Abstract：
:MyoD and NeuroD2, master regulators of myogenesis and neurogenesis, bind to a "shared" E-box sequence (CAGCTG) and a "private" sequence (CAGGTG or CAGATG, respectively). To determine whether private-site recognition is sufficient to confer lineage specification, we generated a MyoD mutant with the DNA-binding specificity of NeuroD2. This chimeric mutant gained binding to NeuroD2 private sites but maintained binding to a subset of MyoD-specific sites, activating part of both the muscle and neuronal programs. Sequence analysis revealed an enrichment for PBX/MEIS motifs at the subset of MyoD-specific sites bound by the chimera, and point mutations that prevent MyoD interaction with PBX/MEIS converted the chimera to a pure neurogenic factor. Therefore, redirecting MyoD binding from MyoD private sites to NeuroD2 private sites, despite preserved binding to the MyoD/NeuroD2 shared sites, is sufficient to change MyoD from a master regulator of myogenesis to a master regulator of neurogenesis.
journal_name
Cell Repjournal_title
Cell reportsauthors
Fong AP,Yao Z,Zhong JW,Johnson NM,Farr GH 3rd,Maves L,Tapscott SJdoi
10.1016/j.celrep.2015.02.055subject
Has Abstractpub_date
2015-03-31 00:00:00pages
1937-46issue
12issn
2211-1247pii
S2211-1247(15)00232-6journal_volume
10pub_type
杂志文章相关文献
Cell Reports文献大全abstract：:Eukaryotic DNA replication licensing is a prerequisite for, and plays a role in, regulating genome duplication that occurs exactly once per cell cycle. ORC (origin recognition complex) binds to and marks replication origins throughout the cell cycle and loads other replication-initiation proteins onto replication orig...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.02.046
更新日期：2020-03-10 00:00:00
abstract：:PARP1 is the main sensor of single- and double-strand breaks in DNA and, in building chains of poly(ADP-ribose), promotes the recruitment of many downstream signaling and effector proteins involved in the DNA damage response (DDR). We show a robust physical interaction between PARP1 and the replication fork protein TI...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.09.017
更新日期：2015-10-20 00:00:00
abstract：:Stable wakefulness requires orexin/hypocretin neurons (OHNs) and OHR2 receptors. OHNs sense diverse environmental cues and control arousal accordingly. For unknown reasons, OHNs contain multiple excitatory transmitters, including OH peptides and glutamate. To analyze their cotransmission within computational framework...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.03.055
更新日期：2014-05-08 00:00:00
abstract：:We provide a catalog for the effects of the human kinome on cell survival in response to DNA-damaging agents, covering all major DNA repair pathways. By treating 313 kinase-deficient cell lines with ten diverse DNA-damaging agents, including seven commonly used chemotherapeutics, we identified examples of vulnerabilit...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.12.087
更新日期：2019-01-15 00:00:00
abstract：:Medulloblastoma (MB) is a neoplasm linked to dysregulated cerebellar development. Previously, we demonstrated that the Sonic Hedgehog (SHH) subgroup grows hierarchically, with Sox2+ cells at the apex of tumor progression and relapse. To test whether this mechanism is rooted in a normal developmental process, we studie...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.03.075
更新日期：2020-04-14 00:00:00
abstract：:α-Actinins, a family of critical cytoskeletal actin-binding proteins that usually exist as anti-parallel dimers, play crucial roles in organizing the framework of the cytoskeleton through crosslinking the actin filaments, as well as in focal adhesion maturation. However, the molecular mechanisms underlying its functio...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.11.040
更新日期：2017-12-05 00:00:00
abstract：:CXCR5 is a key marker of follicular helper T (TFH) cells. Using primary lymph nodes (LNs) from HIV-infected patients, we identified a population of CXCR5- CD4+ T cells with TFH-cell-like features. This CXCR5- subset becomes expanded in severe HIV infection and is characterized by the upregulation of activation markers...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.08.037
更新日期：2019-09-17 00:00:00
abstract：:High-throughput imaging has led to an explosion of observations about cell-size homeostasis across the kingdoms of life. Among bacteria, "adder" behavior-in which a constant size increment appears to be added during each cell cycle-is ubiquitous, while various eukaryotes show other size-homeostasis behaviors. Since in...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107992
更新日期：2020-08-11 00:00:00
abstract：:Obesity is associated with colon cancer pathogenesis, but the underlying mechanism is actively debated. Here, we confirm that diet-induced obesity promotes tumor growth in two murine colon cancer models and show that this effect is reversed by an orally administered controlled-release mitochondrial protonophore (CRMP)...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.06.008
更新日期：2018-07-03 00:00:00
abstract：:Preventing transcription-replication head-on conflict (HO-TRC)-triggered R-loop formation is essential for maintaining genome integrity in bacteria, plants, and mammals. The R-loop eraser RNase H can efficiently relax HO-TRCs. However, it is not clear how organisms resist HO-TRC-triggered R-loops when RNase H proteins...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.12.007
更新日期：2020-01-07 00:00:00
abstract：:Synaptic efficacy plays crucial roles in neuronal circuit operation and synaptic plasticity. Presynaptic determinants of synaptic efficacy are neurotransmitter content in synaptic vesicles and the number of vesicles undergoing exocytosis at a time. Bursts of presynaptic firings depress synaptic efficacy, mainly due to...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.02.080
更新日期：2018-03-20 00:00:00
abstract：:The Drosophila pharyngeal taste organs are poorly characterized despite their location at important sites for monitoring food quality. Functional analysis of pharyngeal neurons has been hindered by the paucity of molecular tools to manipulate them, as well as their relative inaccessibility for neurophysiological inves...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.11.041
更新日期：2017-12-05 00:00:00
abstract：:Evidence suggests that Polycomb (Pc) is present at chromatin loop anchors in Drosophila. Pc is recruited to DNA through interactions with the GAGA binding factors GAF and Pipsqueak (Psq). Using HiChIP in Drosophila cells, we find that the psq gene, which has diverse roles in development and tumorigenesis, encodes dist...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.07.096
更新日期：2019-09-03 00:00:00
abstract：:The transcriptional response to infection with the bacterium Listeria monocytogenes (Lm) requires cooperative signals of the type I interferon (IFN-I)-stimulated JAK-STAT and proinflammatory NF-κB pathways. Using ChIP-seq analysis, we define genes induced in Lm-infected macrophages through synergistic transcriptional ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.06.021
更新日期：2015-07-14 00:00:00
abstract：:During bacterial sepsis, proinflammatory cytokines contribute to multiorgan failure and death in a process regulated in part by cytolytic cell granzymes. When challenged with a sublethal dose of the identified mouse pathogen Brucella microti, wild-type (WT) and granzyme A (gzmA)(-/-) mice eliminate the organism from l...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.06.012
更新日期：2014-07-24 00:00:00
abstract：:Recent evidence supports the presence of an L-glutamyl methyltransferase(s) in eukaryotic cells, but this enzyme class has been defined only in certain prokaryotic species. Here, we characterize the human C6orf211 gene product as "acidic residue methyltransferase-1" (Armt1), an enzyme that specifically targets prolife...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.01.054
更新日期：2015-03-03 00:00:00
abstract：:Phenylalanine-glycine-rich nucleoporins (FG-Nups) are intrinsically disordered proteins, constituting the selective barrier of the nuclear pore complex (NPC). Previous studies showed that nuclear transport receptors (NTRs) were found to interact with FG-Nups by forming an "archetypal-fuzzy" complex through the rapid f...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.03.022
更新日期：2018-03-27 00:00:00
abstract：:Previous efforts to identify cross-neutralizing antibodies to the receptor-binding site (RBS) of ebolavirus glycoproteins have been unsuccessful, largely because the RBS is occluded on the viral surface. We report a monoclonal antibody (FVM04) that targets a uniquely exposed epitope within the RBS; cross-neutralizes E...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.04.026
更新日期：2016-05-17 00:00:00
abstract：:The pharyngeal arch arteries (PAAs) are transient embryonic blood vessels that mature into critical segments of the aortic arch and its branches. Although defects in PAA development cause life-threating congenital cardiovascular defects, the molecular mechanisms that orchestrate PAA morphogenesis remain unclear. Throu...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.07.002
更新日期：2017-07-25 00:00:00
abstract：:Telomerase biogenesis is a complex process where several steps remain poorly understood. Single-strand-selective uracil-DNA glycosylase (SMUG1) associates with the DKC1-containing H/ACA ribonucleoprotein complex, which is essential for telomerase biogenesis. Herein, we show that SMUG1 interacts with the telomeric RNA ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.07.040
更新日期：2019-08-13 00:00:00
abstract：:A rare germline duplication upstream of the bone morphogenetic protein antagonist GREM1 causes a Mendelian-dominant predisposition to colorectal cancer (CRC). The underlying disease mechanism is strong, ectopic GREM1 overexpression in the intestinal epithelium. Here, we confirm that a common GREM1 polymorphism, rs1696...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.07.020
更新日期：2014-08-21 00:00:00
abstract：:Brain function is compromised in myotonic dystrophy type 1 (DM1), but the underlying mechanisms are not fully understood. To gain insight into the cellular and molecular pathways primarily affected, we studied a mouse model of DM1 and brains of adult patients. We found pronounced RNA toxicity in the Bergmann glia of t...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.06.006
更新日期：2017-06-27 00:00:00
abstract：:Mutations of WD40-repeat protein 62 (WDR62) have been identified recently to cause human MCPH (autosomal-recessive primary microcephaly), a neurodevelopmental disorder characterized by decreased brain size. However, the underlying mechanism is unclear. Here, we investigate the function of WDR62 in brain development an...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.12.016
更新日期：2014-01-16 00:00:00
abstract：:The mammalian target of rapamycin complex 1 (mTORC1) integrates signals important for cell growth, and its dysregulation in neural stem cells (NSCs) is implicated in several neurological disorders associated with abnormal neurogenesis and brain size. However, the function of mTORC1 on NSC self-renewal and the downstre...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.09.017
更新日期：2013-10-31 00:00:00
abstract：:Antimetabolites that affect nucleotide metabolism are frontline chemotherapy agents in several cancers and often successfully target one carbon metabolism. However, the precise mechanisms and resulting determinants of their therapeutic value are unknown. We show that 5-fluorouracil (5-FU), a commonly used antimetaboli...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.05.035
更新日期：2016-06-14 00:00:00
abstract：:The bacterial pathogen Listeria monocytogenes (Lm) capitalizes on natural killer (NK) cell production of regulatory interleukin (IL)-10 to establish severe systemic infections. Here, we identify regulators of this IL-10 secretion. We show that IL-18 signals to NK cells license their ability to produce IL-10. IL-18 act...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.04.106
更新日期：2018-05-29 00:00:00
abstract：:Thymic epithelial cells (TECs) are critically required for T cell development, but the cellular mechanisms that maintain adult TECs are poorly understood. Here, we show that a previously unidentified subpopulation, EpCam(+)UEA1(-)Ly-51(+)PLET1(+)MHC class II(hi), which comprises <0.5% of adult TECs, contains bipotent ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.02.080
更新日期：2016-03-29 00:00:00
abstract：:Cell size control emerges from a regulated balance between the rates of cell growth and division. In bacteria, simple quantitative laws connect cellular growth rate to ribosome abundance. However, it remains poorly understood how translation regulates bacterial cell size and shape under growth perturbations. Here, we ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108183
更新日期：2020-09-22 00:00:00
abstract：:Amyotrophic lateral sclerosis (ALS) is an incurable neurodegenerative disease. CAV1 and CAV2 organize membrane lipid rafts (MLRs) important for cell signaling and neuronal survival, and overexpression of CAV1 ameliorates ALS phenotypes in vivo. Genome-wide association studies localize a large proportion of ALS risk va...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108456
更新日期：2020-12-01 00:00:00
abstract：:Nucleoplasmin (Npm) is an abundant histone chaperone in vertebrate oocytes and embryos. During embryogenesis, regulation of Npm histone binding is critical for its function in storing and releasing maternal histones to establish and maintain the zygotic epigenome. Here, we demonstrate that Xenopus laevis Npm post-tran...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.02.038
更新日期：2015-03-17 00:00:00
