Small molecule ice recrystallization inhibitors enable freezing of human red blood cells with reduced glycerol concentrations.

Abstract:

:In North America, red blood cells (RBCs) are cryopreserved in a clinical setting using high glycerol concentrations (40% w/v) with slow cooling rates (~1°C/min) prior to storage at -80°C, while European protocols use reduced glycerol concentrations with rapid freezing rates. After thawing and prior to transfusion, glycerol must be removed to avoid intravascular hemolysis. This is a time consuming process requiring specialized equipment. Small molecule ice recrystallization inhibitors (IRIs) such as β-PMP-Glc and β-pBrPh-Glc have the ability to prevent ice recrystallization, a process that contributes to cellular injury and decreased cell viability after cryopreservation. Herein, we report that addition of 110 mM β-PMP-Glc or 30 mM β-pBrPh-Glc to a 15% glycerol solution increases post-thaw RBC integrity by 30-50% using slow cooling rates and emphasize the potential of small molecule IRIs for the preservation of cells.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Capicciotti CJ,Kurach JD,Turner TR,Mancini RS,Acker JP,Ben RN

doi

10.1038/srep09692

subject

Has Abstract

pub_date

2015-04-08 00:00:00

pages

9692

issn

2045-2322

pii

srep09692

journal_volume

5

pub_type

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