Live cell immunogold labelling of RNA polymerase II.

Abstract:

:Labeling nuclear proteins with electron dense probes in living cells has been a major challenge due to their inability to penetrate into nuclei. We developed a lipid-based approach for delivering antibodies coupled to 0.8 nm ultrasmall gold particles into the nucleus to label RNA polymerase II. Focussed Ion Beam slicing coupled to Scanning Electron Microscopy (FIB/SEM) enabled visualization of entire cells with probe localization accuracy in the 10 nm range.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Orlov I,Schertel A,Zuber G,Klaholz B,Drillien R,Weiss E,Schultz P,Spehner D

doi

10.1038/srep08324

subject

Has Abstract

pub_date

2015-02-09 00:00:00

pages

8324

issn

2045-2322

pii

srep08324

journal_volume

5

pub_type

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