Inhibition of thermal induced protein denaturation of extract/fractions of Withania somnifera and isolated withanolides.

Abstract:

:This study describes the in vitro inhibition of protein denaturation of extract/fractions of Withania somnifera and isolated withanolides including 20β hydroxy-1-oxo(22R)-witha-2,5,24 trienolide (1), (20R,22R-14α,20α)-dihydroxy-1-oxowitha-2,5,16,24 tetraenolide (2). The results showed that the extract/fractions of the plant evoked profound inhibitory effect on thermal-induced protein denaturation. The chloroform fraction caused the most dominant attenuation of 68% at 500 μg/mL. The bioactivity-guided isolation from chloroform fraction led to the isolation of compounds 1 and 2 that showed profound protein inhibition with 78.05% and 80.43% effect at 500 μg/mL and thus strongly complimented the activity of extract/fractions. In conclusion, extract/fractions of W. somnifera possessed strong inhibition of protein denaturation that can be attributed to these isolated withanolides.

journal_name

Nat Prod Res

journal_title

Natural product research

authors

Khan MA,Khan H,Rauf A,Ben Hadda T

doi

10.1080/14786419.2015.1007138

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

2318-21

issue

24

eissn

1478-6419

issn

1478-6427

journal_volume

29

pub_type

杂志文章
  • Chemical and biological evaluation of essential oil from Saussurea costus (Falc.) Lipsch. from Garhwal Himalaya collected at different harvesting periods.

    abstract::The purpose of this study is the determination of chemical composition and antioxidant activity of essential oils (EOs) from Saussurea costus (Falc.) Lipsch. Roots have been collected on December of the first plant year (IY) and in April, August and December of the third plant year (I, 6B, D7). A GC analysis was carri...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2018.1440219

    authors: Benedetto C,D'Auria M,Mecca M,Prasad P,Singh P,Singh S,Sinisgalli C,Milella L

    更新日期:2019-08-01 00:00:00

  • Infrared spectroscopic and chemometric approach for identifying binding medium in Sukias mansion's wall paintings.

    abstract::This paper addresses the application of infrared spectroscopy in combination with chemometrics to identify wall painting's binding medium while employing pattern recognition techniques to process FTIR data-set of complex samples. In this regard, based on the historical documents and previous researches, firstly 56 sta...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2015.1108974

    authors: Haghighi Z,Karimy AH,Karami F,Bagheri Garmarudi A,Khanmohammadi M

    更新日期:2019-04-01 00:00:00

  • Lycopodiaceae from Panama: a new source of acetylcholinesterase inhibitors.

    abstract::Acetylcholinesterase (AChE) inhibitors have been used for the symptomatic treatment of Alzheimer's disease. Eleven whole plants from Panama belonging to the Lycopodiaceae family have been screened for their anticholinesterase inhibitory and antioxidant activities by a thin-layer chromatography (TLC) bioautography meth...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2012.701217

    authors: Calderón AI,Simithy-Williams J,Sanchez R,Espinosa A,Valdespino I,Gupta MP

    更新日期:2013-03-01 00:00:00

  • Novel derivatives of monascus pigment having a high CETP inhibitory activity.

    abstract::The cholesteryl ester transfer protein (CETP), inhibition of which assists in maintaining a high level of high-density lipoprotein cholesterol in the blood, is a target for anti-atherosclerosis treatments. Orange monascus pigment was produced by a Monascus species in a 5 L jar fermenter and various derivative compound...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2014.905561

    authors: Jang H,Choe D,Shin CS

    更新日期:2014-01-01 00:00:00

  • New diterpenoids from Calceolaria polifolia.

    abstract::Four new and seven known diterpenes have been isolated from the dichloromethane extract of the aerial parts of Calceolaria polifolia. The structures of the new compounds were established by spectroscopic evidence. ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410500202033

    authors: Garbarino JA,Piovano M,Chamy MC,Espinoza L

    更新日期:2007-02-01 00:00:00

  • Kitamycin C, a new antimycin-type antibiotic from Streptomyces antibioticus strain 200-09.

    abstract::An actinomycete strain 200-09, isolated from a soil sample collected from the coast of Hawaii, USA, was identified as Streptomyces antibioticus on the basis of its morphological, physiological and biochemical characteristics as well as 16S rDNA analysis. A new antimycin-type antibiotic, kitamycin C (1), together with ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2017.1295240

    authors: Wang F,Fu SN,Bao YX,Yang Y,Shen HF,Lin BR,Zhou GX

    更新日期:2017-08-01 00:00:00

  • Quaternary ammonium compounds in roots and leaves of Capparis spinosa L. from Saudi Arabia and Italy: investigation by HPLC-MS and 1H NMR.

    abstract::Capparis spinosa L. is a perennial plant typical of the Mediterranean flora and a multipurpose plant used for curing various human ailments. Quaternary ammonium compounds (QACs), as constituents of Capparaceae, play important roles in protecting against abiotic stress. Aim of this work was to determine QACs in root an...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2018.1475376

    authors: Al-Tamimi A,Khatib M,Pieraccini G,Mulinacci N

    更新日期:2019-05-01 00:00:00

  • Chemical composition, in vitro antioxidant and antibacterial activities of Centaurea resupinata subsp. dufourii (dostál) greuter.

    abstract::The current study focuses on the chemical composition, and evaluation of antioxidant and antibacterial activity of the aerial parts of Centaurea resupinata subsp. dufourii. Using different chromatographic methods nine compounds 1-9 were isolated. The structural identification of isolated compounds was achieved using s...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2020.1715397

    authors: Bouzghaia B,Moussa MTB,Goudjil R,Harkat H,Pale P

    更新日期:2020-01-23 00:00:00

  • Secondary metabolites from Garcinia daedalanthera Pierre leaves (Clusiaceae).

    abstract::Two new glycerol esters, (S)-2-hydroxy-3-(octanoyloxy)propyl tetracosanoate (1) and (S)-3-(((S)-11-acetoxy octadecanoyl)oxy)propane-1,2-diyl diacetate (2), and eight known compounds, docosanedioic acid (3), 2,5-dimethylnonadecane (4), lupeol (5), stigmasterol (6), β-sitosterol (7), heptadecanoic acid (8), hexanedioic ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2020.1777117

    authors: Forestrania RC,Anaya-Eugenio GD,Acuña UM,Ren Y,Elya B,de Blanco EC

    更新日期:2020-06-12 00:00:00

  • A new flavonoid and sulphur-containing amides from Glycosmis chlorosperma.

    abstract::A new flavonoid, dihydroglychalcone-A, was isolated from the leaves extract of Glycosmis chlorosperma in addition to two known sulphur-containing amides, dambullin and gerambullin. The structure of the new compound was assigned as 2'-hydroxy-4,6'-dimethoxy-3',4'-(2",2"-dimethylpyrano)dihydrochalcone. The extract of th...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/1057563031000122121

    authors: Rahmani M,Leng KW,Ismail HB,Hin TY,Sukari MA,Ali AM,Kulip J

    更新日期:2004-02-01 00:00:00

  • Protective effect of Epicatechin on APAP-induced acute liver injury of mice through anti-inflammation and apoptosis inhibition.

    abstract::Epicatechin (EC) is the most effective compound in Euonymus alatus (Thunb.)Sieb, and possesses a series of benefits, including anti-inflammatory, antioxidant, antiobesity and anticancer effects. In this study, we investigated the protective effects of EC in Acetaminophen(N-acetyl-p-aminophenol, APAP)-induced acute liv...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2018.1503261

    authors: Wu H,Xie Y,Xu Y,Hu Z,Wan X,Huang H,Huang D

    更新日期:2020-03-01 00:00:00

  • A new furofuran lignan from Piper terminaliflorum Tseng.

    abstract::The chemical investigation of whole plants Piper terminaliflorum Tseng led to the isolation of one new furofuran lignan, 7-methoxyasarinin (1), along with three known amide alkaloids (2-4) as N-3,5-dimethoxy-4-hydroxycinnamoylpyrrole (2), dihydropipercide (3) and 1-[(2E,4E,9E)-10-(3,4-Methylenedioxyphenyl)-2,4,9-undec...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2017.1350671

    authors: Liu T,Liang Q,Zhang XM,Huang SY,Xu WH

    更新日期:2018-02-01 00:00:00

  • Authenticating apple cider vinegar's home remedy claims: antibacterial, antifungal, antiviral properties and cytotoxicity aspect.

    abstract::These are the days when one would go online first seeking home remedies before seeing a doctor. Apple cider vinegar (ACV) is one such popular yet scientifically under-validated remedy. Our results prove the unequivocal antimicrobial activity of ACV to be true at full strength concentrations. However, the activity cann...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2017.1413567

    authors: Gopal J,Anthonydhason V,Muthu M,Gansukh E,Jung S,Chul S,Iyyakkannu S

    更新日期:2019-03-01 00:00:00

  • Identification of the phenolic compounds contributing to antibacterial activity in ethanol extracts of Brazilian red propolis.

    abstract::The purpose of this study is to identify the quantity and antibacterial activity of the individual phenolic compounds in Brazilian red propolis. Quantitative analysis of the 12 phenolic compounds in Brazilian red propolis was carried out using reversed-phase high-performance liquid chromatography. The main phenolic co...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2014.898146

    authors: Inui S,Hatano A,Yoshino M,Hosoya T,Shimamura Y,Masuda S,Ahn MR,Tazawa S,Araki Y,Kumazawa S

    更新日期:2014-01-01 00:00:00

  • Three new 3-benzylbenzofuran-2-one derivatives from Homalium brachybotrys (Flacourtiaceae/Salicaceae s. l.).

    abstract::Eight phenolic compounds, including two mixtures of two compounds, were isolated from the ethyl acetate extract of leaves and stems of Homalium brachybotrys. They were identified on the basis of spectroscopic data as quercetin-3-O-beta-glucopyranoside, luteolin-7-O-beta-glucopyranoside, 5,6-dihydro-6-beta-glucopyranos...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410601130679

    authors: Mosaddik A,Forster PI,Waterman PG

    更新日期:2007-11-01 00:00:00

  • Ascomycotin A, a new citromycetin analogue produced by Ascomycota sp. Ind19F07 isolated from deep sea sediment.

    abstract::A new citromycetin analogue, ascomycotin A (1), together with eight known compounds, wortmannilactone E (2), orcinol (3), orsellinic acid (4), isosclerone (5), (3R,4S)-( - )-4-hydroxymellein (6), diorcinol (7), chaetocyclinone B (8) and 2,5-dimethoxy-3,6-di(p-methoxypheny1)-1,4-benzoquinone (9), was isolated from the ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2014.988620

    authors: Tian YQ,Lin XP,Liu J,Kaliyaperumal K,Ai W,Ju ZR,Yang B,Wang J,Yang XW,Liu Y

    更新日期:2015-01-01 00:00:00

  • Neuroprotective effects of Sophora secundiflora, Sophora tomentosa leaves and formononetin on scopolamine-induced dementia.

    abstract::Five flavonoids were isolated from the ethyl acetate fraction of leaves of Sophora secundiflora; formononetin (1), 5-hydroxy-4'-methoxyflavone (2), genistein (3), 5-hydroxy-8-(1-hydroxy-1-methyl-ethyl)-2-(4-hydroxyphenyl)-4H-furo-[2, 3-h]-chromen-4-one (4) and ononin (5). Additionally, LC-ESI-MS/MS analysis of the eth...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2020.1795853

    authors: Aly SH,Elissawy AM,Fayez AM,Eldahshan OA,Elshanawany MA,Singab ANB

    更新日期:2020-07-22 00:00:00

  • Dragmacidoside: a new nucleoside from the Red Sea sponge Dragmacidon coccinea.

    abstract::Chemical investigation of the Red Sea sponge Dragmacidon coccinea led to the isolation of a new nucleoside, dragmacidoside (1), along with eight known compounds: adenosine (2), inosine (3), deoxycytidine (4), methyl-α-d-glucopyranoside (5), clionasterol (6), stigmasterol (7), campesterol (8) and brassicasterol (9). Th...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2014.915828

    authors: Abou-Hussein DR,Badr JM,Youssef DT

    更新日期:2014-01-01 00:00:00

  • Free radical-scavenging activities of Homalium species--An endangered medicinal plant of Eastern Ghats of India.

    abstract::Three species of the genus Homalium - e.g. Homalium nepalense, Homalium tomentosum and Homalium zeylanicum (Flacourtiaceae) - are recorded in India which are confined to the Eastern Ghat hill ranges. While H. zeylanicum is the IUCN red-listed medium-sized tree, the other two are endangered species of medicinal signifi...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2014.987142

    authors: Mahapatra AK,Pani SS,Sahoo AK

    更新日期:2015-01-01 00:00:00

  • A new androstanoid metabolite from a soil fungus Curvularia borreriae strain HS-FG-237.

    abstract::A new androstanoid metabolite, 4α-methyl-9α-methoxyandrosta-8,15-diene-3,17-dione (1), was isolated from a soil fungus Curvularia borreriae (Pleosporaceae) strain HS-FG-237. Its structure was determined by extensive spectroscopic and X-ray diffraction analyses. Compound 1 exhibited poor cytotoxicity toward HCT-116 cel...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2016.1272115

    authors: Liu LF,Zhang H,Qi H,Wang XM,Wang JD,Tan GS

    更新日期:2017-05-01 00:00:00

  • New cembranolide analogues from the formosan soft coral Sinularia flexibilis and their cytotoxicity.

    abstract::Using a bioactivity-guided fractionation procedure, five cembranolides, 11-epi-sinulariolide acetate (1), 11-dehydrosinulariolide (2), sinulariolide (3), dihydrosinularin (4), and 3,4:8,11-bisepoxy-7-acetoxycembra-15(17)-en-1,12-olide (5), along with two nucleosides, 2'-deoxyadenosine and thymidine, were isolated from...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786910310001617677

    authors: Hsieh PW,Chang FR,McPhail AT,Lee KH,Wu YC

    更新日期:2003-12-01 00:00:00

  • A new analogue of fatty alcohol from Tamarix hampeana L.

    abstract::New analogues of a long-chain secondary alcohol (1) and laserine (2) were isolated from the flowers of Tamarix hampeana L. The isolated compounds were identified using 1D and 2D NMR, LCMS/APCI, and chemical methods. Laserine was isolated for the first time from T. hampeana L. ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410802386302

    authors: Aykac A,Akgül Y

    更新日期:2010-01-01 00:00:00

  • Introduction of Adonis aestivalis as a new source of effective cytotoxic cardiac glycoside.

    abstract::Cardiac glycosides are used for treatment of irregular heartbeats, cardiac arrhythmia and congestive heart failures. In this research, digitoxin as a cardiac glycoside was identified and isolated for the first time in the world from Adonis aestivalis and investigated for its cytotoxic activity against cervical cancer ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2017.1413573

    authors: Hosseini M,Taherkhani M,Ghorbani Nohooji M

    更新日期:2019-03-01 00:00:00

  • Effect of Asteracantha longifolia seeds on the sexual behaviour of male rats.

    abstract::The aim of the present study was to study the effect of seeds of Asteracantha longifolia on the sexual behaviour of male albino rats. The ethanolic extract of seeds of A. longifolia was administered to groups of rats in 100, 150 and 200 mg kg⁻¹ doses for a period of 28 days, and the action compared with control rats. ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410802588493

    authors: Chauhan NS,Sharma V,Dixit VK

    更新日期:2011-09-01 00:00:00

  • Repellence of the main components from the essential oil of Glycosmis lucida Wall. ex Huang against two stored product insects.

    abstract::A screening of Chinese medicinal herbs and wild plants for agrochemicals was carried out; the essential oil of Glycosmis lucida leaves was found to possess significant repellent activity against Tribolium castaneum and Liposcelis bostrychophila. It was found that the main components included elixene (19.81%), spathule...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2016.1226825

    authors: Guo SS,Zhang WJ,Yang K,Liang JY,You CX,Wang CF,Li YP,Geng ZF,Deng ZW,Du SS

    更新日期:2017-05-01 00:00:00

  • A new lupane-type triterpenoid fatty acid ester and other isolates from Ophiorrhiza shendurunii.

    abstract::A new pentacyclic triterpenoid fatty acid ester, lupan-20-ol-3(β)-yl hexadecanoate (1), together with lupan-20-ol-3(β)-yl acetate (2), olean-18-en-3(β)-yl hexadecanoate (3), dotriacontanoic acid (4), stigmasterol (5), rubiadin (6), nonadecanoic acid (7), palmitic acid (8) and camptothecin (9) were isolated from the he...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2016.1160232

    authors: Rajan R,Venkataraman R,Baby S

    更新日期:2016-10-01 00:00:00

  • β-Hydroxydihydrochalcone and flavonoid glycosides along with triterpene saponin and sesquiterpene from the herbs of Pimpinella rhodantha Boiss.

    abstract::A new β-hydroxydihydrochalcone glycoside named ziganin (1) and a new acylated flavonol glycoside named isorhamnetin-3-O-α-L-(2″,3″-di-O-trans-coumaroyl)-rhamnopyranoside) (2), along with two known flavonoid glycosides, a β-hydroxydihydrochalcone glycoside, a hydroxybenzoic acid derivative, a trinorguaiane type sesquit...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2015.1062006

    authors: Özbek H,Güvenalp Z,Kuruüzüm-Uz AE,Kazaz C,Demirezer LÖ

    更新日期:2016-01-01 00:00:00

  • Bioactive terpenoids from the Red Sea soft coral Sinularia polydactyla.

    abstract::Chemical investigation of an ethyl acetate soluble fraction of Sinularia polydactyla (Ehrenberg) led to the isolation of three known terpenoides, two of them sterols, 24-methylcholestane-3β,5α,6β,25-tetrol 25-monoacetate (1), 24-methylcholestane-5-en-3β,25-diol (2), in addition to a cembranoid diterpene, durumolide C ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2013.805333

    authors: Aboutabl el-SA,Azzam SM,Michel CG,Selim NM,Hegazy MF,Ali AH,Hussein AA

    更新日期:2013-01-01 00:00:00

  • Chemistry of α-mangostin. Studies on the semisynthesis of minor xanthones from Garcinia mangostana.

    abstract::α-Mangostin is the major prenylated xanthone from Garcinia mangostana and it has been used also in recent times as starting material for the semisynthetic preparation of various biologically active derivatives. Its structure is characterised by the presence of few functional groups amenable to chemical manipulations, ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2014.986729

    authors: Morelli CF,Biagiotti M,Pappalardo VM,Rabuffetti M,Speranza G

    更新日期:2015-01-01 00:00:00

  • Antioxidant activity and cytotoxicity on tumour cells of the essential oil from Cedronella canariensis var. canariensis (L.) Webb & Berthel. (Lamiaceae).

    abstract::Cedronella canariensis is a lemon-scented species of the family Lamiaceae endemic to the Canary Islands where it is used in the traditional medicine to prepare infusions or inhalations for anti-catarrhal, tonic, diuretic, hypoglycaemiant, hypotensive, anti-inflammatory and decongestant of the respiratory tract. In thi...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2014.994213

    authors: Zorzetto C,Sánchez-Mateo CC,Rabanal RM,Lupidi G,Bramucci M,Quassinti L,Iannarelli R,Papa F,Maggi F

    更新日期:2015-01-01 00:00:00