Abstract:
:Labeling nuclear proteins with electron dense probes in living cells has been a major challenge due to their inability to penetrate into nuclei. We developed a lipid-based approach for delivering antibodies coupled to 0.8 nm ultrasmall gold particles into the nucleus to label RNA polymerase II. Focussed Ion Beam slicing coupled to Scanning Electron Microscopy (FIB/SEM) enabled visualization of entire cells with probe localization accuracy in the 10 nm range.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Orlov I,Schertel A,Zuber G,Klaholz B,Drillien R,Weiss E,Schultz P,Spehner Ddoi
10.1038/srep08324subject
Has Abstractpub_date
2015-02-09 00:00:00pages
8324issn
2045-2322pii
srep08324journal_volume
5pub_type
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pub_type: 临床试验,杂志文章
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journal_title:Scientific reports
pub_type: 杂志文章
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journal_title:Scientific reports
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