Intercellular diffusion of a fluorescent sucrose analog via the septal junctions in a filamentous cyanobacterium.

Abstract:

UNLABELLED:Many filamentous cyanobacteria produce specialized nitrogen-fixing cells called heterocysts, which are located at semiregular intervals along the filament with about 10 to 20 photosynthetic vegetative cells in between. Nitrogen fixation in these complex multicellular bacteria depends on metabolite exchange between the two cell types, with the heterocysts supplying combined-nitrogen compounds but dependent on the vegetative cells for photosynthetically produced carbon compounds. Here, we used a fluorescent tracer to probe intercellular metabolite exchange in the filamentous heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. We show that esculin, a fluorescent sucrose analog, is incorporated by a sucrose import system into the cytoplasm of Anabaena cells. The cytoplasmic esculin is rapidly and reversibly exchanged across vegetative-vegetative and vegetative-heterocyst cell junctions. Our measurements reveal the kinetics of esculin exchange and also show that intercellular metabolic communication is lost in a significant fraction of older heterocysts. SepJ, FraC, and FraD are proteins located at the intercellular septa and are suggested to form structures analogous to gap junctions. We show that a ΔsepJ ΔfraC ΔfraD triple mutant shows an altered septum structure with thinner septa but a denser peptidoglycan layer. Intercellular diffusion of esculin and fluorescein derivatives is impaired in this mutant, which also shows a greatly reduced frequency of nanopores in the intercellular septal cross walls. These findings suggest that FraC, FraD, and SepJ are important for the formation of junctional structures that constitute the major pathway for feeding heterocysts with sucrose. IMPORTANCE:Anabaena and its relatives are filamentous cyanobacteria that exhibit a sophisticated form of prokaryotic multicellularity, with the formation of differentiated cell types, including normal photosynthetic cells and specialized nitrogen-fixing cells called heterocysts. The question of how heterocysts communicate and exchange metabolites with other cells in the filament is key to understanding this form of bacterial multicellularity. Here we provide the first information on the intercellular exchange of a physiologically important molecule, sucrose. We show that a fluorescent sucrose analog can be imported into the Anabaena cytoplasm by a sucrose import system. Once in the cytoplasm, it is rapidly and reversibly exchanged among all of the cells in the filament by diffusion across the septal junctions. Photosynthetically produced sucrose likely follows the same route from cytoplasm to cytoplasm. We identify some of the septal proteins involved in sucrose exchange, and our results indicate that these proteins form structures functionally analogous to metazoan gap junctions.

journal_name

mBio

journal_title

mBio

authors

Nürnberg DJ,Mariscal V,Bornikoel J,Nieves-Morión M,Krauß N,Herrero A,Maldener I,Flores E,Mullineaux CW

doi

10.1128/mBio.02109-14

subject

Has Abstract

pub_date

2015-03-17 00:00:00

pages

e02109

issue

2

issn

2150-7511

pii

mBio.02109-14

journal_volume

6

pub_type

杂志文章

相关文献

mBio文献大全
  • Mechanisms, Detection, and Relevance of Protein Acetylation in Prokaryotes.

    abstract::Posttranslational modification of a protein, either alone or in combination with other modifications, can control properties of that protein, such as enzymatic activity, localization, stability, or interactions with other molecules. N-ε-Lysine acetylation is one such modification that has gained attention in recent ye...

    journal_title:mBio

    pub_type: 杂志文章,评审

    doi:10.1128/mBio.02708-18

    authors: Christensen DG,Baumgartner JT,Xie X,Jew KM,Basisty N,Schilling B,Kuhn ML,Wolfe AJ

    更新日期:2019-04-09 00:00:00

  • Population Structure and Genetic Diversity among Isolates of Coccidioides posadasii in Venezuela and Surrounding Regions.

    abstract::Coccidioides posadasii is a pathogenic fungus that causes coccidioidomycosis in many arid regions of the Americas. One of these regions is bordered by the Caribbean Sea, and the surrounding landscape may play an important role in the dispersion of C. posadasii across South America through southeastern Mexico, Honduras...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.01976-19

    authors: Teixeira MM,Alvarado P,Roe CC,Thompson GR 3rd,Patané JSL,Sahl JW,Keim P,Galgiani JN,Litvintseva AP,Matute DR,Barker BM

    更新日期:2019-11-26 00:00:00

  • Association with PAK2 Enables Functional Interactions of Lentiviral Nef Proteins with the Exocyst Complex.

    abstract:UNLABELLED:Human immunodeficiency virus type 1 (HIV-1) Nef enhances virus replication and contributes to immune evasion in vivo, but the underlying molecular mechanisms remain incompletely defined. Nef interferes with host cell actin dynamics to restrict T lymphocyte responses to chemokine stimulation and T cell recept...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.01309-15

    authors: Imle A,Abraham L,Tsopoulidis N,Hoflack B,Saksela K,Fackler OT

    更新日期:2015-09-08 00:00:00

  • Petrobactin Is Exported from Bacillus anthracis by the RND-Type Exporter ApeX.

    abstract::Bacillus anthracis-a Gram-positive, spore-forming bacterium-causes anthrax, a highly lethal disease with high bacteremia titers. Such rapid growth requires ample access to nutrients, including iron. However, access to this critical metal is heavily restricted in mammals, which requires B. anthracis to employ petrobact...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.01238-17

    authors: Hagan AK,Tripathi A,Berger D,Sherman DH,Hanna PC

    更新日期:2017-09-12 00:00:00

  • An Engineered Synthetic Pathway for Discovering Nonnatural Nonribosomal Peptides in Escherichia coli.

    abstract::Peptides that are synthesized independently of the ribosome in plants, fungi, and bacteria can have clinically relevant anticancer, antihemochromatosis, and antiviral activities, among many other. Despite their natural origin, discovering new natural products is challenging, and there is a need to expand the chemical ...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.01474-17

    authors: Cleto S,Lu TK

    更新日期:2017-10-10 00:00:00

  • Engineering Kluyveromyces marxianus as a Robust Synthetic Biology Platform Host.

    abstract::Throughout history, the yeast Saccharomyces cerevisiae has played a central role in human society due to its use in food production and more recently as a major industrial and model microorganism, because of the many genetic and genomic tools available to probe its biology. However, S. cerevisiae has proven difficult ...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.01410-18

    authors: Cernak P,Estrela R,Poddar S,Skerker JM,Cheng YF,Carlson AK,Chen B,Glynn VM,Furlan M,Ryan OW,Donnelly MK,Arkin AP,Taylor JW,Cate JHD

    更新日期:2018-09-25 00:00:00

  • The pause on Avian H5N1 influenza virus transmission research should be ended.

    abstract::A voluntary 60-day pause on avian H5N1 influenza virus transmission research was announced in January 2012 by the international community of influenza scientists engaged in this work to provide time to explain the benefits of such work and the risk mitigation measures in place. Subsequently, the pause was extended to ...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.00358-12

    authors: Fouchier RA,García-Sastre A,Kawaoka Y

    更新日期:2012-11-01 00:00:00

  • Structural Analysis of the Interaction between the Bacterial Cell Division Proteins FtsQ and FtsB.

    abstract::Most bacteria and archaea use the tubulin homologue FtsZ as its central organizer of cell division. In Gram-negative Escherichia coli bacteria, FtsZ recruits cytosolic, transmembrane, periplasmic, and outer membrane proteins, assembling the divisome that facilitates bacterial cell division. One such divisome component...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.01346-18

    authors: Kureisaite-Ciziene D,Varadajan A,McLaughlin SH,Glas M,Montón Silva A,Luirink R,Mueller C,den Blaauwen T,Grossmann TN,Luirink J,Löwe J

    更新日期:2018-09-11 00:00:00

  • Supragingival Plaque Microbiome Ecology and Functional Potential in the Context of Health and Disease.

    abstract::To address the question of how microbial diversity and function in the oral cavities of children relates to caries diagnosis, we surveyed the supragingival plaque biofilm microbiome in 44 juvenile twin pairs. Using shotgun sequencing, we constructed a genome encyclopedia describing the core supragingival plaque microb...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.01631-18

    authors: Espinoza JL,Harkins DM,Torralba M,Gomez A,Highlander SK,Jones MB,Leong P,Saffery R,Bockmann M,Kuelbs C,Inman JM,Hughes T,Craig JM,Nelson KE,Dupont CL

    更新日期:2018-11-27 00:00:00

  • Murine AKAP7 has a 2',5'-phosphodiesterase domain that can complement an inactive murine coronavirus ns2 gene.

    abstract::Viral 2',5'-phosphodiesterases (2',5'-PDEs) help disparate RNA viruses evade the antiviral activity of interferon (IFN) by degrading 2',5'-oligoadenylate (2-5A) activators of RNase L. A kinase anchoring proteins (AKAPs) bind the regulatory subunits of protein kinase A (PKA) to localize and organize cyclic AMP (cAMP) s...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.01312-14

    authors: Gusho E,Zhang R,Jha BK,Thornbrough JM,Dong B,Gaughan C,Elliott R,Weiss SR,Silverman RH

    更新日期:2014-07-01 00:00:00

  • Cuts Both Ways: Proteases Modulate Virulence of Enterohemorrhagic Escherichia coli.

    abstract::Enterohemorrhagic Escherichia coli (EHEC) is a major cause of foodborne gastrointestinal illness. EHEC uses a specialized type III secretion system (T3SS) to form attaching and effacing lesions in the colonic epithelium and outcompete commensal gut microbiota to cause disease. A recent report in mBio (E. A. Cameron, M...

    journal_title:mBio

    pub_type: 评论,杂志文章

    doi:10.1128/mBio.00115-19

    authors: Palmer LD,Skaar EP

    更新日期:2019-02-26 00:00:00

  • The Peptidoglycan-Binding Protein SjcF1 Influences Septal Junction Function and Channel Formation in the Filamentous Cyanobacterium Anabaena.

    abstract:UNLABELLED:Filamentous, heterocyst-forming cyanobacteria exchange nutrients and regulators between cells for diazotrophic growth. Two alternative modes of exchange have been discussed involving transport either through the periplasm or through septal junctions linking adjacent cells. Septal junctions and channels in th...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.00376-15

    authors: Rudolf M,Tetik N,Ramos-León F,Flinner N,Ngo G,Stevanovic M,Burnat M,Pernil R,Flores E,Schleiff E

    更新日期:2015-06-30 00:00:00

  • The Reemergent 1977 H1N1 Strain and the Gain-of-Function Debate.

    abstract::The 1977-1978 influenza epidemic was probably not a natural event, as the genetic sequence of the virus was nearly identical to the sequences of decades-old strains. While there are several hypotheses that could explain its origin, the possibility that the 1977 epidemic resulted from a laboratory accident has recently...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.01013-15

    authors: Rozo M,Gronvall GK

    更新日期:2015-08-18 00:00:00

  • Membrane Distribution of the Pseudomonas Quinolone Signal Modulates Outer Membrane Vesicle Production in Pseudomonas aeruginosa.

    abstract::The Pseudomonas quinolone signal (PQS) is an important quorum-sensing molecule in Pseudomonas aeruginosa that also mediates its own packaging and transport by stimulating outer membrane vesicle (OMV) formation. Because OMVs have been implicated in many virulence-associated behaviors, it is critical that we understand ...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.01034-17

    authors: Florez C,Raab JE,Cooke AC,Schertzer JW

    更新日期:2017-08-08 00:00:00

  • Commensal Protection of Staphylococcus aureus against Antimicrobials by Candida albicans Biofilm Matrix.

    abstract::Biofilm-associated polymicrobial infections, particularly those involving fungi and bacteria, are responsible for significant morbidity and mortality and tend to be challenging to treat. Candida albicans and Staphylococcus aureus specifically are considered leading opportunistic fungal and bacterial pathogens, respect...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.01365-16

    authors: Kong EF,Tsui C,Kucharíková S,Andes D,Van Dijck P,Jabra-Rizk MA

    更新日期:2016-10-11 00:00:00

  • Characterization of an M-Cluster-Substituted Nitrogenase VFe Protein.

    abstract::The Mo- and V-nitrogenases are two homologous members of the nitrogenase family that are distinguished mainly by the presence of different heterometals (Mo or V) at their respective cofactor sites (M- or V-cluster). However, the V-nitrogenase is ~600-fold more active than its Mo counterpart in reducing CO to hydrocarb...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.00310-18

    authors: Rebelein JG,Lee CC,Newcomb M,Hu Y,Ribbe MW

    更新日期:2018-03-13 00:00:00

  • Stapled Peptides Based on Human Angiotensin-Converting Enzyme 2 (ACE2) Potently Inhibit SARS-CoV-2 Infection In Vitro.

    abstract::SARS-CoV-2 uses human angiotensin-converting enzyme 2 (ACE2) as the primary receptor to enter host cells and initiate the infection. The critical binding region of ACE2 is an ∼30-amino-acid (aa)-long helix. Here, we report the design of four stapled peptides based on the ACE2 helix, which is expected to bind to SARS-C...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.02451-20

    authors: Curreli F,Victor SMB,Ahmed S,Drelich A,Tong X,Tseng CK,Hillyer CD,Debnath AK

    更新日期:2020-12-11 00:00:00

  • The Vibrio cholerae VexGH RND Efflux System Maintains Cellular Homeostasis by Effluxing Vibriobactin.

    abstract::Resistance-nodulation-division (RND) superfamily efflux systems have been widely studied for their role in antibiotic resistance, but their native biological functions remain poorly understood. We previously showed that loss of RND-mediated efflux in Vibrio cholerae resulted in activation of the Cpx two-component regu...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.00126-17

    authors: Kunkle DE,Bina XR,Bina JE

    更新日期:2017-05-16 00:00:00

  • A Burkholderia pseudomallei colony variant necessary for gastric colonization.

    abstract:UNLABELLED:Diverse colony morphologies are a hallmark of Burkholderia pseudomallei recovered from infected patients. We observed that stresses that inhibit aerobic respiration shifted populations of B. pseudomallei from the canonical white colony morphotype toward two distinct, reversible, yet relatively stable yellow ...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.02462-14

    authors: Austin CR,Goodyear AW,Bartek IL,Stewart A,Sutherland MD,Silva EB,Zweifel A,Vitko NP,Tuanyok A,Highnam G,Mittelman D,Keim P,Schweizer HP,Vázquez-Torres A,Dow SW,Voskuil MI

    更新日期:2015-02-03 00:00:00

  • Translational Control of the SigR-Directed Oxidative Stress Response in Streptomyces via IF3-Mediated Repression of a Noncanonical GTC Start Codon.

    abstract::The major oxidative stress response in Streptomyces is controlled by the sigma factor SigR and its cognate antisigma factor RsrA, and SigR activity is tightly controlled through multiple mechanisms at both the transcriptional and posttranslational levels. Here we show that sigR has a highly unusual GTC start codon and...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.00815-17

    authors: Feeney MA,Chandra G,Findlay KC,Paget MSB,Buttner MJ

    更新日期:2017-06-13 00:00:00

  • Simple sari cloth filtration of water is sustainable and continues to protect villagers from cholera in Matlab, Bangladesh.

    abstract::A simple method for filtering water to reduce the incidence of cholera was tested in a field trial in Matlab, Bangladesh, and proved effective. A follow-up study was conducted 5 years later to determine whether the filtration method continued to be employed by villagers and its impact on the incidence of cholera. A to...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.00034-10

    authors: Huq A,Yunus M,Sohel SS,Bhuiya A,Emch M,Luby SP,Russek-Cohen E,Nair GB,Sack RB,Colwell RR

    更新日期:2010-05-18 00:00:00

  • Linkage specificity and role of properdin in activation of the alternative complement pathway by fungal glycans.

    abstract:UNLABELLED:Fungal cell walls are predominantly composed of glucans, mannans, and chitin. Recognition of these glycans by the innate immune system is a critical component of host defenses against the mycoses. Complement, an important arm of innate immunity, plays a significant role in fungal pathogenesis, especially the...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.00178-11

    authors: Agarwal S,Specht CA,Haibin H,Ostroff GR,Ram S,Rice PA,Levitz SM

    更新日期:2011-08-30 00:00:00

  • Antagonistic Rgg regulators mediate quorum sensing via competitive DNA binding in Streptococcus pyogenes.

    abstract:UNLABELLED:Recent studies have established the fact that multiple members of the Rgg family of transcriptional regulators serve as key components of quorum sensing (QS) pathways that utilize peptides as intercellular signaling molecules. We previously described a novel QS system in Streptococcus pyogenes which utilizes...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.00333-12

    authors: Lasarre B,Aggarwal C,Federle MJ

    更新日期:2013-01-02 00:00:00

  • Emergence of epidemic multidrug-resistant Enterococcus faecium from animal and commensal strains.

    abstract:UNLABELLED:Enterococcus faecium, natively a gut commensal organism, emerged as a leading cause of multidrug-resistant hospital-acquired infection in the 1980s. As the living record of its adaptation to changes in habitat, we sequenced the genomes of 51 strains, isolated from various ecological environments, to understa...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.00534-13

    authors: Lebreton F,van Schaik W,McGuire AM,Godfrey P,Griggs A,Mazumdar V,Corander J,Cheng L,Saif S,Young S,Zeng Q,Wortman J,Birren B,Willems RJ,Earl AM,Gilmore MS

    更新日期:2013-08-20 00:00:00

  • Septins arrange F-actin-containing fibers on the Chlamydia trachomatis inclusion and are required for normal release of the inclusion by extrusion.

    abstract::Chlamydia trachomatis is an obligate intracellular human pathogen that grows inside a membranous, cytosolic vacuole termed an inclusion. Septins are a group of 13 GTP-binding proteins that assemble into oligomeric complexes and that can form higher-order filaments. We report here that the septins SEPT2, -9, -11, and p...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.01802-14

    authors: Volceanov L,Herbst K,Biniossek M,Schilling O,Haller D,Nölke T,Subbarayal P,Rudel T,Zieger B,Häcker G

    更新日期:2014-10-07 00:00:00

  • Identification of Zinc-Dependent Mechanisms Used by Group B Streptococcus To Overcome Calprotectin-Mediated Stress.

    abstract::Nutritional immunity is an elegant host mechanism used to starve invading pathogens of necessary nutrient metals. Calprotectin, a metal-binding protein, is produced abundantly by neutrophils and is found in high concentrations within inflammatory sites during infection. Group B Streptococcus (GBS) colonizes the gastro...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.02302-20

    authors: Burcham LR,Le Breton Y,Radin JN,Spencer BL,Deng L,Hiron A,Ransom MR,Mendonça JDC,Belew AT,El-Sayed NM,McIver KS,Kehl-Fie TE,Doran KS

    更新日期:2020-11-10 00:00:00

  • Control of Candida albicans metabolism and biofilm formation by Pseudomonas aeruginosa phenazines.

    abstract::Candida albicans has developmental programs that govern transitions between yeast and filamentous morphologies and between unattached and biofilm lifestyles. Here, we report that filamentation, intercellular adherence, and biofilm development were inhibited during interactions between Candida albicans and Pseudomonas ...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.00526-12

    authors: Morales DK,Grahl N,Okegbe C,Dietrich LE,Jacobs NJ,Hogan DA

    更新日期:2013-01-29 00:00:00

  • Antisense transcription is pervasive but rarely conserved in enteric bacteria.

    abstract::Noncoding RNAs, including antisense RNAs (asRNAs) that originate from the complementary strand of protein-coding genes, are involved in the regulation of gene expression in all domains of life. Recent application of deep-sequencing technologies has revealed that the transcription of asRNAs occurs genome-wide in bacter...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.00156-12

    authors: Raghavan R,Sloan DB,Ochman H

    更新日期:2012-08-07 00:00:00

  • Mechanism and Function of Antiviral RNA Interference in Mice.

    abstract::Distinct mammalian RNA viruses trigger Dicer-mediated production of virus-derived small-interfering RNAs (vsiRNA) and encode unrelated proteins to suppress vsiRNA biogenesis. However, the mechanism and function of the mammalian RNA interference (RNAi) response are poorly understood. Here, we characterized antiviral RN...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.03278-19

    authors: Han Q,Chen G,Wang J,Jee D,Li WX,Lai EC,Ding SW

    更新日期:2020-08-04 00:00:00

  • Arylvinylpiperazine Amides, a New Class of Potent Inhibitors Targeting QcrB of Mycobacterium tuberculosis.

    abstract::New drugs are needed to control the current tuberculosis (TB) pandemic caused by infection with Mycobacterium tuberculosis We report here on our work with AX-35, an arylvinylpiperazine amide, and four related analogs, which are potent antitubercular agents in vitro All five compounds showed good activity against M. tu...

    journal_title:mBio

    pub_type: 杂志文章

    doi:10.1128/mBio.01276-18

    authors: Foo CS,Lupien A,Kienle M,Vocat A,Benjak A,Sommer R,Lamprecht DA,Steyn AJC,Pethe K,Piton J,Altmann KH,Cole ST

    更新日期:2018-10-09 00:00:00