Characterisation of epitopes on human tissue plasminogen activator recognised by a group of monoclonal antibodies.

Abstract:

:Seven mouse monoclonal antibodies have been produced against human melanoma tissue plasminogen activator (t-PA). They were specifically bound to 125I t-PA but not 125I urokinase (u-PA) and inhibited t-PA, but not u-PA, activity in plasminogen-rich 125I fibrin wells. Three of the antibodies directly inhibited the amidolytic activity of t-PA and the two most effective also bound near the active site histidine residue as determined by competition experiments using active site blocking agents. Several antibodies interfered with the fibrin binding properties of t-PA. One antibody neither interacted with the active site nor inhibited fibrin binding but still effectively quenched t-PA activity in fibrin wells suggesting that it masks another region of the molecule necessary for effective biological activity.

journal_name

Thromb Haemost

authors

MacGregor IR,Micklem LR,James K,Pepper DS

subject

Has Abstract

pub_date

1985-02-18 00:00:00

pages

45-50

issue

1

eissn

0340-6245

issn

2567-689X

journal_volume

53

pub_type

杂志文章
  • Activation and inactivation of human protein C by plasmin.

    abstract::Transient procoagulant states resulting in failure of recanalization or rethrombosis of the reperfused artery during thrombolytic therapy might be due to an inhibitory effect of plasmin on the anticoagulant properties of protein C. We therefore studied the effect of plasmin on protein C (PC) and activated protein C (A...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Váradi K,Philapitsch A,Santa T,Schwarz HP

    更新日期:1994-05-01 00:00:00

  • Familial coagulation factor V deficiency caused by a novel 4 base pair insertion in the factor V gene: factor V Stanford.

    abstract::An index patient with pseudohomozygosity for factor V Leiden was identified. Each of his two children inherited a different paternal factor V allele; a daughter was heterozygous for factor V Leiden, with 100% factor V activity, and a son was heterozygous for factor V deficiency, with 50% factor V activity. Genomic DNA...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Zehnder JL,Hiraki DD,Jones CD,Gross N,Grumet FC

    更新日期:1999-09-01 00:00:00

  • Hyperglycaemia suppresses microRNA expression in platelets to increase P2RY12 and SELP levels in type 2 diabetes mellitus.

    abstract::Megakaryocyte (MK)-derived miRNAs have been detected in platelets. Here, we analysed the expression of platelet and circulating miR-223, miR-26b, miR-126 and miR-140 that might be altered with their target mRNAs in type 2 diabetes mellitus (DM2). MiRNAs were isolated from leukocyte-depleted platelets and plasma sample...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1160/TH16-04-0322

    authors: Fejes Z,Póliska S,Czimmerer Z,Káplár M,Penyige A,Gál Szabó G,Beke Debreceni I,Kunapuli SP,Kappelmayer J,Nagy B Jr

    更新日期:2017-02-28 00:00:00

  • Effector functions of pathogenic Yersinia species.

    abstract::Pathogenic species of the genus Yersinia suppress and reorient the immune system to infect lymphatic tissues, inner organs and at times also the vasculature. For this purpose yersiniae employ a type III secretion system to translocate effector proteins (Yersinia outer proteins; Yops) into immune cells. Yops often exer...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,评审

    doi:

    authors: Aepfelbacher M,Trasak C,Ruckdeschel K

    更新日期:2007-09-01 00:00:00

  • Pharmacokinetics and Pharmacodynamics of Emicizumab in Persons with Hemophilia A with Factor VIII Inhibitors: HAVEN 1 Study.

    abstract::Emicizumab, a bispecific monoclonal antibody, bridges activated factor IX (FIXa) and FX, replacing the function of missing FVIIIa to restore effective hemostasis in persons with hemophilia A (PwHA). Here we assess pharmacokinetic (PK) and pharmacodynamic (PD) biomarkers in PwHA with FVIII inhibitors in the Phase III H...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1055/s-0040-1717114

    authors: Schmitt C,Adamkewicz JI,Xu J,Petry C,Catalani O,Young G,Negrier C,Callaghan MU,Levy GG

    更新日期:2020-10-21 00:00:00

  • Low-dose warfarin in atrial fibrillation leads to more thromboembolic events without reducing major bleeding when compared to adjusted-dose--a meta-analysis.

    abstract::The use of warfarin with a range INR of 2.0-3.0 is recommended in prevention of stroke for nonvalvular atrial fibrillation (AF) patients, in particular those older than 75 years. The risk of bleeding that is associated with this range of INR has led to evaluate lower ranges (low-dose or fixed minidose) in terms of ris...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,meta分析

    doi:10.1160/TH03-08-0541

    authors: Perret-Guillaume C,Wahl DG

    更新日期:2004-02-01 00:00:00

  • Prolonged exposure to high insulin impairs the endothelial PI3-kinase/Akt/nitric oxide signalling.

    abstract::Hyperinsulinemia predicts future cardiovascular events, but may also contribute to atherosclerosis. We therefore studied the consequences of prolonged insulin treatment of human umbilical vein endothelial cells (HUVEC) on the phosphatidylinositol-3'-kinase(PI3K)/Akt/nitric oxide(NO)-dependent insulin signaling, togeth...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Madonna R,De Caterina R

    更新日期:2009-02-01 00:00:00

  • Heparin induced thrombocytopenia: a prospective study.

    abstract::Thrombocytopenia occurred in 11.6% of 43 patients prospectively randomized to receive either bovine lung or porcine mucosal heparin. Four patients received bovine heparin and developed mild to moderate thrombocytopenia after eight to twelve days which resolved when therapy was stopped in one to six days. One patient r...

    journal_title:Thrombosis and haemostasis

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Ansell J,Slepchuk N Jr,Kumar R,Lopez A,Southard L,Deykin D

    更新日期:1980-02-29 00:00:00

  • Intraoperative fresh-frozen plasma versus human albumin in craniofacial surgery--a pilot study comparing coagulation profiles in infants younger than 12 months.

    abstract::The transfusion of fresh-frozen plasma (FFP) is suggested to minimize dilution coagulopathy when applied instead of colloids during paediatric craniofacial surgery (pCFS). We prospectively compared plasmatic haemostaseologic function between volume replacement with FFPs versus human albumin (HA) in a pilot study. Thir...

    journal_title:Thrombosis and haemostasis

    pub_type: 临床试验,杂志文章

    doi:

    authors: Hildebrandt B,Machotta A,Riess H,Kerner S,Ahlers O,Haberl H,Dörken B,Kerner T

    更新日期:2007-07-01 00:00:00

  • European Concerted Action on Anticoagulation (ECAA)-the multicentre calibration of rabbit and human ECAA reference thromboplastins. Steering Committee.

    abstract::Two candidate ECAA reference preparations have been calibrated in a multicentre exercise at 14 representative national laboratories to provide reference thromboplastin for a large field study in 16 European countries. Two preparations were required because of the established differences in ISI arising from the two mai...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,多中心研究

    doi:

    authors: Poller L,Barrowcliffe TW,van den Besselaar AM,Jespersen J,Tripodi A,Houghton D

    更新日期:1996-12-01 00:00:00

  • Increase in plasma concentration of plasminogen activator inhibitor, fibrinogen, von Willebrand factor, factor VIII:C and in erythrocyte sedimentation rate with age.

    abstract::Elderly patients have previously been shown to have an increased plasma concentration of tissue plasminogen activator (t-PA) antigen (t-PA Ag). Since the concentration of t-PA Ag depends on both free t-PA and t-PA complexed with inhibitors, mainly plasminogen activator inhibitor (PA inhibitor), we have investigated th...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Aillaud MF,Pignol F,Alessi MC,Harle JR,Escande M,Mongin M,Juhan-Vague I

    更新日期:1986-06-30 00:00:00

  • Growth hormone deficiency impairs blood clotting and reduces factor VII coagulant activity in rat.

    abstract::To investigate pituitary effects on the vitamin K-dependent coagulation factors, female rats were hypophysectomized (hypox) and treated with growth hormone (GH), cortisone, thyroxine, vitamin K, or saline. After 11 days of treatment, the prothrombin time, platelet count, and factors II, VII, IX, and X were determined....

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Sävendahl LS,Grankvist K,Engström KG

    更新日期:1995-04-01 00:00:00

  • High molecular weight forms of antithrombin III complexes in blood.

    abstract::A double antibody competition radioimmunoassay was developed that allowed to detect specifically as little as 15 ng antithrombin III (AT III) per ml of the assayed material. In normal plasma examined by this assay, AT III concentration averaged 199 +/- 21 micrograms/ml. Complexes of AT III with thrombin or factor Xa c...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Marciniak E,Gora-Maslak G

    更新日期:1983-02-28 00:00:00

  • Evaluation of frozen plasma calibrants for enhanced standardization of the international normalized ratio (INR): a multi-center study.

    abstract::Local ISI calibration has been proposed to improve INR accuracy and inter-laboratory precision. We evaluated the affect of local PT calibration on INR precision and accuracy using six levels of frozen plasma calibrants prepared and pooled from normal donors and patients stabilized on sodium warfarin (coumarin) based o...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Adcock DM,Johnston M

    更新日期:2002-01-01 00:00:00

  • On the role of C1-inhibitor as inhibitor of tissue-type plasminogen activator in human plasma.

    abstract::An enzyme immuno assay was developed to measure complexes of tissue-type plasminogen activator (t-PA) with C1-inhibitor in order to study the role of C1-inhibitor as an inhibitor of t-PA in plasma. In vitro experiments with melanoma and recombinant t-PA learned that purified C1-inhibitor reacts with both single chain ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Huisman LG,van Griensven JM,Kluft C

    更新日期:1995-03-01 00:00:00

  • Effect of platelet antiserum on the precipitation of soluble fibrin by endotoxin.

    abstract::Rabbits were injected with a platelet antiserum to examine the role of thrombocytopenia of the precipitation of soluble fibrin by endotoxin.dotoxin. Platelet antiserum removed more than 98% of the circulating platelets, and the resulting thrombocytopenia with platelet counts below 10,000 per mul persisted during the e...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Müller-Berghaus G,Kramer W

    更新日期:1976-02-29 00:00:00

  • Ouabain enhances basal and stimulus-induced cytoplasmic calcium concentrations in platelets.

    abstract::Incubation of platelet-rich plasma (PRP) with ouabain, an inhibitor of sodium/potassium ATPase (Na+/K+ ATPase), induced a significant rise in basal platelet intracellular calcium concentration [( Ca2+]i) when measured using fura 2. Ouabain induced an enhanced aggregation response to low doses of collagen in both PRP a...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Lees AD,Wilson J,Orchard CH,Orchard MA

    更新日期:1989-11-24 00:00:00

  • Antithrombotic activity of a novel C-terminal hirudin analog in experimental animals.

    abstract::A synthetic hirudin55-65 C-terminal fragment analog was evaluated for anticoagulant activity and in models of experimental thrombosis in mice and rats. Intravenous injection caused dose-related inhibition of thrombin and anticoagulation in rat blood samples, protection from thromboembolism in mice and inhibition of st...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Broersma RJ,Kutcher LW,Heminger EF,Krstenansky JL,Marshall FN

    更新日期:1991-04-08 00:00:00

  • Aggregation of human platelets by acidic mucopolysaccharide extracted from Stichopus japonicus Selenka.

    abstract::The acidic mucopolysaccharide extracted from sea cucumber (Stichopus japonicus Selenka) (SJAMP) has been shown to cause platelets to aggregate. Using citrated platelet-rich plasma (PRP), washed platelets and formaldehyde-fixed platelets from humans, we investigated the effects of platelet inhibitors and various plasma...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Li JZ,Lian EC

    更新日期:1988-06-16 00:00:00

  • Role of gender and genetic variance in plasminogen activator inhibitor-1 secretion from human adipose tissue.

    abstract::Gender and the 4G/5G polymorphism in the plasminogen activator inhibitor 1 (PAI-1) gene are believed to play a role in the regulation of plasma PAI-1 activity. Adipose tissue has been found to be an important source of PAI-1. The possible influence of gender and the 4G/5G polymorphism in the PAI-1 gene on PAI-1 secret...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: van Harmelen V,Wahrenberg H,Eriksson P,Arner P

    更新日期:2000-02-01 00:00:00

  • A randomized trial of initial warfarin dosing based on simple clinical criteria.

    abstract::Warfarin induction is accomplished by titrating dosage to coagulation test results. Algorithms can guide this process but not identify the starting dose. We hypothesized that an initial warfarin dose approximating the maintenance value would safely enhance rapidity of induction. In a randomized trial we compared a fix...

    journal_title:Thrombosis and haemostasis

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:

    authors: Shine D,Patel J,Kumar J,Malik A,Jaeger J,Maida M,Ord L,Burrows G

    更新日期:2003-02-01 00:00:00

  • Gene expression in atherogenesis.

    abstract::It is conceivable that the extent and spatio-temperal expression of dozens or even a few hundred genes are significantly altered during the development and progression of atherosclerosis as compared to normal circumstances. Differential gene expression in vascular cells and in blood cells, due to gene-gene and gene-en...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,评审

    doi:

    authors: Monajemi H,Arkenbout EK,Pannekoek H

    更新日期:2001-07-01 00:00:00

  • Developmental haemostasis. Impact for clinical haemostasis laboratories.

    abstract::Developmental haemostasis is a concept, now universally accepted, introduced by Andrew et al. in the late 1980's. However, coagulation analysers and reagents have changed significantly over the past 15 years. Coagulation testing is known to be sensitive to changes in individual reagents and analysers. We hypothesised ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1160/TH05-01-0047

    authors: Monagle P,Barnes C,Ignjatovic V,Furmedge J,Newall F,Chan A,De Rosa L,Hamilton S,Ragg P,Robinson S,Auldist A,Crock C,Roy N,Rowlands S

    更新日期:2006-02-01 00:00:00

  • Evaluation of haemostatic molecular markers for diagnosis of disseminated intravascular coagulation in patients with infections.

    abstract::Early treatment of disseminated intravascular coagulation (DIC) is recommended but global coagulation tests used in authorized DIC criteria are not sensitive for diagnosis of early-phase DIC. We examined the plasma levels of thrombin-antithrombin complex (TAT), plasmin-plasmin inhibitor complex (PPIC) and D-dimer in p...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,多中心研究

    doi:10.1160/TH05-04-0286

    authors: Asakura H,Wada H,Okamoto K,Iba T,Uchiyama T,Eguchi Y,Kawasugi K,Koga S,Mayumi T,Koike K,Gando S

    更新日期:2006-02-01 00:00:00

  • Khorana score and histotype predicts incidence of early venous thromboembolism in non-Hodgkin lymphomas. A pooled-data analysis of 12 clinical trials of Fondazione Italiana Linfomi (FIL).

    abstract::Current data suggests that the risk of venous thromboembolism (VTE) in patients with non-Hodgkin lymphoma (NHL) is comparable to that observed in patients with solid tumours, although more robust confirmatory analyses are required. With that in mind, we investigated the occurrence of VTE in a pooled analysis of 12 "Fo...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1160/TH16-11-0895

    authors: Santi RM,Ceccarelli M,Bernocco E,Monagheddu C,Evangelista A,Valeri F,Monaco F,Vitolo U,Cortelazzo S,Cabras MG,Spina M,Baldini L,Boccomini C,Chiappella A,Bari A,Luminari S,Visco C,Calabrese M,Limberti G,Levis A,Con

    更新日期:2017-04-27 00:00:00

  • Functional consequences of mutations in amino acid residues that stabilize calcium binding to the first epidermal growth factor homology domain of human protein C.

    abstract::Charge-to-alanine mutations of three amino acid residues, viz, D46, D48, and D/Hya71, which are known to be important in stabilizing Ca2+ binding to epidermal growth factor (EGF) domains of vitamin K-dependent blood coagulation proteins, have been engineered into recombinant human protein C (r-PC). The resulting varia...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Geng JP,Cheng CH,Castellino FJ

    更新日期:1996-11-01 00:00:00

  • Changes in von Willebrand factor-cleaving protease (ADAMTS-13) in patients with aortic stenosis undergoing valve replacement or balloon valvuloplasty.

    abstract::It was the objective of this study to determine whether reduced cleavage of von Willebrand factor (VWF) multimers following aortic valve replacement (AVR) is a consequence of reduced shear stress or postoperative changes in VWF cleavage protease (ADAMTS-13) activity. Aortic stenosis (AS) may be complicated by acquired...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1160/TH11-12-0803

    authors: Bander J,Elmariah S,Aledort LM,Dlott J,Stelzer P,Halperin JL,Kini AS,Sharma SK

    更新日期:2012-07-01 00:00:00

  • Extracellular regulation of TGF-beta activity in wound repair: growth factor latency as a sensor mechanism for injury.

    abstract::The transforming growth factor-beta (TGFbeta) family of growth factors are major regulators of wound repair, scar formation, and fibrosis. One of the prominent features of TGFbeta biology is the fact that this growth factor is secreted as a latent precursor, which may be directed to and stored at specific sites in the...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,评审

    doi:10.1160/TH04-05-0324

    authors: Brunner G,Blakytny R

    更新日期:2004-08-01 00:00:00

  • Stroke and Thromboembolism in Warfarin-Treated Patients with Atrial Fibrillation: Comparing the CHA2DS2-VASc and GARFIELD-AF Risk Scores.

    abstract:BACKGROUND: Evaluation of thromboembolic risk is essential in anticoagulated atrial fibrillation (AF) patients. The CHA2DS2-VASc score is largely validated and recommended by most guidelines. The GARFIELD-AF Stroke score has been proposed as an alternative risk score. METHODS: We analyzed warfarin-treated patients fro...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1055/a-1333-4448

    authors: Proietti M,Rivera-Caravaca JM,Esteve-Pastor MA,Marín F,Lip GYH

    更新日期:2020-12-09 00:00:00

  • Oxidative stress and thromboxane-dependent platelet activation in inflammatory bowel disease: effects of anti-TNF-α treatment.

    abstract::Patients with inflammatory bowel disease (IBD) are at higher risk of venous thromboembolism and coronary artery disease despite having a lower burden of traditional risk factors. Platelets from IBD patients release more soluble CD40 ligand (CD40L), and this has been implicated in IBD platelet hyper-activation. We here...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1160/TH16-02-0167

    authors: Di Sabatino A,Santilli F,Guerci M,Simeone P,Ardizzone S,Massari A,Giuffrida P,Tripaldi R,Malara A,Liani R,Gurini E,Aronico N,Balduini A,Corazza GR,Davì G

    更新日期:2016-08-30 00:00:00