Abstract：
:Therapeutic strategies based on the principles of tissue engineering by self-assembly put forward the notion that functional regeneration can be achieved by utilising the inherent capacity of cells to create highly sophisticated supramolecular assemblies. However, in dilute ex vivo microenvironments, prolonged culture time is required to develop an extracellular matrix-rich implantable device. Herein, we assessed the influence of macromolecular crowding, a biophysical phenomenon that regulates intra- and extra-cellular activities in multicellular organisms, in human corneal fibroblast culture. In the presence of macromolecules, abundant extracellular matrix deposition was evidenced as fast as 48 h in culture, even at low serum concentration. Temperature responsive copolymers allowed the detachment of dense and cohesive supramolecularly assembled living substitutes within 6 days in culture. Morphological, histological, gene and protein analysis assays demonstrated maintenance of tissue-specific function. Macromolecular crowding opens new avenues for a more rational design in engineering of clinically relevant tissue modules in vitro.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kumar P,Satyam A,Fan X,Collin E,Rochev Y,Rodriguez BJ,Gorelov A,Dillon S,Joshi L,Raghunath M,Pandit A,Zeugolis DIdoi
10.1038/srep08729subject
Has Abstractpub_date
2015-03-04 00:00:00pages
8729issn
2045-2322pii
srep08729journal_volume
5pub_type
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