Enzymatic transesterification of waste vegetable oil to produce biodiesel.

Abstract:

:An experimental study on enzymatic transesterification was performed to produce biodiesel from waste vegetable oils. Lipase from Pseudomonas cepacia was covalently immobilized on a epoxy-acrylic resin support. The immobilized enzyme exhibited high catalytic specific surface and allowed an easy recovery, regeneration and reutilisation of biocatalyst. Waste vegetable oils - such as frying oils, considered not competitive with food applications and wastes to be treated - were used as a source of glycerides. Ethanol was used as a short chain alcohol and was added in three steps with the aim to reduce its inhibitory effect on lipase activity. The effect of biocatalyst/substrate feed mass ratios and the waste oil quality have been investigated in order to estimate the process performances. Biocatalyst recovery and reuse have been also studied with the aim to verify the stability of the biocatalyst for its application in industrial scale.

journal_name

Ecotoxicol Environ Saf

authors

Lopresto CG,Naccarato S,Albo L,De Paola MG,Chakraborty S,Curcio S,Calabrò V

doi

10.1016/j.ecoenv.2015.03.028

subject

Has Abstract

pub_date

2015-11-01 00:00:00

pages

229-35

eissn

0147-6513

issn

1090-2414

pii

S0147-6513(15)00127-X

journal_volume

121

pub_type

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