to be involved in the regulation of several human cancers. However, the regulatory mechanism of LINC00473 is still unknown in lung adenocarcinoma. In this study, RT-qPCR was used to measure the expression of LINC00473, miR-1294 and ROBO1. The functional mechanism...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:10.23812/20-75A
更新日期:2020-04-29 00:00:00
abstract::Allergen-specific immunoglobulin E (IgE) reactions lead to acute degranulation of mast cells and basophils and release of stored mediators, particularly tryptase and histamine, which can be measured in vitro after reactions. The aim of this study was to investigate the utility of serum tryptase and plasma histamine du...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2015-04-01 00:00:00
abstract::Acute abdominal aortic thrombosis is a rare and potential fatal event, which occurs in adult subjects. We present the case of a 72-year-old-man, who referred to the emergency Department of our hospital because of persistent severe abdominal and perineal pain. Doppler ultrasounds and computerized tomography angiography...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
doi:
更新日期:2013-04-01 00:00:00
abstract::Acute lung injury (ALI) is a disease with high incidence and no effective therapeutic treatments. miR- 145-5p has been reported to be aberrantly expressed in lung injury tissues, suggesting a potential role in the progression and development of ALI. To validate this hypothesis and explore the underlying mechanism, a m...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2019-07-01 00:00:00
abstract::Three main types of inflammatory Non-Allergic Rhinitis (NAR) have been defined: NAR infiltrated by eosinophils (NARES), by mast cells (NARMA), and by neutrophils (NARNE). In the absence of studies that investigated the Quality of Life (QoL) in NAR, the present work is aimed at evaluating the Quality of Life of patient...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::First, we review basic concepts of Tissue Engineering, that is, how the tensegrity is able to modulate the cell behavior. Then, we review our experimental results regarding the bone tissue engineering via biomaterials and bioreactors. ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
更新日期:2018-11-01 00:00:00
abstract::Direct anterior approach to the hip allows perfect exposure of the acetabulum and an easy proximal and medial extension that makes it eligible for isolate acetabular cup revision although it is seldom used and there are only few published studies. On 23 consecutive acetabular revision (16 cases Paprosky grade 1 or 2, ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
更新日期:2016-10-01 00:00:00
abstract::Oral mucosa is constantly under the influence of various internal and external stimuli, therefore it is interested by a range of developmental disorders, irritation, inflammation, and neoplastic conditions. Traumatic fibroma is one of these lesions. The aim of this work is to obtain an ultrasound characterization of a...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2017-04-01 00:00:00
abstract::Diabetic nephropathy (DN) is a severe and life-threatening complication of long-standing diabetes. As one of the main causes of end-stage renal disease, the prevention and treatment of DN in early stage, and the slowing down of DN progression are of utmost importance and are topics of several ongoing research studies....
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::This study aims to analyze the clinical characteristics of breast invasive ductal carcinoma (BIDC) in patients with different molecular subtypes and identify possible correlation to prognosis. miR- 10b expression level was detected using real-time quantitative polymerase chain reaction (RT-PCR). Tissue sections were c...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2018-05-01 00:00:00
abstract:: ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
doi:
更新日期:2019-01-01 00:00:00
abstract::The similarity between humans and pigs, when it comes to tissue morphology, makes Sus scrofa not only a good research model, but also a potential source of cells for tissue engineering. Cell samples obtained from the pig donor, could be influenced in vitro, in order to become a source of tissue material for xenotransp...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2018-09-01 00:00:00
abstract:OBJECTIVES:To evaluate the effect of autologous platelet and plasma adhesives (APA) on postoperative drainage and soft-tissue fibrosis following neck dissections. DESIGN:This was a blinded comparative prospective cohort study done as two parts: part one evaluated early post-surgical outcomes and part two evaluated lat...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2012-04-01 00:00:00
abstract::Various hematopoietic malignant disorders have been shown to bear chromosome abnormalities of the 11q23 band, which can be rearranged with many different chromosomal regions in a wide variety of different leukemia subtypes. Several laboratories have identified a trithorax-related gene that is involved in most of the 1...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
更新日期:1998-10-01 00:00:00
abstract::The objective of this study was to compare the efficacy of supportive periodontal therapy (i.e. scaling and root planning, SRP) alone, versus a chemical device silica dioxide (SiO2) colloidal solutions (SDCS) used in association with SRP in the treatment of chronic periodontitis in adult patients. A total of 20 patien...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2019-12-10 00:00:00
abstract::Many studies demonstrated that human adult cardiac progenitor cells in the form of cardiospheres (CSps) could represent a powerful candidate for cardiac cell therapy. To achieve the clinical translation of this biotechnological product, the development of well-defined culture conditions is required to optimize their p...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2011-04-01 00:00:00
abstract::Ahead of Print article withdrawn by publisher. ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 社论,收录出版
doi:10.23812/20-269-E-4
更新日期:2020-07-16 00:00:00
abstract::Vertebral fusion is performed in order to stabilize the spine in the presence of degenerative, traumatic or oncological pathologies that alter its stability. The autologous bone, harvested from the patient’s iliac crest or from the lamina during surgery, is still considered the “gold standard” for spine fusion due to ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2017-10-01 00:00:00
abstract::The lack of distinguishing characteristics between lymphoid blastic crisis (BC) of Philadelphia (Ph)+ chronic myeloid leukemia (CML) and Ph+ acute lymphoblastic leukemia (ALL) remains an exciting dilemma. Indeed, the genetic defect of approximately half of Ph+ ALL patients is identical to that identified in CML. Here ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::IL-32, a newly-discovered proinflammatory cytokine that activates the p38MAPK and NF-kappaB pathways, is an important player in innate and adaptive immune response. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the productio...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 社论,评审
doi:
更新日期:2009-07-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2014-10-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2005-07-01 00:00:00
abstract::Hepatitis C virus (HCV) is a common transmissible agent responsible for a significant proportion of chronic liver disease, cirrhosis and hepatocellular carcinoma worldwide. The natural history of acute HCV infection is characterized by a high rate of progression to persistent infection. The resulting chronic liver dis...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
更新日期:2003-04-01 00:00:00
abstract::A successful Total Knee Arthroplasty (TKA) requires stability, but rarely in primary TKA, a prosthesis with more constraint than a posterior-stabilizer (PS) is necessary. In patients with severe varus/valgus deformities with incompetent collateral ligaments or in knees that cannot be adequately balanced after ligament...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2017-10-01 00:00:00
abstract::To study the cytology profile of cervical smears and the respective prevalence and incidence of certain cervico-vaginal infections detectable by routine Pap smear cytology in Lebanese women from 2002 to 2006. Pap smear cytology results were compiled from the archives of the Institut National de Pathologie for the peri...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 临床试验,信件,多中心研究
doi:
更新日期:2011-07-01 00:00:00
abstract::The purpose of this study was to concomitantly determine oxidative DNAdamage and bone resorption following a rapid body mass reduction in association with energy restriction and exercise training, considering 17β-estradiol level, in female collegiate judokas. Eighteen nationally ranked university female judokas were e...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2018-07-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2017-10-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2017-07-01 00:00:00