Four-dimensional in vivo X-ray microscopy with projection-guided gating.

Abstract:

:Visualizing fast micrometer scale internal movements of small animals is a key challenge for functional anatomy, physiology and biomechanics. We combine phase contrast tomographic microscopy (down to 3.3 μm voxel size) with retrospective, projection-based gating (in the order of hundreds of microseconds) to improve the spatiotemporal resolution by an order of magnitude over previous studies. We demonstrate our method by visualizing 20 three-dimensional snapshots through the 150 Hz oscillations of the blowfly flight motor.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Mokso R,Schwyn DA,Walker SM,Doube M,Wicklein M,Müller T,Stampanoni M,Taylor GK,Krapp HG

doi

10.1038/srep08727

subject

Has Abstract

pub_date

2015-03-12 00:00:00

pages

8727

issn

2045-2322

pii

srep08727

journal_volume

5

pub_type

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